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1دورية أكاديمية
المؤلفون: Guoliang Zhang, Yuanchao Sun, Minkai Guan, Mengmeng Liu, Shiduo Sun
المصدر: Cell Communication and Signaling, Vol 22, Iss 1, Pp 1-17 (2024)
مصطلحات موضوعية: Spermatogenesis, Spatiotemporal specificity, Epididymis, Beta-defensin genes, Medicine, Cytology, QH573-671
الوصف: Abstract Low sperm motility is a significant contributor to male infertility. beta-defensins have been implicated in host defence and the acquisition of sperm motility; however, the regulatory mechanisms governing their gene expression patterns and functions remain poorly understood. In this study, we performed single-cell RNA and spatial transcriptome sequencing to investigate the cellular composition of testicular and epididymal tissues and examined their gene expression characteristics. In the epididymis, we found that epididymal epithelial cells display a region specificity of gene expression in different epididymal segments, including the beta-defensin family genes. In particular, Defb15, Defb18, Defb20, Defb25 and Defb48 are specific to the caput; Defb22, Defb23 and Defb26 to the corpus; Defb2 and Defb9 to the cauda of the epididymis. To confirm this, we performed mRNA fluorescence in situ hybridisation (FISH) targeting certain exon region of beta-defensin genes, and found some of their expression matched the sequencing results and displayed a close connection with epididimosome marker gene Cd63. In addition, we paid attention to the Sertoli cells and Leydig cells in the testis, along with fibroblasts and smooth muscle cells in the epididymis, by demonstrating their gene expression profile and spatial information. Our study provides a single-cell and spatial landscape for analysing the gene expression characteristics of testicular and epididymal environments and has important implications for the study of spermatogenesis and sperm maturation.
وصف الملف: electronic resource
Relation: https://doaj.org/toc/1478-811X
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2دورية أكاديمية
المؤلفون: Ke Du, Yuting Liu, Lu Zhang, Lixia Peng, Wenjing Dong, Yajie Jiang, Mingming Niu, Yuanchao Sun, Chuanhong Wu, Yujuan Niu, Yonghe Ding
المصدر: Biomedicine & Pharmacotherapy, Vol 175, Iss , Pp 116637- (2024)
مصطلحات موضوعية: Lapatinib, Doxorubicin, Cardiotoxicity, P38MAPK signaling, Zebrafish embryos, Therapeutics. Pharmacology, RM1-950
الوصف: Because of its enhanced antitumor efficacy, lapatinib (LAP) is commonly used clinically in combination with the anthracycline drug doxorubicin (DOX) to treat metastatic breast cancer. While it is well recognized that this combination chemotherapy can lead to an increased risk of cardiotoxicity in adult women, its potential cardiotoxicity in the fetus during pregnancy remains understudied. Here, we aimed to examine the combination of LAP chemotherapy and DOX-induced cardiotoxicity in the fetus using a zebrafish embryonic system and investigate the underlying pathologic mechanisms. First, we examined the dose-dependent cardiotoxicity of combined LAP and DOX exposure in zebrafish embryos, which mostly manifested as pericardial edema, bradycardia, cardiac function decline and reduced survival. Second, we revealed that a significant increase in oxidative stress concurrent with activated MAPK signaling, as indicated by increased protein expression of phosphorylated p38 and Jnk, was a notable pathophysiological event after combined LAP and DOX exposure. Third, we showed that inhibiting MAPK signaling by pharmacological treatment with the p38MAPK inhibitor SB203580 or genetic ablation of the map2k6 gene could significantly alleviate combined LAP and DOX exposure-induced cardiotoxicity. Thus, we provided both pharmacologic and genetic evidence to suggest that inhibiting MAPK signaling could exert cardioprotective effects. These findings have implications for understanding the potential cardiotoxicity induced by LAP and DOX combinational chemotherapy in the fetus during pregnancy, which could be leveraged for the development of new therapeutic strategies.
