المؤلفون: Guangling Shui, Hairong Lin, Xiaomei Ma, Bo Zhu, Peng Han, Nurimanguli Aini, Chunping Guo, Yuanlong Wu, Zhenyuan Pan, Chunyuan You, Guoli Song, Xinhui Nie

المصدر: Journal of Cotton Research, Vol 7, Iss 1, Pp 1-4 (2024)

مصطلحات موضوعية: Plant culture, SB1-1110

وصف الملف: electronic resource

Relation: https://doaj.org/toc/2523-3254

URL الوصول: https://doaj.org/article/a0bf8ca8a72f4bab83a3793da436a74b

المؤلفون: Ryan Sutherland, Cary P. Gross, Xiaomei Ma, Farah Jeong, Tyler M. Seibert, Matthew R. Cooperberg, William J. Catalona, Shellie D. Ellis, Stacy Loeb, Dena Schulman‐Green, Michael S. Leapman

المصدر: BJUI Compass, Vol 5, Iss 6, Pp 593-601 (2024)

مصطلحات موضوعية: active surveillance, patient‐centered research, prostate cancer, prostate MRI, qualitative research, Diseases of the genitourinary system. Urology, RC870-923

الوصف: Abstract Introduction Although prostate magnetic resonance imaging (MRI) is commonly used in the diagnosis, staging and active surveillance of prostate cancer, little is known about patient perspectives on MRI. Methods We performed a qualitative study consisting of in‐depth, semi‐structured interviews of patients with low‐ and intermediate‐risk prostate cancer managed with active surveillance. Interviews focused on experiences with and knowledge of prostate MRI and MRI‐ultrasound fusion biopsy during active surveillance. We purposively sampled patients who received prostate MRI as part of their clinical care, conducted interviews until reaching thematic saturation and performed conventional content analysis to analyse data. Results Twenty patients aged 51–79 years (mean = 68 years) participated in the study. At diagnosis, 17 (85%) had a Gleason grade group 1, and three (15%) had a grade group 2 tumour. Overall, participants viewed prostate MRI as a valuable tool that accurately localizes and monitors prostate cancer over time, and they considered prostate MRI central to active surveillance monitoring. We identified five thematic categories related to MRI use: (1) the experiential aspects of undergoing an MRI scan; (2) the experience of visualizing one's own prostate and prostate cancer; (3) adequacy of provider explanations of MRI results; (4) confidence in prostate MRI in decision‐making; and (5) the role of prostate MRI in longitudinal follow‐up, including an interest in using MRI to modify the timing of, or replace, prostate biopsy. Conclusion Patients value prostate MRI as a tool that enhances their confidence in the initial diagnosis and monitoring of prostate cancer. This work can inform future studies to optimize patient experience, education and counselling during active surveillance for prostate cancer.

وصف الملف: electronic resource

Relation: https://doaj.org/toc/2688-4526

URL الوصول: https://doaj.org/article/a716092646f0417b8cff9026b974b943

المؤلفون: Guangling Shui, Hairong Lin, Xiaomei Ma, Bo Zhu, Peng Han, Nurimanguli Aini, Chunping Guo, Yuanlong Wu, Zhenyuan Pan, Chunyuan You, Guoli Song, Xinhui Nie

المصدر: Journal of Cotton Research, Vol 7, Iss 1, Pp 1-10 (2024)

مصطلحات موضوعية: SSR, Genome wide association studies, Abscission, Favorable alleles, Cotton, Genetic improvement, Plant culture, SB1-1110

الوصف: Abstract Background Cotton is an economically important crop. It is crucial to find an effective method to improve cotton yield, and one approach is to decrease the abscission of cotton bolls and buds. However, the lack of knowledge of the genetic and molecular mechanisms underlying cotton boll abscission traits has hindered genetic improvements. Results Pearson’s correlation analysis revealed a significant positive correlation between boll abscission rates 1 (AR1) and boll abscission rates 2 (AR2). A genome-wide association study was conducted on 145 loci that exhibited high polymorphism and were uniformly distributed across 26 chromosomes (pair). The study revealed 18, 46, and 62 markers that were significantly associated with boll abscission, fiber quality, and yield traits (P

وصف الملف: electronic resource

Relation: https://doaj.org/toc/2523-3254

URL الوصول: https://doaj.org/article/eda788d9ae3049aaaa1d86954826a0d6

المؤلفون: Haoqin Ke, Jonathan D. Bohbot, Yongjuan Chi, Shiwen Duan, Xiaomei Ma, Bingzhong Ren, Yinliang Wang

المصدر: Communications Biology, Vol 7, Iss 1, Pp 1-9 (2024)

