المؤلفون: Kiasat, Ali, Prast-Nielsen, Stefanie, Rautiainen, Susanne, Engstrand, Lars, Andersson, Fredrik, Lindberg, Johan, Schuppe-Koistinen, Ina, Löf Granström, Anna, Gustafsson, Ulf O.

المصدر: Scandinavian Journal of Gastroenterology.

الوصف: Background: In addition to facilitating lipid digestions, bile acids (BA) are signalling molecules acting on receptors on immune cells and along the gastrointestinal (GI) tract. The aim of this study was to assess if altered bile acid profiles in plasma are associated with Crohn’s disease (CD). Method: This cross-sectional study included individuals (aged ≥18 years) referred for colonoscopy at a tertiary centre in Stockholm between 2016 and 2019. All participants received bowel preparation, completed a lifestyle questionnaire and provided blood samples for analysis. During colonoscopy, severity of disease was graded, and biopsies were taken from colonic mucosa. In the current substudy, 88 individuals with CD and 88 age-matched controls were selected for analysis of BA in plasma with ultra performance liquid chromatography (UPLC). Linear regression models were then used to compare mean bile acid concentrations and concentration ratios between CD and controls. Results: Individuals with CD had lower plasma concentrations of the majority of secondary BA compared to controls, in total CD/CC ratio 0.60 (SE 0.12), p = 0.001. The most prominent observations were lower levels of deoxycolic acid derivates and lithocolic acid derivates among participants with CD. Moreover, plasma concentration for secondary BA among participants with active CD was significantly lower compared to those with CD in remission, CD active/CD remission ratio 0.65 (SE 0.11), p < 0.002. Conclusion: Crohn’s disease may be associated with altered plasma bile acid composition. The significance of colonic bacterial diversity in this context needs to be investigated in further studies.

وصف الملف: electronic

URL الوصول: https://urn.kb.se/resolve?urn=urn:nbn:se:ri:diva-72901
https://doi.org/10.1080/00365521.2024.2328592
https://ri.diva-portal.org/smash/get/diva2:1854685/FULLTEXT01.pdf

المؤلفون: Nyström, Niklas, Prast-Nielsen, Stefanie, Correia, Mario, Globisch, Daniel, Engstrand, Lars, Schuppe-Koistinen, Ina, Halfvarson, Jonas

المصدر: Journal of Crohn's & Colitis. 17(3):418-432

مصطلحات موضوعية: Inflammatory bowel disease, metabolome, paediatric

الوصف: Background and AimsTo advance the understanding of inflammatory bowel disease [IBD] pathophysiology, we compared the mucosal and plasma metabolomes between new-onset paediatric IBD patients and symptomatic non-IBD controls, and correlated plasma inflammation markers and disease characteristics with the altered metabolites.MethodsPaired colonic and ileal biopsies and plasma from 67 treatment-naïve children with incident Crohn’s disease [CD; n = 47], ulcerative colitis [UC; n = 9], and non-IBD controls [n = 11] were analysed using ultra-performance liquid chromatography-mass spectrometry [UPLC-MS/MS]. Inflammatory plasma proteins [n = 92] were assessed.ResultsThe metabolomes in inflamed mucosal biopsies differed between IBD patients and controls. In CD, mucosal levels of several lysophospholipids [lysophosphatidylcholines, lysophosphatidyletanolamines, lysophosphatidylinositols, and lysophosphatidylserines] were decreased, correlating with various plasma metabolites including amino acid analogues and N-acetylated compounds. In both CD and UC, mucosal sphingolipids, including ceramide [d18:2/24:1, d18:1/24:2], lactosyl-N-palmitoyl-sphingosine [d18:1/16:0], behenoyl sphingomyelin [d18:1/22:0], lignoceroyl sphingomyelin [d18:1/24:0], and/or sphingomyelin [d18:1/24:1, d18:2/24:0] were increased, correlating with sphingolipids, bile acids, and/or N-acetylated metabolites in plasma. Among proteins associated with CD, interleukin-24 correlated with plasma metabolites, including lactosyl-N-palmitoyl sphingosine [d18:1/16:0] and phosphatidyletanolamine [18:1/18:1], haemoglobin, and faecal calprotectin. In UC, interleukin-24, interleukin-17A, and C-C motif chemokine 11 correlated with several plasma metabolites, including N-acetyltryptophan, tryptophan, glycerate, and threonate, and with the Paediatric Ulcerative Colitis Activity Index, C-reactive protein, and faecal calprotectin.ConclusionsMucosal perturbations of lysophospholipids and sphingolipids characterised the metabolome in new-onset paediatric IBD and correlated with plasma metabolites. By integrating plasma metabolomics data with inflammatory proteins and clinical data, we identified clinical and inflammatory markers associated with metabolomic signatures for IBD.