وصف الملف: electronic resource
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3دورية أكاديمية
المؤلفون: Yuanchao Sun, Peng Sun, Yanting Hu, Liying Shan, Qi Geng, Yutian Gong, Haitao Fan, Teng Zhang, Yang Zhou
المصدر: BMC Biology, Vol 20, Iss 1, Pp 1-16 (2022)
مصطلحات موضوعية: Male infertility, Prediabetes, 16S-rDNA, Metabolome, Sphingosine, Melatonin, Biology (General), QH301-705.5
الوصف: Abstract Background Men with prediabetes often exhibit concomitant low-quality sperm production or even infertility, problems which urgently require improved therapeutic options. In this study, we have established a sheep model of diet-induced prediabetes that is associated with spermatogenic defects and have explored the possible underlying metabolic causes. Results We compared male sheep fed a normal diet with those in which prediabetes was induced by a rich diet and with a third group in which the rich diet was supplemented by melatonin. Only the rich diet group had symptoms of prediabetes, and in these sheep, we found impaired spermatogenesis characterized by a block in the development of round spermatids and an increased quantity of testicular apoptotic cells. Comparing the gut microbiomes and intestinal digest metabolomes of the three groups revealed a distinctive difference in the taxonomic composition of the microbiota in prediabetic sheep, and an altered metabolome, whose most significant feature was altered sphingosine metabolism; elevated sphingosine was also found in blood and testes. Administration of melatonin alleviated the symptoms of prediabetes, including those of impaired spermatogenesis, while restoring a more normal microbiota and metabolic levels of sphingosine. Fecal microbiota transplantation from prediabetic sheep induced elevated sphingosine levels and impaired spermatogenesis in recipient mice, indicating a causal role of gut microbiota in these phenotypes. Conclusions Our results point to a key role of sphingosine in the disruption of spermatogenesis in prediabetic sheep and suggest it could be a useful disease marker; furthermore, melatonin represents a potential prebiotic agent for the treatment of male infertility caused by prediabetes.
وصف الملف: electronic resource
Relation: https://doaj.org/toc/1741-7007
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4دورية أكاديمية
المؤلفون: Jianhua Chen, Ping Yang, Qian Zhang, Ruirui Chen, Peng Wang, Benxiu Liu, Wensheng Sun, Xuemin Jian, Siying Xiang, Juan Zhou, Ningning Li, Ke Wang, Chengwen Gao, Yanqin Wen, Chuanhong Wu, Jinmai Zhang, Yalin Zhao, Qiangzhen Yang, Meihang Li, Robert Stewart, Yuanchao Sun, Dun Pan, Yujuan Niu, Zhuo Wang, Yifeng Xu, Xingwang Li, Lin He, Zhiqiang Li, Yongyong Shi
المصدر: Translational Psychiatry, Vol 11, Iss 1, Pp 1-10 (2021)
مصطلحات موضوعية: Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571
الوصف: Abstract Background Clozapine is considered to be the most effective antipsychotic medication for schizophrenia. However, it is associated with several adverse effects such as leukopenia, and the underlying mechanism has not yet been fully elucidated. The authors performed a genome-wide association study (GWAS) in a Chinese population to identify genetic markers for clozapine-induced leukopenia (CIL) and clozapine-induced neutropenia (CIN). Methods A total of 1879 patients (225 CIL cases, including 43 CIN cases, and 1,654 controls) of Chinese descent were included. Data from common and rare single nucleotide polymorphisms (SNPs) were tested for association. The authors also performed a trans-ancestry meta-analysis with GWAS results of European individuals from the Clozapine-Induced Agranulocytosis Consortium (CIAC). Results The authors identified several novel loci reaching the threshold of genome-wide significance level (P
وصف الملف: electronic resource
Relation: https://doaj.org/toc/2158-3188
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5دورية أكاديمية
المؤلفون: Yujuan Niu, Yuanchao Sun, Yuting Liu, Ke Du, Xiaolei Xu, Yonghe Ding
المصدر: International Journal of Molecular Sciences, Vol 24, Iss 4, p 4106 (2023)
مصطلحات موضوعية: arrhythmogenic cardiomyopathy, zebrafish, genetic, disease model, Biology (General), QH301-705.5, Chemistry, QD1-999
الوصف: Arrhythmogenic cardiomyopathy (ACM) is largely an autosomal dominant genetic disorder manifesting fibrofatty infiltration and ventricular arrhythmia with predominantly right ventricular involvement. ACM is one of the major conditions associated with an increased risk of sudden cardiac death, most notably in young individuals and athletes. ACM has strong genetic determinants, and genetic variants in more than 25 genes have been identified to be associated with ACM, accounting for approximately 60% of ACM cases. Genetic studies of ACM in vertebrate animal models such as zebrafish (Danio rerio), which are highly amenable to large-scale genetic and drug screenings, offer unique opportunities to identify and functionally assess new genetic variants associated with ACM and to dissect the underlying molecular and cellular mechanisms at the whole-organism level. Here, we summarize key genes implicated in ACM. We discuss the use of zebrafish models, categorized according to gene manipulation approaches, such as gene knockdown, gene knock-out, transgenic overexpression, and CRISPR/Cas9-mediated knock-in, to study the genetic underpinning and mechanism of ACM. Information gained from genetic and pharmacogenomic studies in such animal models can not only increase our understanding of the pathophysiology of disease progression, but also guide disease diagnosis, prognosis, and the development of innovative therapeutic strategies.
