المؤلفون: Chinenov DV; Institute for Urology and Reproductive Health, Sechenov University, Moscow, Russia., Shpot EV; Institute for Urology and Reproductive Health, Sechenov University, Moscow, Russia., Chernov YN; Institute for Urology and Reproductive Health, Sechenov University, Moscow, Russia., Gerasimov AN; Institute for Urology and Reproductive Health, Sechenov University, Moscow, Russia., Kazachevskaya LY; The Pathology Residency Program at Louisiana State University Health Sciences Center in Shreveport, LA, USA., Lyapichev KA; Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA., Ismailov HM; Institute for Urology and Reproductive Health, Sechenov University, Moscow, Russia., Tsukkiev ZK; Institute for Urology and Reproductive Health, Sechenov University, Moscow, Russia., Korolev DO; Institute for Urology and Reproductive Health, Sechenov University, Moscow, Russia., Rapoport LM; Institute for Urology and Reproductive Health, Sechenov University, Moscow, Russia.

المصدر: Urologia [Urologia] 2024 May; Vol. 91 (2), pp. 311-319. Date of Electronic Publication: 2024 Jan 21.

نوع المنشور: Journal Article; Comparative Study

بيانات الدورية: Publisher: Sage Publications Country of Publication: United States NLM ID: 0417372 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1724-6075 (Electronic) Linking ISSN: 03915603 NLM ISO Abbreviation: Urologia Subsets: MEDLINE

مواضيع طبية MeSH: Prostatic Neoplasms*/radiotherapy , Prostatic Neoplasms*/pathology , Prostatic Neoplasms*/surgery , Prostatic Neoplasms*/therapy , Cryosurgery*/methods , Brachytherapy*/adverse effects, Humans ; Male ; Aged ; Treatment Outcome ; Middle Aged ; Minimally Invasive Surgical Procedures ; Quality of Life ; Retrospective Studies

مستخلص: Objectives: To study the functional and oncological results of minimally invasive treatment methods: cryoablation, brachytherapy, and high-intensity focused ultrasound (HIFU) therapy of localized prostate cancer in a single hospital.
Methods: One hundred sixty patients with localized prostate cancer were treated with minimally invasive methods (53, 52, 55 patients with cryoablation, brachytherapy and HIFU therapy, respectively). Prostate-specific antigen and evaluation of post-procedure biopsies were used as an assessment. The review of functional indicators and quality of life was made with International Index of Erectile Function (IIEF-5), International Prostate Symptom Score (IPSS), maximum urinary flow rate (Qmax), quality of life (QoL) questionnaires.
Results: Patients after cryoablation showed worse results of oncological control according to positive repeat biopsies, best indicators were observed after brachytherapy. When considering the IPSS results, there were statistically significantly worse scores in the brachytherapy group in the early postoperative period, these differences do not reach statistical significance in the late period in the brachytherapy and cryoablation groups. Patients in the cryoablation group showed worse indicators of IIEF-5; in the early postoperative period; in the late follow-up period, the indicators of erectile function in patients in the cryoablation group did not statistically significantly differ from those in patients after brachytherapy. Patients after HIFU therapy showed fewer cases of de novo erectile dysfunction during the follow-up period of 3 years, higher average IIEF-5 scores, lower IPSS scores and better QoL results.
Conclusion: The recurrence of prostate cancer was statistically significantly higher in the International Society of Urological Pathology (ISUP) 3 grade group. HIFU therapy had better urination indicators compared to other groups, that can be associated with the laser enucleation of prostatic hyperplasia. The advantage was noted in patients after HIFU therapy when comparing the parameters to the IIEF-5 thus, HIFU treatment had a better impact on patients' QoL with localized prostate cancer.
Competing Interests: Declaration of conflicting interestsThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.

