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1دورية أكاديمية
المؤلفون: Joanna Wielińska, Katerina Tarassi, Milena Iwaszko, Katarzyna Kościńska, Barbara Wysoczańska, Evangelia Mole, Vasiliki Kitsiou, Jerzy Świerkot, Katarzyna Kolossa, Diamanto Kouniaki, Theofilos Athanassiades, Alexandra Tsirogianni, Katarzyna Bogunia-Kubik
المصدر: Central European Journal of Immunology, Vol 46, Iss 1, Pp 92-98 (2021)
مصطلحات موضوعية: hla-drb1 shared epitope, nkg2d, mica, polymorphism, rheumatoid arthritis., Medicine
الوصف: The present study aimed to analyse and compare the distribution of MICA (rs1051792) and NKG2D/KLRK1 (rs1154831, rs1049174, rs2255336) polymorphisms in 61 Greek and 100 Polish patients with rheumatoid arthritis in relation to the presence of the HLA-DRB1 shared epitope and clinical parameters. Genotyping of selected polymorphism was performed using real-time PCR. HLA-DRB1 shared epitope alleles segregated differently in Greek and Polish patients but in both populations were detected in over 60% of cases. The rs1051792-A variant was more common among SE-positive Polish patients (p = 0.003) while the rs1049174-G allele was more frequently observed in Greeks than in Poles (p < 0.001). Moreover, among Greek patients, the rs1051792-GG homozygotes more frequently presented with anti-CCP antibodies and rheumatoid factor (RF), while carriers of the rs1049174-G variant and rs1154831-CC homozygotes were characterized by lower disease activity scores (p < 0.05 in all cases). These results imply that, in addition to the HLA-DRB1 SE alleles, MICA and NKG2D polymorphisms may also play a role in rheumatoid arthritis.
وصف الملف: electronic resource
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2دورية أكاديمية
المؤلفون: Sylwia Biały, Milena Iwaszko, Jerzy Świerkot, Bartosz Bugaj, Katarzyna Kolossa, Sławomir Jeka, Katarzyna Bogunia-Kubik
المصدر: International Journal of Molecular Sciences, Vol 23, Iss 21, p 13177 (2022)
مصطلحات موضوعية: interleukin 5, interleukin 9, IL5, IL9, ankylosing spondylitis, anti-TNF, Biology (General), QH301-705.5, Chemistry, QD1-999
الوصف: Ankylosing spondylitis (AS) is an inflammatory disease that belongs to the spondyloarthritis family. IL-5 and IL-9 belong to the group of Th2 cytokines of anti-inflammatory nature. Polymorphisms in their coding genes have been so far associated with various inflammatory diseases, but there are no reports regarding their involvement in AS pathogenesis to date. The purpose of the study was to investigate relationships between IL5 and IL9 genetic variants with AS susceptibility, clinical parameters as well as response to therapy with TNF inhibitors. In total 170 patients receiving anti-TNF therapy and 218 healthy controls were enrolled in the study. The genotyping of IL5 rs2069812 (A > G) and IL9 rs2069885 (G > A) single nucleotide polymorphisms was performed using the Real-Time PCR method based on LightSNiP kits assays. The present study demonstrated significant relationships between IL5 rs2069812 and IL9 rs2069885 polymorphisms and response to anti-TNF therapy. Presence of the IL5 rs2069812 A allele in patients positively correlated with better response to treatment (p = 0.022). With regard to IL9 rs2069885, patients carrying the A allele displayed better outcomes in anti-TNF therapy (p = 0.046). In addition, IL5 rs2069812 A and IL9 rs2069885 A alleles were associated with lower CRP and VAS values. The obtained results may indicate a significant role for IL-5 and IL-9 in the course of AS and response to anti-TNF therapy.
