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1دورية أكاديمية
المؤلفون: F. N. Paleev, E. I. Ostrovskiy, V. I. Karandashov, S. N. Shatokhina, N. P. Sanina, O. Yu. Ryzhkova, E. M. Gorbunova, N. R. Paleev
المصدر: Alʹmanah Kliničeskoj Mediciny, Vol 0, Iss 43, Pp 58-65 (2016)
مصطلحات موضوعية: blood rheology, bronchial asthma, photohaemotherapy, Medicine
الوصف: Background: Blood rheology abnormalities are recognized as one of the main pathophysiological components of bronchial asthma. Standard medical treatment of bronchial asthma does not exert any substantial effect on blood fluidity. Aim: To assess efficacy of quantum haemotherapy and its influence on blood rheology in the combination treatment of bronchial asthma.Materials and methods: Four hundred and eighty patients admitted to our in-patient department with mild (44.5%) and severe (55.5%) exacerbations of persistent moderate asthma, were randomized into two groups: the main group (n = 250), which, along with conventional treatment, received photohaemotherapy with blue (PHB, n = 220) or red (PHR, n = 30) light, and the control group (n = 230), which received conventional treatment for two weeks. PHB (light-emitting diode devise “Solaris”) and PHR (helium neon laser) was performed according to the following regimen: duration of a session 30 minutes, number of sessions, from 5 to 7 with in-between intervals of 2 to 3 days. The degree of erythrocyte deformation, relative membrane charge and relative gradient membrane potential of erythrocytes, total erythrocyte counts, hemoglobin levels, hematocrit, and blood viscosity were assessed at days 1 and 14, as well as after 3 months.Results: At day 14, the relative gradient membrane potential of erythrocytes decreased to 0.118 ± 0.01 r.u. in the PHB group, to 0.167 ± 0.01 r.u. in the PHR group, and to 0.153 ± 0.01 r.u. in the conventional treatment group. The relative charge of erythrocyte membranes increased to 0.202 ± 0.07, 0.19 ± 0.07 and 0.14 ± 0.07 r.u., the degree of erythrocyte deformation decreased to 1.4 ± 0.1, 1.6 ± 0.1 and 1.9 ± 0.1 scores, the degree of hypoxic anisotropy of the serum decreased to 0.8, 0.9 and 1.2 scores, respectively (in all cases, p < 0.05 for comparison with baseline values). However, a significant difference of all parameters from the normal range persisted (p < 0.001). No significant changes of hemoglobin and total erythrocyte counts were observed during the whole follow up period. At the end of the photohaemotherapy course, blood viscosity in the PHB group decreased to 4.55 ± 0.11 mPa·s (from the baseline value of 5.04 ± 0.13 mPa·s, p < 0.05), and was close to normal range (4.5 ± 0.3 mPa·s). In the conventionally treated group, blood viscosity was not changed (5.41 ± 0.11 mPa·s before treatment and 5.39 ± 0.12 mPa·s thereafter, p > 0.05). At month 3, all patients, despite their clinical well-being, demonstrated a deterioration of the parameters studies. However, only in the conventionally treated group the degree of this deterioration was compatible with parameters registered during an asthma exacerbation.Conclusion: Blood rheology parameters demonstrated a more rapid and advanced improvement in patients with exacerbations of bronchial asthma, who were treated with the use of photohaemotherapy, compared to those who received conventional treatment only.