المؤلفون: Damiev AD; FGAOU I.M. Sechenov First Moscow State Medical University, Moscow, Russia., Shpot EV; FGAOU I.M. Sechenov First Moscow State Medical University, Moscow, Russia., Akopyan GN; FGAOU I.M. Sechenov First Moscow State Medical University, Moscow, Russia., Dymov AM; FGAOU I.M. Sechenov First Moscow State Medical University, Moscow, Russia., Kharchilava RR; FGAOU I.M. Sechenov First Moscow State Medical University, Moscow, Russia., Yandiev SA; FGAOU I.M. Sechenov First Moscow State Medical University, Moscow, Russia., Ismailov KM; FGAOU I.M. Sechenov First Moscow State Medical University, Moscow, Russia., Lerner YV; FGAOU I.M. Sechenov First Moscow State Medical University, Moscow, Russia., Kammaev KA; FGAOU I.M. Sechenov First Moscow State Medical University, Moscow, Russia., Gazimiev MA; FGAOU I.M. Sechenov First Moscow State Medical University, Moscow, Russia.

المصدر: Urologiia (Moscow, Russia : 1999) [Urologiia] 2023 Sep (4), pp. 24-29.

نوع المنشور: English Abstract; Journal Article

بيانات الدورية: Publisher: Medit︠s︡ina Country of Publication: Russia (Federation) NLM ID: 100900900 Publication Model: Print Cited Medium: Print ISSN: 1728-2985 (Print) Linking ISSN: 17282985 NLM ISO Abbreviation: Urologiia Subsets: MEDLINE

مواضيع طبية MeSH: Carbon Dioxide* , Urinary Bladder Neoplasms*/diagnosis, Humans ; Pilot Projects ; Urologic Surgical Procedures ; Cystoscopy

مستخلص: Aim: To evaluate the possibility of performing transurethral carboxycryobiopsy (CCB) and carboxycryoextraction (CCE) of a bladder tumor for pathomorphological examination, as well as to perform a comparative analysis of the safety (quality) of biopsy material (tumor tissue) during standard transurethral biopsy and carboxycryobiopsy.
Materials and Methods: In the first experiment in vitro, CCE of bladder tumor fragments obtained after transurethral resection was performed. In the second pilot study, cystoscopy followed by CCB and CCE in a patient with multiple bladder tumors was done. The procedure was performed by transurethral access. During cryopreservation of the bladder tumor, a biopsy was performed. After freezing, the tumor was removed from the bladder and sent for histological examination.
Results: The first experiment showed that cryoextraction of the fragments of a bladder tumor using carbon dioxide (CCE) in vitro is a feasible procedure and allows the evacuation of tumor tissues of various sizes. According to the second experiment, CCB and CCE of the bladder tumor using carbon dioxide allows to obtain a biopsy of a bladder tumor of sufficient size without compression or coagulation artifacts, which contributes to a more accurate histological evaluation.
Conclusion: Our experiments showed that CCB and CCE of a bladder tumor using carbon dioxide are feasible procedures that contribute to obtaining better biopsy material for pathomorphological examination, and also allows to evaluate the effect of low temperatures of carbon dioxide on the biopsy material (tumor tissue).

المؤلفون: Kurbanov AA; Institute for Urology and Human Reproductive Health of FGAOU I.M. Sechenov First Moscow State Medical University, Moscow, Russia., Kryukov SR; Institute for Urology and Human Reproductive Health of FGAOU I.M. Sechenov First Moscow State Medical University, Moscow, Russia., Chernov DV; Institute for Urology and Human Reproductive Health of FGAOU I.M. Sechenov First Moscow State Medical University, Moscow, Russia., Votyakov AY; Institute for Urology and Human Reproductive Health of FGAOU I.M. Sechenov First Moscow State Medical University, Moscow, Russia., Shpot EV; Institute for Urology and Human Reproductive Health of FGAOU I.M. Sechenov First Moscow State Medical University, Moscow, Russia.

المصدر: Urologiia (Moscow, Russia : 1999) [Urologiia] 2023 Sep (4), pp. 125-128.

