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1دورية أكاديمية
المؤلفون: Denise Chan, Phillip R. Bennett, Yun S. Lee, Samit Kundu, T. G. Teoh, Malko Adan, Saqa Ahmed, Richard G. Brown, Anna L. David, Holly V. Lewis, Belen Gimeno-Molina, Jane E. Norman, Sarah J. Stock, Vasso Terzidou, Pascale Kropf, Marina Botto, David A. MacIntyre, Lynne Sykes
المصدر: Nature Communications, Vol 13, Iss 1, Pp 1-15 (2022)
مصطلحات موضوعية: Science
الوصف: Gaining mechanistic insight into the microbiological and immunological factors that are associated with spontaneous preterm birth is important for the development of prevention strategies. Here authors show that the complement system in conjunction with specific vaginal microbial and associated immunological changes are contributing to this condition.
وصف الملف: electronic resource
Relation: https://doaj.org/toc/2041-1723
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2دورية أكاديمية
المؤلفون: Pamela Pruski, Gonçalo D. S. Correia, Holly V. Lewis, Katia Capuccini, Paolo Inglese, Denise Chan, Richard G. Brown, Lindsay Kindinger, Yun S. Lee, Ann Smith, Julian Marchesi, Julie A. K. McDonald, Simon Cameron, Kate Alexander-Hardiman, Anna L. David, Sarah J. Stock, Jane E. Norman, Vasso Terzidou, T. G. Teoh, Lynne Sykes, Phillip R. Bennett, Zoltan Takats, David A. MacIntyre
المصدر: Nature Communications, Vol 12, Iss 1, Pp 1-14 (2021)
مصطلحات موضوعية: Science
الوصف: Here, the authors apply DESI-MS, a sample preparation-free, direct on-swab mass spectrometry analytical tool, to profile the cervicovaginal metabolome of two independent cohorts of pregnant women and, combined with matched metataxonomic and immuno-profiling data, show that DESI-MS predicts vaginal microbiota composition and local inflammatory status associated with preterm birth and clinical interventions used during pregnancy.
وصف الملف: electronic resource
Relation: https://doaj.org/toc/2041-1723
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المؤلفون: L Kindinger, Julie A. K. McDonald, Vasso Terzidou, Phillip R. Bennett, Katia Capuccini, Denise Chan, Holly V. Lewis, Anna L. David, Lynne Sykes, David A. MacIntyre, Zoltan Takats, Jane E. Norman, Paolo Inglese, Sarah J. Stock, Kate Alexander-Hardiman, Yun S. Lee, Julian Marchesi, Richard G. Brown, Pamela Pruski, Simon J S Cameron, T. G. Teoh, Ann Smith, Gonçalo D S Correia
المساهمون: Medical Research Council (MRC), Genesis Research Trust, Imperial College Healthcare NHS Trust- BRC Funding, Commission of the European Communities, March of Dimes
المصدر: Pruski, P, dos Santos Correia, G, Lewis, H, Capuccini, K, Inglese, P, Chan, D, Brown, R, Kindinger, L, Lee, Y, Smith, A, Marchesi, J, McDonald, J, Cameron, S, Alexander-Hardiman, K, David, A, Stock, S J E, Norman, J, Terzidou, V, Teoh, TG, Sykes, L, Bennett, P, Takats, Z & MacIntyre, D 2021, ' Direct on-swab metabolic profiling of vaginal microbiome host interactions during pregnancy and preterm birth ', Nature Communications, vol. 12, 5967 . https://doi.org/10.1038/s41467-021-26215-w
Nature Communications, Vol 12, Iss 1, Pp 1-14 (2021)
Pruski, P, Correia, G D S, Lewis, H V, Capuccini, K, Inglese, P, Chan, D, Brown, R G, Kindinger, L, Lee, Y S, Smith, A, Marchesi, J, McDonald, J A K, Cameron, S, Alexander-Hardiman, K, David, A L, Stock, S J, Norman, J E, Terzidou, V, Teoh, T G, Sykes, L, Bennett, P R, Takats, Z & MacIntyre, D A 2021, ' Direct on-swab metabolic profiling of vaginal microbiome host interactions during pregnancy and preterm birth ', Nature Communications, vol. 12, no. 1, 5967, pp. 5967 . https://doi.org/10.1038/s41467-021-26215-w
