المؤلفون: Yingping Wu, Tiejun Zhao, Riqiang Deng, Xiaoping Xia, Bin Li, Xunzhang Wang

المصدر: Scientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)

مصطلحات موضوعية: Medicine, Science

الوصف: Abstract To conquer the worldwide outbreak of COVID-19 virus, a large number of studies have been carried out on COVID-19 infection, transmission and treatment. However, few studies have been conducted from the perspectives of circRNA and lncRNA, which are known to be involved in regulating many life activities, such as immune tolerance and immune escapes, and hence may provide invaluable information in the emerging COVID-19 infection and recurrence. Moreover, exosomes has been reported to play an important role in COVID-19 recurrence, and thus may interact with the expression of circRNA and lncRNA. In this work, we sequenced circRNA, lncRNA and mRNA from recurrent COVID-19 patients and healthy people, and compared the differences. GO and KEGG enrichment analysis show that differentially expressed circRNA and lncRNA are mainly involved in the regulation of host cell cycle, apoptosis, immune inflammation, signaling pathway and other processes. The comparison to exosomes related databases shows that there are 114 differentially expressed circRNA, and 10 differentially expressed lncRNA related to exosomes. These studies provide reference for exploring circRNA and lncRNA to study the infection mechanism of COVID-19, their diagnostic and therapeutic values, as well as the possibility to employ them as biomarkers.

وصف الملف: electronic resource

Relation: https://doaj.org/toc/2045-2322

URL الوصول: https://doaj.org/article/557d38faa92f4511b4ba55c4e66a81ab

المؤلفون: Wei Wei, Xianhong Fang, Michael Shehata, Xunzhang Wang, Xianzhang Zhan, Hai Deng, Hongtao Liao, Zili Liao, Yang Liu, Yumei Xue, Shulin Wu

المصدر: Frontiers in Cardiovascular Medicine, Vol 8 (2021)

مصطلحات موضوعية: adenosine triphosphate, programmed electrophysiologic study, catheter ablation, accessory pathways, long-term outcomes, Diseases of the circulatory (Cardiovascular) system, RC666-701

الوصف: Objectives: To study the benefit of adenosine triphosphate (ATP) in evaluating ablation endpoints of accessory pathways (AP) and subsequent long-term prognosis.Methods: We reviewed consecutive patients with supraventricular tachycardias due to APs that underwent radiofrequency catheter ablation (RFCA) from January 2016 to September 2018 in our center. The patients were divided into two groups: the ATP group (who had passed both the ATP test and PES after ablation as the endpoint) and the non-ATP group (who had passed PES only after ablation as the endpoint). We reviewed the patients' intra-cardiac electrograms and analyzed their long-term outcomes.Results: In total, 1,343 patients underwent successful RFCA. There were 215 patients in the ATP group with one lost to follow-up. There were 1,128 patients in the non-ATP group with 39 lost to follow-up. Twenty-three patients in the ATP group demonstrated additional electrophysiological entities due to ATP administration, including reappearance of the ablated APs in 16 patients, discovery of PES-undetected APs in 5, induction of atrial fibrillation in 5, premature atrial contractions in 1, and premature ventricular contractions in another. During the 7 to 39 months (average 24.4 ± 9.5 months) follow-up, the recurrence rate was 8.41% (18/214) in the ATP group and 6.80% (74/1,084) in the non-ATP group. In subjects with recurrence, 14 patients (14/18 = 77.8%) in the ATP group and 50 patients (50/74 = 67.6%) in the non-ATP group accepted redo ablations. Among the ATP-group, all the 14 redo APs were the old ones as before. Among the non-ATP-group, redo ablations confirmed that 39 APs were the old ones, while 20 APs were newly detected ones which had been missed previously. The difference in recurrent AP locations confirmed by redo procedures between the two groups was significant (p = 0.008). In the non-ATP group, 20 (40%) of redo cases were proven to have multiple APs, while 33 (3.3%) cases who did not suffer from recurrence had multiple APs. Existences of multiple APs in recurred cases were significantly higher than that in non-recurred ones in the non-ATP group (p < 0.001), while there was no such difference in the ATP group (p = 0.114).Conclusions: The existence of multiple APs was more common in recurrent cases if ATP was not used for confirmation of ablation endpoints. ATP probably has additional value over PES alone by detecting weak AP conductions. ATP can evoke atrial and ventricular arrhythmias.

