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1دورية أكاديمية
المؤلفون: Satya Ranjan Sahu, Abinash Dutta, Doureradjou Peroumal, Premlata Kumari, Bhabasha Gyanadeep Utakalaja, Shraddheya Kumar Patel, Narottam Acharya
المصدر: EMBO Molecular Medicine, Vol 16, Iss 6, Pp 1254-1283 (2024)
مصطلحات موضوعية: Antifungal Vaccine, Cytokines, Neutrophils, Dectin-1, Trained Immunity, Medicine (General), R5-920, Genetics, QH426-470
الوصف: Abstract Disseminated fungal infections account for ~1.5 million deaths per year worldwide, and mortality may increase further due to a rise in the number of immunocompromised individuals and drug-resistance fungal species. Since an approved antifungal vaccine is yet to be available, this study explored the immunogenicity and vaccine efficacy of a DNA polymerase mutant strain of Candida albicans. CNA25 is a pol32ΔΔ strain that exhibits growth defects and does not cause systemic candidiasis in mice. Immunized mice with live CNA25 were fully protected against C. albicans and C. parapsilosis but partially against C. tropicalis and C. glabrata infections. CNA25 induced steady expression of TLR2 and Dectin-1 receptors leading to a faster recognition and clearance by the immune system associated with the activation of protective immune responses mostly mediated by neutrophils, macrophages, NK cells, B cells, and CD4+ and CD8+ T cells. Molecular blockade of Dectin-1, IL-17, IFNγ, and TNFα abolished resistance to reinfection. Altogether, this study suggested that CNA25 collectively activates innate, adaptive, and trained immunity to be a promising live whole-cell vaccine against systemic candidiasis.
وصف الملف: electronic resource
Relation: https://doaj.org/toc/1757-4684
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2دورية أكاديمية
المؤلفون: Satya Ranjan Sahu, Shweta Thakur, Doureradjou Peroumal, Bhabasha Gyanadeep Utkalaja, Abinash Dutta, Premlata Kumari, Ipsita Subhadarsini, Narottam Acharya
المصدر: Frontiers in Immunology, Vol 14 (2023)
مصطلحات موضوعية: DNA damage response, carcinogenesis, cell death, p53, oral squamous cell carcinoma, cancer immunology, Immunologic diseases. Allergy, RC581-607
الوصف: 4-Nitroquinoline N-oxide (4-NQO) and its derivatives react with genomic DNA to form stable quinolone monoadducts, which are highly mutagenic and genotoxic. While the chronic high-dose exposure of epithelial cells to a carcinogen such as 4-NQO leads to tumor development, its effect on other cells has not been explored yet. Since the immunosuppression due to aberrant immunological profile is recognized as a significant cause in tumors, here we determine the interaction between 4-NQO and immune cells both in vivo and in vitro, and its effect on oral squamous cell carcinoma (OSCC) progression in a murine model. Immune cell profiling of the spleen and peripheral blood revealed a significant decrease in the B-cell population in 4-NQO-exposed mice than the untreated group. Additionally, γδ T and CD5+ B lymphocyte populations decreased at both pre- and post-cancerous stages of OSCC. These results suggested that 4-NQO induced tumor transition from pre-malignant lesions to OSCC by altering certain immune cells systemically. Next, to establish the effect of 4-NQO on immune cells, human B- and T-cell lines were subjected to 4-NQO; the reduction in cell viability, increase in DNA damage response marker, and induction of apoptosis were more pronounced in B than T cells. Altogether, our results indicated that in addition to the genotoxicity of oral epithelial cells, 4-NQO potentiates long-range effects on specific immune cells to induce cell death to cause very-early immunosuppressive response during oral carcinogenesis, and thus immunosuppression and tumor development are coevolved.