Nature Communicationsمصطلحات موضوعية: medicine.medical_treatment, IONIZATION MASS-SPECTROMETRY, Desorption electrospray ionization mass spectrometry, General Physics and Astronomy, Physiology, Cervix Uteri, Immune profiling, Pregnancy, Medicine, Prospective Studies, Cause of death, Cerclage, Cervical, Multidisciplinary, Microbiota, WOMEN, Multidisciplinary Sciences, BACTERIA, Vagina, Vaginal microbiome, Metabolome, Science & Technology - Other Topics, SECRETION, Premature Birth, Female, Infant, Premature, Adult, Spectrometry, Mass, Electrospray Ionization, Science, INTERLEUKIN-1-ALPHA, Predictive markers, Article, General Biochemistry, Genetics and Molecular Biology, MANNOSE-BINDING LECTIN, Humans, Cervical cerclage, Science & Technology, business.industry, GENITAL-TRACT, Infectious-disease diagnostics, Infant, Newborn, PREVENT, General Chemistry, Translational research, medicine.disease, Immunity, Innate, Rapid assessment, Risk factors, AMBIENT IONIZATION, ENDOTOXIN, business
الوصف: The pregnancy vaginal microbiome contributes to risk of preterm birth, the primary cause of death in children under 5 years of age. Here we describe direct on-swab metabolic profiling by Desorption Electrospray Ionization Mass Spectrometry (DESI-MS) for sample preparation-free characterisation of the cervicovaginal metabolome in two independent pregnancy cohorts (VMET, n = 160; 455 swabs; VMET II, n = 205; 573 swabs). By integrating metataxonomics and immune profiling data from matched samples, we show that specific metabolome signatures can be used to robustly predict simultaneously both the composition of the vaginal microbiome and host inflammatory status. In these patients, vaginal microbiota instability and innate immune activation, as predicted using DESI-MS, associated with preterm birth, including in women receiving cervical cerclage for preterm birth prevention. These findings highlight direct on-swab metabolic profiling by DESI-MS as an innovative approach for preterm birth risk stratification through rapid assessment of vaginal microbiota-host dynamics.
Here, the authors apply DESI-MS, a sample preparation-free, direct on-swab mass spectrometry analytical tool, to profile the cervicovaginal metabolome of two independent cohorts of pregnant women and, combined with matched metataxonomic and immuno-profiling data, show that DESI-MS predicts vaginal microbiota composition and local inflammatory status associated with preterm birth and clinical interventions used during pregnancy.وصف الملف: application/pdf; PDF
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المؤلفون: Denise, Chan, Phillip R, Bennett, Yun S, Lee, Samit, Kundu, T G, Teoh, Malko, Adan, Saqa, Ahmed, Richard G, Brown, Anna L, David, Holly V, Lewis, Belen, Gimeno-Molina, Jane E, Norman, Sarah J, Stock, Vasso, Terzidou, Pascale, Kropf, Marina, Botto, David A, MacIntyre, Lynne, Sykes
المصدر: Nature communications. 13(1)
مصطلحات موضوعية: Adult, Microbiota, Infant, Newborn, Cervix Uteri, Adaptive Immunity, Immunity, Innate, Lactobacillus, Pregnancy, Case-Control Studies, Vagina, Humans, Premature Birth, Female, Prospective Studies
الوصف: There has been a surge in studies implicating a role of vaginal microbiota in spontaneous preterm birth (sPTB), but most are associative without mechanistic insight. Here we show a comprehensive approach to understand the causative factors of preterm birth, based on the integration of longitudinal vaginal microbiota and cervicovaginal fluid (CVF) immunophenotype data collected from 133 women at high-risk of sPTB. We show that vaginal depletion of Lactobacillus species and high bacterial diversity leads to increased mannose binding lectin (MBL), IgM, IgG, C3b, C5, IL-8, IL-6 and IL-1β and to increased risk of sPTB. Cervical shortening, which often precedes preterm birth, is associated with Lactobacillus iners and elevated levels of IgM, C3b, C5, C5a and IL-6. These data demonstrate a role for the complement system in microbial-driven sPTB and provide a scientific rationale for the development of live biotherapeutics and complement therapeutics to prevent sPTB.