وصف الملف: electronic

URL الوصول: https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-524107
https://doi.org/10.1093/ecco-jcc/jjac149
https://uu.diva-portal.org/smash/get/diva2:1841450/FULLTEXT01.pdf

المؤلفون: Prast-Nielsen, Stefanie

مصطلحات موضوعية: Thioredoxin-Disulfide Reductase -- physiology, Apoptosis, Cell Survival, Cell Line, Tumor, Antineoplastic Agents -- antagonists & inhibitors, Antineoplastic Agents -- pharmacology, Antineoplastic Agents -- therapeutic use, Selenium -- physiology, Selenocysteine -- physiology

URL الوصول: http://diss.kib.ki.se/2009/978-91-7409-576-0/

Degree: Diss. (sammanfattning) Stockholm : Karolinska institutet, 2009

المؤلفون: Stiernborg, Miranda, Prast-Nielsen, Stefanie, Melas, Philippe A., Skott, Maria, Millischer, Vincent, Boulund, Fredrik, Forsell, Yvonne, Lavebratt, Catharina

المصدر: In Brain Behavior and Immunity March 2024 117:298-309

المؤلفون: Oláh, Peter, Szlávicz, Eszter, Kuchner, Marcus, Nemmer, Jana, Zeeuwen, Patrick, Lefèvre-Utile, Alain, Fyhrquist, Nanna, Prast-Nielsen, Stefanie, Skoog, Tiina, Serra, Angela, Rodríguez, Elke, Raap, Ulrike, Meller, Stephan, Gyulai, Rolland, Hupé, Philippe, Kere, Juha, Levi-Schaffer, Francesca, Tsoka, Sophia, Alexander, Helen, Nestle, Frank O., Schröder, Jens M., Weidinger, Stephan, van den Bogaard, Ellen, Soumelis, Vassili, Greco, Dario, Barker, Jonathan, Lauerma, Antti, Ranki, Annamari, Andersson, Björn, Alenius, Harri, Homey, Bernhard

المصدر: In Journal of Dermatological Science June 2022 106(3):132-140

المؤلفون: Shi, Wenpei, Hu, Yi, Ning, Zhu, Xia, Fan, Wu, Meiying, Hu, Yue O.O., Chen, Cheng, Prast-Nielsen, Stefanie, Xu, Biao

المصدر: In International Journal of Infectious Diseases October 2021 111:313-321

المؤلفون: Dahlin, Maria, Prast-Nielsen, Stefanie

المصدر: In eBioMedicine June 2019 44:741-746

المؤلفون: Kiasat, Ali, Rautiainen, Susanne, Prast-Nielsen, Stefanie, Engstrand, Lars, Schuppe-Koistinen, Ina, Gustafsson, Ulf O., Löf Granström, Anna

المصدر: Scandinavian Journal of Gastroenterology; Nov2023, Vol. 58 Issue 11, p1246-1252, 7p

مصطلحات موضوعية: SHORT-chain fatty acids, INFLAMMATORY bowel diseases, CROHN'S disease, FATTY acid analysis, ULCERATIVE colitis