وصف الملف: electronic resource
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2دورية أكاديمية
المؤلفون: E. I. Ostrovskiy, V. I. Karandashov, S. N. Shatokhina, N. P. Sanina, O. Yu. Ryzhkova, E. M. Katlomina, N. R. Paleev
المصدر: Alʹmanah Kliničeskoj Mediciny, Vol 0, Iss 35, Pp 60-65 (2016)
مصطلحات موضوعية: bronchial asthma, photohemotherapy, clinical parameters, effectiveness, Medicine
الوصف: Background: Anti-inflammatory and immunoregulatory effects of photohemotherapy have been demonstrated in myocarditis, chronic obstructive pulmonary disease, some autoimmune and other disorders. There are only limited reports on the use of photohemotherapy in bronchial asthma (BA). Aim: To assess the influence of blue- and red-light photohemotherapy on clinical course of BA. Materials and methods: 480 hospitalized patients with persistent moderate BA and mild (44.5%) or moderate (55.5%) exacerbation were randomized to receive standard therapy of asthma exacerbation and photohemotherapy (treatment group, n=250) or standard therapy only (control group, n=230). Blue-light photohemotherapy (photodiode apparatus Solaris, n=220) or redlight photohemotherapy (helium-neon gas laser, n=30) was applied according to the schedule: procedure duration – 30 minutes, number of sessions – 5–7, every 2–3 days. Results: In asthma exacerbation, adding photohemotherapy to standard treatment was associated with more rapid improvement of clinical symptoms: maximal total asthma scores were achieved by the day 7–10 in patients with mild exacerbation and by the day 10–14 in patients with moderate exacerbation. Therapeutic effects of blue-light photohemotherapy were superior to those of red-light photohemotherapy. The data of 3-year follow-up supported the role of photohemotherapy in achieving asthma control: in the treatment group, proportion of patients with total control of asthma was 1.3-fold higher and one-year hospitalization number was lower by 34.8% compared with the controls. Conclusion: Blue-light and red-light photohemotherapy positively influences the clinical course of asthma and is associated with faster remission achievement and reduction of duration and frequency of hospitalizations.
وصف الملف: electronic resource
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3دورية أكاديمية
المؤلفون: F. N. Paleev, N. P. Sanina, A. I. Makarkov, N. M. Mylov, E. I. Ostrovskiy, N. N. Khishova, O. V. Moskalets, N. R. Paleev
المصدر: Alʹmanah Kliničeskoj Mediciny, Vol 0, Iss 35, Pp 12-21 (2016)
مصطلحات موضوعية: infectious immune myocarditis, post-myocarditis cardiosclerosis, lymphocytes, immune activation, Medicine
الوصف: Background: Autoantibodies to myocardial antigenic epitopes and corresponding autoreactive T cell clones play an important role in myocardial damage and in pathogenesis of infectious immune myocarditis. T lymphocytes subpopulations/regulatory T cells balance as well as T cell-derived cytokines are crucial in the mechanisms of immune regulation in myocarditis. Aim: To assess quantitative parameters and functional characteristics of basic peripheral blood lymphocytes subpopulations in patients with infectious immune myocarditis and post-myocarditis cardiosclerosis. Material and methods: 35 patients with infectious immune myocarditis and 39 patients with post-myocarditis cardiosclerosis were included. Among infectious immune myocarditis patients, 17 patients had advanced congestive heart failure (CHF) (NYHA III), and 18 patients had mild, moderate or no heart failure (NYHA 0-II). Among cardiosclerosis patients, 18 patients had no CHF, and 21 had only mild symptoms of heart failure (NYHA I). 10 healthy volunteers were enrolled in the control group. Populations and subpopulations of peripheral blood lymphocytes and markers of lymphocytes activation were studied using quadricolor laser flow cytometry (FACSCalibur) and suitable monoclonal antibodies (Becton Dickinson, USA). Results: Inflammatory myocardial diseases are characterized by alterations of innate and adaptive immunity. In our study, patients with infectious immune myocarditis and post-myocarditis cardiosclerosis had significantly reduced numbers of natural killer T cells irrespective of CHF symptoms and disease duration. T cell immunity disturbances were characterized by decreased numbers of CD3+CD4+ cells depending from the disease duration and symptoms severity. Patients with infectious immune myocarditis also had increased numbers of B cells. Immune activation was demonstrated both in infectious immune myocarditis and (less prominent) in post-myocarditis cardiosclerosis. Increased expression of early activation marker CD25 was found during the first 2 weeks from the disease onset in patients with infectious immune myocarditis. In 1 month and during the second month of the disease, increased numbers of T cells and non-T lymphocytes were demonstrated along with late activation manifested by the expression of HLA-DR antigen. Different severity of CHF symptoms was associated with different patterns of activation markers. Increased expression of apoptosis marker CD95 was found in both infectious immune myocarditis and post-myocarditis cardiosclerosis; maximal CD95 values were demonstrated in myocarditis patients in 1 month after the disease onset. Conclusion: Disturbances of anti-infection immunity and self-limitation mechanisms of immune reactions play an important role in the development and progression of inflammatory myocardial diseases.
