المؤلفون: Ssemadaali, Marvin A, Arredondo, Juan, Buser, Elise A, Newmyer, Sherri, Radhakrishnan, Harikrishnan, Javitz, Harold S, Dandekar, Satya, Bhatnagar, Parijat

المصدر: Bioengineering & Translational Medicine. 8(3)

مصطلحات موضوعية: Chemical Engineering, Engineering, Biomedical Engineering, Biotechnology, Emerging Infectious Diseases, Biodefense, Vaccine Related, Lung, Immunization, Infectious Diseases, Prevention, 5.1 Pharmaceuticals, Development of treatments and therapeutic interventions, Inflammatory and immune system, Infection, Good Health and Well Being, cell engineering, cell-based sensors, COVID-19 diagnostics, serology test, synthetic biology, COVID‐19 diagnostics, cell‐based sensors, Biomedical engineering, Chemical engineering

الوصف: We have developed a serology test platform for identifying individuals with prior exposure to specific viral infections and provide data to help reduce public health risks. The serology test composed of a pair of cell lines engineered to express either a viral envelop protein (Target Cell) or a receptor to recognize the Fc region of an antibody (Reporter Cell), that is, Diagnostic-Cell-Complex (DxCell-Complex). The formation of an immune synapse, facilitated by the analyte antibody, resulted into a dual-reporter protein expression by the Reporter Cell. We validated it with human serum with confirmed history of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. No signal amplification steps were necessary. The DxCell-Complex quantitatively detected the target-specific immunoglobulin G (IgG) within 1 h. Validation with clinical human serum containing SARS-CoV-2 IgG antibodies confirmed 97.04% sensitivity and 93.33% specificity. The platform can be redirected against other antibodies. Self-replication and activation-induced cell signaling, two attributes of the cell, will enable rapid and cost-effective manufacturing and its operation in healthcare facilities without requiring time-consuming signal amplification steps.

وصف الملف: application/pdf

URL الوصول: https://escholarship.org/uc/item/33j6x77x

المؤلفون: Ssemadaali, Marvin A, Newmyer, Sherri, Radhakrishnan, Harikrishnan, Arredondo, Juan, Javitz, Harold S, Dandekar, Satya, Bhatnagar, Parijat

المصدر: Microbiology Spectrum. 10(4)

مصطلحات موضوعية: Microbiology, Biological Sciences, Biodefense, Infectious Diseases, Prevention, Lung, Pneumonia & Influenza, Vaccine Related, Emerging Infectious Diseases, Infection, Good Health and Well Being, Animals, COVID-19, COVID-19 Testing, Mice, Pandemics, SARS-CoV-2, Specimen Handling, cell-based sensors, COVID-19 diagnostics, antigen test, reporter cell, CAR T-cell

الوصف: We have engineered a cell that can be used for diagnosing active severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections. Isolation of individuals with active infections offers an effective solution for mitigating pandemics. However, the implementation of this practice requires robust infrastructure for rapid and intuitive testing, which is currently missing in our communities. To address this need, we engineered a fast-growing cell line into a cell-based antigen test platform for emerging viruses, i.e., DxCell, that can be rapidly deployed in decentralized health care facilities for continuous testing. The technology was characterized using cells engineered to present spike glycoprotein of SARS-CoV-2 (SARS-CoV-2-Sgp-cells) and Calu-3 host cells infected with competent SARS-CoV-2. Preclinical validation was conducted by directly incubating the DxCell with oropharyngeal swabs from mice infected with SARS-CoV-2. No sample preparation steps are necessary. The DxCell quantitatively detected the SARS-CoV-2-Sgp-cells within 1 h (P

وصف الملف: application/pdf

URL الوصول: https://escholarship.org/uc/item/8t81c742

المؤلفون: Osborn, Harmony, Newmyer, Aileen, Krebs, William

المصدر: In Air Medical Journal July-August 2024 43(4):345-347

المؤلفون: Newmyer, Lauren^{Aff1, Aff2, IDs11113023098003_cor1}, Frisco, Michelle L.

