المؤلفون: Marcelo V. S. Alves, Lanaia I. L. Maciel, Ruver R. F. Ramalho, Leomir A. S. Lima, Boniek G. Vaz, Camilo L. M. Morais, João O. S. Passos, Rodrigo Pegado, Kássio M. G. Lima

المصدر: Scientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)

مصطلحات موضوعية: Medicine, Science

الوصف: Abstract Fibromyalgia is a rheumatological disorder that causes chronic pain and other symptomatic conditions such as depression and anxiety. Despite its relevance, the disease still presents a complex diagnosis where the doctor needs to have a correct clinical interpretation of the symptoms. In this context, it is valid to study tools that assist in the screening of this disease, using chemical work techniques such as mass spectroscopy. In this study, an analytical method is proposed to detect individuals with fibromyalgia (n = 20, 10 control samples and 10 samples with fibromyalgia) from blood plasma samples analyzed by mass spectrometry with paper spray ionization and subsequent multivariate classification of the spectral data (unsupervised and supervised), in addition to the treatment of selected variables with possible associations with metabolomics. Exploratory analysis with principal component analysis (PCA) and supervised analysis with successive projections algorithm with linear discriminant analysis (SPA-LDA) showed satisfactory results with 100% accuracy for sample prediction in both groups. This demonstrates that this combination of techniques can be used as a simple, reliable and fast tool in the development of clinical diagnosis of Fibromyalgia.

وصف الملف: electronic resource

Relation: https://doaj.org/toc/2045-2322

URL الوصول: https://doaj.org/article/e2bce6dbd4b643dba89e88019d0668ef

المؤلفون: Igor Pereira, Ruver R. F. Ramalho, Lanaia I. L. Maciel, Deborah V. A. de Aguiar, Yan Trindade, Georgiana F. da Cruz, Angelo Marinho Vianna, Iris M. Júnior, Gesiane da S. Lima, Boniek G. Vaz

المصدر: Analytical Chemistry. 94:13691-13699

مصطلحات موضوعية: Minerals, Spectrometry, Mass, Electrospray Ionization, Fatty Acids, Laser Therapy, Disaccharides, Hydrocarbons, Analytical Chemistry

الوصف: Here, we present a new application of desorption electrospray ionization (DESI) and laser ablation electrospray ionization (LAESI) mass spectrometry imaging to assess the spatial location of organic compounds, both polar and nonpolar, directly from rock surfaces. Three carbonaceous rocks collected from an aquatic environment and a berea sandstone subjected to a small-scale oil recovery experiment were analyzed by DESI and LAESI. No rock pretreatment was required before DESI and LAESI analyses. DESI detected and spatially mapped several fatty acids and a disaccharide on the surfaces of carbonaceous rocks, and various nitrogenated and oxygenated compounds on the surfaces of berea sandstone. In contrast, LAESI using a 3.4 μm infrared laser beam was able to detect and map hydrocarbons on the surfaces of all rock samples. Both techniques can be combined to analyze polar and nonpolar compounds. DESI can be used first to detect polar compounds, as it does not destroy the rock surface, and LAESI can then be used to analyze nonpolar analytes, as it destroys a layer of the sample surface. Both techniques have the potential to be used in several scientific areas involving rocks and minerals, such as in the analysis of industry-derived contaminants in aquatic sediments or in small-scale rock-fluid interaction experiments.

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba9b4bf66435769ffa188667964f19b1
https://doi.org/10.1021/acs.analchem.2c01154