المؤلفون: Jiang, Yi, Li, Jun-Jie, Mu, Ya-Wei, Jiang, Han-Yang, Wei, Zi-Xuan, Xiao, Zi-Yao, Zhao, Jing-Jing, Chen, Xian-Hua^{Aff1, IDs1264002200473y_cor8}

المصدر: Neurotoxicity Research: Neurodegeneration, Neuroregeneration, Neurotrophic Action, and Neuroprotection. 40(2):365-372

المؤلفون: Fan, Zhen, Sun, Bing, Lang, Li-qin, Hu, Jie^Aff1, Hameed, N. U. Farrukh, Wei, Zi-xuan, Zhuang, Qi-yuan, Cai, Jia-jun, Liu, Feng-tao, Mao, Yi-ting, Feng, Rui^Aff1, Pan, Li

المصدر: Neurological Sciences. 42(6):2353-2361

المؤلفون: Zhou, Nan, Wei, Zi Xuan, Qi, Zeng Xin^Aff1

المصدر: BMC Neuroscience. 20(1)

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المؤلفون: Lin, Hui, Shen, Yong-Chun, Long, Hong-Yu, Wang, Hao, Luo, Ze-Yu, Wei, Zi-Xuan, Hu, Shi-Qi, Wen, Fu-Qiang

مصطلحات موضوعية: Original Article

الوصف: It is reported that osteopontin has shown promising diagnostic value for malignant pleural mesothelioma (MPM), this meta-analysis aimed to establish the overall diagnostic accuracy of the osteopontin measurement for diagnosing MPM. Based on a systematic review of English language studies, the sensitivity, specificity and other measures of accuracy of osteopontin in the diagnosis of MPM were pooled using random-effects model. Summary receiver operating characteristic curves were used to summarize overall test performance. Seven publications met our inclusion criteria, the pooled sensitivity was 0.57 (95%CI: 0.52-0.61), specificity was 0.81, 95%CI: 0.79-0.84). The PLR was 3.78 (95%CI: 2.23-6.41), the NLR was 0.51 (95%CI: 0.38-0.67) and the DOR was 9.04 (95%CI: 5.28-15.48), the area under the summary receiver operating characteristic curve was 0.80. Our data suggest that osteopontin is likely to be a useful diagnostic marker for MPM, considering for the limited studies and patients included, larger studies are needed to confirm these findings.

URL الوصول: https://explore.openaire.eu/search/publication?articleId=pmid________::d3dbf68b61d783e56fac3c6ddf5b85c4
https://europepmc.org/articles/PMC4073746/

المؤلفون: Zhang C; State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510006, China., Wei ZX; State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510006, China., Wang M; State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510006, China., Chen YS; State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510006, China., He ZY; State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510006, China.

المصدر: Yi chuan = Hereditas [Yi Chuan] 2022 Jul 20; Vol. 44 (7), pp. 581-590.

نوع المنشور: Journal Article

بيانات الدورية: Publisher: Ke xue chu ban she Country of Publication: China NLM ID: 9436478 Publication Model: Print Cited Medium: Print ISSN: 0253-9772 (Print) Linking ISSN: 02539772 NLM ISO Abbreviation: Yi Chuan Subsets: MEDLINE

مواضيع طبية MeSH: Melanoma*/genetics , Melanoma*/metabolism , Receptor, Melanocortin, Type 1*/genetics , Receptor, Melanocortin, Type 1*/metabolism, Animals ; CRISPR-Cas Systems ; Humans ; Mammals/metabolism ; Melanins/genetics ; Monophenol Monooxygenase/metabolism ; Swine