مستخلص: Specific variations of short chain fatty acids in fecal samples have been shown for patients with inflammatory bowel disease. The aim of this study was to assess if Crohn's disease and ulcerative colitis are associated with altered concentrations of short chain fatty acids also in blood plasma. Between 2016-2019, Swedish adults referred to a tertiary center for colonoscopy were asked to participate in a cross-sectional study. Individuals with Crohn's disease or ulcerative colitis as well as individuals with no findings on the colonoscopy (defined as clean colon) were included in the study. Data on colonoscopy findings, blood samples (including haemoglobin, C-reactive protein and short chain fatty acid analysis) as well as a validated lifestyle questionnaire including 277 questions were collected from all participants. Linear regression was used to compare mean concentrations of short chain fatty acids between Crohn's disease, ulcerative colitis and clean colon. The cohort consisted of 132 individuals with Crohn's disease, 119 with ulcerative colitis and 205 with clean colon. In the crude model, succinic acid was significantly lower (p < 0.05) among patients with Crohn's disease (mean 3.00 µM SE 0.10) and ulcerative colitis (mean 3.13 µM SE 0.10) in comparison to clean colon (mean 3.41 µM SE 0.08), however when adjusting for sex, age and diet the results did not remain statistically significant. No differences in plasma concentration of the other measured short chain fatty acids were detected. Crohn's disease and ulcerative colitis are not associated with altered short chain fatty acid concentrations in plasma. Further research is needed to confirm or refute our findings. In this cross-sectional study including individuals with inflammatory bowel disease and healthy subjects we found no association between Crohn's disease and ulcerative colitis and short chain fatty acid concentrations in plasma. [ABSTRACT FROM AUTHOR]

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المؤلفون: Fyhrquist, Nanna^{Aff1, Aff2}, Muirhead, Gareth^{Aff3, Aff4}, Prast-Nielsen, Stefanie, Jeanmougin, Marine^{Aff6, Aff7, Aff8, Aff9}, Olah, Peter^{Aff10, Aff11}, Skoog, Tiina, Jules-Clement, Gerome^{Aff6, Aff7, Aff8, Aff9}, Feld, Micha, Barrientos-Somarribas, Mauricio, Sinkko, Hanna^{Aff1, Aff2}, van den Bogaard, Ellen H., Zeeuwen, Patrick L.J.M., Rikken, Gijs, Schalkwijk, Joost, Niehues, Hanna, Däubener, Walter, Eller, Silvia Kathrin, Alexander, Helen, Pennino, Davide, Suomela, Sari, Tessas, Ioannis, Lybeck, Emilia, Baran, Anna M., Darban, Hamid, Gangwar, Roopesh Singh, Gerstel, Ulrich, Jahn, Katharina, Karisola, Piia, Yan, Lee, Hansmann, Britta, Katayama, Shintaro, Meller, Stephan, Bylesjö, Max, Hupé, Philippe^{Aff6, Aff7, Aff8, Aff21}, Levi-Schaffer, Francesca, Greco, Dario^{Aff22, Aff23, Aff24}, Ranki, Annamari, Schröder, Jens M., Barker, Jonathan, Kere, Juha^{Aff12, Aff25}, Tsoka, Sophia, Lauerma, Antti, Soumelis, Vassili^{Aff6, Aff9}, Nestle, Frank O., Homey, Bernhard, Andersson, Björn, Alenius, Harri^{Aff1, Aff2, IDs4146701912253y_cor47}

المصدر: Nature Communications. 10(1)

المؤلفون: Lindefeldt, Marie, Eng, Alexander, Darban, Hamid, Bjerkner, Annelie, Zetterström, Cecilia K, Allander, Tobias, Andersson, Björn, Borenstein, Elhanan^{Aff2, Aff6, Aff7, Aff8, Aff9}, Dahlin, Maria, Prast-Nielsen, Stefanie^Aff10

المصدر: npj Biofilms and Microbiomes. 5(1)