مصطلحات موضوعية: Biology (General), QH301-705.5

الوصف: Abstract In Asian honeybees, virgin queens typically only mate during a single nuptial flight before founding a colony. This behavior is controlled by the queen-released mandibular pheromone (QMP). 9-oxo-(E)-2-decenoic acid (9-ODA), a key QMP component, acts as sex pheromone and attracts drones. However, how the queens prevent additional mating remains elusive. Here, we show that the secondary QMP component methyl p-hydroxybenzoate (HOB) released by mated queens inhibits male attraction to 9-ODA. Results from electrophysiology and in situ hybridization assay indicated that HOB alone significantly reduces the spontaneous spike activity of 9-ODA-sensitive neurons, and AcerOr11 is specifically expressed in sensilla placodea from the drone’s antennae, which are the sensilla that narrowly respond to both 9-ODA and HOB. Deorphanization of AcerOr11 in Xenopus oocyte system showed 9-ODA induces robust inward (regular) currents, while HOB induces inverse currents in a dose-dependent manner. This suggests that HOB potentially acts as an inverse agonist against AcerOr11.

وصف الملف: electronic resource

Relation: https://doaj.org/toc/2399-3642

URL الوصول: https://doaj.org/article/f7f2b52676a34130a045922f226e0b8b

المؤلفون: Xinping Dong, Xiaomei Ma, Zhilong Zhao, Miao Ma

المصدر: BMC Plant Biology, Vol 24, Iss 1, Pp 1-17 (2024)

مصطلحات موضوعية: Liquorice, Salinity, Salt secretion, Physiological mechanism, Glycine betaine, Botany, QK1-989

الوصف: Abstract Background Glycyrrhiza uralensis Fisch., a valuable medicinal plant, shows contrasting salt tolerance between seedlings and perennial individuals, and salt tolerance at seedling stage is very weak. Understanding this difference is crucial for optimizing cultivation practices and maximizing the plant’s economic potential. Salt stress resistance at the seedling stage is the key to the cultivation of the plant using salinized land. This study investigated the physiological mechanism of the application of glycine betaine (0, 10, 20, 40, 80 mM) to seedling stages of G. uralensis under salt stress (160 mM NaCl). Results G. uralensis seedlings’ growth was severely inhibited under NaCl stress conditions, but the addition of GB effectively mitigated its effects, with 20 mM GB had showing most significant alleviating effect. The application of 20 mM GB under NaCl stress conditions significantly increased total root length (80.38%), total root surface area (93.28%), and total root volume (175.61%), and significantly increased the GB content in its roots, stems, and leaves by 36.88%, 107.05%, and 21.63%, respectively. The activity of betaine aldehyde dehydrogenase 2 (BADH2) was increased by 74.10%, 249.38%, and 150.60%, respectively. The 20 mM GB-addition treatment significantly increased content of osmoregulatory substances (the contents of soluble protein, soluble sugar and proline increased by 7.05%, 70.52% and 661.06% in roots, and also increased by 30.74%, 47.11% and 26.88% in leaves, respectively.). Furthermore, it markedly enhanced the activity of antioxidant enzymes and the content of antioxidants (SOD, CAT, POD, APX and activities and ASA contents were elevated by 59.55%, 413.07%, 225.91%, 300.00% and 73.33% in the root, and increased by 877.51%, 359.89%, 199.15%, 144.35%, and 108.11% in leaves, respectively.), and obviously promoted salt secretion capacity of the leaves, which especially promoted the secretion of Na+ (1.37 times). Conclusions In summary, the exogenous addition of GB significantly enhances the salt tolerance of G. uralensis seedlings, promoting osmoregulatory substances, antioxidant enzyme activities, excess salt discharge especially the significant promotion of the secretion of Na+Future studies should aim to elucidate the molecular mechanisms that operate when GB regulates saline stress tolerance.

وصف الملف: electronic resource

Relation: https://doaj.org/toc/1471-2229

URL الوصول: https://doaj.org/article/32f88b0124bf4e0fb57c1fde4e92e971

المؤلفون: Song Shen, Tianyong Sun, Xiangjiu Ding, Xiufeng Gu, Yushang Wang, Xiaomei Ma, Zixuan Li, Haiting Gao, Shaohua Ge, Qiang Feng

المصدر: Journal of Advanced Research, Vol 57, Iss , Pp 93-105 (2024)

مصطلحات موضوعية: Fusobacterium nucleatum, Lipid deposition, Gbp, CypA, RNA-seq, Drug repositioning, Medicine (General), R5-920, Science (General), Q1-390

