المؤلفون: Qi Wang, Hao Li, Ji-chang Qin, Lei Gao, Meixia Liu, Jiangang Liu, Feixue Wang, Yun Wei

المصدر: Chinese Journal of Integrative Medicine. 23:672-680

مصطلحات موضوعية: 0301 basic medicine, medicine.medical_specialty, Traditional medicine, biology, business.industry, Morris water navigation task, Radioimmunoassay, Decoction, General Medicine, Neuroprotection, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, Endocrinology, Bcl-2-associated X protein, Complementary and alternative medicine, Apoptosis, Internal medicine, medicine, biology.protein, Hippocampus (mythology), Pharmacology (medical), Tumor necrosis factor alpha, business, 030217 neurology & neurosurgery

الوصف: Huannao Yicong Decoction (还脑益聪方, HYD), an effective herbal formula against Alzheimer's disease (AD), has been proven to have neuroprotective action in amyloid β-protein1-42 (Aβ1-42)-induced rat model. This study was designed to characterize mechanisms by which HYD leads to suppression of inflflammation and apoptosis in the brains of Aβ1-42-induced rat. A total of 72 rats were divided into 6 groups, which were referred to as: sham operation group, model group, donepezil-treated group, HYD low-dose group (HYDL), HYD middle-dose group (HYDM) and HYD high-dose group (HYDH). Rats in HYDL, HYDM and HYDH were injected with Aβ1-42 at the CA1 region of hippocampus to form AD model and were fed the HYD extract at different dose of 3.78, 7.56 and 18.90 g crude drug/kg. The behavioral changes of rats were evaluated by Morris water maze (MWM) before sacrififice. Pathological changes of the brain tissue were evaluated using hematoxylin eosin (HE) staining. The levels of interleukin-1 (IL-1) and tumor necrosis factor-α (TNF-α) were measured by radioimmunoassay. The levels of Aβ and proteins that are associated with apoptosis such as B-cell lymphoma-2 protein (Bcl-2), Bcl-2-associated X protein (Bax), cysteine-aspartic protease (caspase)-3, -8, -9 and -12 in serum were measured by immunohistochemistry. Compared with the sham operation group, the spatial learning and memory abilities of AD rats were signifificantly decreased (P

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_________::04c885a0338820caff947dfdbfb54441
https://doi.org/10.1007/s11655-016-2255-1

المؤلفون: Xiao-ling Liu, Peng Wang, Jin Wang, Ling Zhang, Hong Wang, Yu-juan He, Xiao He, You-liang Wang

المصدر: Chinese Journal of Integrative Medicine. 22:362-369

مصطلحات موضوعية: Male, Carcinoma, Hepatocellular, Antineoplastic Agents, Apoptosis, 01 natural sciences, High-performance liquid chromatography, 03 medical and health sciences, 0302 clinical medicine, Bcl-2-associated X protein, In vivo, Animals, Medicine, Pharmacology (medical), fas Receptor, IC50, Cell Proliferation, bcl-2-Associated X Protein, Mice, Inbred BALB C, biology, Traditional medicine, Plant Extracts, business.industry, Cell growth, Liver Neoplasms, 010401 analytical chemistry, General Medicine, Reference Standards, Immunohistochemistry, Molecular biology, In vitro, Mitochondria, 0104 chemical sciences, Complementary and alternative medicine, 030220 oncology & carcinogenesis, Cancer cell, biology.protein, Female, Melia, business, Neoplasm Transplantation, Drugs, Chinese Herbal

الوصف: To investigate the anti-cancer effects of crude extract from Melia toosendan Sieb. et Zucc and its possible molecular mechanisms in vitro and in vivo. Transonic alcohol-chloroform extraction method was used to extract toosendanin from the bark of Melia toosendan Sieb. et Zucc, and the content of toosendanin in the crude extract was measured by high performance liquid chromatography (HPLC). Anti-cancer effects of crude extract from Melia toosendan Sieb. et Zucc were investigated in in vivo and in vitro studies. In the in vitro experiment, human hepatocellular carcinoma cell lines SMMC-7721 and Hep3B were co-incubated with toosendanin crude extract of different concentrations, respectively. In the in vivo experiment, BALB/c mice were subcutaneously inoculated with mouse hepatocellular carcinoma H22 cells and treated with crude extract. HPLC revealed the content of toosendanin was about 15%. Crude extract from Melia toosendan Sieb. et Zucc inhibited cancer cells growth in a dose- and time-dependent manner. The 50% inhibitory concentration (IC50, 72 h) was 0.6 mg/L for SMMC-7721 cells and 0.8 mg/L for Hep3B cells. Both high-dose [0.69 mg/(kg d)] and low-dose [0.138 mg/(kg d)] crude extract could markedly suppress cancer growth, and the inhibition rate was greater than 50%. Hematoxylin and eosin staining showed necrotic area in cancers and transmission electron microscopy displayed necrotic and apoptotic cancer cells with apoptotic bodies. Immunohistochemistry showed that the expression of Bax and Fas increased and the expression of Bcl-2 reduced. Toosendanin extract has potent anti-cancer effects via suppressing proliferation and inducing apoptosis of cancer cells in vivo and in vitro. The mechanism of apoptosis involves in mitochondrial pathway and death receptor pathway.