مستخلص: MC1R (melanocortin 1 receptor) encodes the melanocortin-1 receptor, which can activate intracellular cAMP synthesis under the stimulation of the α-melanocyte stimulating hormone (α-MSH) ligand. Increased cAMP then activates the protein kinase A (PKA) pathway, resulting in the up-regulation of the expression of the microphthalmia-associated transcription factor (MITF) which is a critical regulatory factor of melanin synthesis, and tyrosinase (TYR), the rate-limiting enzyme of melanin synthesis tyrosinase (TYR), and ultimately affects production of eumelanin and pheomelanin, and the coat color phenotype of mammalian species. Previous reports have indicated that the mutation A243T in the transmembrane domain 6 (TM6) of MC1R protein might disrupt the function of MC1R, contributing to the red phenotype in Duroc pig. However, functional analysis of the A243T mutation in MC1R has not yet been carried out. In this study, we attempted to used single-stranded oligo-deoxyribonucleotides (ssODN) as donor templates to introduce the c.727G>A (A243T) mutation into MC1R in human melanoma cell line SK-MEL-2 by CRISPR/Cas9 to analyze its effects on MC1R functions. We found the occurrence of ssODN recombination reached to 10%. Unfortunately, Sanger sequencing MC1R in six single-cell clones revealed that none carried the c.727G>A mutation, but all carried undesired mutations surrounding the target site. Cells transfected with CRISPR/Cas9 plasmids and ssODN presented significantly attenuated cAMP activation, and down-regulated MITF and TYR expression, indicating that the editing MC1R could affect the melanin synthesis function in cells. This study provides a basis for further investigation the mechanism of MC1R mutation on animal coat color.

المؤلفون: Lin H; Department of Rheumatology and Immunology, West China Hospital of Sichuan University Chengdu, China., Shen YC; Department of Pulmonary Diseases, State Key Laboratory of Biotherapy of China and Department of Respiratory Medicine, West China Hospital of Sichuan University Chengdu, China., Long HY; Department of Pulmonary Diseases, State Key Laboratory of Biotherapy of China and Department of Respiratory Medicine, West China Hospital of Sichuan University Chengdu, China., Wang H; Department of Pulmonary Diseases, State Key Laboratory of Biotherapy of China and Department of Respiratory Medicine, West China Hospital of Sichuan University Chengdu, China., Luo ZY; West China School of Medicine, Sichuan University Chengdu, China., Wei ZX; West China School of Medicine, Sichuan University Chengdu, China., Hu SQ; West China School of Medicine, Sichuan University Chengdu, China., Wen FQ; Department of Pulmonary Diseases, State Key Laboratory of Biotherapy of China and Department of Respiratory Medicine, West China Hospital of Sichuan University Chengdu, China.

المصدر: International journal of clinical and experimental medicine [Int J Clin Exp Med] 2014 May 15; Vol. 7 (5), pp. 1289-96. Date of Electronic Publication: 2014 May 15 (Print Publication: 2014).

نوع المنشور: Journal Article

بيانات الدورية: Publisher: e-Century Pub. Corp Country of Publication: United States NLM ID: 101471010 Publication Model: eCollection Cited Medium: Print ISSN: 1940-5901 (Print) Linking ISSN: 19405901 NLM ISO Abbreviation: Int J Clin Exp Med Subsets: PubMed not MEDLINE

مستخلص: It is reported that osteopontin has shown promising diagnostic value for malignant pleural mesothelioma (MPM), this meta-analysis aimed to establish the overall diagnostic accuracy of the osteopontin measurement for diagnosing MPM. Based on a systematic review of English language studies, the sensitivity, specificity and other measures of accuracy of osteopontin in the diagnosis of MPM were pooled using random-effects model. Summary receiver operating characteristic curves were used to summarize overall test performance. Seven publications met our inclusion criteria, the pooled sensitivity was 0.57 (95%CI: 0.52-0.61), specificity was 0.81, 95%CI: 0.79-0.84). The PLR was 3.78 (95%CI: 2.23-6.41), the NLR was 0.51 (95%CI: 0.38-0.67) and the DOR was 9.04 (95%CI: 5.28-15.48), the area under the summary receiver operating characteristic curve was 0.80. Our data suggest that osteopontin is likely to be a useful diagnostic marker for MPM, considering for the limited studies and patients included, larger studies are needed to confirm these findings.