المؤلفون: Tripathi, Deepika, Meena, Ram Prasad, Pandey-Rai, Shashi^{Aff1, IDs12298021010467_cor3}

المصدر: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology. 27(8):1823-1835

المؤلفون: A. O. Savych, S. M. Marchyshyn, N. M. Ostrovsky, Y. B. Laryanovska

المصدر: Фармацевтичний журнал, Vol 5, Pp 92-100 (2018)

مصطلحات موضوعية: antidiabetic plant collection, diabetes mellitus, histological study, pancreatic β-cells, Therapeutics. Pharmacology, RM1-950, Pharmacy and materia medica, RS1-441

الوصف: Diabetes mellitus is a global problem today, because there is a sharp increase the number of patients in the world each year. Severe complications of diabetes are very dangerous because it can lead to disability of patients and high mortality. The aim of our work was to study the protective effect of antidiabetic plant collection on the pancreas in rats with diabetes type 2, caused by the introduction of dexamethasone. The research materials were pancreatic tissue of rats with experimental diabetes that received the decoction (1:10) of antidiabetic plant collection (witch includes Equiseti arvensis herba, Sambuci flores, Inulae rhizomata et radices, Hyperici herba, Tiliae flores, Polygoni avicularis herba, Myrtilli folium, Urticae folia) in treatment and preventive mode. The model of diabetes mellitus was induced by administration of dexamethasone 0.125 ml/kg for 15 days. Decoction of antidiabetic plant collection in doses 6 ml/kg, 9 ml/kg and 12 ml/kg reduces diabetogenic properties of dexamethasone when administered simultaneously. The insular tissue increases absorbance pancreatic islets, to some extent restored the normal percentage distribution of the islets in size, histologically visualized the increase more high-grade cells. The study found that antidiabetic plant collection reduces destructive-dystrophic changes of insular apparatus in rats with diabetes type 2. Antidiabetic effect of the studied plant collection has dose-dependent nature. The most effective conditional-effective doses are 9 and 12 ml/kg.

وصف الملف: electronic resource

Relation: https://pharmj.org.ua/index.php/journal/article/view/91/150; https://doaj.org/toc/0367-3057; https://doaj.org/toc/2617-9628

URL الوصول: https://doaj.org/article/b5569dae942d4723ba385a59cf2d033a

المؤلفون: Renate Haselgrübler, author¹, Flora Stübl, author¹, Verena Stadlbauer, author^{1, 2}, Peter Lanzerstorfer, author¹, Julian Weghuber, author^{1, 2}

المصدر: Journal of Visualized Experiments. (134)

الإتاحة: http://www.jove.com/video/57237

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المؤلفون: Pepato, M.T., Folgado, V.B.B., Kettelhut, I.C., Brunetti, I.L.

المصدر: Brazilian Journal of Medical and Biological Research. March 2001 34(3)

مصطلحات موضوعية: antidiabetic plant, hyperglycemia, diabetic rats, Eugenia jambolana

الوصف: Streptozotocin-diabetic rats were treated for 17 days with a decoction of Eugenia jambolana (Myrtaceae) leaves (15%, w/v) as a substitute for water. Body weight, food and fluid intake, urine volume, glycemia, urinary glucose and urea were evaluated every 5 days. The animals were sacrificed by decapitation and blood samples collected for the determination of glycemia, serum cholesterol, HDL-cholesterol, triglycerides and angiotensin-converting enzyme. The weight of adipose and muscle tissues was also determined. There were no statistically significant differences between treated and untreated rats for any of the biochemical or physiological parameters. We conclude that, at least in this experimental model, Eugenia jambolana leaf decoction has no antidiabetic activity.

وصف الملف: text/html

URL الوصول: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000300014

المؤلفون: Deepika, Tripathi, Ram Prasad, Meena, Shashi, Pandey-Rai

المصدر: Physiology and Molecular Biology of Plants

مصطلحات موضوعية: Secondary metabolites, Antidiabetic plant, UV-B radiation, In-vitro grown plantlets, Abiotic elicitor, Research Article, Antioxidative defense system

