المؤلفون: Xi Y; Department of Urology, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong 510000, China., Feng Z; Department of Urology, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong 510000, China., Xia T; Department of Urology, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong 510000, China., Hong Y; Department of Urology, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong 510000, China., Wu J; Department of Urology, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong 510000, China., Chen J; Department of Urology, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong 510000, China., Ge Y; Department of Urology, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong 510000, China; Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230000, China. Electronic address: geylong3@mail2.sysu.edu.cn., Xiao H; Department of Urology, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, Guangdong 510000, China. Electronic address: xiaohjun@mail.sysu.edu.cn.

المصدر: Life sciences [Life Sci] 2024 Jul 01; Vol. 348, pp. 122694. Date of Electronic Publication: 2024 May 06.

نوع المنشور: Journal Article

بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0375521 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0631 (Electronic) Linking ISSN: 00243205 NLM ISO Abbreviation: Life Sci Subsets: MEDLINE

مواضيع طبية MeSH: Apoptosis*/drug effects , Oxidative Stress*/drug effects , Rats, Sprague-Dawley* , Erectile Dysfunction*/drug therapy , Erectile Dysfunction*/metabolism , Erectile Dysfunction*/etiology , Penis*/drug effects , Penis*/innervation , Penis*/pathology , Caveolin 1*/metabolism , Mitochondria*/drug effects , Mitochondria*/metabolism , Penile Erection*/drug effects , Myocytes, Smooth Muscle*/drug effects , Myocytes, Smooth Muscle*/metabolism, Animals ; Male ; Rats ; Peptides/pharmacology

مستخلص: Aim: Increased corpus cavernosum smooth muscle cells (CCSMCs) apoptosis in the penis due to cavernous nerve injury (CNI) is a crucial contributor to erectile dysfunction (ED). Caveolin-1 scaffolding domain (CSD)-derived peptide has been found to exert potential antiapoptotic properties. However, whether CSD peptide can alleviate CCSMCs apoptosis and ED in CNI rats remains unknown. The study aimed to determine whether CSD peptide can improve bilateral CNI-induced ED (BCNI-ED) by enhancing the antiapoptotic processes of CCSMCs.
Main Methods: Fifteen 10-week-old male Sprague-Dawley (SD) rats were randomly classified into three groups: sham surgery (Sham) group and BCNI groups that underwent saline or CSD peptide treatment respectively. At 3 weeks postoperatively, erectile function was assessed and the penis tissue was histologically examined. Furthermore, an in vitro model of CCSMCs apoptosis was established using transforming growth factor-beta 1 (TGF-β1) to investigate the mechanism of CSD peptide in treating BCNI-ED.
Key Findings: In BCNI rats, CSD peptide significantly prevented ED and decreased oxidative stress, the Bax/Bcl-2 ratio, and the levels of caspase3. TGF-β1-treated CCSMCs exhibited severe oxidative stress, mitochondrial dysfunction, and apoptosis. However, CSD peptide partially reversed these alterations.
Significance: Exogenous CSD peptide could improve BCNI-ED by inhibiting oxidative stress, the Bax/Bcl-2 ratio, and caspase3 expression in penile tissue. The underlying mechanism might involve the regulatory effects of CSD peptide on oxidative stress, mitochondrial dysfunction, and apoptosis of CCSMCs following CNI. This study highlights CSD peptide as an effective therapy for post-radical prostatectomy ED (pRP-ED).
Competing Interests: Declaration of competing interest The authors declare no conflict of interest.
(Copyright © 2024 Elsevier Inc. All rights reserved.)

المؤلفون: Zhu S; Andrology Laboratory, West China Hospital, Sichuan University, Chengdu, China.; Department of Urology, West China Hospital, Sichuan University, Chengdu, China., Xiong Y; Andrology Laboratory, West China Hospital, Sichuan University, Chengdu, China.; Department of Urology, West China Hospital, Sichuan University, Chengdu, China., Yu B; Andrology Laboratory, West China Hospital, Sichuan University, Chengdu, China.; Department of Urology, West China Hospital, Sichuan University, Chengdu, China.; Department of Urology, Ningbo Medical Center, Lihuili Hospital, Ningbo, China., Wang H; Andrology Laboratory, West China Hospital, Sichuan University, Chengdu, China.; Department of Urology, West China Hospital, Sichuan University, Chengdu, China., Zhang F; Andrology Laboratory, West China Hospital, Sichuan University, Chengdu, China.; Department of Urology, West China Hospital, Sichuan University, Chengdu, China., Wu C; Andrology Laboratory, West China Hospital, Sichuan University, Chengdu, China., Qin F; Andrology Laboratory, West China Hospital, Sichuan University, Chengdu, China., Yuan J; Andrology Laboratory, West China Hospital, Sichuan University, Chengdu, China. jiuhongyuan2107@163.com.; Department of Urology, West China Hospital, Sichuan University, Chengdu, China. jiuhongyuan2107@163.com.

