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1دورية أكاديمية
المؤلفون: Aoming Cheng, Qiaoshi Xu, Bo Li, Lirui Zhang, Hao Wang, Chang Liu, Zhengxue Han, Zhien Feng
المصدر: Cell Death and Disease, Vol 15, Iss 5, Pp 1-14 (2024)
الوصف: Abstract The tumor margin as the invasive front has been proven to be closely related to the progression and metastasis of oral squamous cell carcinoma (OSCC). However, how tumor cells in the marginal region obtain the extra energy needed for tumor progression is still unknown. Here, we used spatial metabolomics and the spatial transcriptome to identify enhanced energy metabolism in the tumor margin of OSCC and identified that the downregulation of Ras-related glycolysis inhibitor and calcium channel regulator (RRAD) in tumor cells mediated this process. The absence of RRAD enhanced the ingestion of glucose and malignant behaviors of tumor cells both in vivo and in vitro. Mechanically, the downregulation of RRAD promoted the internal flow of Ca2+ and elevated its concentration in the nucleus, which resulted in the activation of the CAMKIV-CREB1 axis to induce the transcription of the glucose transporter GLUT3. GLUT inhibitor-1, as an inhibitor of GLUT3, could suppress this vigorous energy metabolism and malignant behaviors caused by the downregulation of RRAD. Taken together, our study revealed that enhanced energy metabolism in the tumor margin mediated by RRAD promotes the progression of OSCC and proved that GLUT3 is a potential target for future treatment of OSCC.
وصف الملف: electronic resource
Relation: https://doaj.org/toc/2041-4889
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2دورية أكاديمية
المؤلفون: Qiaoshi Xu, Aoming Cheng, Bo Li, Xutengyue Tian, Zhengxue Han, Zhien Feng
المصدر: Journal of Extracellular Vesicles, Vol 13, Iss 4, Pp n/a-n/a (2024)
مصطلحات موضوعية: ATP‐citrate lyase, C‐X‐C motif chemokine receptor 1, head and neck squamous cell carcinoma, heterogeneity, small extracellular vesicles, Cytology, QH573-671
الوصف: Abstract The heterogeneity of tumour cells enables cancers to dynamically adapt to microenvironmental stresses during progression. However, the mechanism underlying the transformation and intercellular communication between heterogeneous tumour cells has remained elusive. Here, we report a “contagion model” that mediates intercellular transformation between heterogeneous tumour cells which facilitates tumour progression. Initially identifying heterogeneous expression of CXCR1, a receptor for interleukin‐8, in head and neck squamous cell carcinoma (HNSCC) tumour cells, we found that CXCR1High tumour cells had higher abilities for migration and invasion. Following interleukin‐8‐mediated activation, CXCR1High cells transformed CXCR1Low cells into CXCR1High cells through the secretion of small extracellular vesicles (sEVs), which increased the proportion of CXCR1High cells and facilitated tumour progression. Mechanistically, we demonstrate that sEVs derived from interleukin‐8‐activated CXCR1High cells contain high levels of ATP citrate lyase (ACLY), which acetylates NF‐κB p65 and facilitates its nuclear translocation to transcribe CXCR1 in CXCR1Low cells. That process could be inhibited by Bempedoic acid, an FDA‐approved ACLY‐targeted drug. Taken together, our study reveals an sEV‐mediated transformation of CXCR1Low to CXCR1High cells that promotes HNSCC progression. This provides a new paradigm to explain the dynamic changes of heterogeneous tumour cells, and identifies Bempedoic acid as a potential drug for HNSCC treatment.