نوع المنشور: Case Reports; English Abstract; Journal Article

بيانات الدورية: Publisher: Medit︠s︡ina Country of Publication: Russia (Federation) NLM ID: 100900900 Publication Model: Print Cited Medium: Print ISSN: 1728-2985 (Print) Linking ISSN: 17282985 NLM ISO Abbreviation: Urologiia Subsets: MEDLINE

مواضيع طبية MeSH: Carcinoma, Renal Cell*/surgery , Carcinoma, Renal Cell*/pathology , Ureter*/surgery , Ureter*/pathology , Robotic Surgical Procedures* , Carcinoma, Transitional Cell*/surgery , Robotics* , Urinary Bladder Neoplasms*/surgery , Kidney Neoplasms*/pathology, Humans ; Nephrectomy

مستخلص: Renal cell carcinoma (RCC) accounts for more than 90% of cases of malignant kidney tumors and represents 2-3% of all malignancies worldwide. Clear cell renal cell carcinoma (ccRCC), the most common type of RCC, comprising 70-80% of cases. RCC most commonly metastasizes to the lungs, bones, lymph nodes, liver, adrenal glands, and brain. Synchronous metastasis of RCC to the ipsilateral ureter represents an extremely rare event. Ureteral metastasis is a significant diagnostic challenge, since it is quite difficult to determine whether it has metastatic origin (RCC) or it is a primary urothelial tumor. Moreover, due to the rarity of disease, treatment strategy is not well established. We present a rare case of patient with the RCC of a single left kidney and metachronous metastasis to the ipsilateral ureter that was initially assumed to be primary urothelial carcinoma. The robotic-assisted left-side partial nephrectomy with a segmental resection of left lower ureter and Boari reconstruction was performed. This case of successful treatment with robotic-assisted approach shows a great organ-sparing potential of robotic surgery in the treatment of complex oncological patients for whom it is extremely important to preserve the maximum volume of functioning renal tissue, particularly in those with a metastatic RCC of a single kidney.

المؤلفون: Podoynicin AA; MONIKI, Moscow, Russian.; First Moscow State Medical University named after I.M. Sechenov, Moscow, Russian., Mamedov EA; MONIKI, Moscow, Russian.; First Moscow State Medical University named after I.M. Sechenov, Moscow, Russian., Amosov N; MONIKI, Moscow, Russian.; First Moscow State Medical University named after I.M. Sechenov, Moscow, Russian., Garmash SV; MONIKI, Moscow, Russian.; First Moscow State Medical University named after I.M. Sechenov, Moscow, Russian., Kuznecova DA; MONIKI, Moscow, Russian.; First Moscow State Medical University named after I.M. Sechenov, Moscow, Russian., Shpot EV; MONIKI, Moscow, Russian.; First Moscow State Medical University named after I.M. Sechenov, Moscow, Russian., Mashin GA; MONIKI, Moscow, Russian.; First Moscow State Medical University named after I.M. Sechenov, Moscow, Russian., Maltsagova PS; MONIKI, Moscow, Russian.; First Moscow State Medical University named after I.M. Sechenov, Moscow, Russian.

المصدر: Urologiia (Moscow, Russia : 1999) [Urologiia] 2023 Mar (1), pp. 88-91.

نوع المنشور: Case Reports; English Abstract; Journal Article

بيانات الدورية: Publisher: Medit︠s︡ina Country of Publication: Russia (Federation) NLM ID: 100900900 Publication Model: Print Cited Medium: Print ISSN: 1728-2985 (Print) Linking ISSN: 17282985 NLM ISO Abbreviation: Urologiia Subsets: MEDLINE

مواضيع طبية MeSH: Prostatic Hyperplasia*/complications , Prostatic Hyperplasia*/surgery , Urinary Bladder Diseases* , Embolization, Therapeutic*/adverse effects , Embolization, Therapeutic*/methods, Male ; Humans ; Middle Aged ; Prostate/diagnostic imaging ; Prostate/surgery ; Prostate/blood supply ; Urinary Bladder ; Treatment Outcome ; Necrosis/complications