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::39947672b6957008da10da0b013cbe1e
https://doi.org/10.1007/s11655-015-2084-7

المؤلفون: Xin-mei Huang, Jie Zhang, Dijiong Wu, Zhe-wei Fei, Honghao Bi, Xi-ping Zhang

المصدر: Chinese Journal of Integrative Medicine. 22:674-684

مصطلحات موضوعية: Male, Pathology, medicine.medical_specialty, Apoptosis, Kidney, Protective Agents, Blood Urea Nitrogen, Injections, Rats, Sprague-Dawley, 03 medical and health sciences, 0302 clinical medicine, medicine, Animals, Pharmacology (medical), Radix, Aspartate Aminotransferases, Lung, bcl-2-Associated X Protein, Plant Extracts, business.industry, NF-kappa B, Alanine Transaminase, Bilirubin, gamma-Glutamyltransferase, General Medicine, Astragalus propinquus, Intercellular Adhesion Molecule-1, Immunohistochemistry, Toll-Like Receptor 4, Jaundice, Obstructive, Liver, Complementary and alternative medicine, Organ Specificity, Creatinine, 030220 oncology & carcinogenesis, Obstructive jaundice, business, 030217 neurology & neurosurgery, Drugs, Chinese Herbal

الوصف: To investigate the protective effects and mechanisms of Radix Astragali Injection on multiple organs of rats with obstructive jaundice (OJ).A total of 180 rats were randomly divided into the sham-operated, model control and treated groups (60 in each group). On 7, 14, 21 and 28 days after operation, the serum contents of alanine aminotransferase (ALT), aspartate aminotransferase (AST), r-glutamyl transpeptidase (r-GT), total bilirubin (TBil), direct bilirubin (DBil), blood urine nitrogen (BUN), and creatinine (CREA) were determined. And the pathological changes of livers, kidneys and lungs, and protein expressions of toll-like receptor-4 (TLR-4) of livers, intercellular adhesion molecule-1 (ICAM-1) of lungs, Bax and nuclear factor-kappa B (NF-κB), as well as apoptotic indexes of multiple organs were observed, respectively.The pathological severity scores of multiple organs (including livers on 7, 14, 21 and 28 days, kidneys on 14 and 28 days, and lungs on 14 days), serum contents of ALT (14 and 21 days), AST (14 days), TBil (7, 14, 21 and 28 days), DBil (14 and 21 days), BUN (28 days), protein expressions of TLR-4 (in livers, 28 days), Bax (in livers and kidneys, 21 days), and apoptotic indexes in livers (7 and 21 days) in the treated group were significantly lower than those in the model control group (P0.05 or P0.01).Radix Astragali Injection exerts protective effects on multiple organs of OJ rats by improving the pathological changes of lung, liver and kidney, decreasing the serum index of hepatic and renal function as well as inhibiting the protein expression of TLR-4 and Bax in the livers and Bax in the kidneys.

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::921862a6621cc4a632a43497f4956e6f
https://doi.org/10.1007/s11655-015-2048-y

المؤلفون: Xin-mei Hao, Lina Ma, Shao-chun Li, Ping He, Yikui Li, Jin-Yan Zhang, Lianda Li

المصدر: Chinese journal of integrative medicine. 23(8)

مصطلحات موضوعية: Cardiac function curve, Male, medicine.medical_specialty, Myocardial Infarction, Down-Regulation, Apoptosis, 030204 cardiovascular system & hematology, Pharmacology, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Internal medicine, Lactate dehydrogenase, medicine, In Situ Nick-End Labeling, Animals, Pharmacology (medical), Myocardial infarction, Disulfides, Systole, Rats, Wistar, Creatine Kinase, bcl-2-Associated X Protein, Ejection fraction, Allicin, biology, L-Lactate Dehydrogenase, business.industry, Myocardium, General Medicine, medicine.disease, Sulfinic Acids, Disease Models, Animal, Complementary and alternative medicine, chemistry, 030220 oncology & carcinogenesis, Heart Function Tests, Cardiology, biology.protein, Creatine kinase, Diltiazem hydrochloride, business

الوصف: To study the effects of allicin on cardiac function and underlying mechanism in rat model of myocardial infarction (MI).Ninety-four Wistar rats were randomly assigned to 6 groups (n=14-16 per group): sham control group [underwent thoracotomy without left anterior descending (LAD) occlusion and only received an injection of the same amount of citrate buffer], MI control group (subjected to LAD occlusion and only received an injection of same amount of citrate buffer), positive control group (subjected to LAD occlusion and received an injection of diltiazem hydrochloride at the dose of 1.5 mg/kg), and MI + allicin groups (subjected to LAD occlusion and received an injection of allicin at the doses of 1.2, 1.8, and 3.6 mg/kg). All of the drugs were administered intraperitoneally daily for 21 days. The infarct area was measured by myocardial staining. Hematoxylin-eosin staining was used to observe the pathological changes. Cardiac function parameters were assessed by echocardiography. The myocardial apoptotic index was estimated by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling staining. The expression of Bax and Bcl-2 were detected by quantificational real-time polymerase chain reaction and Western blot.Treatment with allicin could attenuate the myocardial infarct area (P0.05) and relieve the changes of the myocardium. The left ventricular anterior wall diastolic and systolic thicknesses were increased in the allicin-treated groups (P0.05), while there was no signifificant difference in the left ventricular posterior wall diastolic and systolic thickness (P0.05). The left ventricular internal diameter in systole, ejection fraction, fractional shortening, and stroke volume were dramatically elevated in allicin-treated rats (P0.05). Allicin dose-dependently reduced creatine kinase and lactate dehydrogenase levels (P0.05). The myocardial apoptotic index was also markedly lowered, and Bax expression was signifificantly decreased, whereas Bcl-2 expression exhibited an opposite trend in allicin-treated rats (P0.05).Allicin appears to exert a cardioprotective effect that may be linked to blocking Bcl-2/Bax signaling pathway-denpendent apoptosis, further improving cardiac function.

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f2c777c79eaee224e53ad29f23a534ea
https://pubmed.ncbi.nlm.nih.gov/27412589