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1دورية أكاديمية
المؤلفون: Yen-Ju Chu, Lee-Chin Wong, Che-Sheng Ho, Jia-Yun Huang, I-Chun Lee, Hsin-Pei Wang, Cheng-Hsien Huang, Chia-Jui Hsu, Wen-Hsin Hsu, Yu-Chia Kao, Bi-Chun Duan, Inn-Chi Lee, Yung-Ting Kuo, Fu-Man Chang, Su-Ching Hu, Chang-Chun Wu, Lung-Chang Lin, Wan-Ling Hsiao, Chuan-Yu Wang, Kun-Long Hung, Hsiao-Ju Chi, Shi-Bing Wong, Wang-Tso Lee
المصدر: Journal of the Formosan Medical Association, Vol 123, Iss 7, Pp 811-817 (2024)
مصطلحات موضوعية: COVID-19, Pediatrics, Encephalomyelitis, Acute disseminated, Seizures, Myoclonus, Medicine (General), R5-920
الوصف: Background: The SARS-CoV-2 virus has been a global public health threat since December 2019. This study aims to investigate the neurological characteristics and risk factors of coronavirus disease 2019 (COVID-19) in Taiwanese children, using data from a collaborative registry. Methods: A retrospective, cross-sectional, multi-center study was done using an online network of pediatric neurological COVID-19 cohort collaborative registry. Results: A total of 11160 COVID-19-associated emergency department (ED) visits and 1079 hospitalizations were analyzed. Seizures were the most common specific neurological symptom, while encephalitis and acute disseminated encephalomyelitis (ADEM) was the most prevalent severe involvement.In ED patients with neurological manifestations, severe neurological diagnosis was associated with visual hallucination, seizure with/without fever, behavior change, decreased GCS, myoclonic jerk, decreased activity/fatigue, and lethargy. In hospitalized patients with neurological manifestations, severe neurological diagnosis was associated with behavior change, visual hallucination, decreased GCS, seizure with/without fever, myoclonic jerk, fatigue, and hypoglycemia at admission. Encephalitis/ADEM was the only risk factor for poor neurological outcomes at discharge in hospitalized patients. Conclusion: Neurological complications are common in pediatric COVID-19. Visual hallucination, seizure, behavior change, myoclonic jerk, decreased GCS, and hypoglycemia at admission are the most important warning signs of severe neurological involvement such as encephalitis/ADEM.
وصف الملف: electronic resource
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2دورية أكاديمية
المؤلفون: Sargun Sokhi, Cody W. Lewis, Amirali B. Bukhari, Joanne Hadfield, Edric J. Xiao, Jeremy Fung, Yea Jin Yoon, Wen-Hsin Hsu, Armin M. Gamper, Gordon K. Chan
المصدر: Frontiers in Cell and Developmental Biology, Vol 11 (2023)
مصطلحات موضوعية: cell cycle, cell cycle checkpoint kinase inhibitors, DNA damage checkpoint kinase inhibitors, Myt1 overexpression, resistance, Biology (General), QH301-705.5
الوصف: Cell cycle checkpoint kinases serve as important therapeutic targets for various cancers. When they are inhibited by small molecules, checkpoint abrogation can induce cell death or further sensitize cancer cells to other genotoxic therapies. Particularly aberrant Cdk1 activation at the G2/M checkpoint by kinase inhibitors causing unscheduled mitotic entry and mitotic arrest was found to lead to DNA damage and cell death selectively in cancer cells. Promising drugs inhibiting kinases like Wee1 (Adavosertib), Wee1+Myt1 (PD166285), ATR (AZD6738) and Chk1 (UCN-01) have been developed, but clinical data has shown variable efficacy for them with poorly understood mechanisms of resistance. Our lab recently identified Myt1 as a predictive biomarker of acquired resistance to the Wee1 kinase inhibitor, Adavosertib. Here, we investigate the role of Myt1 overexpression in promoting resistance to inhibitors (PD166285, UCN-01 and AZD6738) of other kinases regulating cell cycle progression. We demonstrate that Myt1 confers resistance by compensating Cdk1 inhibition in the presence of these different kinase inhibitors. Myt1 overexpression leads to reduced premature mitotic entry and decreased length of mitosis eventually leading to increased survival rates in Adavosertib treated cells. Elevated Myt1 levels also conferred resistance to inhibitors of ATR or Chk1 inhibitor. Our data supports that Myt1 overexpression is a common mechanism by which cancer cells can acquire resistance to a variety of drugs entering the clinic that aim to induce mitotic catastrophe by abrogating the G2/M checkpoint.