مستخلص: This article describes a clinical case of bladder necrosis developed after X-ray endovascular embolization of prostatic arteries of a 62-year-old patient with a verified diagnosis of BPH (benign prostatic hyperplasia). The complication resulted in the necessity of urgent surgical intervention, namely, laparotomy, cystprostatectomy and bilateral percutaneous nephrostomy. In the early postoperative period the patient had intense cutting pain in the left side of the abdomen. Examination revealed the inflow of small intestinal contents through the pelvic drainage, which was the reason for relaparotomy, abdominal cavity revision, uturing the small intestine perforation, suturing the small intestine pre-perforation, sanation and drainage of the abdominal cavity in an emergency procedure. The patient was discharged in a satisfactory condition under the supervision of a urologist by m/w on the 36th day after endovascular embolization of prostatic arteries. The eight months after discharge, the patient underwent a successful Brickers operation on creating an alternative urinary diversion route at the First Sechenov Moscow State Medical University of the Russian Federation.

المؤلفون: Rzhevskiy AS; Institute of Molecular Theranostics, Sechenov First Moscow State Medical University, 119991 Moscow, Russia.; Faculty of Computer Science, National Research University Higher School of Economics, 101000 Moscow, Russia.; Institute for Urology and Reproductive Health, Sechenov University, 119991 Moscow, Russia., Kapitannikova AY; Institute of Molecular Theranostics, Sechenov First Moscow State Medical University, 119991 Moscow, Russia., Butnaru DV; Institute for Urology and Reproductive Health, Sechenov University, 119991 Moscow, Russia., Shpot EV; Institute for Urology and Reproductive Health, Sechenov University, 119991 Moscow, Russia., Joosse SA; Department of Tumor Biology, University Medical Center Hamburg-Eppendorf, Martinistr. 52, 20246 Hamburg, Germany.; Mildred Scheel Cancer Career Center HaTriCS4, University Medical Center Hamburg-Eppendorf, Martinistr. 52, 20246 Hamburg, Germany., Zvyagin AV; Institute of Molecular Theranostics, Sechenov First Moscow State Medical University, 119991 Moscow, Russia.; MQ Photonics Centre, Macquarie University, Sydney 2109, Australia., Ebrahimi Warkiani M; Institute of Molecular Theranostics, Sechenov First Moscow State Medical University, 119991 Moscow, Russia.; School of Biomedical Engineering, University of Technology Sydney, Sydney 2007, Australia.

المصدر: Biomedicines [Biomedicines] 2022 Dec 02; Vol. 10 (12). Date of Electronic Publication: 2022 Dec 02.

نوع المنشور: Journal Article; Review

بيانات الدورية: Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101691304 Publication Model: Electronic Cited Medium: Print ISSN: 2227-9059 (Print) Linking ISSN: 22279059 NLM ISO Abbreviation: Biomedicines Subsets: PubMed not MEDLINE

مستخلص: Currently, sensitive and specific methods for the detection and prognosis of early stage PCa are lacking. To establish the diagnosis and further identify an appropriate treatment strategy, prostate specific antigen (PSA) blood test followed by tissue biopsy have to be performed. The combination of tests is justified by the lack of a highly sensitive, specific, and safe single test. Tissue biopsy is specific but invasive and may have severe side effects, and therefore is inappropriate for screening of the disease. At the same time, the PSA blood test, which is conventionally used for PCa screening, has low specificity and may be elevated in the case of noncancerous prostate tumors and inflammatory conditions, including benign prostatic hyperplasia and prostatitis. Thus, diverse techniques of liquid biopsy have been investigated to supplement or replace the existing tests of prostate cancer early diagnosis and prognostics. Here, we provide a review on the advances in diagnosis and prognostics of non-metastatic prostate cancer by means of various biomarkers extracted via liquid biopsy, including circulating tumor cells, exosomal miRNAs, and circulating DNAs.