الوصف: Introduction: Growing evidence has shown the correlation between periodontitis and atherosclerosis, while our knowledge on the pathogenesis of periodontitis-promoting atherosclerosis is far from sufficient. Objectives: Illuminate the pathogenic effects of Fusobacterium nucleatum (F. nucleatum) on intracellular lipid deposition in THP-1-derived macrophages and elucidate the underlying pathogenic mechanism of how F. nucleatum promoting atherosclerosis. Methods and results: F. nucleatum was frequently detected in different kinds of atherosclerotic plaques and its abundance was positively correlated with the proportion of macrophages. In vitro assays showed F. nucleatum could adhere to and invade THP-1 cells, and survive continuously in macrophages for 24 h. F. nucleatum stimulation alone could significantly promote cellular inflammation, lipid uptake and inhibit lipid outflow. The dynamic gene expression of THP-1 cells demonstrated that F. nucleatum could time-serially induce the over-expression of multiple inflammatory related genes and activate NF-κB, MAPK and PI3K-AKT signaling pathways. The exoprotein of F. nucleatum, D-galactose-binding protein (Gbp), acted as one of the main pathogenic proteins to interact with the Cyclophilin A (CypA) of THP-1 cells and induced the activation of the NF- κB, MAPK and PI3K-AKT signaling pathways. Furthermore, use of six candidate drugs targeting to the key proteins in NF- κB, MAPK and PI3K-AKT pathways could dramatically decrease F. nucleatum induced inflammation and lipid deposition in THP-1 cells. Conclusions: This study suggests that the periodontal pathogen F. nucleatum can activate macrophage PI3K-AKT/MAPK/NF-κB signal pathways, promotes inflammation, enhances cholesterol uptake, reduces lipid excretion, and promotes lipid deposition, which may be one of its main strategies promoting the development of atherosclerosis.

وصف الملف: electronic resource

Relation: http://www.sciencedirect.com/science/article/pii/S2090123223001133; https://doaj.org/toc/2090-1232

URL الوصول: https://doaj.org/article/a0f262ccb52b4151abdcefc067981a06

المؤلفون: Jianqiao Jiang, Ruonan Yin, Yun Xie, Xiaomei Ma, Miao Cui, Yiwen Chen, Yongkang Li, Yue Hu, Jianming Niu, Weidong Cheng, Feifei Gao

المصدر: Food Chemistry: X, Vol 22, Iss , Pp 101502- (2024)

مصطلحات موضوعية: Mixed fermentation, Lactic acid bacteria, Prune wine, Phenolic compounds, Flavor compounds, Nutrition. Foods and food supply, TX341-641, Food processing and manufacture, TP368-456

الوصف: To determine the effect of cofermentation of Saccharomyces cerevisiae and different LABs on prune wine quality, this study compared phenolic compounds, organic acids, soluble sugars, biogenic amines and volatile flavor compounds among different treatments. The results showed that inoculation of LAB increased DPPH and total flavonoid content. Malic acid content was reduced in HS, HB and HF. Histamine content in S, F and B was lower than the limits in French and Australian wines. 15 phenolic compounds were identified. Yangmeilin and chlorogenic acid were detected only in HS, HF and HB. 51 volatile flavor compounds were identified, esters being the most diverse and abundant. 14 volatile flavor compounds with OAV > 1 contributed highly to the aroma of prune wine. 9 chemical markers including resveratrol, rutin, and catechin were screened to explain intergroup differences by OPLS-DA. This study provides new insights into the processing and quality analysis of prunes.

وصف الملف: electronic resource

Relation: http://www.sciencedirect.com/science/article/pii/S2590157524003894; https://doaj.org/toc/2590-1575

URL الوصول: https://doaj.org/article/2f789c8aa1934bf5a043125d65246b88

المؤلفون: Xiaoyu Ma, Ying Wang, Qiong Wu, Xiaomei Ma, Qiang Wang, Qinghong Guo

المصدر: Frontiers in Cellular and Infection Microbiology, Vol 14 (2024)

مصطلحات موضوعية: neurobrucellosis, myelitis, MetaCAP™, ganglioside, hormone therapy, Microbiology, QR1-502

الوصف: Brucellosis is a zoonotic disease caused by a Gram-negative coccus a facultative intracellular pathogen. Neurobrucellosis has an incidence rate of 3-7% among all patients with brucellosis, while spinal cord involvement is rare and carries a significant mortality risk. This report describes a case of brucellosis myelitis in a 55-year-old male patient who presented with recurrent paralysis, incontinence, and damage to the visual and auditory nerves. The diagnosis of neurobrucellosis involves a serum tube agglutination test, cerebrospinal fluid analysis, a physical examination of the nervous system, and a comprehensive review of the patient’s medical history. The presence of brucellosis was confirmed in cerebrospinal fluid using MetaCAP™ sequencing. Treatment with a combination of rifampicin, doxycycline, ceftriaxone sodium, amikacin, compound brain peptide ganglioside, and dexamethasone resulted in significant improvement of the patient’s clinical symptoms and a decrease in the brucellosis sequence count in cerebrospinal fluid. For the first time, MetaCAP™ sequencing has been used to treat pathogenic microbial nucleic acids, which could be a valuable tool for early diagnosis and treatment of neurobrucellosis.