وصف الملف: electronic resource
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4دورية أكاديمية
المؤلفون: N. R. Paleev, F. N. Paleev
المصدر: Alʹmanah Kliničeskoj Mediciny, Vol 0, Iss 35, Pp 6-11 (2016)
مصطلحات موضوعية: Medicine
وصف الملف: electronic resource
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5دورية أكاديمية
المؤلفون: F. N. Paleev, E. I. Ostrovskiy, V. I. Karandashov, S. N. Shatokhina, N. P. Sanina, O. Yu. Ryzhkova, E. M. Gorbunova, N. R. Paleev
المصدر: Alʹmanah Kliničeskoj Mediciny, Vol 0, Iss 39, Pp 104-110 (2016)
مصطلحات موضوعية: bronchial asthma, photohemotherapy, immunological parameters, efficacy, Medicine
الوصف: Background: Photohemotherapy combined with conventional therapy can increase the treatment efficacy in asthma. However, the influence of quantumhemotherapy on pathogenetic mechanisms of the disease has been poorly studied.Aim: To evaluate the influence of blue and red light hemotherapy on blood immunological parаmeters in asthma patients.Materials and methods: Four hundred and eighty patients admitted to the in-patient department with mild (44.5%) and severe (55.5%) exacerbations of persistent moderate asthma, were randomized into two groups: the main group (n = 250), which, along with conventional treatment, received photohemotherapy with blue (n = 220) or red (n = 30) light, and the control group (n = 230), which received conventional treatment only. Before and after treatment, immunological assessments were performed that included phenotyping of the lymphocyte subpopulations (CD3, CD4, CD8, CD16, CD19, CD56), assessment of phagocytal activity of leukocytes, measurement of circulating immune complexes (CIC) and immunoglobulin levels (IgE, IgG, IgM and IgA).Results: After combined treatment, compared to baseline values, patients from the main group had a significant increase in СD3+-lymphocytes (from 32.34 ± 2.28 to 60.73 ± 0.67%) and their immunoregulatory subpopulations – СD4+-helpers (from 17.94 ± 2.01 to 38.55 ± 0.72%) and СD8+- suppressors (from 17.38 ± 1.98 to 27.6 ± 0.48%), as well as a trend towards a decrease in СD16+-killers (from 24.55 ± 0.43 to 16.65 ± 0.37%) and an increase in СD19+ В-cells (from 25.12 ± 1.12 to 27.15 ± 0.45%), a decrease of IgE (from 68.3 ± 0.51 to 60.2 ± 0.61%) and CIC (from 54.5 ± 1.22 to 40.3 ± 0.12%). In the control group, there was a trend towards a decrease of СD3+-lymphocytes (59.83 ± 0.7 and 59.27 ± 0.715%) and СD4+-helpers (38.8 ± 0.5 and 38.41 ± 0.64%), normal counts of СD8+-suppressors (28.12 ± 0.58%), and a trend towards a decrease in СD16+-killers (28.2 ± 0.6 and 26.0 ± 0.57%) and an increase in СD19+ В-cells (29.22 ± 0.6 and 29.47 ± 0.58%), a decrease of IgE (68.8 ± 0.91 and 55.2 ± 0.55%) and CIC levels (49.2 ± 0.9 and 45.3 ± 1.05%, respectively). Analysis of cytokine profiles after conventional treatment showed a non-significant decrease in mean levels of allergic inflammatory interleukins (IL) (from 90.5 to 88.3 pg/mL for IL4 and from 68.25 to 67.4 pg/mL for IL5), as well as a non-significant increase in mean concentrations of infectious inflammatory cytokines (from 1.81 to 1.85 pg/mL for IL10 and from 3.72 to 3.89 pg/mL for IFNγ). The use of photohemotherapy led to a decrease in abnormally high IL4 levels almost to the normal (68.55 pg/mL) and to a trend to a decrease in IL5 levels (42.1 pg/mL). Also, there was a significant increase in IL10 (3.91 pg/mL) and IFNγ (5.23 pg/mL) levels to above their baseline values. In patients with severe asthma, a significantly decreased IgM levels were found, both before and after the course of blue light photohemotherapy (0.97 ± 0.1 and 0.89 ± 0.2 g/L, respectively). After treatment, serum IgG levels increased significantly in patients with moderate asthma, compared to baseline (18.3 ± 2.7 and 11.54 ± 0.8 g/L), whereas initially high levels in patients with severe asthma became normal (15.9 ± 4.8 and 18.3 ± 2.7 g/L, respectively).Conclusion: The use of photohemotherapy exerts marked positive effect on blood immunological parameters in asthma patients. The use of blue light is more effective, compared to red light photohemotherapy.