المؤلفون: Ishenko AI; I. M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation, Moscow, Russia., Bryunin DV; I. M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation, Moscow, Russia., Shpot EV; I. M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation, Moscow, Russia., Chushkov YV; I. M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation, Moscow, Russia., Khokhlova ID; I. M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation, Moscow, Russia., Tevlina EV; I. M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation, Moscow, Russia.

المصدر: Urologiia (Moscow, Russia : 1999) [Urologiia] 2022 Sep (4), pp. 86-90.

نوع المنشور: Journal Article

بيانات الدورية: Publisher: Medit︠s︡ina Country of Publication: Russia (Federation) NLM ID: 100900900 Publication Model: Print Cited Medium: Print ISSN: 1728-2985 (Print) Linking ISSN: 17282985 NLM ISO Abbreviation: Urologiia Subsets: MEDLINE

مواضيع طبية MeSH: Endometriosis*/complications , Endometriosis*/diagnosis , Endometriosis*/surgery , Ureter*/pathology , Ureteral Diseases*/diagnosis , Ureteral Diseases*/surgery, Female ; Follow-Up Studies ; Humans ; Urologists

مستخلص: The article is focused on the problem of diagnosis and surgical treatment of infiltrative forms of endometriosis with lesions of both internal genitalia and urinary tract. A clinical observation of a young woman who underwent a robot-assisted operation on the internal genitals and organs of the urinary system is given. The article emphasizes the need for complete clinical examination in women with suspected endometriosis. The young age of patients, even the absence of bright clinic signs or absence of a long anamnesis of the disease should not exclude the possibility of severe case of endometriosis and the possibility of a combined lesions of pelvic organs. If infiltrative endometriosis is detected, the patients treatment should be carried out in a specialized hospital using modern surgical technologies.

المؤلفون: Kodzokov MA; Institute of Urology and Reproductive Health, FGAOU VO I.M. Sechenov First Moscow State Medical University, Moscow, Russia., Shpot EV; Institute of Urology and Reproductive Health, FGAOU VO I.M. Sechenov First Moscow State Medical University, Moscow, Russia., Akopyan GN; Institute of Urology and Reproductive Health, FGAOU VO I.M. Sechenov First Moscow State Medical University, Moscow, Russia., Proskura AV; Institute of Urology and Reproductive Health, FGAOU VO I.M. Sechenov First Moscow State Medical University, Moscow, Russia., Gasanov EN; Institute of Urology and Reproductive Health, FGAOU VO I.M. Sechenov First Moscow State Medical University, Moscow, Russia., Gazimiev MA; Institute of Urology and Reproductive Health, FGAOU VO I.M. Sechenov First Moscow State Medical University, Moscow, Russia.

المصدر: Urologiia (Moscow, Russia : 1999) [Urologiia] 2022 Sep (4), pp. 5-9.

نوع المنشور: Journal Article; Randomized Controlled Trial

بيانات الدورية: Publisher: Medit︠s︡ina Country of Publication: Russia (Federation) NLM ID: 100900900 Publication Model: Print Cited Medium: Print ISSN: 1728-2985 (Print) Linking ISSN: 17282985 NLM ISO Abbreviation: Urologiia Subsets: MEDLINE

مواضيع طبية MeSH: Robotics* , Urinary Incontinence*/etiology , Urinary Retention*/etiology, Humans ; Male ; Prostatectomy/adverse effects ; Prostatectomy/methods ; Urethra/surgery ; Urinary Catheters/adverse effects