وصف الملف: electronic resource
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3دورية أكاديمية
المؤلفون: Cheng-Tang Pan, Wen-Hsin Hsu, Yu-Shun Cheng, Zhi-Hong Wen, Wen-Fan Chen
المصدر: Micromachines, Vol 12, Iss 11, p 1294 (2021)
مصطلحات موضوعية: critical bone defect, composite scaffold, porosity, Ti-6Al-4V, hydroxyapatite, finite element analysis, Mechanical engineering and machinery, TJ1-1570
الوصف: The tibia of New Zealand White rabbits was used as a model of critical bone defects to investigate a new design of composite scaffold for bone defects composed of dual materials. The all-in-one design of a titanium alloy (Ti-6Al-4V) scaffold comprised the structure of a bone plate and gradient porosity cage. Hydroxyapatite (HAp), a biodegradable material, was encapsulated in the center of the scaffold. The gradient pore structure was designed with 70%-65%-60%-55%-50% porosity, since the stresses could be distributed more uniformly when the all-in-one scaffold was placed on the bone contact surface. By covering the center of the scaffold with a low strength of HAp to contact the relatively low strength of bone marrow tissues, the excessive stiffness of the Ti-6Al-4V can be effectively reduced and further diminish the incidence of the stress shielding effect. The simulation results show that the optimized composite scaffold for the 3D model of tibia had a maximum stress value of 27.862 MPa and a maximum strain of 0.065%. The scaffold prepared by selective laser melting was annealed and found that the Young’s coefficient increased from 126.44 GPa to 131.46 GPa, the hardness increased from 3.9 GPa to 4.12 GPa, and the strain decreased from 2.27% to 1.13%. The result demonstrates that the removal of residual stress can lead to a more stable structural strength, which can be used as a reference for the design of future clinical tibial defect repair scaffolds.
وصف الملف: electronic resource
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4دورية أكاديمية
المؤلفون: Yung-Chiang Chen, Wen-Hsin Hsu, Chun-Ming Sun, Chao-Hong Liu
المصدر: Dermatologica Sinica, Vol 36, Iss 3, Pp 146-148 (2018)
مصطلحات موضوعية: Dermatology, RL1-803
الوصف: Aquagenic urticaria, a type of physical urticaria, is quite rare and only about 50 cases have been reported in the medical literature. It was first described by Shelley and Rawnsley in 1964. Wheals occur when a patient’s skin makes contact with any type of water within 30 minutes of exposure, and can last for 30 minutes to 2 hours after cessation of exposure. Aquagenic urticaria most commonly develops on the trunk and upper limbs. It is sometimes associated with pruritus and an uncomfortable prickling or burning sensation. To the best of our knowledge, no case of aquagenic urticaria from Taiwan has been reported. Herein, we present a young Taiwanese male patient diagnosed with aquagenic urticaria.
