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1دورية أكاديمية
المؤلفون: Juliana Campo Garcia, Roemel Jeusep Bueno, Maren Salla, Ivette Martorell-Serra, Bibiane Seeger, Nilufar Akbari, Pia Sperber, Harald Stachelscheid, Carmen Infante-Duarte, Friedemann Paul, Sarah C. Starossom
المصدر: Scientific Reports, Vol 14, Iss 1, Pp 1-15 (2024)
مصطلحات موضوعية: Human neural stem cells, Monocytes, Macrophages, Neuroinflammation, High-content, Medicine, Science
الوصف: Abstract During neuroinflammation, monocytes that infiltrate the central nervous system (CNS) may contribute to regenerative processes depending on their activation status. However, the extent and mechanisms of monocyte-induced CNS repair in patients with neuroinflammatory diseases remain largely unknown, partly due to the lack of a fully human assay platform that can recapitulate monocyte-neural stem cell interactions within the CNS microenvironment. We therefore developed a human model system to assess the impact of monocytic factors on neural stem cells, establishing a high-content compatible assay for screening monocyte-induced neural stem cell proliferation and differentiation. The model combined monocytes isolated from healthy donors and human embryonic stem cell derived neural stem cells and integrated both cell-intrinsic and -extrinsic properties. We identified CNS-mimicking culture media options that induced a monocytic phenotype resembling CNS infiltrating monocytes, while allowing adequate monocyte survival. Monocyte-induced proliferation, gliogenic fate and neurogenic fate of neural stem cells were affected by the conditions of monocytic priming and basal neural stem cell culture as extrinsic factors as well as the neural stem cell passage number as an intrinsic neural stem cell property. We developed a high-content compatible human in vitro assay for the integrated analysis of monocyte-derived factors on CNS repair.
وصف الملف: electronic resource
Relation: https://doaj.org/toc/2045-2322
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2دورية أكاديمية
المؤلفون: Silvina Romero-Suárez, Alba Del Rio Serrato, Roemel Jeusep Bueno, Daniel Brunotte-Strecker, Christina Stehle, Caio Andreeta Figueiredo, Laura Hertwig, Ildiko R. Dunay, Chiara Romagnani, Carmen Infante-Duarte
المصدر: Frontiers in Immunology, Vol 10 (2019)
مصطلحات موضوعية: innate lymphoid cells (ILCs), ILC1s, natural killer (NK) cells, central nervous system (CNS), experimental autoimmune encephalomyelitis (EAE), mouse, Immunologic diseases. Allergy, RC581-607
الوصف: Innate lymphoid cells (ILCs) are tissue resident cells with organ-specific properties. Here, we show that the central nervous system (CNS) encompasses ILCs. In particular, CD3−NK1.1+ cells present in the murine CNS comprise natural killer (NK) cells, ILC1s, intermediate ILC1s (intILC1s) and ex-ILC3s. We investigated the properties of CNS-ILC1s in comparison with CNS-NK cells during steady state and experimental autoimmune encephalomyelitis (EAE). ILC1s characteristically express CXCR3, CXCR6, DNAM-1, TRAIL, and CD200R and display heightened TNF-α production upon stimulation. In addition, ILC1s express perforin and are able to degranulate, although in a lesser extent than NK cells. Within the CNS compartments, ILC1s are enriched in the choroid plexus where very few NK cells are present, and also reside in the brain parenchyma and meninges. During EAE, ILC1s maintain stable IFN-γ and TNF-α levels while in NK cells the production of these cytokines increases as EAE progresses. Moreover, the amount of ILC1s and intILC1s increase in the parenchyma during EAE, but in contrast to NK cells, they show no signs of local proliferation. The upregulation in the inflamed brain of chemokines involved in ILC1 migration, such as CXCL9, CXCL10, and CXCL16 may lead to a recruitment of ILC1s from meninges or choroid plexus into the brain parenchyma. In sum, CNS-ILC1 phenotype, distribution and moderate inflammatory response during EAE suggest that they may act as gatekeepers involved in the control of neuroinflammation.