وصف الملف: electronic resource
Relation: https://doaj.org/toc/2001-3078
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3دورية أكاديمية
المؤلفون: Lirui Zhang, Qiaoshi Xu, Huan Liu, Bo Li, Hao Wang, Chang Liu, Jinzhong Li, Bin Yang, Lizheng Qin, Zhengxue Han, Zhien Feng
المصدر: Frontiers in Oncology, Vol 12 (2022)
مصطلحات موضوعية: salvage surgery, massive soft tissue flap, recurrent/metastatic head and neck cancer, quality of life, overall survival (OS), Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282
الوصف: ObjectivesThe prognosis, choice of reconstruction and the quality of life (QOL) after salvage surgery (SS) for extensively locoregional recurrent/metastatic head and neck cancer (R/M HNC) is an important issue, but there are few reports at present.Materials and methodsWe analyzed extensively locoregional R/M HNC patients from March 1, 2015, to December 31, 2021 who underwent SS with latissimus dorsi or pectoralis major musculocutaneous flaps. QOL were accessed using QLQ-H&N35 and UW-QOL questionnaire. Wilcoxon signed-rank test was used to compare difference between pre- and post-QOL and Kaplan-Meier curves were used in estimate overall survival (OS) and disease-free survival (DFS). The literature review summarized recent 10 years clinical trials of nonoperative treatment in R/M head and neck cancer.Results1362 patients were identified and 25 patients were analyzed after screened. Median age at surgery was 59 years (range 43-77), 15/25(60%) were male and 22/25(88%) chose latissimus dorsi flap. Better mean pain score after applying massive soft tissue flaps revealed relief of severe pain(p
وصف الملف: electronic resource
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4دورية أكاديمية
المؤلفون: Hanyue Chang, Qiaoshi Xu, Jiayi Li, Mingyu Li, Zhiyuan Zhang, Hailong Ma, Xi Yang
المصدر: Cell Death and Disease, Vol 12, Iss 8, Pp 1-13 (2021)
الوصف: Abstract Pyruvate kinase M2 as a key rate-limiting enzyme in glycolysis, it plays a critical role in metabolic reprogramming and carcinogenesis. However, whether PKM2 can promote immunosuppressive microenvironment formation remains unknown in head and neck squamous cell carcinoma (HNSCC). PKM2 expression was detected using immunohistochemical staining. The biological functions of PKM2 were investigated in vitro and in vivo. Lactate production and the expression of Galectin-9, a critical immunosuppression molecule, were detected after PKM2 knockdown and overexpression in HNSCC cells. The mechanism of lactate regulating Galectin-9 expression through NF-κB signaling was explored in vitro. Overexpression of PKM2 correlates with poor prognosis in HNSCC patients. Silencing PKM2 markedly inhibits proliferation and metastasis capacity in vivo and in vitro, and vice versa. The glycolysis and glycolytic capacity are significantly decreased after PKM2 silencing. Lactate secretion induced by PKM2 significantly promotes migration and invasion capacity. Furthermore, a positive correlation between PKM2 and Galectin-9 expression is observed in HNSCC tissues. The induction of Galectin-9 expression by PKM2 can be affected by a lactate transporter inhibitor. Mechanically, lactate impeded the suppressive transcriptional complex formation of NF-κB and histone deacetylase 3 (HDAC3), which released the transcription of Galectin-9 mediated by NF-κB signaling. Our findings demonstrate that lactate produced by PKM2 upregulation promotes tumor progression and Galectin-9-mediated immunosuppression via NF-κB signaling inhibition in HNSCC, which bridges metabolism and immunosuppression. The novel PKM2-lactate-Galectin-9 axis might be a potential therapeutic target in HNSCC.
وصف الملف: electronic resource
Relation: https://doaj.org/toc/2041-4889
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5دورية أكاديمية
المؤلفون: Qiaoshi Xu, Chong Wang, Xiaohong Yuan, Zhien Feng, Zhengxue Han
المصدر: Translational Oncology, Vol 10, Iss 1, Pp 10-16 (2017)
مصطلحات موضوعية: Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282
الوصف: BACKGROUND: The prognostic value of tumor-infiltrating lymphocytes (TILs) in head and neck squamous cell carcinoma (HNSCC) remains controversial. Additionally, there is no standardized approach or cutoff value for evaluating TIL levels. The aim of this study was to establish a feasible method and criterion to assess TIL levels for future clinical practice and research use and to explore the relationship between TIL levels and prognosis. PATIENTS AND METHODS: This retrospective cohort study reviewed the records and pathological sections of 202 patients with HNSCC who were surgically treated at Beijing Stomatological Hospital, Capital Medical University, from January 1998 to January 2011. The predictor variable was the TIL level. The main outcome assessment parameters were disease-free survival (DFS) and disease-specific survival (DSS). RESULT: The T stage (P = .008), smoking history (P = .042), alcohol history (P = .048), need for radiotherapy (P = .012) and microscopic extracapsular spread (ECS) (P = .012) were associated with the TIL level. A cutoff value equal to 70% could be taken as a threshold for TIL assessment, with a TIL level higher than 70% associated with a better prognosis (DFS rate: 51.9%, P = .018; DSS rate: 59.3%, P = .049). The Cox regression model showed that the TIL level was an independent prognostic factor for DFS (hazard ratio (HR): 0.786, 95% CI: 0.618-0.999, P = .049). CONCLUSION: The TIL level is closely related to the prognosis of patients with HNSCC. A threshold value of 70% is appropriate for TIL assessment, as patients with a TIL level higher than 70% show a better prognosis. Thus, the TIL level might serve as an independent predictor for HNSCC recurrence.