المصدر: International journal of impotence research [Int J Impot Res] 2024 Jun; Vol. 36 (4), pp. 430-436. Date of Electronic Publication: 2023 Feb 22.

نوع المنشور: Journal Article

بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 9007383 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1476-5489 (Electronic) Linking ISSN: 09559930 NLM ISO Abbreviation: Int J Impot Res Subsets: MEDLINE

مواضيع طبية MeSH: Autophagy*/drug effects , Apoptosis*/drug effects , Rats, Sprague-Dawley* , Cholecalciferol*/pharmacology , Erectile Dysfunction*/drug therapy , Erectile Dysfunction*/etiology , Penis*/innervation , Penis*/drug effects , Penile Erection*/drug effects, Animals ; Male ; Rats ; Disease Models, Animal ; Recovery of Function/drug effects ; Peripheral Nerve Injuries/drug therapy ; Fibrosis

مستخلص: Vitamin D 3 is an important element in improving erectile function. However, the mechanisms of vitamin D 3 remain unknown. Thus, we explored the effect of vitamin D 3 on erectile function recovery after nerve injury in a rat model and investigated its possible molecular mechanisms. Eighteen male Sprague-Dawley rats were used in this study. The rats were randomly divided into three groups: the control, bilateral cavernous nerve crush (BCNC), and BCNC + vitamin D 3 groups. BCNC model was established in rats by surgery. The intracavernosal pressure and the ratio of intracavernosal pressure to mean arterial pressure were utilized to evaluate erectile function. Masson trichrome staining, immunohistochemistry, terminal deoxynucleotidyl transferase-mediated dUTP nick end labelling and western blot analysis were performed on penile tissues to elucidate the molecular mechanism. The results indicated that vitamin D 3 alleviated hypoxia and suppressed the fibrosis signalling pathway by upregulating the expression of eNOS (p = 0.001), nNOS (p = 0.018) and α-SMA (p = 0.025) and downregulating the expression of HIF-1α (p = 0.048) and TGF-β1 (p = 0.034) in BCNC rats. Vitamin D 3 promoted erectile function restoration by enhancing the autophagy process through decreases in the p-mTOR/mTOR ratio (p = 0.02) and p62 (p = 0.001) expression and increases in Beclin1 expression (p = 0.001) and the LC3B/LC3A ratio (p = 0.041). Vitamin D 3 application improved erectile function rehabilitation by suppressing the apoptotic process through decreases in the expression of Bax (p = 0.002) and caspase-3 (p = 0.046) and an increase in the expression of Bcl2 (p = 0.004). Therefore, We concluded that vitamin D 3 improved the erectile function recovery in BCNC rats by alleviating hypoxia and fibrosis, enhancing autophagy and inhibiting apoptosis in the corpus cavernosum.
(© 2023. The Author(s), under exclusive licence to Springer Nature Limited.)

المؤلفون: Kataoka T; Department of Clinical Pharmaceutics, Graduate School of Medical Sciences, Nagoya City University, 1-Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya, 467-8601, Japan. tom.kataoka@aol.com.; Department of Pharmacology, Kataoka's lab, Graduate School of Pharmaceutical Sciences, Chiba Institute of Science, 15-8 Shiomi-cho, Choshi, Chiba, 288-0025, Japan. tom.kataoka@aol.com., Ito H; Department of Hospital Pharmacy, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya, 467-8603, Japan., Mori T; Department of Hospital Pharmacy, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya, 467-8603, Japan., Hotta Y; Department of Hospital Pharmacy, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya, 467-8603, Japan., Sanagawa A; Department of Hospital Pharmacy, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya, 467-8603, Japan., Maeda Y; Department of Hospital Pharmacy, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya, 467-8603, Japan., Furukawa-Hibi Y; Department of Clinical Pharmaceutics, Graduate School of Medical Sciences, Nagoya City University, 1-Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya, 467-8601, Japan.; Department of Hospital Pharmacy, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya, 467-8603, Japan., Kimura K; Department of Clinical Pharmaceutics, Graduate School of Medical Sciences, Nagoya City University, 1-Kawasumi, Mizuho-cho, Mizuho-ku, Nagoya, 467-8601, Japan. kkimura@med.nagoya-cu.ac.jp.; Department of Hospital Pharmacy, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabe-dori, Mizuho-ku, Nagoya, 467-8603, Japan. kkimura@med.nagoya-cu.ac.jp.