وصف الملف: electronic resource
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المؤلفون: John Noel Viaña, Mario Carlo Severo, Miguel Barretto-Garcia, Paul James Magtaan, Jason Tan Liwag, Roemel Jeusep Bueno, Christer de Silva, Ruby Shaira Panela
المصدر: Queering Science Communication ISBN: 9781529224436
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8db2baf4f6726d46610acd37f32c5d86
https://doi.org/10.56687/9781529224436-021 -
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المؤلفون: Frederic Gilbert, Roemel Jeusep Bueno, John Noel M. Viaña
المصدر: The American Journal of Bioethics. 17:24-26
مصطلحات موضوعية: Treatment response, medicine.medical_specialty, Health Policy, Genetic counseling, Public health, education, Genomics, 06 humanities and the arts, 0603 philosophy, ethics and religion, Mental health, 03 medical and health sciences, Issues, ethics and legal aspects, 0302 clinical medicine, Genotype, medicine, 060301 applied ethics, Psychology, Association (psychology), Neuroethics, 030217 neurology & neurosurgery, Clinical psychology
الوصف: Kong, Dunn, and Parker (2017) highlight several ethical issues associated with the translation of genomic research findings into public health and clinical contexts. Among the ethical concerns they...
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المؤلفون: Eva Maria Cutiongco-de la Paz, Orlino C. Bisquera, Pedrito Y. Tagayuna, Eleanor A. Dominguez, Arnold Joseph M. Fernandez, Jose B. Nevado, Carmencita D. Padilla, Frances Maureen C. Rocamora, Rosalyn Hernandez-Sebastian, Maria Noemi G. Pato, Roemel Jeusep Bueno, Eriberto R. Layda, Catherine Lynn T. Silao, Sullian Sy-Naval, Nelia Tan-Liu, Richmond B. Ceniza, Elizabeth A. Nuqui, Richard C. Tia, Reynaldo O. Joson, Oliver G. Florendo, Rey A. Desales, Conrado C. Cajucom, Higinio T. Mappala, Alberto B. Roxas, Aileen David-Wang, Luminardo M. Ramos, Florentino C. Doble, Roberto M. Montevirgen, Sergio P. Paguio, Jonathan M. Asprer, Ma. Cecilia M. Sison, Tristan Chipongian, Francisco T. Roxas, Virgilio P. Banez, Alex C. Tapia, Hans Francis D. Ferraris, Adonis A. Guancia, Albert B. Albay, Andrew D. Dimacali, Emmanuel F. Montana, Benito B. Bionat, Maria Constancia Obrerro-Carrillo, Catalina de Siena Gonda-Dimayacyac, Lakan U. Beratio, Joselito F. David, John A. Coloma, Leander Linus Philip P. Simpao, Corazon A. Ngelangel
المصدر: Acta Medica Philippina. 51
مصطلحات موضوعية: Oncology, medicine.medical_specialty, CYP2D6, business.industry, Cancer, Single-nucleotide polymorphism, General Medicine, medicine.disease, Internal medicine, Genotype, medicine, SNP, Biomarker (medicine), business, Lung cancer, Pharmacogenetics
الوصف: Objectives. The highly polymorphic nature of the CYP2D6 gene and its central role in the metabolism of commonly used drugs make it an ideal candidate for pharmacogenetic screening. This study aims to determine the prevalence of CYP2D6 polymorphisms among Filipinos and their association to lung cancer. Method. Forty seven single nucleotide polymorphisms (SNPs) of the CYP2D6 gene were genotyped from DNA samples of 115 cases with lung cancer and age- and sex-matched 115 controls. Results. Results show that 18 out of 47 polymorphisms have significant genotypic variability (>1% for at least 2 genotypes). No variant is associated with lung cancer. However, rs1135840, rs16947 and rs28360521, were found to be highly variable among Filipinos. Conclusion. This study demonstrated that CYP2D6 polymorphisms are present among Filipinos, which, although not found to be associated with lung cancer, can be useful biomarkers for future pharmacogenetic studies. The SNP rs16947 is found to be associated with cancer and timolol-induced bradycardia; the SNP rs1135840, on the other hand, is only shown to be linked with cancer. The genetic variant rs28360521 is known to be associated with low-dose aspirin-induced lower gastrointestinal bleeding.
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_________::0937e5a893a39744e12e805d3d8601ec
https://doi.org/10.47895/amp.v51i3.550
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