مستخلص: Background: Robot-assisted radical prostatectomy (RARP) is a widely used treatment method for patients with clinically localized prostate cancer. Posterior reconstruction of urethrovesical anastomosis (UVA) is one of the techniques that provides early recovery of urinary continence after surgery. Changes in the technique of performing posterior reconstruction of UVA may contribute to further improvement of functional results.
Aim: To evaluate the functional results of early removal of the urethral catheter (after 3 days) after RARP using a modified surgical technique of performing posterior reconstruction of UVA compared with the standard catheterization time (7 days after surgery).
Materials and Methods: Patients who underwent RARP were randomly assigned to early catheter removal (3 days after surgery, main group, n=15) and standard catheterization time (7 days after surgery, control group, n=15). RARP was performed using the Da Vinci Si system. The primary end point was the rate of spontaneous voiding after catheter removal. Secondary endpoints were the incidence of urine leakage into the paravesical tissue during retrograde cystography, as well as complications according to the Clavien-Dindo system. The frequency and severity of stress urinary incontinence after catheter removal was assessed using the International Consultation on Incontinence Questionnaire - Urinary Incontinence Short Form (ICIQ-UI SF).
Results: There were no significant differences between groups in terms of baseline and perioperative parameters. After removal of the catheter, no acute urinary retention was observed in both groups. Also, there were no cases of urine leakage into the paravesical tissue during retrograde cystography. The ICIQ-UI SF questionnaire showed no significant differences between the groups at 1, 3, 6, and 12 months after surgery.
Conclusions: The method of modified posterior reconstruction allows to remove the urethral catheter 3 days after RARP. Early removal of the urethral catheter did not adversely affect the early recovery of urinary continence, the quality of UVA, and did not increase the incidence of acute urinary retention. Further studies with longer follow-up periods are needed.

المؤلفون: Rzhevskiy AS; Institute of Molecular Theranostics, Sechenov First Moscow State Medical University, 119991 Moscow, Russia.; Institute for Urology and Reproductive Health, Sechenov University, 119146 Moscow, Russia., Kapitannikova AY; Institute of Molecular Theranostics, Sechenov First Moscow State Medical University, 119991 Moscow, Russia., Vasilescu SA; School of Biomedical Engineering, University of Technology Sydney, Sydney 2007, Australia., Karashaeva TA; Institute of Molecular Theranostics, Sechenov First Moscow State Medical University, 119991 Moscow, Russia., Razavi Bazaz S; School of Biomedical Engineering, University of Technology Sydney, Sydney 2007, Australia., Taratkin MS; Institute for Urology and Reproductive Health, Sechenov University, 119146 Moscow, Russia., Enikeev DV; Institute for Urology and Reproductive Health, Sechenov University, 119146 Moscow, Russia.; Department of Urology, Medical University of Vienna, 1090 Vienna, Austria., Lekarev VY; Institute for Urology and Reproductive Health, Sechenov University, 119146 Moscow, Russia., Shpot EV; Institute for Urology and Reproductive Health, Sechenov University, 119146 Moscow, Russia., Butnaru DV; Institute for Urology and Reproductive Health, Sechenov University, 119146 Moscow, Russia., Deyev SM; Institute of Molecular Theranostics, Sechenov First Moscow State Medical University, 119991 Moscow, Russia.; Molecular Immunology Laboratory, Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia.; Research Centrum for Oncotheranostics, Research School of Chemistry and Applied Biomedical Sciences, Tomsk Polytechnic University, 634050 Tomsk, Russia.; Bio-Nanophotonic Laboratory, Institute of Engineering Physics for Biomedicine (PhysBio), National Research Nuclear University 'MEPhI', 115409 Moscow, Russia., Thiery JP; Institute of Molecular Theranostics, Sechenov First Moscow State Medical University, 119991 Moscow, Russia.; Guangzhou Laboratory, Bioisland, Guangzhou 510320, China., Zvyagin AV; Institute of Molecular Theranostics, Sechenov First Moscow State Medical University, 119991 Moscow, Russia.; Molecular Immunology Laboratory, Shemyakin & Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia.; Bio-Nanophotonic Laboratory, Institute of Engineering Physics for Biomedicine (PhysBio), National Research Nuclear University 'MEPhI', 115409 Moscow, Russia.; MQ Photonics Centre, Macquarie University, Sydney 2109, Australia., Ebrahimi Warkiani M; Institute of Molecular Theranostics, Sechenov First Moscow State Medical University, 119991 Moscow, Russia.; School of Biomedical Engineering, University of Technology Sydney, Sydney 2007, Australia.