الوصف: Accumulation of secondary metabolites is a key process in the growth and development of plants under different biotic/abiotic constraints. Many studies highlighted the regulatory potential of UV-B treatment towards the secondary metabolism of plants. In the present study, we examined the impact of UV-B on the physiology and secondary metabolism of Withania coagulans, which is an important ayurvedic plant with high anti-diabetic potential. Results showed that in-vitro UV-B exposure negatively influenced chlorophyll content and photosynthetic machinery. However, Fv/Fm ratio was found non-significantly altered up to 3 h UV-B exposure. The maximum lipid peroxidation level was recorded with 46.8% higher malondialdehyde content in the plants supplemented with 5 h UV-B radiation, that was indicated the oxidative stress in W. coagulans. Conversely, UV-B treatment significantly increased the plant's stress protective compounds like carotenoids, anthocyanin, phenol and proline, in W. coagulans. Free radical scavenging activity was also significantly increased ~ 18% than the control with 3 h UV-B treatment. The maximum antioxidative enzymes activities were observed with the short-term (up to 3 h) UV-B treatment. Specifically, UV-B radiation exposure significantly increased the content of withaferin A and withanolide A in W. coagulans with maximum 1.38 and 3.42-folds, respectively. Additionally, withanolides biosynthesis related genes transcript levels were found over-expressed under the response of UV-B elicitation. The acquired results suggested that short-term UV-B supplementation triggers secondary metabolism along with combating oxidative stress via improving the antioxidative defense system in W. coagulans. Also, UV-B can be used as an efficient abiotic elicitor to increase pharmaceutical compounds (withanolides) production. Supplementary Information The online version contains supplementary material available at 10.1007/s12298-021-01046-7.

URL الوصول: https://explore.openaire.eu/search/publication?articleId=pmid________::43b082693d50c8e0a233e6c33f1e0c53
https://pubmed.ncbi.nlm.nih.gov/34393390

المؤلفون: N. M. Ostrovsky, S. M. Marchyshyn, A. O. Savych, Y. B. Laryanovska

المصدر: Фармацевтичний журнал, Vol 5, Pp 92-100 (2018)

مصطلحات موضوعية: geography, geography.geographical_feature_category, pancreatic β-cells, Pancreatic tissue, business.industry, Pancreatic islets, histological study, lcsh:RM1-950, antidiabetic plant collection, lcsh:RS1-441, Decoction, Plant collection, Pharmacology, medicine.disease, Islet, lcsh:Pharmacy and materia medica, medicine.anatomical_structure, lcsh:Therapeutics. Pharmacology, Diabetes mellitus, diabetes mellitus, medicine, Pancreas, business, Dexamethasone, medicine.drug

الوصف: Diabetes mellitus is a global problem today, because there is a sharp increase the number of patients in the world each year. Severe complications of diabetes are very dangerous because it can lead to disability of patients and high mortality. The aim of our work was to study the protective effect of antidiabetic plant collection on the pancreas in rats with diabetes type 2, caused by the introduction of dexamethasone. The research materials were pancreatic tissue of rats with experimental diabetes that received the decoction (1:10) of antidiabetic plant collection (witch includes Equiseti arvensis herba, Sambuci flores, Inulae rhizomata et radices, Hyperici herba, Tiliae flores, Polygoni avicularis herba, Myrtilli folium, Urticae folia) in treatment and preventive mode. The model of diabetes mellitus was induced by administration of dexamethasone 0.125 ml/kg for 15 days. Decoction of antidiabetic plant collection in doses 6 ml/kg, 9 ml/kg and 12 ml/kg reduces diabetogenic properties of dexamethasone when administered simultaneously. The insular tissue increases absorbance pancreatic islets, to some extent restored the normal percentage distribution of the islets in size, histologically visualized the increase more high-grade cells. The study found that antidiabetic plant collection reduces destructive-dystrophic changes of insular apparatus in rats with diabetes type 2. Antidiabetic effect of the studied plant collection has dose-dependent nature. The most effective conditional-effective doses are 9 and 12 ml/kg.

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ef72d4ed01c81ac5323fcf58011e3b1c
https://pharmj.org.ua/index.php/journal/article/view/91/150

المؤلفون: M.T. Pepato, V.B.B. Folgado, I.C. Kettelhut, I.L. Brunetti

المصدر: Brazilian Journal of Medical and Biological Research, Vol 34, Iss 3, Pp 389-395 (2001)

مصطلحات موضوعية: antidiabetic plant, hyperglycemia, diabetic rats, Eugenia jambolana, Medicine (General), R5-920, Biology (General), QH301-705.5

الوصف: Streptozotocin-diabetic rats were treated for 17 days with a decoction of Eugenia jambolana (Myrtaceae) leaves (15%, w/v) as a substitute for water. Body weight, food and fluid intake, urine volume, glycemia, urinary glucose and urea were evaluated every 5 days. The animals were sacrificed by decapitation and blood samples collected for the determination of glycemia, serum cholesterol, HDL-cholesterol, triglycerides and angiotensin-converting enzyme. The weight of adipose and muscle tissues was also determined. There were no statistically significant differences between treated and untreated rats for any of the biochemical or physiological parameters. We conclude that, at least in this experimental model, Eugenia jambolana leaf decoction has no antidiabetic activity.