وصف الملف: electronic resource

Relation: https://www.frontiersin.org/articles/10.3389/fcvm.2021.716400/full; https://doaj.org/toc/2297-055X

URL الوصول: https://doaj.org/article/b65885668c874096845882cf33076562

المؤلفون: Guangzhi Chen, Yan Wang, Riccardo Proietti, Xunzhang Wang, Feifan Ouyang, Chang Sheng Ma, Rong Hui Yu, Chunxia Zhao, Kezhong Ma, Jie Qiu, Qigong Liu, Dao Wen Wang

المصدر: BMC Cardiovascular Disorders, Vol 20, Iss 1, Pp 1-10 (2020)

مصطلحات موضوعية: Supraventricular tachycardia, Radiofrequency ablation, Zero-fluoroscopy, Radiation exposure, Diseases of the circulatory (Cardiovascular) system, RC666-701

الوصف: Abstract Background Three-dimensional electroanatomic mapping systems have demonstrated a significant reduction in radiation exposure during radiofrequency catheter ablation procedures. We aimed to investigate the safety, feasibility and efficacy of a completely zero-fluoroscopy approach for catheter ablation of supraventricular tachycardia using the Ensite NavX navigation system compared with a conventional fluoroscopy approach. Methods A multicenter prospective non-randomized registry study was performed in seven centers from January 2013 to February 2018. Consecutive patients referred for catheter ablation of supraventricular tachycardia were assigned either to a completely zero-fluoroscopic approach (ZF) or conventional fluoroscopy approach (CF) according to the operator’s preference. Patients with atrial tachycardia were excluded. Results Totally, 1020 patients were enrolled in ZF group; 2040 patients ablated by CF approach were selected for controls. There was no significant difference between the zero-fluoroscopy group and conventional fluoroscopy group as to procedure time (60.3 ± 20.3 vs. 59.7 ± 22.6 min, P = 0.90), immediate success rate of procedure (98.8% vs. 99.2%, P = 0.22), arrhythmia recurrence (0.4% vs. 0.5%, P = 0.85), total success rate of procedure (98.4% vs. 98.8%, P = 0.39) or complications (1.1% vs. 1.5%, P = 0.41). Compared with the conventional fluoroscopy approach, the zero-fluoroscopy approach provided similar outcomes without compromising the safety or efficacy of the procedure. Conclusion The completely zero-fluoroscopy approach demonstrated safety and efficacy comparable to a conventional fluoroscopy approach for catheter ablation of supraventricular tachycardia, and mitigated radiation exposure to both patients and operators. Trial registration clinicaltrials.gov Identifier: NCT03042078 ; first registered February 3, 2017; retrospectively registered.

وصف الملف: electronic resource

Relation: https://doaj.org/toc/1471-2261

URL الوصول: https://doaj.org/article/c11077ea21df4829bdc0f452b473b604

المؤلفون: Jae Hyung Cho, Derek Leong, Natasha Cuk, Joseph E Ebinger, Catherine Bresee, Sung-Han Yoon, Ashkan Ehdaie, Michael Shehata, Xunzhang Wang, Sumeet S Chugh, Eduardo Marbán, Eugenio Cingolani

المصدر: PLoS ONE, Vol 16, Iss 7, p e0254641 (2021)

مصطلحات موضوعية: Medicine, Science

الوصف: Sudden death is the most common mode of mortality in patients with heart failure and preserved ejection fraction (HFpEF). Ventricular arrhythmias (VA) have been suspected as the etiology but the supporting evidence in patients with HFpEF is scarce. We sought to investigate VA prevalence, and to determine if VA are associated with prolonged repolarization, in patients with HFpEF. In a retrospective case-control study design, Cedars-Sinai patients who underwent prolonged ambulatory electrocardiographic monitoring (Zio Patch) between 2016 and 2018 were screened for a clinical diagnosis of HFpEF. Patients with normal diastolic and systolic function who underwent Zio Patch monitoring were also reviewed as controls. Multivariable logistic regression was used to compare the prevalence of rhythm disturbances in patients with and without HFpEF. Ventricular tachycardia (VT) was more prevalent in patients with HFpEF (37% vs. 16% in controls, p = 0.001). Most episodes were non-sustained except for one case of sustained VT in a patient with HFpEF. Covariate-adjusted logistic regression including HFpEF diagnosis, age, sex, body mass index, and the presence of comorbidities revealed that only HFpEF was associated with increased risk of VT (relative risk 2.86, p = 0.023). Subgroup-analyses revealed an association between increased QTc interval and risk of VT (460 ± 38 ms in HFpEF patients with VT vs. 445 ± 28 ms in HFpEF patients without VT, p = 0.03). Non-sustained VT was more prevalent in patients with HFpEF compared to patients without HFpEF, and QTc interval prolongation was associated with VT in HFpEF.