وصف الملف: electronic resource
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3دورية أكاديمية
المؤلفون: Milena Iwaszko, Joanna Wielińska, Jerzy Świerkot, Katarzyna Kolossa, Renata Sokolik, Bartosz Bugaj, Monika Chaszczewska-Markowska, Sławomir Jeka, Katarzyna Bogunia-Kubik
المصدر: Frontiers in Immunology, Vol 12 (2021)
مصطلحات موضوعية: rheumatoid arthritis, psoriatic arthritis, ankylosing spondylitis, spondyloarthritis, IL-33 gene polymorphism, anti-TNF therapy, Immunologic diseases. Allergy, RC581-607
الوصف: ObjectiveRheumatoid arthritis (RA), ankylosing spondylitis (AS), and psoriatic arthritis (PsA) belong to inflammatory rheumatic diseases, the group of conditions of unknown etiology. However, a strong genetic component in their pathogenesis has been well established. A dysregulation of cytokine networks plays an important role in the development of inflammatory arthritis. Interleukin 33 (IL-33) is a recently identified member of the IL-1 family. To date, the significance of IL-33 in inflammatory arthritis has been poorly studied. This research aimed to investigate the potential of IL-33 gene polymorphisms to serve as biomarkers for disease susceptibility and TNF inhibitor response in RA, AS, and PsA patients.Materials and MethodsIn total, 735 patients diagnosed with RA, AS, and PsA and 229 healthy individuals were enrolled in the study. Genotyping for three single nucleotide polymorphisms (SNPs) within the IL-33 gene, namely, rs16924159 (A/G), rs10975519 (T/C), and rs7044343 (C/T), was performed using polymerase chain reaction amplification employing LightSNiP assays.ResultsIn the present study, the IL-33 rs10975519 CC genotype was associated with a decreased risk of developing RA in females, while the IL-33 rs16924159 polymorphism was associated with the efficacy of anti-TNF therapy and clinical parameters for RA and AS patients. The IL-33 rs16924159 AA genotype correlated with higher disease activity and worse clinical outcomes in RA patients treated with TNF inhibitors, and AS patients carrying the IL-33 rs16924159 AA genotype had higher disease activity and a worse response to anti-TNF therapy. That indicates a deleterious role of the IL-33 rs16924159 AA genotype in the context of RA, as well as AS.ConclusionsThe obtained results suggest that IL-33 gene polymorphisms might be potential candidate biomarkers of disease susceptibility and anti-TNF treatment response in patients with inflammatory rheumatic diseases.
وصف الملف: electronic resource
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4دورية أكاديمية
المؤلفون: Milena Iwaszko, Sylwia Biały, Katarzyna Bogunia-Kubik
المصدر: Cells, Vol 10, Iss 11, p 3000 (2021)
مصطلحات موضوعية: inflammatory arthritis, rheumatoid arthritis, psoriatic arthritis, ankylosing spondylitis, spondyloarthritis, IL-4, Cytology, QH573-671
الوصف: Interleukin (IL)-4 and IL-13 belong to the T helper 2 (Th2) cytokine family, along with IL-3, IL-5, and IL-9. These cytokines are key mediators of allergic inflammation. They have important immunomodulatory activities and exert influence on a wide variety of immune cells, such as B cells, eosinophils, basophils, monocytes, fibroblasts, endothelial cells, airway epithelial cells, smooth muscle cells, and keratinocytes. Recent studies have implicated IL-4 and IL-13 in the development of various autoimmune diseases. Additionally, these cytokines have emerged as potential players in pathogenesis of inflammatory arthritis. Recent findings suggest that the IL-4 and IL-13 might play a significant role in the downregulation of inflammatory processes underlying RA pathology, and beneficially modulate the course of the disease. This review summarizes the biological features of the IL-4 and IL-13 and provides current knowledge regarding the role of these cytokines in inflammatory arthritis.
وصف الملف: electronic resource
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5دورية أكاديمية
المؤلفون: Milena Iwaszko, Jerzy Świerkot, Katarzyna Kolossa, Sławomir Jeka, Piotr Wiland, Katarzyna Bogunia-Kubik
المصدر: Genes, Vol 9, Iss 2, p 94 (2018)
الوصف: It has been brought to our attention that the funding of the National Center of Science (Poland) was missing in the acknowledgements section of our published paper [1], and therefore we would like to add this and report the acknowledgements as follows:[...]