وصف الملف: electronic resource
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6دورية أكاديمية
المؤلفون: V. N. Krasnov, N. R. Paleev
المصدر: Alʹmanah Kliničeskoj Mediciny, Vol 0, Iss 35, Pp 84-88 (2016)
مصطلحات موضوعية: psychosomatics, somatopsychiatry, clinical and pathogenetic inter-relations, bimodal nature of the disorders, integrative medicine, Medicine
الوصف: Integrative medicine represents a promising modern approach to examination and treatment of large population of patients managed by the specialists in psychosomatics and somatopsychiatry. In Russia, experience of teamwork is scarce and non-common. However, implementation of this model of work is encouraged by accumulating evidence of existence of common mechanisms of the development of numerous somatic diseases and psychiatric disorders.
وصف الملف: electronic resource
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7دورية أكاديمية
المؤلفون: F. N. Paleev, N. P. Sanina, A. I. Makarkov, N. N. Khishova, O. V. Moskaletz, N. R. Paleev
المصدر: Российский кардиологический журнал, Vol 0, Iss 1, Pp 32-40 (2016)
مصطلحات موضوعية: infective-immune myocarditis, postmyocarditis cardiosclerosis, lymphocytes, immune activation, Diseases of the circulatory (Cardiovascular) system, RC666-701
الوصف: Aim. To study quantitative parameters, specifics of functional state of the main subpopulations of peripheral blood lymphocytes of the infective-immune myocarditis patients (IIM) and postmyocarditis cardiosclerosis (PMC), and specifics of cytokine profile.Material and methods. Totally, 35 IIM patients included, and 39 with PMC. In 17 patients with IIM there was significant heart failure (HF) — III functional class (FC) by New-York Heart Association (NYHA), in 18 patients with IIM there were no signs of HF, or mild signs (0-II FC by NYHA). In 18 patients with PMC there were no signs of HF, and in 21 — there was I FC by NYHA. The controls were 10 formally healthy persons. Study of population and subpopulation contents, and lymphocytes activation markers of peripheral blood, was done with four-color laser flow cytometry using FACSCalibur equipment and relevant monoclonal antibodies (Beckton Dickinson, USA). We studied the mean cytokines concentrations characterizing Th1-, Th2- and Th17- subpopulations of the helper lymphocytes. Measurement of serum cytokines was done with the method solid-phase immune-enzyme assay with LLC “Vectro-Best” (Russia) media. Statistics was done with software PASW Statistics 18.Results. Inflammatory diseases of myocardium show the deviations of native, as acquired immunity. In IIM and PMC there was significant decrease of NKTlymphocytes, not related to the severity of HF signs and durations of the disease. Immunity activation signs in IIM group showed the increase of the early activator marker CD25 expression activation, that was marked during the first 2 weeks from the disease onset. Following, by the end of the 1st month and on the 2nd month from the disease onset, the increase of T- and non-T-lymphocytes was found with the signs of delayed activation, revealed by HLA-DR antigen expression. The activator marker patterns were differ in patients with different grade of HF severity. Concentration of interferon-γ (IFN-γ) and interleukine (IL)-4 was more than 3 times higher in IIM patients comparing to controls. There was more than 7-times higher increase of IL-17A, and concentrations of effectory cytokines of Th17-subpopulation — IL-8 and granulocyte-macrophagal colony-stimulating factor (GM CSF). The level of IFN-γ reached maximal levels during the first 2 weeks from the disease onset. Later, IFN-γ concentration declined. Opposite, serum level of IL-4 was significantly increased by the end of the 1st and on the 2nd month from disease onset. Concentrations of IL-17A, IL-8 and GM CSF in blood serum were increased during the whole 2nd week, by the end of the 1st month and on the 2nd month. Th17-cytokines concentrations were significantly increased in PMC patients. Level of IL-17A was higher than in controls almost two times, IL8 — by 51%, GM CSF — by 50%. Serum levels of IL-4, and IL-17A, IL-8 and GM CSF were higher in subgroup of PMC patients with the disease duration less than 6 months. Conclusion. Disorders of anti-infection immunity and mechanisms of selfrestriction of immune reactions do play important role in development of myocardium damage of inflammatory origin.