وصف الملف: electronic resource

Relation: https://www.frontiersin.org/articles/10.3389/fcimb.2024.1378331/full; https://doaj.org/toc/2235-2988

URL الوصول: https://doaj.org/article/dbc916d74b934f8f8377ac597149f01e

المؤلفون: Xiaomei Ma, Yongqian Liu, Jie Yan, Shuang Han, Li Li, Hang Meng, Muhammet Deveci, Konstanze Kolle, Umit Cali

المصدر: CSEE Journal of Power and Energy Systems, Vol 10, Iss 1, Pp 76-87 (2024)

مصطلحات موضوعية: Annual energy production, atmospheric stability, data sources, offshore wind resource, wind power density, Technology, Physics, QC1-999

الوصف: Traditional assessment indexes could not fully describe offshore wind resources, for the meteorological properties of offshore are more complex than onshore. As a result, the uncertainty of offshore wind power projects would be increased and final economic benefits would be affected. Therefore, a study on offshore wind resource assessment is carried out, including three processes of “studying data sources, conducting multi-dimensional indexes system and proposing an offshore wind resource assessment method based on analytic hierarchy process (AHP)”. First, measured wind data and two kinds of reanalysis data are used to analyze the characteristics and reliability of data sources. Second, indexes such as effective wind speed occurrence, affluent level occurrence, coefficient of variation, neutral state occurrence have been proposed to depict availability, richness, and stability of offshore wind resources, respectively. Combined with existing parameters (wind power density, dominant wind direction occurrence, water depth, distance to coast), a multi-dimensional indexes system has been built and on this basis, an offshore wind energy potential assessment method has been proposed. Furthermore, the proposed method is verified by the annual energy production of five offshore wind turbines and practical operating data of four offshore wind farms in China. This study also compares the ranking results of the AHP model to two multi-criteria decision making (MCDM) models including weighted aggregated sum product assessment (WASPAS) and multi-attribute ideal real comparative analysis (MAIRCA). Results show the proposed method gains well in practical engineering applications, where the economic score values have been considered based on the offshore reasonable utilization hours of the whole life cycle in China.

وصف الملف: electronic resource

Relation: https://ieeexplore.ieee.org/document/9917401/; https://doaj.org/toc/2096-0042

URL الوصول: https://doaj.org/article/0ee8b2fa2a894f22991d1b28c2b0d9fb

المؤلفون: Michael S. Leapman, Ryan Sutherland, Cary P. Gross, Xiaomei Ma, Tyler M. Seibert, Matthew R. Cooperberg, William J. Catalona, Stacy Loeb, Dena Schulman‐Green

المصدر: BJUI Compass, Vol 5, Iss 1, Pp 142-149 (2024)

مصطلحات موضوعية: active surveillance, biomarkers, decision‐making, genomic testing, prostate cancer, qualitative, Diseases of the genitourinary system. Urology, RC870-923

الوصف: Abstract Background Tissue‐based gene expression (genomic) tests provide estimates of prostate cancer aggressiveness and are increasingly used for patients considering or engaged in active surveillance. However, little is known about patient experiences with genomic testing and its role in their decision‐making. Methods We performed a qualitative study consisting of in‐depth, semi‐structured interviews of patients with low‐ or favourable‐intermediate‐risk prostate cancer managed with active surveillance. We purposively sampled to include patients who received biopsy‐based genomic testing as part of clinical care. The interview guide focused on experiences with genomic testing during patients' decision‐making for prostate cancer management and understanding of genomic test results. We continued interviews until thematic saturation was reached, iteratively created a code key and used conventional content analysis to analyse data. Results Participants' (n = 20) mean age was 68 years (range 51–79). At initial biopsy, 17 (85%) had a Gleason grade group 1, and 3 (15%) had a grade group 2 prostate cancer. The decision to undergo genomic testing was driven by both participants and physicians' recommendations; however, some participants were unaware that testing had occurred. Overall, participants understood the role of genomic testing in estimating their prostate cancer risk, and the test results increased their confidence in the decision for active surveillance. Participants had some misconceptions about the difference between tissue‐based gene expression tests and germline genetic tests and commonly believed that tissue‐based tests measured hereditary cancer risk. While some participants expressed satisfaction with their physicians' explanations, others felt that communication was limited and lacked sufficient detail. Conclusion Patients interact with and are influenced by the results of biopsy‐based genomic testing during active surveillance for prostate cancer, despite gaps in understanding about test results. Our findings indicate areas for improvement in patient counselling in order to increase patient knowledge and comfort with genomic testing.

وصف الملف: electronic resource

Relation: https://doaj.org/toc/2688-4526

URL الوصول: https://doaj.org/article/a14dc37891e3489084198101c440dd61