وصف الملف: electronic resource
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6دورية أكاديمية
المؤلفون: Milena Iwaszko, Jerzy Świerkot, Katarzyna Kolossa, Sławomir Jeka, Piotr Wiland, Katarzyna Bogunia-Kubik
المصدر: Genes, Vol 9, Iss 2, p 64 (2018)
مصطلحات موضوعية: NKG2D polymorphism, anti-TNF therapy, TNF inhibitors, Genetics, QH426-470
الوصف: A natural killer group 2 member D (NKG2D) acts as a powerful activating and co-stimulatory receptor on immune effector cells including NK and T cells. Disruptions within the NKG2D signalling pathway may trigger an exacerbated immune response and promote autoimmune reactions. The objective of the study was to evaluate a plausible role of polymorphisms within the NKG2D gene as a predictor of how effective anti-tumor necrosis factor (TNF) therapy is in rheumatoid arthritis (RA) patients. A total of 280 RA patients receiving anti-TNF therapy were genotyped for NKG2D rs2255336 (A > G), rs1049174 (C > G), and rs1154831 (C > A). Clinical response was evaluated according to the European League against Rheumatism (EULAR) criteria at the 12th and 24th week. Both the NKG2D rs225336 and rs1049174 polymorphisms were significantly associated with efficacy of TNF inhibitors. Inefficient therapy was more frequently observed in patients with rs2255336 GG or rs1049174 CC genotype as compared to other genotypes (p-value = 0.003 and p-value = 0.004, respectively). The presence of the rs2255336 G or the rs1049174 C allele correlated with a worse EULAR response (p-value = 0.002, p-value = 0.031, respectively). Moreover, patients carrying the rs2255336 or rs1049174 heterozygous genotype achieved better EULAR responses than patients with homozygous genotypes (p-value = 0.010 and p-value = 0.002, respectively). Data from the present study provides evidence that NKG2D polymorphisms may affect response to anti-TNF inhibitors in RA patients.
وصف الملف: electronic resource
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المؤلفون: Katarzyna Bogunia-Kubik, Renata Sokolik, Lucyna Korman, Piotr Wiland, Jerzy Świerkot, Milena Iwaszko, Barbara Wysoczańska
المصدر: Pharmacogenomics and Personalized Medicine
مصطلحات موضوعية: 0301 basic medicine, medicine.medical_specialty, IL-6R Asp358Ala polymorphism, Single-nucleotide polymorphism, Gastroenterology, 03 medical and health sciences, Psoriatic arthritis, 0302 clinical medicine, Pharmacogenomics and Personalized Medicine, Polymorphism (computer science), Internal medicine, Genotype, Medicine, SNP, Allele, Interleukin 6, Original Research, psoriatic arthritis, Pharmacology, IL-6 −174G/C polymorphism, biology, business.industry, medicine.disease, 030104 developmental biology, 030220 oncology & carcinogenesis, methotrexate treatment, biology.protein, Molecular Medicine, Methotrexate, business, medicine.drug
الوصف: Renata Sokolik,1,* Milena Iwaszko,2,* Jerzy Åwierkot,1 Barbara WysoczaÅska,2 Lucyna Korman,1 Piotr Wiland,1 Katarzyna Bogunia-Kubik2 1Department of Rheumatology and Internal Medicine, WrocÅaw Medical University, WrocÅaw, Poland; 2Laboratory of Clinical Immunogenetics and Pharmacogenetics, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, WrocÅaw, Poland*These authors contributed equally to this workCorrespondence: Milena IwaszkoHirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, WrocÅaw 53-114, PolandTel +48 71 3709960Fax +48 71 3371382Email milena.iwaszko@hirszfeld.plIntroduction: The purpose of the study was to investigate whether single-nucleotide polymorphisms (SNPs) IL-6 − 174 G/C and IL-6R Asp358Ala are associated with susceptibility to psoriatic arthritis (PsA) or affect response to treatment with methotrexate (MTX).Patients and Methods: Seventy-four patients diagnosed with PsA and qualified for MTX treatment were enrolled to the study. The control group consisted of 120 healthy individuals. Polymorphisms IL-6 − 174 G/C and IL-6R Asp358Ala were genotyped using a polymerase chain reaction (PCR) amplification employing LightSNiP assays.Results: A significant association between the IL-6 − 174 CC genotype and an improved clinical outcome of MTX therapy was observed. A good response was more frequently observed among PsA patients bearing the IL-6 − 174 CC genotype than patients with the GC or GG genotypes (P = 0.007). On the other hand, patients carrying the IL-6 − 174 GC genotype less frequently responded to MTX treatment as compared to patients with other genotypes (P = 0.006). With respect to the IL-6R Asp358Ala SNP, there were no significant differences in genotype and allelic frequencies in relation to clinical outcome of MTX treatment. No association was found between the IL-6 − 174 G/C or IL-6R Asp358Ala SNPs and PsA susceptibility.Conclusion: Results from this study provide evidence that the IL-6 − 174 G/C polymorphism might influence efficacy of MTX treatment.Keywords: psoriatic arthritis, methotrexate treatment, IL-6 − 174G/C polymorphism, IL-6R Asp358Ala polymorphism
وصف الملف: text/html
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المؤلفون: Sylwia Biały, Katarzyna Bogunia-Kubik, Milena Iwaszko
المصدر: Cells, Vol 10, Iss 3000, p 3000 (2021)
Cellsمصطلحات موضوعية: rheumatoid arthritis, QH301-705.5, Inflammatory arthritis, medicine.medical_treatment, Review, Bone and Bones, Allergic inflammation, Psoriatic arthritis, Immune system, ankylosing spondylitis, medicine, Animals, Humans, Biology (General), Interleukin 4, inflammatory arthritis, Inflammation, psoriatic arthritis, Interleukin-13, business.industry, Arthritis, IL-4, Interleukin, General Medicine, spondyloarthritis, medicine.disease, Disease Models, Animal, Cytokine, IL-13, Immunology, Interleukin 13, Interleukin-4, business
الوصف: Interleukin (IL)-4 and IL-13 belong to the T helper 2 (Th2) cytokine family, along with IL-3, IL-5, and IL-9. These cytokines are key mediators of allergic inflammation. They have important immunomodulatory activities and exert influence on a wide variety of immune cells, such as B cells, eosinophils, basophils, monocytes, fibroblasts, endothelial cells, airway epithelial cells, smooth muscle cells, and keratinocytes. Recent studies have implicated IL-4 and IL-13 in the development of various autoimmune diseases. Additionally, these cytokines have emerged as potential players in pathogenesis of inflammatory arthritis. Recent findings suggest that the IL-4 and IL-13 might play a significant role in the downregulation of inflammatory processes underlying RA pathology, and beneficially modulate the course of the disease. This review summarizes the biological features of the IL-4 and IL-13 and provides current knowledge regarding the role of these cytokines in inflammatory arthritis.