المصدر: Cancers [Cancers (Basel)] 2022 Jul 11; Vol. 14 (14). Date of Electronic Publication: 2022 Jul 11.

نوع المنشور: Journal Article

بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101526829 Publication Model: Electronic Cited Medium: Print ISSN: 2072-6694 (Print) Linking ISSN: 20726694 NLM ISO Abbreviation: Cancers (Basel) Subsets: PubMed not MEDLINE

مستخلص: Prostate cancer (PCa) diagnosis is primarily based on prostate-specific antigen (PSA) testing and prostate tissue biopsies. However, PSA testing has relatively low specificity, while tissue biopsies are highly invasive and have relatively low sensitivity at early stages of PCa. As an alternative, we developed a technique of liquid biopsy, based on isolation of circulating tumor cells (CTCs) from seminal fluid (SF). The recovery of PCa cells from SF was demonstrated using PCa cell lines, achieving an efficiency and throughput as high as 89% (±3.8%) and 1.7 mL min ^-1 , respectively, while 99% (±0.7%) of sperm cells were disposed of. The introduced approach was further tested in a clinical setting by collecting and processing SF samples of PCa patients. The yield of isolated CTCs measured as high as 613 cells per SF sample in comparison with that of 6 cells from SF of healthy donors, holding significant promise for PCa diagnosis. The correlation analysis of the isolated CTC numbers with the standard prognostic parameters such as Gleason score and PSA serum level showed correlation coefficient values at 0.40 and 0.73, respectively. Taken together, our results show promise in the developed liquid biopsy technique to augment the existing diagnosis and prognosis of PCa.

المؤلفون: Mashin GA; Institute for Urology and Human Reproductive Health, Sechenov University, Moscow, Russia., Kozlov VV; Institute for Urology and Human Reproductive Health, Sechenov University, Moscow, Russia., Chinenov DV; Institute for Urology and Human Reproductive Health, Sechenov University, Moscow, Russia., Chernov YN; Institute for Urology and Human Reproductive Health, Sechenov University, Moscow, Russia., Proskura AV; Institute for Urology and Human Reproductive Health, Sechenov University, Moscow, Russia., Korolev DO; Institute for Urology and Human Reproductive Health, Sechenov University, Moscow, Russia., Tsarichenko DG; Institute for Urology and Human Reproductive Health, Sechenov University, Moscow, Russia., Rapoport LM; Institute for Urology and Human Reproductive Health, Sechenov University, Moscow, Russia., Shpot EV; Institute for Urology and Human Reproductive Health, Sechenov University, Moscow, Russia.

المصدر: Urologia [Urologia] 2022 May; Vol. 89 (2), pp. 179-184. Date of Electronic Publication: 2021 Aug 02.

نوع المنشور: Journal Article

بيانات الدورية: Publisher: Sage Publications Country of Publication: United States NLM ID: 0417372 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1724-6075 (Electronic) Linking ISSN: 03915603 NLM ISO Abbreviation: Urologia Subsets: MEDLINE

مواضيع طبية MeSH: Kidney Neoplasms*/pathology , Kidney Neoplasms*/surgery , Nephrectomy*/methods, Female ; Humans ; Kidney/surgery ; Male ; Postoperative Complications/epidemiology ; Postoperative Complications/etiology ; Probability ; Retrospective Studies