وصف الملف: electronic resource
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3دورية أكاديمية
المؤلفون: Doureradjou Peroumal, Satya Ranjan Sahu, Premlata Kumari, Bhabasha Gyanadeep Utkalaja, Narottam Acharya
المصدر: Microbiology Spectrum, Vol 10, Iss 5 (2022)
مصطلحات موضوعية: microbiome, mycobiome, diet, genomics, BMI, hormone, Microbiology, QR1-502
الوصف: ABSTRACT Candida albicans survives as a commensal fungus in the gastrointestinal tract, and that its excessive growth causes infections in immunosuppressed individuals is widely accepted. However, any mutualistic relationship that may exist between C. albicans and the host remains undetermined. Here, we showed that a long-term feeding of C. albicans does not cause any noticeable infections in the mouse model. Our 16S and 18S ribosomal DNA (rDNA) sequence analyses suggested that C. albicans colonizes in the gut and modulates microbiome dynamics, which in turn mitigates high-fat-diet-induced uncontrolled body weight gain and metabolic hormonal imbalances. Interestingly, adding C. albicans to a nonobesogenic diet stimulated the appetite-regulated hormones and helped the mice maintain a healthy body weight. In concert, our results suggest a mutualism between C. albicans and the host, contrary to the notion that C. albicans is always an adversary and indicating it can instead be a bona fide admirable companion of the host. Finally, we discuss its potential translational implication as a probiotic, especially in obese people or people dependent on high-fat calorie intakes to manage obesity associated complications. IMPORTANCE Candida albicans is mostly considered an opportunistic pathogen that causes fetal systemic infections. However, this study demonstrates that in its commensal state, it maintains a long-term mutualistic relationship with the host and regulates microbial dynamics in the gut and host physiology. Thus, we concluded that C. albicans is not always an adversary but rather can be a bona fide admirable companion of the host. More importantly, as several genomic knockout strains of C. albicans were shown to be avirulent, such candidate strains may be explored further as preferable probiotic isolates to control obesity.
وصف الملف: electronic resource
Relation: https://doaj.org/toc/2165-0497
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4دورية أكاديمية
المؤلفون: Satya Ranjan Sahu, Swagata Bose, Manish Singh, Premlata Kumari, Abinash Dutta, Bhabasha Gyanadeep Utkalaja, Shraddheya Kumar Patel, Narottam Acharya
المصدر: Frontiers in Cellular and Infection Microbiology, Vol 12 (2022)
مصطلحات موضوعية: Mycosis, Candida, drug resistance, immunity, whole cell vaccine, pan-fungal vaccine, Microbiology, QR1-502
الوصف: Candidiasis is a mycosis caused by opportunistic Candida species. The occurrence of fungal infections has considerably increased in the last few years primarily due to an increase in the number of immune-suppressed individuals. Alarming bloodstream infections due to Candida sp. are associated with a higher rate of morbidity and mortality, and are emerged as major healthcare concerns worldwide. Currently, chemotherapy is the sole available option for combating fungal diseases. Moreover, the emergence of resistance to these limited available anti-fungal drugs has further accentuated the concern and highlighted the need for early detection of fungal infections, identification of novel antifungal drug targets, and development of effective therapeutics and prophylactics. Thus, there is an increasing interest in developing safe and potent immune-based therapeutics to tackle fungal diseases. In this context, vaccine design and its development have a priority. Nonetheless, despite significant advances in immune and vaccine biology over time, a viable commercialized vaccine remains awaited against fungal infections. In this minireview, we enumerate various concerted efforts made till date towards the development of anti-Candida vaccines, an option with pan-fugal vaccine, vaccines in the clinical trial, challenges, and future opportunities.
وصف الملف: electronic resource
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5دورية أكاديمية
المصدر: Pharmaceutical Sciences, Vol 27, Iss 2, Pp 238-250 (2021)
مصطلحات موضوعية: garcinia, extracts, aunps, antioxidant, cytotoxicity, Pharmacy and materia medica, RS1-441
الوصف: Background: Green synthesis of gold nanoparticles (AuNPs) using medicinal plant extract is an emerging area of research due to their applicability in nanomedicines. Methods: In this study, aqueous extracts prepared from fruit-pericarps of two Garcinia species, G. indica (GI) and G. cambogia (GC) fruits which are important medicinally and commercially have been utilized for the synthesis of AuNPs. Various analytical techniques were utilized to characterize the synthesized AuNPs. The synthesized AuNPs were investigated for their biological properties such as antioxidant activity using the (2,2-diphenyl-1-picrylhydrazyl) DPPH model, cytotoxicity against MCF-7 (breast) cancer cell line, and antibacterial activity against two bacterial strains viz. B. subtilis and E. coli. Results: The absorption peak of the AuNPs is observed at 541 nm using UV–Visible spectroscopy. The high resolution – scanning electron microscopy images showed spherical with a triangular shape AuNPs and their average sizes were ranging from 2 – 10 nm and it was found to be in good agreement with the particle size of 8 – 11 nm determined using X-ray diffraction analysis. Fourier-transform infrared spectroscopy revealed that water-soluble biomolecules from the aqueous extracts of the Garcinia species played a crucial role in the formation of AuNPs. The synthesized AuNPs exhibited considerable cytotoxicity with IC50 values 34.55 µg/ml (GI) and 35.69 µg/ml (GC) against the MCF-7 cancer cell line. Furthermore, synthesized AuNPs also demonstrated significant antioxidant and antibacterial properties comparable to the standards used. Conclusion: AuNPs have been synthesized using a simple green approach. The synthesized AuNPs demonstrated promising cytotoxicity, antioxidant, and antibacterial properties.
