المؤلفون: Patil, Rajkumar V.^{Aff1, IDs41870020005309_cor1}, Mahalle, Parikshit N., Shinde, Gitanjali R.

المصدر: International Journal of Information Technology: An Official Journal of Bharati Vidyapeeth's Institute of Computer Applications and Management. 14(3):1355-1365

المؤلفون: Patil, Rajkumar V.^Aff6, Mahalle, Parikshit N.^Aff6, Shinde, Gitanjali R.^Aff6

المساهمون: Kacprzyk, Janusz, Series Editor^Aff1, Mahalle, Parikshit N., editor^Aff2, Shinde, Gitanjali R., editor^Aff3, Dey, Nilanjan, editor^Aff4, Hassanien, Aboul Ella, editor^Aff5

المصدر: Security Issues and Privacy Threats in Smart Ubiquitous Computing. 341:195-211

لا يتم عرض هذه النتيجة على الضيوف.
تسجيل الدخول للوصول الكامل.

المؤلفون: Poomathi, Nataraj, Singh, Sunpreet, Prakash, Chander, Patil, Rajkumar V., Perumal, P.T., Barathi, Veluchamy Amutha, Balasubramanian, Kalpattu K., Ramakrishna, Seeram, Maheshwari, N.U.

المصدر: Rapid Prototyping Journal, 2018, Vol. 25, Issue 3, pp. 496-514.

URL الوصول: http://www.emeraldinsight.com/doi/10.1108/RPJ-06-2018-0144

المؤلفون: Agre, Peter, Lee, M. Douglas, Devidas, Sreenivas, Guggino, William B., Sasaki, Sei, Uchida, Shinichi, Kuwahara, Michio, Fushimi, Kiyohide, Marumo, Fumiaki, Verkman, A. S., Yang, Baoxue, Deen, P. M. T., Mulders, S. M., Kansen, S. M., van Os, C. H., Fischbarg, Jorge, Kuang, Kunyan, Li, Jun, Iserovich, Pavel, Wen, Qian, Patil, Rajkumar V., Han, Zhiquang, Wax, Martin B., Regan, John W., Stamer, W. Daniel, Yool, Andrea J.

المصدر: Science, 1997 Mar 01. 275(5305), 1490-1492.

URL الوصول: https://www.jstor.org/stable/2892284

المؤلفون: Han, Zhiqiang^Aff3, Patil, Rajkumar V.^{Aff3, Aff4}

المساهمون: Hohmann, Stefan, editor^Aff1, Nielsen, Søren, editor^Aff2

المصدر: Molecular Biology and Physiology of Water and Solute Transport. :107-113

المؤلفون: Datta, Prasanta, Goss, Thomas J., Omnaas, John R., Patil, Rajkumar V.

المصدر: Proceedings of the National Academy of Sciences of the United States of America, 1987 Jan . 84(2), 393-397.

URL الوصول: https://www.jstor.org/stable/29018

المؤلفون: Shankardas, Jwalitha, Patil, Rajkumar V., Vishwanatha, Jamboor K.

المصدر: Molecular Vision

مصطلحات موضوعية: Aquaporin 1, Down-Regulation, Endothelial Cells, Epithelial Cells, Immunohistochemistry, Aquaporin 5, Cell Line, Cornea, Protein Transport, Cell Movement, Humans, sense organs, Gene Silencing, Phosphorylation, RNA, Small Interfering, Extracellular Signal-Regulated MAP Kinases, Research Article, Cell Proliferation, Subcellular Fractions

الوصف: Purpose The purpose of this study was to determine the effects of down-regulation of Aquaporin 1 (AQP1) and Aquaporin 5 (AQP5) on cell proliferation and migration in human corneal endothelial (HCEC) and human corneal epithelial (CEPI17) cell lines, respectively. Methods AQP1 and AQP5 were down regulated using siRNA following lipofectamine-mediated transfection in corneal endothelial and epithelial cells, respectively. Down-regulation was confirmed using RT–PCR, indirect immunofluorescence, and immunoblot analysis. Total internal reflection fluorescence (TIRF) microscopy was used to detect cell surface aquaporin expression. Cell proliferation was determined by SRB (sulfrodamine B) assay. Cell migration was determined by in vitro wound healing and migration assay. Results In HCEC cells, AQP1 was localized to the cytosol as well as cell membrane and its down-regulation resulted in decreased cell proliferation and migration with a significant decrease in phosphorylated ERK (pERK). In CEPI17 cells AQP5 protein expression was also localized to cytosol as well as cell membrane. AQP5 down-regulation resulted in an increase in proliferation and cell migration with no significant difference in pERK. Conclusions AQP1 plays a role in HCEC proliferation and migration via the ERK signaling pathway and therefore may have significant implications in corneal endothelial dysfunction whereas; AQP5 may play an indirect role in human corneal epithelial cell proliferation and migration.

URL الوصول: https://explore.openaire.eu/search/publication?articleId=pmid________::2c6b594fd98e9ce3e03c64db3145b30b
http://europepmc.org/articles/PMC2925904

لا يتم عرض هذه النتيجة على الضيوف.
تسجيل الدخول للوصول الكامل.

لا يتم عرض هذه النتيجة على الضيوف.
تسجيل الدخول للوصول الكامل.