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المؤلفون: Liona C. Poon, Kim Munnery, Rebecca Playle, Jayanta Banerjee, Tom Bourne, Julia Townson, Alison Wright, Edward Mullins, Caroline J. Shaw, T. G. Teoh, Nigel Kirby, Mandish Dhanjal, Christoph Lees
المساهمون: Medical Research Council (MRC)
المصدر: BMJ Open
BMJ Open, Vol 11, Iss 1 (2021)مصطلحات موضوعية: Research design, lcsh:Medicine, infectious diseases, Global Health, Miscarriage, 0302 clinical medicine, Pregnancy, Pandemic, Global health, Registries, 030212 general & internal medicine, Pregnancy Complications, Infectious, obstetrics, 030219 obstetrics & reproductive medicine, public health, Pregnancy Outcome, General Medicine, Natural history, Maternal Mortality, Research Design, Premature Birth, Female, Life Sciences & Biomedicine, medicine.medical_specialty, neonatology, 1117 Public Health and Health Services, 03 medical and health sciences, Medicine, General & Internal, General & Internal Medicine, medicine, Humans, Science & Technology, SARS-CoV-2, business.industry, Public health, lcsh:R, Infant, Newborn, COVID-19, 1103 Clinical Sciences, medicine.disease, Infectious Disease Transmission, Vertical, United Kingdom, Abortion, Spontaneous, Emergency medicine, Observational study, business, 1199 Other Medical and Health Sciences
الوصف: IntroductionPrevious novel COVID-19 pandemics, SARS and middle east respiratory syndrome observed an association of infection in pregnancy with preterm delivery, stillbirth and increased maternal mortality. COVID-19, caused by SARS-CoV-2 infection, is the largest pandemic in living memory.Rapid accrual of robust case data on women in pregnancy and their babies affected by suspected COVID-19 or confirmed SARS-CoV-2 infection will inform clinical management and preventative strategies in the current pandemic and future outbreaks.Methods and analysisThe pregnancy and neonatal outcomes in COVID-19 (PAN-COVID) registry are an observational study collecting focused data on outcomes of pregnant mothers who have had suspected COVID-19 in pregnancy or confirmed SARS-CoV-2 infection and their neonates via a web-portal. Among the women recruited to the PAN-COVID registry, the study will evaluate the incidence of: (1) miscarriage and pregnancy loss, (2) fetal growth restriction and stillbirth, (3) preterm delivery, (4) vertical transmission (suspected or confirmed) and early onset neonatal SARS-CoV-2 infection.Data will be centre based and collected on individual women and their babies. Verbal consent will be obtained, to reduce face-to-face contact in the pandemic while allowing identifiable data collection for linkage. Statistical analysis of the data will be carried out on a pseudonymised data set by the study statistician. Regular reports will be distributed to collaborators on the study research questions.Ethics and disseminationThis study has received research ethics approval in the UK. For international centres, evidence of appropriate local approval will be required to participate, prior to entry of data to the database. The reports will be published regularly. The outputs of the study will be regularly disseminated to participants and collaborators on the study website (https://pan-covid.org) and social media channels as well as dissemination to scientific meetings and journals.Study registration numberISRCTN68026880.
وصف الملف: Electronic
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المؤلفون: Holly V. Lewis, T. G. Teoh, Sara Zarasvand, Lynne Sykes, Katherine Mountain, Karen Joash, Erna Bayar, Sabrina Das, Malko Adan, Phillip R. Bennett
المساهمون: March of Dimes
المصدر: BMJ Open Quality
e001049
BMJ Open Quality, Vol 9, Iss 4 (2020)مصطلحات موضوعية: medicine.medical_specialty, Medicine (General), Quality management, Leadership and Management, medicine.medical_treatment, State Medicine, 03 medical and health sciences, 0302 clinical medicine, R5-920, Obstetrics and gynaecology, Pregnancy, Epidemiology, Pandemic, Health care, medicine, Humans, Cervical cerclage, 030212 general & internal medicine, Original Research, obstetrics and gynecology, Remote Consultation, 030219 obstetrics & reproductive medicine, business.industry, maternal health services, Health Policy, Infant, Newborn, Public Health, Environmental and Occupational Health, COVID-19, medicine.disease, Quality Improvement, United Kingdom, Emergency medicine, Premature Birth, Female, business
الوصف: BackgroundPreterm birth (PTB) occurs in 8% of births in the UK. At Imperial College Healthcare NHS Trust, our PTB prevention clinic manages the care of approximately 1000 women/year. Women referred to the clinic are seen from 12 weeks of pregnancy with subsequent appointments every 2–4 weeks to measure cervical length (CL) using transvaginal ultrasound (TVUS). Women with a history of cervical weakness or short cervix on TVUS are offered a cervical cerclage.Local problemDuring the COVID-19 outbreak, pregnant women were strongly advised to avoid social mixing and public transport. The National Health Service had to rapidly adopt remote consultation and redesign clinical pathways in order to reduce transmission, exposure and spread among women at high risk of PTB.MethodsWe focused on Specific, Measurable, Achievable, Realistic and Timebound aims and used a driver diagram to visualise our changes. We used a series of Plan Do Study Act cycles to evaluate and adapt change ideas through the UK’s national lockdown during the COVID-19 pandemic between 23 March and 29 May 2020.ResultsWe reduced the number of face-to-face appointments by 54%. This was achieved by increasing remote telephone consultations from 0% to 64%, and by reducing the intensity of surveillance. The rate of regional anaesthetic was increased from 53% to 95% for cerclage placement in order to minimise the number of aerosol-generating procedures. Patient and staff satisfaction responses to these changes were used to tailor practices. No women tested positive for COVID-19 during the study period.ConclusionsBy using quality improvement methodology, we were able to safely and rapidly implement a new care pathway for women at high risk of PTB which was acceptable to patients and staff, and effective in reducing exposure of COVID-19.