وصف الملف: electronic resource

Relation: https://doaj.org/toc/1932-6203

URL الوصول: https://doaj.org/article/7b372c0a614d48e9bc0e2592d8000c4d

المؤلفون: Jae Hyung Cho, Ali Namazi, Richard Shelton, Archana Ramireddy, Ashkan Ehdaie, Michael Shehata, Xunzhang Wang, Eduardo Marbán, Sumeet S Chugh, Eugenio Cingolani

المصدر: PLoS ONE, Vol 15, Iss 12, p e0244533 (2020)

مصطلحات موضوعية: Medicine, Science

الوصف: Arrhythmias have been reported frequently in COVID-19 patients, but the incidence and nature have not been well characterized. Patients admitted with COVID-19 and monitored by telemetry were prospectively enrolled in the study. Baseline characteristics, hospital course, treatment and complications were collected from the patients' medical records. Telemetry was monitored to detect the incidence of cardiac arrhythmias. The incidence and types of cardiac arrhythmias were analyzed and compared between survivors and non-survivors. Among 143 patients admitted with telemetry monitoring, overall in-hospital mortality was 25.2% (36/143 patients) during the period of observation (mean follow-up 23.7 days). Survivors were less tachycardic on initial presentation (heart rate 90.6 ± 19.6 vs. 99.3 ± 23.1 bpm, p = 0.030) and had lower troponin (peak troponin 0.03 vs. 0.18 ng/ml. p = 0.004), C-reactive protein (peak C-reactive protein 97 vs. 181 mg/dl, p = 0.029), and interleukin-6 levels (peak interleukin-6 30 vs. 246 pg/ml, p = 0.003). Sinus tachycardia, the most common arrhythmia (detected in 39.9% [57/143] of patients), occurred more frequently in non-survivors (58.3% vs. 33.6% in survivors, p = 0.009). Premature ventricular complexes occurred in 28.7% (41/143), and non-sustained ventricular tachycardia in 15.4% (22/143) of patients, with no difference between survivors and non-survivors. Sustained ventricular tachycardia and ventricular fibrillation were not frequent (seen only in 1.4% and 0.7% of patients, respectively). Contrary to reports from other regions, overall mortality was higher and ventricular arrhythmias were infrequent in this hospitalized and monitored COVID-19 population. Either disease or management-related factors could explain this divergence of clinical outcomes, and should be urgently investigated.

وصف الملف: electronic resource

Relation: https://doaj.org/toc/1932-6203

URL الوصول: https://doaj.org/article/207f0cae08f0470e8c0b1abe47ef02a3

المؤلفون: Ming Liang, Zulu Wang, Yi Li, Yanchun Liang, Yuji Zhang, Jingjing Rong, Yang Lv, Qi Zhang, Guitang Yang, Mingyu Sun, Junqi Wang, Sainan Li, Xunzhang Wang, Yaling Han

المصدر: Cardiology Research and Practice, Vol 2020 (2020)

مصطلحات موضوعية: Diseases of the circulatory (Cardiovascular) system, RC666-701

الوصف: Introduction. Purkinje system and false tendons (FTs) are related to ventricular arrhythmia, but the association between Purkinje fibers and FTs is not clear. This study investigated the associations of anatomical and electrophysiological characteristics between Purkinje fibers and FTs. Methods and Results. We optimized the protocol of Lugol’s iodine solution staining of Purkinje fibers to study the anatomical structure and originated a novel electrophysiological mapping method, named the direct visual mapping (DVM) method, to study the electrophysiological characteristics. By using the above-mentioned innovations in 12 dogs, we found the following. (1) There was no Purkinje fiber found 0.5 cm–1.0 cm below the valve annulus or on the leaflets or chordae tendineae of the mitral valve or adjacent to the top 1/3 of the papillary muscle. (2) Purkinje fibers existed in all FTs, including smaller and tiny FTs. (3) The Purkinje fibers contained in the FTs extended from the proximal to the distal end, and their electrophysiological characteristics were similar to the fibers on the endocardium, including anterograde, retrograde, and decremental conduction and automaticity. Conclusions. Purkinje fibers are commonly found in FTs. The electrophysiological characteristics of the Purkinje fibers contained in FTs are similar to the fibers on the endocardium. FTs might have an anatomical and electrophysiological basis for ventricular arrhythmia.

وصف الملف: electronic resource

Relation: https://doaj.org/toc/2090-8016; https://doaj.org/toc/2090-0597

URL الوصول: https://doaj.org/article/cef3d1122a7e4157b2a9185135283d2a

المؤلفون: Ali A. Sovari, MD, Ashkan Ehdaie, MD, Qiang Liu, MS, Xunzhang Wang, MD, Charles Swerdlow, MD, FHRS, Michael Shehata, MD, FHRS

المصدر: HeartRhythm Case Reports, Vol 3, Iss 2, Pp 133-136 (2017)

مصطلحات موضوعية: Subcutaneous defibrillator, Defibrillation, Oversensing, Inappropriate shock, S-ICD, Ventricular tachycardia, Diseases of the circulatory (Cardiovascular) system, RC666-701