وصف الملف: electronic resource
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5دورية أكاديمية
المؤلفون: Chia-Mei Liu, Wen-Hsin Hsu, Wan-Yi Lin, Hong-Chen Chen
المصدر: Journal of Biomedical Science, Vol 24, Iss 1, Pp 1-11 (2017)
مصطلحات موضوعية: Adducin, Nuclear localization, Nuclear export, RNA polymerase, ZNF331, Medicine
الوصف: Abstract Background The adducin (ADD) family proteins, namely ADD1, ADD2, and ADD3, are actin-binding proteins that play important roles in the stabilization of membrane cytoskeleton and cell-cell junctions. All the ADD proteins contain a highly conserved bipartite nuclear localization signal (NLS) at the carboxyl termini, but only ADD1 can localize to the nucleus. The reason for this discrepancy is not clear. Methods To avoid the potential effect of cell-cell junctions on the distribution of ADD proteins, HA epitope-tagged ADD proteins and mutants were transiently expressed in NIH3T3 fibroblasts and their distribution in the cytoplasm and nucleus was examined by immunofluorescence staining. Several nuclear proteins were identified to interact with ADD1 by mass spectrometry, which were further verified by co-immunoprecipitation. Results In this study, we found that ADD1 was detectable both in the cytoplasm and nucleus, whereas ADD2 and ADD3 were detected only in the cytoplasm. However, ADD2 and ADD3 were partially (~40%) sequestered in the nucleus by leptomycin B, a CRM1/exportin1 inhibitor. Upon the removal of leptomycin B, ADD2 and ADD3 re-distributed to the cytoplasm. These results indicate that ADD2 and ADD3 possess functional NLS and are quickly transported to the cytoplasm upon entering the nucleus. Indeed, we found that ADD2 and ADD3 possess much higher potential to counteract the activity of the NLS derived from Simian virus 40 large T-antigen than ADD1. All the ADD proteins appear to contain multiple nuclear export signals mainly in their head and neck domains. However, except for the leucine-rich motif (377FEALMRMLDWLGYRT391) in the neck domain of ADD1, no other classic nuclear export signal was identified in the ADD proteins. In addition, the nuclear retention of ADD1 facilitates its interaction with RNA polymerase II and zinc-finger protein 331. Conclusions Our results suggest that ADD2 and ADD3 possess functional NLS and shuttle between the cytoplasm and nucleus. The discrepancy in the subcellular localization of the ADD isoforms arises due to their different nuclear export capabilities. In addition, the interaction of ADD1 with RNA polymerase II and zinc-finger protein 331 implicates a potential role for ADD1 in the regulation of transcription.
وصف الملف: electronic resource
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6دورية أكاديمية
المؤلفون: Wen-Hsin Hsu, Meng-Shun Sun, Hoi-Wan Lo, Ching-Yang Tsai, Yu-Jou Tsai
المصدر: Kaohsiung Journal of Medical Sciences, Vol 28, Iss 5, Pp 265-269 (2012)
مصطلحات موضوعية: Carbon dioxide insufflation, Colonoscopy, Post-colonoscopy discomfort, Medicine (General), R5-920
الوصف: In colonoscopy, the question of when and how to use carbon dioxide (CO2) insufflation remains uncertain. Inspection for the pathological changes during colonoscopy takes place during the withdrawal of the scope. This study aimed to determine whether CO2 insufflation only at the withdrawal of the colonoscope has an effect comparable to that of CO2 usage throughout the course of the procedure. Symptomatic patients were randomized in three groups: (1) patients given air insufflation (A; n = 33); (2) patients given CO2 insufflation only at the time of scope withdrawal (CW; n = 33); and (3) patients given the CO2 insufflation (C; n = 34) for the whole course of the colonoscopy. Patients were requested to answer questionnaires about their pain score during, at the end, and 1 h after the colonoscopy by using a pain numerical scale ranging from 0 to 10. The disparities of the pain score were noted at the end of the procedure and 1 h after the procedure (p = 0.026 and p
وصف الملف: electronic resource
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7دورية أكاديمية
المؤلفون: Wen-Hsin Hsu, Meng-Shun Sun, Hoi-Wan Lo, Ching-Yang Tsai, Yu-Jou Tsai
المصدر: Gastroenterology Research and Practice, Vol 2013 (2013)
مصطلحات موضوعية: Diseases of the digestive system. Gastroenterology, RC799-869
الوصف: Objectives. Endoscopic submucosal dissection (ESD) for early colorectal neoplasms is regarded as a difficult technique and should commence after receiving the experiences of ESD in the stomach. The implementation of colorectal ESD in countries where early gastric cancer is uncommon might therefore be difficult. The aim is to delineate the feasibility and the learning curve of colorectal ESD performed by a colonoscopist with limited experience of gastric ESD. Methods. The first fifty cases of colorectal ESD, which were performed by a single colonoscopist between July 2010 and April 2013, were enrolled. Results. The mean of age was 64 (±9.204) years with mean size of neoplasm at 33 (±12.63) mm. The mean of procedure time was 70.5 (±48.9) min. The rates of en bloc resection, R0 resection, and curative resection were 86%, 86%, and 82%, respectively. Three patients had immediate perforation, but no patient developed delayed perforation or delayed bleeding. Conclusion. Our result disclosed that it is feasible for colorectal ESD to be performed by a colonoscopist with little experience of gastric ESD through satisfactory training and adequate case selection.