وصف الملف: electronic resource
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6دورية أكاديمية
المؤلفون: Jianhua Chen, Ping Yang, Qian Zhang, Ruirui Chen, Peng Wang, Benxiu Liu, Wensheng Sun, Xuemin Jian, Siying Xiang, Juan Zhou, Ningning Li, Ke Wang, Chengwen Gao, Yanqin Wen, Chuanhong Wu, Jinmai Zhang, Yalin Zhao, Qiangzhen Yang, Meihang Li, Robert Stewart, Yuanchao Sun, Dun Pan, Yujuan Niu, Zhuo Wang, Yifeng Xu, Xingwang Li, Lin He, Zhiqiang Li, Yongyong Shi
المصدر: Translational Psychiatry, Vol 11, Iss 1, Pp 1-1 (2021)
مصطلحات موضوعية: Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571
وصف الملف: electronic resource
Relation: https://doaj.org/toc/2158-3188
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7
المؤلفون: Yan Zhang, Yuanchao Sun, Xia Dong, Qiang-Song Wang, Dunwan Zhu, Lin Mei, Husheng Yan, Feng Lv
المصدر: ACS Nano. 16:6359-6371
مصطلحات موضوعية: Blood Platelets, General Engineering, General Physics and Astronomy, Hyperthermia, Induced, Phototherapy, Mice, Drug Liberation, Drug Delivery Systems, Doxorubicin, Cell Line, Tumor, Neoplasms, Animals, Nanoparticles, General Materials Science
الوصف: Controllable and visible delivery of therapeutic agents is critical for tumor precise therapy. Tumor targeting and deep penetration of therapeutic agents are still challenging issues for controllable delivery. Visible drug delivery with imaging navigation can optimize the treatment window for personalized medicine. Herein, a biomimetic platelet intelligent vehicle with navigation (IRDNP-PLT) was developed to achieve controllable and visible delivery with a navigation system, a driving system, and a loading system. The platelets acted as engines and drug repositories to exert the target driving and delivery functions. The fluorescent photothermal agent IR-820 was introduced in the platform to offer an imaging navigation for the intelligent platelet vehicle in addition to photothermal therapy. The nanodrug-loaded platelets enabled efficient drug loading and controlled release of the therapeutic payload by encapsulating photothermal-/pH-sensitive chemotherapeutic nanoparticles (PDA@Dox NPs). Iniin vivo/iexperiments on 4T1 tumor-bearing mice models, IRDNP-PLT performed well in tumor targeting and showed excellent therapeutic efficacy and tumor recurrence prevention ability. The intelligent platelet vehicle achieved the functions of tumor targeting and deep penetration, fluorescence imaging guidance, photocontrolled drug release, and chemo-photothermal combination therapy, suggesting the advancement for tumor precise delivery and efficient therapy.