وصف الملف: electronic resource

Relation: http://www.sciencedirect.com/science/article/pii/S2214027116301269; https://doaj.org/toc/2214-0271

URL الوصول: https://doaj.org/article/54b6cfcf776d40a5afba854c243f8c6a

المؤلفون: Ivan Cokic, Avinash Kali, Xunzhang Wang, Hsin-Jung Yang, Richard L Q Tang, Anees Thajudeen, Michael Shehata, Allen M Amorn, Enzhao Liu, Brian Stewart, Nathan Bennett, Doron Harlev, Sotirios A Tsaftaris, Warren M Jackman, Sumeet S Chugh, Rohan Dharmakumar

المصدر: PLoS ONE, Vol 8, Iss 9, p e73193 (2013)

مصطلحات موضوعية: Medicine, Science

الوصف: Iron deposition has been shown to occur following myocardial infarction (MI). We investigated whether such focal iron deposition within chronic MI lead to electrical anomalies.Two groups of dogs (ex-vivo (n = 12) and in-vivo (n = 10)) were studied at 16 weeks post MI. Hearts of animals from ex-vivo group were explanted and sectioned into infarcted and non-infarcted segments. Impedance spectroscopy was used to derive electrical permittivity ([Formula: see text]) and conductivity ([Formula: see text]). Mass spectrometry was used to classify and characterize tissue sections with (IRON+) and without (IRON-) iron. Animals from in-vivo group underwent cardiac magnetic resonance imaging (CMR) for estimation of scar volume (late-gadolinium enhancement, LGE) and iron deposition (T2*) relative to left-ventricular volume. 24-hour electrocardiogram recordings were obtained and used to examine Heart Rate (HR), QT interval (QT), QT corrected for HR (QTc) and QTc dispersion (QTcd). In a fraction of these animals (n = 5), ultra-high resolution electroanatomical mapping (EAM) was performed, co-registered with LGE and T2* CMR and were used to characterize the spatial locations of isolated late potentials (ILPs).Compared to IRON- sections, IRON+ sections had higher[Formula: see text], but no difference in[Formula: see text]. A linear relationship was found between iron content and [Formula: see text] (p1.5%)) with similar scar volumes (7.28% ± 1.02% (Iron (1.5%)), p = 0.51) but markedly different iron volumes (1.12% ± 0.64% (Iron (1.5%)), p = 0.02), QT and QTc were elevated and QTcd was decreased in the group with the higher iron volume during the day, night and 24-hour period (p

وصف الملف: electronic resource

Relation: http://europepmc.org/articles/PMC3774668?pdf=render; https://doaj.org/toc/1932-6203

URL الوصول: https://doaj.org/article/3196c0363e9b403b88be36722815c6fc

المؤلفون: Jianhao Ke, Jinwen Wang, Riqiang Deng, Lin Lin, Bei Jinlong, Liao Yaoping, Xunzhang Wang

المصدر: Microbiology Research, Vol 2, Iss 1, Pp e4-e4 (2011)

مصطلحات موضوعية: Microbiology, QR1-502

الوصف: ORF69 (Ac69) of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is conserved in some baculovirus genomes. Although it has been shown that Ac69 has cap 0-dependent methyltransferase activity and is not required for budded virus production in Spodoptera frugiperda Sf-9 cells, its role in occlusion-derived virus synthesis and virus oral infectivity is not known. This paper describes generation of an ac69 knockout AcMNPV bacmid mutant and analyses of the influence of ac69 deletion on the viral infectivity in Sf-9 cells and Trichoplusia ni larvae so as to investigate the role of ac69 in the viral life cycle. Results indicated that ac69 deletion has little effect on the production rates and morphogenesis of budded virus and occlusion-derived virus in Sf-9 cells. In addition, animal experiment revealed that the deletion mutant did not affect AcMNPV infectivity for Trichoplusia ni larvae in LD50 and LT50 bioassay when administered orally. These results suggest that ac69 may be dispensable for viral infectivity both in vitro and in vivo.

وصف الملف: electronic resource

Relation: http://www.pagepress.org/journals/index.php/mr/article/view/2853; https://doaj.org/toc/2036-7473; https://doaj.org/toc/2036-7481

URL الوصول: https://doaj.org/article/17894a0f56364c30b51517424184f5d5

المؤلفون: Xunzhang Wang, Seet, G.G.L., Lau, M.W.S., Low, E., Tan, K.C.

المصدر: OCEANS 2000 MTS/IEEE Conference and Exhibition. Conference Proceedings (Cat. No.00CH37158) OCEANS 2000 OCEANS 2000 MTS/IEEE Conference and Exhibition. 3:2037-2042 vol.3 2000

Relation: OCEANS 2000 MTS/IEEE Conference and Exhibition. Conference Proceedings