وصف الملف: electronic resource
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8دورية أكاديمية
المؤلفون: Audrey Wen-Hsin Hsu, Chih-Hsien Liao
المصدر: Springer, Review of Quantitative Finance and Accounting. 60(2):607-641
الوصف: This study examines whether audit partners’ industry specialization could reduce real activity earnings management (RM). We argue that partners with industry specialization can reduce RM because they can better assess their clients’ business risk, and will more protect their reputation than other auditors. However, the extent to which auditors can constrain RM depends on whether the type of RM can affect accruals and internal controls. Focusing on two types of RM, we find that individual audit partners’ industry expertise is negatively associated with overproduction, but do not find evidence that audit partners’ industry specialization reduces firms’ abnormal reduction of discretionary expenditures. Cross-sectional analysis shows that the effect of audit partners’ industry expertise on overproduction is stronger when the firm has better corporate governance and has at least one director with auditor-related experience on the board. These suggest that strong governance and director expertise act as a complement mechanism that facilitates auditors’ communication with corporate managers. Overall, the findings suggest that auditors with industry expertise care about their reputation in detecting accruals and thus put more efforts in constraining overproduction, but not in cutting discretionary expenses.
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المؤلفون: Chen-Tsen Yeh, Han-Wen Chang, Wen-Hsin Hsu, Shing-Jong Huang, Meng-Hsin Wu, Ling-Hsien Tu, Ming-Che Lee, Jerry Chun Chung Chan
الوصف: The underlying biophysical principle governing the cytotoxicity of the oligomeric aggregates of β-amyloid (Aβ) peptides has long been an enigma. Here we show that the size of Aβ40oligomers can be actively controlled by incubating the peptides in reverse micelles. Our approach allowed for the first time a detailed comparison of the structures and dynamics of two Aβ40oligomers of different size, viz., 10 and 23 nm, by solid-state NMR. From the chemical shift data, we infer that the conformation of the residues from K16 to K28 are different between the 10-nm and 23-nm oligomers. We find that the 10-nm oligomers are more cytotoxic, and the molecular motions of their charged residues are more dynamic. Interestingly, the residue A21 exhibits an unusually high structural rigidity. Our data raise the interesting possibility that the cytotoxicity of Aβ40oligomers could also be correlated to the motional dynamics of the charged residues.
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_________::dd24dea19cf21984444e9b1165e105f3
https://doi.org/10.1101/2023.05.04.539338 -
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المؤلفون: Jason Lequyer, Reuben Philip, Amit Sharma, Wen-Hsin Hsu, Laurence Pelletier
المصدر: Nature Machine Intelligence. 4:953-963
مصطلحات موضوعية: Human-Computer Interaction, Artificial Intelligence, Computer Networks and Communications, Computer Vision and Pattern Recognition, Software
الوصف: Image noise is a common problem in light microscopy. This is particularly true in real-time live-cell imaging applications in which long-term cell viability necessitates low-light conditions. Modern denoisers are typically trained on a representative dataset, sometimes consisting of just unpaired noisy shots. However, when data are acquired in real time to track dynamic cellular processes, it is not always practical nor economical to generate these training sets. Recently, denoisers have emerged that allow us to denoise single images without a training set or knowledge about the underlying noise. But such methods are currently too slow to be integrated into imaging pipelines that require rapid, real-time hardware feedback. Here we present Noise2Fast, which can overcome these limitations. Noise2Fast uses a novel downsampling technique we refer to as ‘chequerboard downsampling’. This allows us to train on a discrete 4-image training set, while convergence can be monitored using the original noisy image. We show that Noise2Fast is faster than all similar methods with only a small drop in accuracy compared to the gold standard. We integrate Noise2Fast into real-time multi-modal imaging applications and demonstrate its broad applicability to diverse imaging and analysis pipelines.
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