المصدر: International journal of impotence research [Int J Impot Res] 2024 Jun; Vol. 36 (4), pp. 437-442. Date of Electronic Publication: 2022 Oct 30.

نوع المنشور: Journal Article

بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 9007383 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1476-5489 (Electronic) Linking ISSN: 09559930 NLM ISO Abbreviation: Int J Impot Res Subsets: MEDLINE

مواضيع طبية MeSH: Testosterone*/pharmacology , Penile Erection*/drug effects , Erectile Dysfunction*/drug therapy , Hormone Replacement Therapy*, Animals ; Male ; Rats ; Nitric Oxide Synthase Type III/metabolism ; Nitric Oxide Synthase Type III/genetics ; Up-Regulation/drug effects ; Rats, Sprague-Dawley ; Intercellular Signaling Peptides and Proteins/genetics ; Orchiectomy ; Androgens/pharmacology ; Penis/drug effects ; Penis/metabolism

مستخلص: We previously showed that castration of rats reduced erectile function over time; when testosterone replacement therapy was started 4 weeks after castration, erectile function improved. In this study, we examined the mechanism of improvement in erectile function following testosterone replacement therapy in rats. Thirty 12-week-old rats were divided into castrated (Cast), castrated with subcutaneous administration of testosterone (Cast + T), and sham (Sham) groups. Erectile function and mRNA and protein expression were evaluated in the rats by using standard methods. To assess erectile function, we measured the intracavernosal pressure, mean arterial pressure, mRNA expression of endothelial growth factors, and protein expression of endothelial nitric oxide synthase (eNOS). The intracavernosal pressure/mean arterial pressure ratio was significantly lower in the Cast group, and testosterone administration significantly improved (P = 0.017). Compared to the Cast group, the Cast+T group exhibited significantly increased mRNA expressions of vascular endothelial growth factor A (VEGF-A), intercellular adhesion molecule 1 (ICAM-1), transforming growth factor-β (TGF-β), nerve growth factor (NGF), α-smooth muscle actin (α-SMA), caveolae associated protein 1 (Cavin-1), Cavin-2, Cavin-3, sirtuin 1 (Sirt-1), sphingosine-1-phosphate 1 (S1P1), S1P2, and S1P3 and eNOS protein expression. Testosterone replacement therapy improved erectile function in castrated rats by increasing growth factors and eNOS protein.
(© 2022. The Author(s), under exclusive licence to Springer Nature Limited.)

المؤلفون: Albersen M; Department of Urology, University Hospitals Leuven, Herestraat 49, 3000 Leuven, Belgium.

المصدر: The journal of sexual medicine [J Sex Med] 2024 May 28; Vol. 21 (6), pp. 509-510.

نوع المنشور: Journal Article; Editorial

بيانات الدورية: Publisher: Oxford University Press Country of Publication: Netherlands NLM ID: 101230693 Publication Model: Print Cited Medium: Internet ISSN: 1743-6109 (Electronic) Linking ISSN: 17436095 NLM ISO Abbreviation: J Sex Med Subsets: MEDLINE

مواضيع طبية MeSH: Erectile Dysfunction*/physiopathology , Fibroblasts*/physiology , Penile Erection*/physiology , Penis*/innervation, Humans ; Male

المؤلفون: Lloyd L; Nature Reviews Urology, . louise.lloyd@nature.com.

المصدر: Nature reviews. Urology [Nat Rev Urol] 2024 Apr; Vol. 21 (4), pp. 193.

نوع المنشور: Journal Article; Comment

بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101500082 Publication Model: Print Cited Medium: Internet ISSN: 1759-4820 (Electronic) Linking ISSN: 17594812 NLM ISO Abbreviation: Nat Rev Urol Subsets: MEDLINE

مواضيع طبية MeSH: Penile Erection* , Penis*, Male ; Humans

المؤلفون: Ryu JK; Department of Urology and Medical Research Center for Controlling Intercellular Communications, Inha University School of Medicine, Incheon, Republic of Korea., Koh GY; Center for Vascular Research, Institute for Basic Science, Daejeon, Republic of Korea.