وصف الملف: electronic resource
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6دورية أكاديمية
المؤلفون: Divyesh Patel, Premlata Kumari, Navin B. Patel
المصدر: Arabian Journal of Chemistry, Vol 10, Iss S2, Pp S3990-S4001 (2017)
مصطلحات موضوعية: Coumarin, Chalcones, Antibacterial activity, Antifungal activity, Antimycobacterial activity, Chemistry, QD1-999
الوصف: The titled compounds (6′a–k), (7′a–k) and (8′a–k) were synthesized from chalcones (5a–k) having coumarin moiety. Cyclization of chalcones (5a–k) with hydroxyl amine hydrochloride, thiourea and urea resulted in corresponding isoxazoles (6′a–k), pyrimidinthiones (7′a–k) and pyrimidin-2-ones (8′a–k). Structures of newly synthesized compounds were established on the basis of their elemental analysis, IR, 1H NMR, 13C NMR, and mass spectral data. The synthesized analogs were evaluated for their antimycobacterial activity and antimicrobial activity against eight bacteria (Staphylococcus aureus, Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Salmonella typhi, Proteus vulgaris, and Shigella flexneri) and four fungi (Aspergillus niger, Candida albicans, Aspergillus fumigatus, and Aspergillus clavatus).
وصف الملف: electronic resource
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7دورية أكاديمية
المؤلفون: Mithlesh Rajput, Meenakshi Dahiya, Premlata Kumari, Kamini Kalra, Manjeet Aggarwal, R. K. Khandal
المصدر: E-Journal of Chemistry, Vol 8, Iss 4, Pp 1770-1783 (2011)
الوصف: Voglibose is a potent α glucosidase inhibitor, used for the treatment of diabetes mellitus. For quantitative determination of voglibose in pharmaceutical formulations of low doses, simple, sensitive, accurate and precise LC-MS/MS method using electrospray ionization in positive mode was developed and validated. The method was found linear in the concentration range of 25.0-1200 ηg/mL with a correlation coefficient of 0.9998. The limit of detection (LOD) of the method was found to be 1.5 ηg/mL and limit of quantitation (LOQ) was achieved at 3.0 ηg/mL. The recoveries of voglibose from spiked samples at different concentration levels were found in the range of 98-102%. The proposed method was found suitable for quantitation of voglibose and for the determination of uniformity of content of the dosage units of the tablet formulations.
وصف الملف: electronic resource
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8
المؤلفون: Ajayrajsinh R. Zala, Dinesh Kumar, Uvais Razakhan, Dhanji P. Rajani, Iqrar Ahmad, Harun Patel, Premlata Kumari
المصدر: Journal of Biomolecular Structure and Dynamics. :1-12
مصطلحات موضوعية: Structural Biology, General Medicine, Molecular Biology
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9
المؤلفون: Premlata Kumari, Azazahemad A. Kureshi
المصدر: Photoreactors in Advanced Oxidation Processes. :71-108
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_________::b83a54c448c4baa6e1445d510bfdd657
https://doi.org/10.1002/9781394167289.ch3 -
10
المؤلفون: Ajayrajsinh R. Zala, Dhanji P. Rajani, Premlata Kumari
المصدر: Journal of the Iranian Chemical Society.
مصطلحات موضوعية: General Chemistry
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_________::0c68aaafb1d19afcc70e1f63b566458e
https://doi.org/10.1007/s13738-023-02801-1
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