المصدر: Population Research and Policy Review: in cooperation with the Southern Demographic Association (SDA). 42(4)

المؤلفون: Harikrishnan Radhakrishnan, Sherri L. Newmyer, Marvin A. Ssemadaali, Harold S. Javitz, Parijat Bhatnagar

المصدر: Bioengineering & Translational Medicine, Vol 9, Iss 1, Pp n/a-n/a (2024)

مصطلحات موضوعية: CAR T cells, cell engineering, cell manufacturing, cell‐based drug delivery, synthetic biology, Chemical engineering, TP155-156, Biotechnology, TP248.13-248.65, Therapeutics. Pharmacology, RM1-950

الوصف: Abstract Primary T cell has been transformed into a cell‐based delivery platform that synthesizes complex biologics at the disease site with spatiotemporal resolution. This broadly applicable technology can circumvent toxicities due to systemic administration of biologics that necessitates the use of high doses and may diffuse to the healthy tissues. Its clinical translation, however, has been impeded by manufacturing bottlenecks. In this work, a range of process parameters were investigated for increasing the production yield of the primary T cells engineered for delivery function. Compared to the common spinoculation‐based method, the transduction yield was enhanced ~2.5‐fold by restricting the transduction reaction volume for maximizing the lentivector‐to‐T‐cell contact. Cell density and cytokines used in the expansion process were adjusted to achieve >100‐fold expansion of the T‐cell‐based delivery platform in 14 days, and the function of these cells was validated in vivo using intraperitoneally implanted tumor cells. The primary T‐cell‐based delivery platform has human applications because it can be scaled and administrated to express a broad range of therapeutic proteins (e.g., cytokines, interferons, enzymes, agonists, and antagonists) at the disease site, obviating the need for systemic delivery of large doses of these proteins.

وصف الملف: electronic resource

Relation: https://doaj.org/toc/2380-6761

URL الوصول: https://doaj.org/article/acfca9628e7d49fab2fdd5f789b3efe8

المؤلفون: Newmyer, Lauren, Evans, Megan, Graif, Corina

المصدر: Demography, 2022 Aug 01. 59(4), 1299-1323.

URL الوصول: https://www.jstor.org/stable/48681690

المؤلفون: Krebs, William, Newmyer, Aileen, Dzurik, Alexander, Burgard, Kristine, Scaff, Tyler, Waltmire, Jason, Wilson, Todd, Kimmett, Cora, Stausmire, Julie, Buderer, Nancy

المصدر: In Air Medical Journal January-February 2024 43(1):19-22

المؤلفون: Marvin A. Ssemadaali, Juan Arredondo, Elise A. Buser, Sherri Newmyer, Harikrishnan Radhakrishnan, Harold S. Javitz, Satya Dandekar, Parijat Bhatnagar

المصدر: Bioengineering & Translational Medicine, Vol 8, Iss 3, Pp n/a-n/a (2023)

مصطلحات موضوعية: cell engineering, cell‐based sensors, COVID‐19 diagnostics, serology test, synthetic biology, Chemical engineering, TP155-156, Biotechnology, TP248.13-248.65, Therapeutics. Pharmacology, RM1-950

الوصف: Abstract We have developed a serology test platform for identifying individuals with prior exposure to specific viral infections and provide data to help reduce public health risks. The serology test composed of a pair of cell lines engineered to express either a viral envelop protein (Target Cell) or a receptor to recognize the Fc region of an antibody (Reporter Cell), that is, Diagnostic‐Cell‐Complex (DxCell‐Complex). The formation of an immune synapse, facilitated by the analyte antibody, resulted into a dual‐reporter protein expression by the Reporter Cell. We validated it with human serum with confirmed history of severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) infection. No signal amplification steps were necessary. The DxCell‐Complex quantitatively detected the target‐specific immunoglobulin G (IgG) within 1 h. Validation with clinical human serum containing SARS‐CoV‐2 IgG antibodies confirmed 97.04% sensitivity and 93.33% specificity. The platform can be redirected against other antibodies. Self‐replication and activation‐induced cell signaling, two attributes of the cell, will enable rapid and cost‐effective manufacturing and its operation in healthcare facilities without requiring time‐consuming signal amplification steps.

وصف الملف: electronic resource

Relation: https://doaj.org/toc/2380-6761

URL الوصول: https://doaj.org/article/4df3d1b712484c9d87009d19de5fa068

المؤلفون: Bai, Liu^Aff7, Newmyer, Lauren^Aff7

المساهمون: McHale, Susan M., Series Editor^{Aff1, Aff6}, King, Valarie, Series Editor^{Aff2, Aff5}, Glick, Jennifer E., Series Editor^{Aff3, Aff4}

المصدر: Parent-Child Separation : Causes, Consequences, and Pathways to Resilience. 1:231-240

المؤلفون: Newmyer, Lauren, Yabiku, Scott T.

المصدر: In Social Science Research November 2022 108