المساهمون: University/Department: Universitat Politècnica de València. Departamento de Producción Vegetal - Departament de Producció Vegetal

مرشدي الرسالة: Abad Campos, María Paloma, PEREZ Y SIERRA, ANA MARIA

مصطلحات موضوعية: Phytophthora, amplicon pyrosequencing, metabarcoding, environmental DNA, PRODUCCION VEGETAL

URL الوصول: http://hdl.handle.net/10251/90644

المؤلفون: Ziganshin, Ayrat M., Wintsche, Babett, Seifert, Jana, Carstensen, Martina, Born, Jens, Kleinsteuber, Sabine^Aff2

المصدر: Applied Microbiology and Biotechnology. 103(9):3915-3929

المؤلفون: Zhen-yu Zhang, Muhammad Waqar Ali, Hafiz Sohaib Ahmed Saqib, Sheng-xuan Liu, Xin Yang, Qin Li, Hongyu Zhang

المصدر: Frontiers in Microbiology, Vol 11 (2020)

مصطلحات موضوعية: Panonychus citri (McGregor), spider mites, developmental stages, 16S rDNA amplicon pyrosequencing, dynamics of bacterial community, bacterial metabolism, Microbiology, QR1-502

الوصف: As one of the most detrimental citrus pests worldwide, the citrus red mite, Panonychus citri (McGregor), shows extraordinary fecundity, polyphagia, and acaricide resistance, which may be influenced by microbes as other arthropod pests. However, the community structure and physiological function of microbes in P. citri are still largely unknown. Here, the high-throughput sequencing of 16S rDNA amplicons was employed to identify and compare the profile of bacterial communities across the larva, protonymph, deutonymph, and adult stages of P. citri. We observed a dominance of phylums Proteobacteria and Firmicutes, and classes α-, γ-, β-Proteobacteria and Bacilli in the bacterial communities across the host lifespan. Based on the dynamic analysis of the bacterial community structure, a significant shift pattern between the immature (larva, protonymph, and deutonymph) and adult stages was observed. Accordingly, among the major families (and corresponding genera), although the relative abundances of Pseudomonadaceae (Pseudomonas), Moraxellaceae (Acinetobacter), and Sphingobacteriaceae (Sphingobacterium) were consistent in larva to deutonymph stages, they were significantly increased to 30.18 ± 8.76% (30.16 ± 8.75%), 20.78 ± 10.86% (18.80 ± 10.84%), and 11.71 ± 5.49% (11.68 ± 5.48%), respectively, in adult stage, which implied the important function of these bacteria on the adults’ physiology. Actually, the functional prediction of bacterial communities and Spearman correlation analysis further confirm that these bacteria had positively correlations with the pathway of “lipid metabolism” (including eight sublevel pathways) and “metabolism of cofactors and vitamins” (including five sublevel pathways), which all only increased in adult stages. In addition, the bacterial communities were eliminated by using broad-spectrum antibiotics, streptomycin, which significantly suppressed the survival and oviposition of P. citri. Overall, we not only confirmed the physiological effects of bacteria community on the vitality and fecundity of adult hosts, but also revealed the shift pattern of bacterial community structures across the life stages and demonstrated the co-enhancements of specific bacterial groups and bacterial functions in nutritional metabolism in P. citri. This study sheds light on basic information about the mutualism between spider mites and bacteria, which may be useful in shaping the next generation of control strategies for spider mite pests, especially P. citri.

وصف الملف: electronic resource

Relation: https://www.frontiersin.org/article/10.3389/fmicb.2020.01620/full; https://doaj.org/toc/1664-302X

URL الوصول: https://doaj.org/article/d0c3c48b6640404299fedaa4937c5cd1

المساهمون: Hazen, T

المصدر: PLoS One; 6; 4; Other Information: Journal Publication Date: 2011

وصف الملف: Medium: ED; Size: e19306

URL الوصول: http://www.osti.gov/scitech/servlets/purl/1050668

المؤلفون: Gonzalez-Silva, Blanca M., Rønning, Are J., Andreassen, Ingrid K., Bakke, Ingrid, Cervantes, Francisco J., Østgaard, Kjetill, Vadstein, Olav

المصدر: Applied Microbiology and Biotechnology. June 2017 101(12):5149-5162

المؤلفون: Schmitt, Susanne^{Aff3, Aff4}, Hentschel, Ute^Aff3, Taylor, Michael W.^Aff4

المساهمون: Martens, Koen, editor, Maldonado, Manuel, editor^Aff1, Turon, Xavier, editor^Aff2, Becerro, Mikel, editor^Aff2, Jesús Uriz, Maria, editor^Aff2

المصدر: Ancient Animals, New Challenges : Developments in Sponge Research. 219:341-351

المؤلفون: Morrow, J. L., Frommer, M., Shearman, D. C. A., Riegler, M.

المصدر: Microbial Ecology. August 2015 70(2):498-508

لا يتم عرض هذه النتيجة على الضيوف.
تسجيل الدخول للوصول الكامل.

المؤلفون: Walker, Alesia, Pfitzner, Barbara, Neschen, Susanne, Kahle, Melanie, Harir, Mourad, Lucio, Marianna, Moritz, Franco, Tziotis, Dimitrios, Witting, Michael, Rothballer, Michael, Engel, Marion, Schmid, Michael, Endesfelder, David, Klingenspor, Martin, Rattei, Thomas, Castell, Wolfgang zu, de Angelis, Martin Hrabé, Hartmann, Anton, Schmitt-Kopplin, Philippe^{Aff1, Aff8, IDismej201479_cor19}

المصدر: The ISME Journal: Multidisciplinary Journal of Microbial Ecology. 8(12):2380-2396

المؤلفون: Daniela eKlein-Jöbstl, Elisa eSchornsteiner, Evelyne eMann, Martin eWagner, Marc eDrillich, Stephan eSchmitz-Esser

المصدر: Frontiers in Microbiology, Vol 5 (2014)

مصطلحات موضوعية: early development, calf, fecal bacterial community, 16S rRNA amplicon pyrosequencing, Simmental breed, Microbiology, QR1-502

الوصف: From birth to the time after weaning the gastrointestinal microbiota of calves must develop into a stable, autochthonous community accompanied by pivotal changes of anatomy and physiology of the gastrointestinal tract. The aim of this pilot study was to examine the fecal microbiota of six Simmental dairy calves to investigate time-dependent dynamics of the microbial community. Calves were followed up from birth until after weaning according to characteristic timepoints during physiological development of the gastrointestinal tract. Pyrosequencing of 16S rRNA gene amplicons from 35 samples yielded 253,528 reads clustering into 5,410 operational taxonomic units based on 0.03 16S rRNA distance. Operational taxonomic units were assigned to 296 genera and 17 phyla with Bacteroidetes, Firmicutes and Proteobacteria being most abundant. An age-dependent increasing diversity and species richness was observed. Highest similarities between fecal microbial communities were found around weaning compared with timepoints from birth to the middle of the milk feeding period. Principal coordinate analysis revealed a high variance particularly in samples taken at the middle of the milk feeding period (at the age of approximately 40 days) compared to earlier timepoints, confirming a unique individual development of the fecal microbiota of each calf. This study provides first deep insights into the composition of the fecal microbiota of Simmental dairy calves and might be a basis for future more detailed studies.

وصف الملف: electronic resource

Relation: http://journal.frontiersin.org/Journal/10.3389/fmicb.2014.00622/full; https://doaj.org/toc/1664-302X

URL الوصول: https://doaj.org/article/8bc7f9ed8db948ffad99f4b6e9042517