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المؤلفون: Lynne Sykes, L Kindinger, Alice Garrett, T. G. Teoh, Phillip R. Bennett, Reem Binkhamis, Vasso Terzidou, Joanna R. Cook, Sung Hye Kim, David A. MacIntyre
المساهمون: Medical Research Council (MRC), Imperial College Healthcare NHS Trust- BRC Funding, Action Medical Research, Seattle ChildrensHospital Research Foundation, Genesis Research Trust
المصدر: Scientific Reports
Scientific Reports, Vol 9, Iss 1, Pp 1-9 (2019)مصطلحات موضوعية: 0301 basic medicine, EXPRESSION, Adult, medicine.medical_specialty, BIRTH, lcsh:Medicine, Cervix Uteri, Asymptomatic, Article, Cohort Studies, 03 medical and health sciences, 0302 clinical medicine, Pregnancy, medicine, LENGTH, Humans, lcsh:Science, Preterm delivery, RISK, Multidisciplinary, Science & Technology, Obstetrics, business.industry, lcsh:R, PROLIFERATION, medicine.disease, Cervical shortening, 3. Good health, Multidisciplinary Sciences, body regions, First trimester, Circulating MicroRNA, MicroRNAs, Pregnancy Trimester, First, 030104 developmental biology, ROC Curve, Area Under Curve, embryonic structures, Gestation, Science & Technology - Other Topics, Premature Birth, lcsh:Q, Female, medicine.symptom, business, 030217 neurology & neurosurgery, Biomarkers, Cohort study
الوصف: Preterm birth (PTB) is the leading cause of infant death and disability worldwide. The onset of preterm uterine contractions is preceded by asymptomatic cervical remodelling and ripening, which can be seen on trans-vaginal ultrasound as cervical shortening. This study aimed to identify plasma miRNA biomarkers that predict preterm birth and/or cervical shortening. We collected serial plasma samples from pregnant women prospectively from 12 to 22 weeks gestation. The nCounter miRNA assay was used to identify differentially expressed miRNAs associated with spontaneous PTB and/or cervical shortening (n = 16 term no short, n = 13 preterm, n = 24 short). Predictive values of the miRNA biomarkers were confirmed in an independent validation cohort consisting of 96 women who delivered at term, 14 preterm and 21 early cervical shortening at P
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8دورية أكاديمية
المؤلفون: Lindsay M Kindinger, Maria Kyrgiou, David A MacIntyre, Stefano Cacciatore, Angela Yulia, Joanna Cook, Vasso Terzidou, T G Teoh, Phillip R Bennett
المصدر: Public Library of Science, PLOS ONE. 11(11):1-15
الوصف: Women with a history of excisional treatment (conization) for cervical intra-epithelial neoplasia (CIN) are at increased risk of preterm birth, perinatal morbidity and mortality in subsequent pregnancy. We aimed to develop a screening model to effectively differentiate pregnancies post-conization into low- and high-risk for preterm birth, and to evaluate the impact of suture material on the efficacy of ultrasound indicated cervical cerclage. We analysed longitudinal cervical length (CL) data from 725 pregnant women post-conization attending preterm surveillance clinics at three London university Hospitals over a ten year period (2004–2014). Rates of preterm birth 37weeks. Our triage model enables timely discharge of low risk women, eliminating 36% of unnecessary follow-up CL scans. We demonstrate that preterm birth in women post-conization may be reduced by targeted cervical cerclage. Cerclage efficacy is however suture material-dependant: monofilament is preferable to braided suture. The introduction of triage prediction models has the potential to reduce the number of unnecessary CL scan for women at low risk of preterm birth.