وصف الملف: electronic resource
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8دورية أكاديمية
المؤلفون: N. R. Paleev, P. N. Paleev, E. I. Ostrovsky, V. I. Karandashov, N. P. Sanina, N. N. Khishova, O. V. Moskalets, A. P. Linnik, A. N. Makarkov
المصدر: Кубанский научный медицинский вестник, Vol 0, Iss 1, Pp 191-195 (2014)
مصطلحات موضوعية: миокардит, иммунорегуляция, интерлейкин 17, т-хелперные клетки типа 17, myocarditis, immune regulation, interleukin 17, th17 helper cells, Medicine
الوصف: The review article discusses recent advances in our understanding of viral myocarditis such as pathogenic role of infection and immune mechanisms in acute, sub-acute and chronic phases of the disease. A newly discovered Th17 lymphocyte subset and Interleukin 17 play an important role in the development of inflammatory myocardial diseases. The protective effect of Interferon y and Th1 immune response in acute myocarditis and the role of Th17-mediated mechanisms in progression of acute myocarditis to dilated cardiomyopathy are considered. The evidence of pathogenic role of Th17 lymphocytes in viral myocarditis in human is given.
وصف الملف: electronic resource
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9دورية أكاديمية
المؤلفون: N. R. Paleev, F. N. Paleev
المصدر: Российский кардиологический журнал, Vol 0, Iss 3, Pp 5-9 (2009)
مصطلحات موضوعية: Diseases of the circulatory (Cardiovascular) system, RC666-701
وصف الملف: electronic resource
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10دورية أكاديمية
المؤلفون: N. R. Paleev, E. G. Shuganov, N. A. Raspopina, N. V. Vilchinskaja
المصدر: Кардиоваскулярная терапия и профилактика, Vol 5, Iss 4, Pp 52-56 (2006)
مصطلحات موضوعية: моксинидин, амлодипин, хроническая обструктивная болезнь, гипертоническая болезнь, липиды крови, Diseases of the circulatory (Cardiovascular) system, RC666-701
الوصف: Aim. To study moxonidine and calcium antagonist (amlodipine) effectiveness in essential arterial hypertension (EAH) treatment among patients with chronic obstructive pulmonary disease (COPD), and therapy effects on concomitant dyslipidemia. Material and methods. In total, 30 patients with EAH and Stage 2-3 COPD, aged 39-70 years, were examined. Antihypertensive therapy was started by amlodipine, in the dose of 5 mg/d, increasing up to 10 mg/d 7-10 days later, if target blood pressure (BP) level wasn’t achieved. If target BP figures were not achieved after three-week treatment, moxonidine (0.2-0.4 mg/d) was added. Treatment effectiveness was assessed by spirometry, 24-hour peak flowmetry, 24-hour BP monitoring, lipid profile (LP), glucose and uric acids level assessment. Results. In 16 COPD and EAH patients, BP was normalized in amlodipine monotherapy (5-10 mg/d); in 14 patients – after adding moxonidine (0.4 mg/d). At Week 6 of the combined therapy, LP improved substantially, with blood glucose, uric acid levels and bronchial conductivity remaining the same. Conclusion. To achieve target BP levels in COPD and EAH patients, the combination of amlodipine (10 mg/d) and moxonidine (0.2-0.4 mg/d) can be recommended. It provides effective BP control, as well as improves heart rate variability and BP improvement, without affecting lung function, or blood levels of uric acid and glucose.
وصف الملف: electronic resource
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