مستخلص: Aim: The purpose of the study is the development and evaluation of the informativeness of the author's 3D nephrometric score application to predict the probability of intraoperative and postoperative complications in kidney operations.
Material and Methods: The study includes 264 patients who underwent surgical treatment of renal tumors, before that CT and 3D modeling were carried out. All patients underwent an analysis of the surgical intervention complexity on the C-index, PADUA, R.E.N.A.L., and developed 3D nephrometric score. To determine the set of variables that allow to classify patients, the method of discriminant analysis was used to predict the nature, volume of blood loss, duration of ischemia, and the number of complications. The sensitivity and specificity of the predictors were estimated with the help of ROC analysis.
Results: Indicators have been established to classify patients according to the probability of complications, the amount of blood loss and the duration of ischemia during surgery for kidney cancer. We have created linear models that predict the development of bleeding during surgery, the volume of blood loss of more than 200 ml and the duration of ischemia more than 20 min, as well as the likelihood of complications using discriminant functions. The proposed author's nephrometric score exceeds the capabilities of C-index, PADUA, R.E.N.A.L in many ways in blood loss and time of ischemia predicting, which allows us to recommend it for the assessment of resectability in kidney operations.

المؤلفون: Anoshkin KI; Research Centre for Medical Genetics, Moscow, Russia., Karandasheva KO; Research Centre for Medical Genetics, Moscow, Russia., Goryacheva KM; I.M. Sechenov First Moscow State Medical University, Moscow, Russia., Pyankov DV; Genomed Ltd., Moscow, Russia., Koshkin PA; Genomed Ltd., Moscow, Russia., Pavlova TV; N.N. Burdenko Main Military and Clinical Hospital, Moscow, Russia., Bobin AN; N.N. Burdenko Main Military and Clinical Hospital, Moscow, Russia., Shpot EV; I.M. Sechenov First Moscow State Medical University, Moscow, Russia., Chernov YN; I.M. Sechenov First Moscow State Medical University, Moscow, Russia., Vinarov AZ; I.M. Sechenov First Moscow State Medical University, Moscow, Russia., Zaletaev DV; I.M. Sechenov First Moscow State Medical University, Moscow, Russia., Kutsev SI; Research Centre for Medical Genetics, Moscow, Russia., Strelnikov VV; Research Centre for Medical Genetics, Moscow, Russia.

المصدر: Frontiers in oncology [Front Oncol] 2020 Aug 19; Vol. 10, pp. 1653. Date of Electronic Publication: 2020 Aug 19 (Print Publication: 2020).

نوع المنشور: Case Reports

بيانات الدورية: Publisher: Frontiers Research Foundation] Country of Publication: Switzerland NLM ID: 101568867 Publication Model: eCollection Cited Medium: Print ISSN: 2234-943X (Print) Linking ISSN: 2234943X NLM ISO Abbreviation: Front Oncol Subsets: PubMed not MEDLINE

مستخلص: We present the genetic profile of kidney giant leiomyosarcoma characterized by sequencing of 409 cancer related genes and chromosomal microarray analysis. Renal leiomyosarcomas are extremely rare neoplasms with aggressive behavior and poor survival prognosis. Most frequent somatic events in leiomyosarcomas are mutations in the TP53, RB1, ATRX, and PTEN genes, chromosomal instability (CIN) and chromoanagenesis. 67-year-old woman presented with a right kidney completely replaced by tumor. Immunohistochemical reaction on surgical material was positive to desmin and smooth muscle actin. Molecular genetic analysis revealed that tumor harbored monosomy of chromosomes 3 and 11, gain of Xp (ATRX) arm and three chromoanasynthesis regions (6q21-q27, 7p22.3-p12.1, and 12q13.11-q21.2), with MDM2 and CDK4 oncogenes copy number gains, whereas no copy number variations (CNVs) or tumor specific single nucleotide variants (SNVs) in TP53, RB1, and PTEN genes were present. We hypothesize that chromoanasynthesis in 12q13.11-q21.2 could be a trigger of observed CIN in this tumor.
(Copyright © 2020 Anoshkin, Karandasheva, Goryacheva, Pyankov, Koshkin, Pavlova, Bobin, Shpot, Chernov, Vinarov, Zaletaev, Kutsev and Strelnikov.)