المصدر: Science (New York, N.Y.) [Science] 2024 Feb 09; Vol. 383 (6683), pp. 588-589. Date of Electronic Publication: 2024 Feb 08.

نوع المنشور: Journal Article

بيانات الدورية: Publisher: American Association for the Advancement of Science Country of Publication: United States NLM ID: 0404511 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9203 (Electronic) Linking ISSN: 00368075 NLM ISO Abbreviation: Science Subsets: MEDLINE

مواضيع طبية MeSH: Erectile Dysfunction*/therapy , Excitatory Amino Acid Transporter 1* , Fibroblasts*/metabolism , Fibroblasts*/physiology , Penile Erection*, Humans ; Male ; Mice ; Animals

مستخلص: Perivascular fibroblasts may underlie erectile dysfunction.

المؤلفون: Guimaraes EL; Department of Cell and Molecular Biology, Karolinska Institutet, 171 77 Stockholm, Sweden., Dias DO; Department of Cell and Molecular Biology, Karolinska Institutet, 171 77 Stockholm, Sweden., Hau WF; Department of Cell and Molecular Biology, Karolinska Institutet, 171 77 Stockholm, Sweden., Julien A; Department of Cell and Molecular Biology, Karolinska Institutet, 171 77 Stockholm, Sweden., Holl D; Department of Cell and Molecular Biology, Karolinska Institutet, 171 77 Stockholm, Sweden., Garcia-Collado M; Department of Medical Biochemistry and Biophysics, Division of Vascular Biology, Karolinska Institutet, 171 77 Stockholm, Sweden., Savant S; Department of Cell and Molecular Biology, Karolinska Institutet, 171 77 Stockholm, Sweden., Vågesjö E; Department of Medical Cell Biology, Division of Integrative Physiology, Uppsala University, 751 23 Uppsala, Sweden., Phillipson M; Department of Medical Cell Biology, Division of Integrative Physiology, Uppsala University, 751 23 Uppsala, Sweden., Jakobsson L; Department of Medical Biochemistry and Biophysics, Division of Vascular Biology, Karolinska Institutet, 171 77 Stockholm, Sweden., Göritz C; Department of Cell and Molecular Biology, Karolinska Institutet, 171 77 Stockholm, Sweden.

المصدر: Science (New York, N.Y.) [Science] 2024 Feb 09; Vol. 383 (6683), pp. eade8064. Date of Electronic Publication: 2024 Feb 09.

نوع المنشور: Journal Article

بيانات الدورية: Publisher: American Association for the Advancement of Science Country of Publication: United States NLM ID: 0404511 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9203 (Electronic) Linking ISSN: 00368075 NLM ISO Abbreviation: Science Subsets: MEDLINE

مواضيع طبية MeSH: Excitatory Amino Acid Transporter 1*/metabolism , Fibroblasts*/metabolism , Fibroblasts*/physiology , Penile Erection*/physiology , Penis*/blood supply , Penis*/physiology , Receptors, Notch*/metabolism, Animals ; Male ; Mice ; Blood Circulation ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Mice, Transgenic ; Signal Transduction ; Vasoconstriction ; Vasodilation

مستخلص: Penile erection is mediated by the corpora cavernosa, a trabecular-like vascular bed that enlarges upon vasodilation, but its regulation is not completely understood. Here, we show that perivascular fibroblasts in the corpora cavernosa support vasodilation by reducing norepinephrine availability. The effect on penile blood flow depends on the number of fibroblasts, which is regulated by erectile activity. Erection dynamically alters the positional arrangement of fibroblasts, temporarily down-regulating Notch signaling. Inhibition of Notch increases fibroblast numbers and consequently raises penile blood flow. Continuous Notch activation lowers fibroblast numbers and reduces penile blood perfusion. Recurrent erections stimulate fibroblast proliferation and limit vasoconstriction, whereas aging reduces the number of fibroblasts and lowers penile blood flow. Our findings reveal adaptive, erectile activity-dependent modulation of penile blood flow by fibroblasts.