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المؤلفون: Phillip R. Bennett, Kacie Thomson, Yun S. Lee, David A. MacIntyre, Lynne Sykes, Emily J. Boyce, Zahirrah B. M. Rasheed, T. G. Teoh
المصدر: Immunology. 146:630-644
مصطلحات موضوعية: medicine.medical_specialty, Necrosis, medicine.medical_treatment, Interleukin-1beta, Myocytes, Smooth Muscle, Immunology, nuclear factor-κB, Inflammation, Pregnancy, Fetal membrane, Sulfasalazine, Internal medicine, 1114 Paediatrics And Reproductive Medicine, Humans, Immunology and Allergy, Medicine, Amnion, Cells, Cultured, Nuclear Factor κ B (NF-κB), Myocytes, Fetus, Amniocytes, business.industry, Interleukins, Sulfasalazine (SASP), NF-kappa B, Interleukin, Epithelial Cells, Original Articles, NFKB1, Transcription Factor AP-1, Activator of protein -1 (AP-1), Endocrinology, Cytokine, 1107 Immunology, Cyclooxygenase 2, Cytokines, Female, Inflammation Mediators, medicine.symptom, business, activator of protein-1, medicine.drug
الوصف: Preterm birth occurs in 10% of pregnancies and is a major cause of neonatal morbidity and mortality. The majority of cases of early preterm labour are associated with infection/inflammation, which places the fetal central nervous system at risk. Targeting immune activation is therefore an appealing therapeutic strategy for the prevention of preterm labour and neonatal brain injury. The expression of many labour‐associated and inflammatory‐response genes is controlled by the transcription factors nuclear factor‐κB (NF‐κB) and activator protein‐1 (AP‐1), which makes them therapeutic targets of interest. Sulfasalazine (SASP) has been shown to inhibit NF‐κB and reduce lipopolysaccharide‐induced cytokine concentrations in fetal membrane explants and reduce the rate of Escherichia coli‐induced preterm labour in mice. Its effects upon AP‐1 in the context of pregnancy are unknown. In this study the effect of SASP on interleukin‐1β (IL‐1β) ‐induced NF‐κB and AP‐1 activity, cytokine production and cyclo‐oxygenase‐2 (COX‐2) expression was examined in amniocytes and myocytes. A supra‐therapeutic concentration (5 mm) was required to inhibit IL‐1β‐induced NF‐κB (P
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المؤلفون: Julian R. Marchesi, T. G. Teoh, Jeremy K. Nicholson, L Kindinger, David A. MacIntyre, Yun S. Lee, Phillip R. Bennett, Stefano Cacciatore, Elaine Holmes, Ann Smith
المساهمون: Medical Research Council (MRC), Imperial College Healthcare NHS Trust- BRC Funding
المصدر: Microbiome
مصطلحات موضوعية: 0301 basic medicine, Pregnancy, High-Risk, Cervix Uteri, PLACEBO-CONTROLLED TRIAL, DOUBLE-BLIND, 0302 clinical medicine, 1108 Medical Microbiology, Pregnancy, RNA, Ribosomal, 16S, Prospective Studies, Progesterone, 030219 obstetrics & reproductive medicine, FLORA, Obstetrics, Microbiota, Pregnancy Outcome, HUMAN MYOMETRIAL CELLS, 3. Good health, Cervical Length Measurement, medicine.anatomical_structure, Premature birth, BACTERIAL VAGINOSIS, Vagina, Premature Birth, Term Birth, Gestation, Female, Bacterial vaginosis, Life Sciences & Biomedicine, PREGNANT-WOMEN, 0605 Microbiology, Adult, Microbiology (medical), medicine.medical_specialty, HORMONAL CONTRACEPTION, Biology, Vaginal microbiome, Microbiology, DELIVERY, Young Adult, 03 medical and health sciences, medicine, Lactobacillus iners, Humans, Gynecology, Science & Technology, 0602 Ecology, GENITAL-TRACT, Research, Preterm birth, Pessaries, biology.organism_classification, medicine.disease, Bacterial Load, Lactobacillus, Cross-Sectional Studies, 030104 developmental biology, Dysbiosis, Cervical length
الوصف: Background Preterm birth is the primary cause of infant death worldwide. A short cervix in the second trimester of pregnancy is a risk factor for preterm birth. In specific patient cohorts, vaginal progesterone reduces this risk. Using 16S rRNA gene sequencing, we undertook a prospective study in women at risk of preterm birth (n = 161) to assess (1) the relationship between vaginal microbiota and cervical length in the second trimester and preterm birth risk and (2) the impact of vaginal progesterone on vaginal bacterial communities in women with a short cervix. Results Lactobacillus iners dominance at 16 weeks of gestation was significantly associated with both a short cervix
وصف الملف: application/pdf
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