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8
المؤلفون: Kaihui, Chang, Nan, Zeng, Yonghe, Ding, Xiangzhong, Zhao, Chengwen, Gao, Yafang, Li, Haoxu, Wang, Xiaoyu, Liu, Yujuan, Niu, Yuanchao, Sun, Teng, Li, Yongyong, Shi, Chuanhong, Wu, Zhiqiang, Li
المصدر: Foodfunction. 13(24)
مصطلحات موضوعية: Oxidative Stress, Animals, Neurotoxicity Syndromes, Acrolein, Zebrafish
الوصف: Toxicology studies provide a reliable dose range for the use of compounds. Zebrafish show unique advantages in toxicology research. Cinnamaldehyde (Cin) is one of the main active compounds isolated from
URL الوصول: https://explore.openaire.eu/search/publication?articleId=pmid________::97c65447407db528f6aeedaf0574d9be
https://pubmed.ncbi.nlm.nih.gov/36449017 -
9
المؤلفون: Changgui Li, Lin Han, Can Wang, Zhaowei Zhou, Chuanhong Wu, Jie Lu, Lingling Cui, Zhen Liu, Hailong Li, Xinde Li, Jianhua Chen, Aichang Ji, Xuefeng Wang, Yuwei He, Chengwen Gao, Xiaopeng Ji, Xuan Yuan, Juan Zhou, Ke Wang, Qian Zhang, Wenyan Sun, Hui Zhang, Wei Ren, Dun Pan, Jinmai Zhang, Qiangzhen Yang, Yanqin Wen, Meihang Li, Yonghe Ding, Yuanchao Sun, Yujuan Niu, Zhuo Wang, Yongyong Shi, Zhiqiang Li
المصدر: SSRN Electronic Journal.
مصطلحات موضوعية: History, Polymers and Plastics, Business and International Management, Industrial and Manufacturing Engineering
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_________::84feda12d3552f168b0ea5c261fd030a
https://doi.org/10.2139/ssrn.4106761 -
10
المؤلفون: Meihang Li, Yongyong Shi, Haiying Ma, Yujuan Niu, Yuanchao Sun, Zhiqiang Li, Yonghe Ding
المصدر: Poultry Science. 98:6333-6339
مصطلحات موضوعية: Serotype, Picornaviridae Infections, Virulence, Mortality rate, medicine.medical_treatment, Spleen, Immunosuppression, General Medicine, Biology, Virology, Hepatitis Virus, Duck, Pathogenesis, Vaccination, Ducks, medicine.anatomical_structure, Immune system, Hepatitis, Viral, Animal, medicine, Animals, RNA, Viral, Animal Science and Zoology, Pancreas, Poultry Diseases
الوصف: Duck hepatitis A virus (DHAV) is one of the pathogens that cause fatal duck viral hepatitis (DVH) in ducklings, which is an acute and contagious disease with a high mortality rate. Despite a continuing official duck vaccination program, DHAV infection remains a major threat to the duck industry. Considerable changes were observed in the epidemiology of DHAV-1/-3 in China over time. Therefore, comparing the pathogenicity of different DHAV serotypes can provide a theoretical basis for the diagnosis and prevention of DVH. In this study, we systematically investigated the effects of infection with DHAV-1/-3 field strains on clinical signs, gross lesions, histopathological changes, viral RNA detection, enzymatic systems, and metabolite concentrations. The results demonstrated that the major macroscopic and microscopic lesions in ducks infected with DHAV-1/-3 in the liver, brain, spleen, pancreas, and kidneys exhibited no significant differences. After 24 h of infection, DHAV quickly appeared in blood and major organs. Significant changes in clinical chemical markers together with histopathological lesions and viral RNA detection indicated that the liver is the major target organ for both viruses, resulting in impaired of liver integrity and function. In addition, we found that both viruses were able to invade both central and peripheral immune organs. Also lipase plasma activity was substantially affected by DHAV-1/-3, indicating that the integrity and function of the pancreas was compromised. However, there was no significant difference in pathogenicity between DHAV-1 and -3. The results of this study provide new insights into the pathogenesis of DHAV-1/3, two viruses that cause serious depression, metabolic disorders, and immunosuppression.
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::767fcf342427d97b9e8e5bdbe738bd13
https://doi.org/10.3382/ps/pez455
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