المؤلفون: Liu G; Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.; Institute of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.; Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical University, Hefei, Anhui, China., Zhang Y; Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.; Institute of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.; Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical University, Hefei, Anhui, China., Zhang W; Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.; Institute of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.; Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical University, Hefei, Anhui, China., Wu X; Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.; Institute of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.; Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical University, Hefei, Anhui, China., Jiang H; Department of Urology, Peking University First Hospital Institute of Urology, Peking University Andrology Center, Beijing, China., Zhang X; Department of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.; Institute of Urology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China.; Anhui Province Key Laboratory of Genitourinary Diseases, Anhui Medical University, Hefei, Anhui, China.

المصدر: Andrology [Andrology] 2024 Feb; Vol. 12 (2), pp. 365-373. Date of Electronic Publication: 2023 Jun 19.

نوع المنشور: Journal Article

بيانات الدورية: Publisher: Wiley-Blackwell Country of Publication: England NLM ID: 101585129 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2047-2927 (Electronic) Linking ISSN: 20472919 NLM ISO Abbreviation: Andrology Subsets: MEDLINE

مواضيع طبية MeSH: Penile Erection* , Erectile Dysfunction*/diagnosis, Adult ; Humans ; Male ; Sleep, REM ; Sleep ; Health Status

مستخلص: Background: Sleep, particularly rapid eye movement sleep, has been found to be associated with sleep-related erections. While RigiScan is currently a more accurate method for monitoring nocturnal erectile events, the Fitbit, a smart wearable device, shows great potential for sleep monitoring.
Objectives: To analyze the relationship between sleep-related erections and sleep by recruiting sexually active, healthy men for simultaneous monitoring of sleep and nocturnal penile tumescence and rigidity.
Patients and Methods: Using Fitbit Charge2 and RigiScan, we simultaneously monitored nocturnal sleep and erections in 43 healthy male volunteers, and analyzed the relationship between sleep periods and erectile events with the Statistical Package for Social Sciences.
Results: Among all erectile events, 89.8% were related to rapid eye movement, and 79.2% of all rapid eye movement periods were associated with erectile events. Moreover, a statistical correlation was shown between the duration of rapid eye movement and the time of total erectile events (first night: 𝜌 = 0.316, p = 0.039; second night: 𝜌 = 0.370, p = 0.015).
Discussion and Conclusion: Our study shows a potential link between sleep-related erections and rapid eye movement sleep, which has implications for the current examination of sleep-related erections and further research into the mechanisms of erectile function. Meanwhile, the wearable device Fitbit has shown a potential promise for sleep monitoring in patients with erectile dysfunction. The results provide an alternative approach for further research on the relationship between erectile function and sleep with large sample sizes in the future.
(© 2023 American Society of Andrology and European Academy of Andrology.)

المؤلفون: Akhigbe RE; Department of Physiology, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria.; Reproductive Biology and Toxicology Research Laboratory, Oasis of Grace Hospital, Osogbo, Osun State, Nigeria., Hamed MA; Reproductive Biology and Toxicology Research Laboratory, Oasis of Grace Hospital, Osogbo, Osun State, Nigeria.; Department of Medical Laboratory Science, Afe Babalola University, Ado-Ekiti, Ekiti State, Nigeria.; The Brainwill Laboratories and Biomedical Services, Osogbo, Osun State, Nigeria., Odetayo AF; Reproductive Biology and Toxicology Research Laboratory, Oasis of Grace Hospital, Osogbo, Osun State, Nigeria.; Department of Physiology, University of Ilorin, Ilorin, Kwara State, Nigeria., Akhigbe TM; Reproductive Biology and Toxicology Research Laboratory, Oasis of Grace Hospital, Osogbo, Osun State, Nigeria.; Department of Agronomy, Osun State University (Ejigbo Campus), Osogbo, Nigeria., Oyedokun PA; Department of Physiology, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria.

المصدر: The aging male : the official journal of the International Society for the Study of the Aging Male [Aging Male] 2023 Dec; Vol. 26 (1), pp. 2205517.