وصف الملف: electronic resource

Relation: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000300014; https://doaj.org/toc/0100-879X; https://doaj.org/toc/1414-431X

URL الوصول: https://doaj.org/article/e18c32b77ac940d28b675e1b2341c9b5

المؤلفون: Hoa, Nguyen Khanh

المساهمون: Karolinska Institutet, Department of Molecular Medicine, Publisher, Karolinska Institutet, Institutionen för molekylär medicin, Publisher

مصطلحات موضوعية: MEDICAL AND HEALTH SCIENCES, MEDICIN OCH HÄLSOVETENSKAP, G-protein, type 2 diabetes, phanoside, antidiabetic plant, hypoglycemic agent, insulin secretion, pancreatic islets, Gynostemma pentaphyllum, Anemarrhena asphodeloides, Angiopteris evecta

الوصف: The prevalence of type 2 diabetes mellitus is increasing in Vietnam as well as in other developing countries (China, Indian subcontinent, and Africa). Searching for hypoglycemic agents with origin from domestic herbals was considered as a useful way to find novel therapy of the disease.After treatment i.p. or orally of normal mice with extract of Anemarrhena asphodeloides (A.a.), Angiopteris evecta (A.e.) and Gynostemma pentaphyllum (G.p.), blood glucose levels of the mice were decreased. All of those 3 extracts also suppressed the rise in blood glucose in normal mice during a glucose tolerance test.At both 3.3 and 16.7 mM glucose, 2, 4 and 8 mg/ml Anemarrhena asphodeloides (A.a. or TH2) increased the insulin release of Wistar (W) and Goto- Kakizaki (GK) rat islets. In perifusions of islets, A.a. also increased insulin secretion that returned to basal levels when A.a. was omitted from the perifusate. Thus, ethanol extract of the roots of A.a. contains a substance, TH2, that stimulates insulin secretion from islets of normal W and GK rats. The mechanism behind TH2-stimulated insulin secretion involves an effect on the exocytotic machinery of the B-cell, mediated via pertussis toxin-sensitive Ge-proteins.G.p. extract had a hypoglycemic effect in rats and mice. We have isolated the active compound, phanoside, a gypenoside with molecular mass of 914.5 Da. When given orally to rats, phanoside (40 and 80 mg/kg) improved glucose tolerance and enhanced plasma insulin levels. Phanoside stimulated insulin release at 3.3 and 16.7 mM glucose from isolated rat pancreatic islets of both W and GK rats. Interestingly, B- cell sensitivity to phanoside is higher at 16.7 mM than at 3.3 mM glucose, since significant insulin responses were observed with phanoside between 31.25 and 125 μM only at the high glucose levels.When W rat islets were incubated at 3.3 mM glucose with 150 μM phanoside and 0.25 mM diazoxide to keep K-ATP channels open, insulin secretion was similar to that in islets incubated in 150 μM phanoside alone. At 16.7 mM glucose, phanoside-stimulated insulin secretion was reduced in the presence of 0.25 mM diazoxide. In W islets depolarized by 50 mM KCl and with diazoxide, phanoside stimulated insulin release 2-fold at 3.3 mM glucose but did not further increase the release at 16.7 mM glucose. When using nimodipine to block L-type Ca2+ channels in B-cells, phanoside-induced insulin secretion was unaffected at 3.3 mM glucose but decreased at 16.7 mM glucose. In perifusion of islets, phanoside (75 and 150 μM) dose- dependently increased insulin secretion that returned to basal levels when phanoside was omitted. Thus, the effect of phanoside seems to be exerted distal to K-ATP channels and L-type Ca2+ channels that is on the exocytotic machinery of the B-cells.In conclusion, from 8 Vietnamese herbal drugs, we have found 3 extracts which decreased blood glucose of the mice. Two of them (A.a and G.p. extract) stimulated insulin secretion from rat islets. Ethanol extract of A.a. (TH2) stimulated insulin secretion by an effect on the exocytotic machinery of the B cell mediated via pertussis toxin sensitive Ge-protein. From G.p. we islolated a novel substance, phanoside, that directly stimulates the exocytosis of insulin.

وصف الملف: electronic

URL الوصول: http://hdl.handle.net/10616/39365

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