وصف الملف: electronic resource
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المؤلفون: Delong Li, Qifang Niu, Chong Wang, Wei Wei, Bo Li, Huan Liu, Ranran Xiao, Hao Wang, Qiaoshi Xu, Zhengxue Han, Zhien Feng
المصدر: Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology. 135:33-41
مصطلحات موضوعية: Postoperative Complications, Squamous Cell Carcinoma of Head and Neck, Head and Neck Neoplasms, Humans, Radiology, Nuclear Medicine and imaging, Dentistry (miscellaneous), Surgery, Prospective Studies, Length of Stay, Oral Surgery, Retrospective Studies, Pathology and Forensic Medicine
الوصف: We aimed to validate and evaluate the comprehensive complication index (CCI) as a measure of postoperative complications (POCs) in the patients with head and neck squamous cell carcinoma (HNSCC) who had undergone free-flap reconstruction.A single-center, prospective cohort study was conducted on the patients with primary HNSCC treated between 2015 and 2020.Of 443 patients, 177 experienced POCs (40.0%). According to the new CCI, 93 patients had severe POCs (score ≥26.2; 21.0%), and only 76 patients were classified as having severe POCs (grade ≥III; 17.2%) according to the traditional Clavien-Dindo classification (CDC). The patients with CCI scores ≥26.2 had a higher rate of major adverse short-term outcomes, such as prolonged length of the hospital stay after surgery, excessive medical costs, and the need for transfusion. Importantly, the Kaplan-Meier analysis revealed a lower overall survival rate for patients with severe complications (CCI score ≥26.2, P = .038). However, no statistically significant differences were observed between CDC grades III or above and overall survival (P = .100).The CCI can be used to evaluate POCs of free-flap reconstruction in patients with HNSCC. It performed more accurately than the CDC in short- and long-term outcome prediction and risk factor identification.
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7
المؤلفون: Bo Li, Aoming Cheng, Huan Liu, Hao Wang, Chong Wang, Qiaoshi Xu, Zhengxue Han, Zhien Feng
المصدر: Oral Diseases.
مصطلحات موضوعية: Otorhinolaryngology, General Dentistry
الوصف: The purpose of this study is to explore the effects of modified vermilion border-marionette line (MVBML) approach on postoperative facial scar, nerves injury, and prognosis of patients with buccal squamous cell carcinoma (BSCC).This is a single-center, prospective cohort study that enrolled 80 patients with BSCC from June 2015 to December 2020. According to the different surgical approaches, the patients were divided into two groups: the lower lip median (LLM) approach group and the MVBML approach group.The results showed that the appearance (p = 0.003), scar consciousness (p 0.001) and satisfaction with appearance (p = 0.001) of patients in the MVBML group were significantly better than those in the LLM group, and the difference was more obvious in elderly group. Statistical analysis of postoperative nerves injury showed that the MVBML group had a lower risk of facial and mental nerves injury than the LLM group, and there was a significant statistical difference in mental nerve injury between the two groups (p 0.001). Through Kaplan-Meier survival analysis, we found no significant difference in disease-specific survival (p = 0.47) or disease-free survival (p = 0.70) between the LLM approach group and the MVBML approach group.The MVBML surgical approach is worthy of advancement for the surgical treatment of BSCC.
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8
المؤلفون: null Bo Li, null Aoming Cheng, null Huan Liu, null Hao Wang, null Chong Wang, null Qiaoshi Xu, null Zhengxue Han, null Zhien Feng
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_________::83e368fa2f66aa91f6b309efc0c2da05
https://doi.org/10.1111/odi.14340/v2/response1 -
9
المؤلفون: Ranran Xiao, Jinghan Wu, Brent B. Ward, Huan Liu, Bo Li, Chong Wang, Qiaoshi Xu, Zhengxue Han, Zhien Feng
المصدر: Oral Diseases.
مصطلحات موضوعية: Otorhinolaryngology, General Dentistry
الوصف: The purpose of this study was to investigate the prognostic value of the family history of cancer (FHC) in predicting survival and clinicopathological features in oral squamous cell carcinoma (OSCC) patients.This single-institution study utilized data from 610 patients undergoing surgery from 2014 to 2020 that was prospectively collected and cataloged for research purposes. All patients underwent standard surgery with/without radiotherapy or chemoradiotherapy. We statistically evaluated whether FHC was associated with changes in disease-free survival (DFS) and disease-specific survival (DSS).Among 610 patients, 141 (23.1%) reported a family history of cancer. The distribution of clinicopathological characteristics was balanced between FHC-positive and FHC-negative OSCC patients. FHC-positive patients had decreased DFS (p = 0.005) and DSS (p = 0.018) compared to FHC-negative patients.FHC-positive OSCC patients have a poorer prognosis. FHC positivity is an independent predictor of negative outcomes based on DFS and DSS. FHC should be a consideration in screening, evaluating, counseling, and treating OSCC patients.
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10
المؤلفون: Jia Kang, Qiaoshi Xu, Xiaohong Yuan, Zhengxue Han, Ming Su, Chong Wang
مصطلحات موضوعية: Prognostic factor, business.industry, Tongue squamous cell carcinoma, Tumour stroma, Cancer research, Medicine, business
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_________::d2c52770a5822ab7fe885ea8e3a2df8f
https://doi.org/10.1111/odi.14013/v2/response1
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