نوع المنشور: Journal Article

بيانات الدورية: Publisher: Taylor & Francis Country of Publication: England NLM ID: 9808210 Publication Model: Print Cited Medium: Internet ISSN: 1473-0790 (Electronic) Linking ISSN: 13685538 NLM ISO Abbreviation: Aging Male Subsets: MEDLINE

مواضيع طبية MeSH: Penile Erection*/physiology , Erectile Dysfunction*/chemically induced , Erectile Dysfunction*/drug therapy, Humans ; Male ; Rats ; Animals ; Acetylcholinesterase/therapeutic use ; Up-Regulation ; Antiretroviral Therapy, Highly Active/adverse effects ; Zinc/therapeutic use ; Rats, Wistar ; Oxidation-Reduction

مستخلص: Purpose: HAART has been shown to impair sexual function and penile erection via perturbation of penile redox balance, while zinc has been established to exert antioxidant activity. Therefore, this study focused on the role and associated molecular mechanism of zinc in HAART-induced sexual and erectile dysfunction.
Materials and Methods: Twenty male Wistar rats were randomly grouped into four (n = 5 rats per group); the control, zinc-treated, HAART-treated, and HAART + zinc-treated groups. Treatments were per os daily for eight weeks.
Results: Zinc co-administration significantly improved HAART-induced increase in the latencies of mount, intromission, and ejaculations. Zinc also attenuated HAART-induced reduction in the motivation to mate, penile reflex/erection, and frequencies of mount, intromission, and ejaculations. In addition, zinc co-treatment improved HAART-induced decline in penile NO and cGMP, dopamine, and serum testosterone. More so, zinc prevented HAART-induced rise in penile activities of monoamine oxidase, acetylcholinesterase, phosphodiesterase-5, and arginase. Furthermore, concomitant treatment with zinc ameliorated HAART-induced penile oxidative stress and inflammation.
Conclusion: In conclusion, our present findings show that zinc improves sexual and erectile function in HAART-treated rats by upregulating erectogenic enzymes via the maintenance of penile redox balance.

المؤلفون: Shen Z; Department of Urology, University of Pittsburgh, Pittsburgh, PA, USA., Wang J; Department of Urology, University of Pittsburgh, Pittsburgh, PA, USA., Shen B; Department of Urology, University of Pittsburgh, Pittsburgh, PA, USA., Jian J; Department of Urology, University of Pittsburgh, Pittsburgh, PA, USA., Goosby K; Department of Urology, University of Pittsburgh, Pittsburgh, PA, USA., Wang W; Department of Urology, University of Pittsburgh, Pittsburgh, PA, USA., Beckel J; Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, PA, USA., de Groat WC; Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, PA, USA., Chermansky C; Department of Urology, University of Pittsburgh, Pittsburgh, PA, USA., Tai C; Department of Urology, University of Pittsburgh, Pittsburgh, PA, USA; Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, PA, USA; Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA. Electronic address: cftai@pitt.edu.

المصدر: Neuromodulation : journal of the International Neuromodulation Society [Neuromodulation] 2023 Dec; Vol. 26 (8), pp. 1817-1822. Date of Electronic Publication: 2022 Aug 06.

نوع المنشور: Journal Article

بيانات الدورية: Publisher: Elsevier Country of Publication: United States NLM ID: 9804159 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1525-1403 (Electronic) Linking ISSN: 10947159 NLM ISO Abbreviation: Neuromodulation Subsets: MEDLINE

مواضيع طبية MeSH: Penile Erection*/physiology , Spinal Cord Injuries*, Male ; Cats ; Animals ; Spinal Nerve Roots/physiology ; Electric Stimulation

مستخلص: Objective: This study aimed at determining whether stimulation of sacral spinal roots can induce penile erection in cats.
Materials and Methods: In anesthetized cats, a 20-gauge catheter was inserted into the corpus cavernosum to measure the penile pressure. Stimulus pulses (5-80 Hz, 0.2 ms) were applied through bipolar hook electrodes to sacral ventral roots alone or to combined ventral and dorsal roots of a single S1-S3 segment to induce penile pressure increases and penile erection.
Results: Stimulation of the S1 or S2 ventral root at 30 to 40 Hz induced observable penile erection with rigidity and the largest increase (169 ± 11 cmH 2 O) in penile pressure. Continuous stimulation (10 minutes) of afferent and efferent axons by simultaneous stimulation of the S1 or S2 dorsal and ventral roots at 30 Hz also produced a large increase (190 ± 8 cmH 2 O) in penile pressure that was sustainable during the entire stimulation period. After a complete spinal cord transection at the T9-T10 level, simultaneous stimulation of the S1 or S2 dorsal and ventral roots induced large (186 ± 9 cmH 2 O) and sustainable increases in penile pressure.
Conclusion: This study indicates the possibility to develop a novel neuromodulation device to restore penile erection after spinal cord injury using a minimally invasive surgical approach to insert a lead electrode through the sacral foramen to stimulate a sacral spinal root.
(Copyright © 2022 International Neuromodulation Society. Published by Elsevier Inc. All rights reserved.)