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المؤلفون: Jerzy Świerkot, Joanna Wielińska, Katarzyna Bogunia-Kubik, Marta Dratwa, Barbara Wysoczańska, Katarzyna Kolossa, Monika Chaszczewska-Markowska, Sławomir Jeka, Bartosz Bugaj, Milena Iwaszko
المصدر: Archivum Immunologiae et Therapiae Experimentalis
مصطلحات موضوعية: Adult, Male, medicine.medical_specialty, Genotype, Immunology, Single-nucleotide polymorphism, Disease, RANK, Polymorphism, Single Nucleotide, Gastroenterology, Arthritis, Rheumatoid, 03 medical and health sciences, 0302 clinical medicine, Gene Frequency, Internal medicine, Humans, Immunology and Allergy, Medicine, Genetic Predisposition to Disease, Genetic variability, Polymorphism, Rheumatoid arthritis, Allele, Receptor, Genetic Association Studies, Aged, 030304 developmental biology, 0303 health sciences, Receptor Activator of Nuclear Factor-kappa B, biology, business.industry, RANK Ligand, RANKL, Anti-TNF therapy, General Medicine, Middle Aged, medicine.disease, Treatment Outcome, 030220 oncology & carcinogenesis, Disease Progression, biology.protein, Alkaline phosphatase, Original Article, Female, Tumor Necrosis Factor Inhibitors, Immunotherapy, business
الوصف: Inconsistency of the results regarding the genetic variability within genes coding for receptor activator of nuclear factor κB (RANK) and its ligand (RANKL) in rheumatoid arthritis (RA) prompted us to study the RANK and RANKL polymorphisms as potential biomarkers associated with disease predisposition and response to anti-TNF treatment in a group of Polish patients with RA. This study enrolled 318 RA patients and 163 controls. RANK (rs8086340, C > G; rs1805034, C > T) and RANKL (rs7325635, G > A; rs7988338 G > A) alleles were determined by real-time PCR with melting curve analysis and related with clinical parameters. In addition, RANKL serum levels were measured by ELISA. The RANK rs8086340-G allele was overrepresented among patients as compared to controls (OD = 1.777, p = 0.038). C-reactive protein (CRP) levels were significantly (p RANK rs8086340 polymorphism and were higher in the CC-homozygotes at the baseline while lower in the GG-carriers at the 12th week of the treatment. At the latter time point RANKL rs7325635-GG-positive patients also showed significantly lower CRP concentrations. Higher alkaline phosphatase levels before induction of anti-TNF therapy were observed in RANK rs8086340 and RANK rs1805034 CC homozygotes (p = 0.057 and p = 0.035, respectively). The GG homozygosity of both RANKL single nucleotide polymorphisms was significantly associated with the number of swollen joints (rs7988338 and rs7325635, before and at the 12th week of therapy, respectively, p RANK and RANKL genes affect RA susceptibility and anti-TNF treatment outcome.
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5936e1013aa5b364d89bb778b6b8f45e
https://doi.org/10.1007/s00005-020-00590-6 -
10
المؤلفون: Joanna, Wielińska, Katerina, Tarassi, Milena, Iwaszko, Katarzyna, Kościńska, Barbara, Wysoczańska, Evangelia, Mole, Vasiliki, Kitsiou, Jerzy, Świerkot, Katarzyna, Kolossa, Diamanto, Kouniaki, Theofilos, Athanassiades, Alexandra, Tsirogianni, Katarzyna, Bogunia-Kubik
المصدر: Central-European Journal of Immunology
مصطلحات موضوعية: musculoskeletal diseases, rheumatoid arthritis, MICA, HLA-DRB1 shared epitope, Clinical Immunology, humanities, NKG2D, polymorphism
الوصف: The present study aimed to analyse and compare the distribution of MICA (rs1051792) and NKG2D/KLRK1 (rs1154831, rs1049174, rs2255336) polymorphisms in 61 Greek and 100 Polish patients with rheumatoid arthritis in relation to the presence of the HLA-DRB1 shared epitope and clinical parameters. Genotyping of selected polymorphism was performed using real-time PCR. HLA-DRB1 shared epitope alleles segregated differently in Greek and Polish patients but in both populations were detected in over 60% of cases. The rs1051792-A variant was more common among SE-positive Polish patients (p = 0.003) while the rs1049174-G allele was more frequently observed in Greeks than in Poles (p < 0.001). Moreover, among Greek patients, the rs1051792-GG homozygotes more frequently presented with anti-CCP antibodies and rheumatoid factor (RF), while carriers of the rs1049174-G variant and rs1154831-CC homozygotes were characterized by lower disease activity scores (p < 0.05 in all cases). These results imply that, in addition to the HLA-DRB1 SE alleles, MICA and NKG2D polymorphisms may also play a role in rheumatoid arthritis.
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