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1دورية أكاديمية
المؤلفون: Robert M. Tighe, Anastasiya Birukova, Yuryi Malakhau, Yoshihiko Kobayashi, Aaron T. Vose, Vidya Chandramohan, Jaime M. Cyphert-Daly, R. Ian Cumming, Helene Fradin Kirshner, Purushothama R. Tata, Jennifer L. Ingram, Michael D. Gunn, Loretta G. Que, Yen-Rei A. Yu
المصدر: Frontiers in Immunology, Vol 15 (2024)
مصطلحات موضوعية: macrophages, asthma, ontogeny, single-cell RNA-seq, house dust mite, Immunologic diseases. Allergy, RC581-607
الوصف: IntroductionEnvironmental exposures and experimental manipulations can alter the ontogenetic composition of tissue-resident macrophages. However, the impact of these alterations on subsequent immune responses, particularly in allergic airway diseases, remains poorly understood. This study aims to elucidate the significance of modified macrophage ontogeny resulting from environmental exposures on allergic airway responses to house dust mite (HDM) allergen.MethodsWe utilized embryonic lineage labeling to delineate the ontogenetic profile of tissue-resident macrophages at baseline and following the resolution of repeated lipopolysaccharide (LPS)-induced lung injury. We investigated differences in house dust mite (HDM)-induced allergy to assess the influence of macrophage ontogeny on allergic airway responses. Additionally, we employed single-cell RNA sequencing (scRNAseq) and immunofluorescent staining to characterize the pulmonary macrophage composition, associated pathways, and tissue localization.ResultsOur findings demonstrate that the ontogeny of homeostatic alveolar and interstitial macrophages is altered after the resolution from repeated LPS-induced lung injury, leading to the replacement of embryonic-derived by bone marrow-derived macrophages. This shift in macrophage ontogeny is associated with reduced HDM-induced allergic airway responses. Through scRNAseq and immunofluorescent staining, we identified a distinct subset of resident-derived interstitial macrophages expressing genes associated with allergic airway diseases, localized adjacent to terminal bronchi, and diminished by prior LPS exposure.DiscussionThese results suggest a pivotal role for pulmonary macrophage ontogeny in modulating allergic airway responses. Moreover, our findings highlight the implications of prior environmental exposures in shaping future immune responses and influencing the development of allergies. By elucidating the mechanisms underlying these phenomena, this study provides valuable insights into potential therapeutic targets for allergic airway diseases and avenues for further research into immune modulation and allergic disease prevention.
وصف الملف: electronic resource
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2دورية أكاديمية
المؤلفون: Clair E. Weidgang, Ronan Russell, Purushothama R. Tata, Susanne J. Kühl, Anett Illing, Martin Müller, Qiong Lin, Cornelia Brunner, Tobias M. Boeckers, Kerstin Bauer, Apriliana E.R. Kartikasari, Yanchun Guo, Melanie Radenz, Christof Bernemann, Matthias Weiß, Thomas Seufferlein, Martin Zenke, Michelina Iacovino, Michael Kyba, Hans R. Schöler, Michael Kühl, Stefan Liebau, Alexander Kleger
المصدر: Stem Cell Reports, Vol 2, Iss 5, p 747 (2014)
مصطلحات موضوعية: Medicine (General), R5-920, Biology (General), QH301-705.5
وصف الملف: electronic resource
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المؤلفون: Robert M. Tighe, Anastasiya Birukova, Yuryi Malakhau, Yoshihiko Kobayashi, Aaron T. Vose, Vidya Chandramohan, Jaime M. Cyphert-Daly, R. Ian Cumming, Helene Fradin Kirshner, Purushothama R. Tata, Jennifer L. Ingram, Michael D. Gunn, Loretta G. Que, Yen-Rei A. Yu
المصدر: bioRxiv
مصطلحات موضوعية: Article
الوصف: The ontogenetic composition of tissue-resident macrophages following injury, environmental exposure, or experimental depletion can be altered upon re-establishment of homeostasis. However, the impact of altered resident macrophage ontogenetic milieu on subsequent immune responses is poorly understood. Hence, we assessed the effect of macrophage ontogeny alteration following return to homeostasis on subsequent allergic airway responses to house dust mites (HDM). Using lineage tracing, we confirmed alveolar and interstitial macrophage ontogeny and their replacement by bone marrow-derived macrophages following LPS exposure. This alteration in macrophage ontogenetic milieu reduced allergic airway responses to HDM challenge. In addition, we defined a distinct population of resident-derived interstitial macrophages expressing allergic airway disease genes, located adjacent to terminal bronchi, and reduced by prior LPS exposure. These findings support that the ontogenetic milieu of pulmonary macrophages is a central factor in allergic airway responses and has implications for how prior environmental exposures impact subsequent immune responses and the development of allergy.
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::51e02b009ea1f09885fdcfe301f16c3f
https://europepmc.org/articles/PMC9949163/ -
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المؤلفون: Ansley S. Conchola, Tristan Frum, Zhiwei Xiao, Peggy P. Hsu, Renee F.C. Hein, Alyssa Miller, Yu-Hwai Tsai, Angeline Wu, Kamika Kaur, Emily M. Holloway, Abhinav Anand, Preetish K. L. Murthy, Ian Glass, Purushothama R. Tata, Jason R. Spence
الوصف: Recent advances using single cell genomic approaches have identified new epithelial cell types and uncovered cellular heterogeneity in the murine and human lung (1). Here, using scRNA-seq and microscopy we identify and describe a secretory-like cell that is enriched in the small airways of the developing human lung and identified by the unique co-expression of SCGB3A2/SFTPB/CFTR. To place these cells in the hierarchy of airway development, we apply a single cell barcode-based lineage tracing method track the fate of SCGB3A2/SFTPB/CFTR cells during airway organoid differentiation in vitro (2). Lineage tracing revealed that these cells have distinct developmental potential from basal cells, giving rise predominantly to pulmonary neuroendocrine cells (PNECs) and a subset of multiciliated cells distinguished by high C6 and low MUC16 expression. We conclude that SCGB3A2/SFTPB/CFTR cells act as a progenitor cell contributing to the cellular diversity and heterogeneity in the developing human airway.SIGNIFICANCE STATEMENTThe current study identifies a novel secretory cell type that is present predominantly in the small airway of the developing human lung. These secretory cells are defined by co-expression of SCGB3A2/SFTPB/CFTR, and functional studies show that this cell gives rise to pulmonary neuroendocrine cells and a sub-population of multiciliated cells, thereby leading to cellular heterogeneity.
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_________::2e037f265c82ad85dc07220adcff7cf3
https://doi.org/10.1101/2022.06.13.495813 -
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المؤلفون: Léa El Haddad, Elias Lai, Preetish Murthy, Purushothama R. Tata, Mai K. ElMallah
المصدر: The FASEB Journal. 36
مصطلحات موضوعية: Genetics, Molecular Biology, Biochemistry, Biotechnology
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