المؤلفون: Mohammed AE; Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, 11671, Saudi Arabia., Alghamdi SS; Department of Pharmaceutical Sciences, College of Pharmacy, King Saud Bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia.; King Abdullah International Medical Research Center (KAIMRC), Riyadh, Saudi Arabia., Shami A; Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, 11671, Saudi Arabia., Suliman RS; Department of Pharmacy, Fatima College of Health Sciences, Abu Dhabi, 3798, United Arab Emirates., Aabed K; Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, 11671, Saudi Arabia., Alotaibi MO; Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, 11671, Saudi Arabia., Rahman I; Department of Basic Dental Sciences, College of Dentistry, Princess Nourah bint Abdulrahman University, Riyadh, 11671, Saudi Arabia.

المصدر: International journal of nanomedicine [Int J Nanomedicine] 2023 Apr 26; Vol. 18, pp. 2141-2162. Date of Electronic Publication: 2023 Apr 26 (Print Publication: 2023).

نوع المنشور: Journal Article

بيانات الدورية: Publisher: DOVE Medical Press Country of Publication: New Zealand NLM ID: 101263847 Publication Model: eCollection Cited Medium: Internet ISSN: 1178-2013 (Electronic) Linking ISSN: 11769114 NLM ISO Abbreviation: Int J Nanomedicine Subsets: MEDLINE

مواضيع طبية MeSH: Metal Nanoparticles* , Methicillin-Resistant Staphylococcus aureus* , Colonic Neoplasms*, Animals ; Precision Medicine ; Silver/pharmacology ; Escherichia coli ; Ampicillin ; Anti-Bacterial Agents/pharmacology ; Bacteria ; Plant Extracts/pharmacology ; Microbial Sensitivity Tests

مستخلص: Introduction: Biogenic silver nanoparticles (AgNPs) may be a feasible therapeutic option in the research and development towards selectively targeting specific cancers and microbial infections, lending a role in precision medicine. In-silico methods are a viable strategy to aid in drug discovery by identifying lead plant bioactive molecules for further wet lab and animal experiments.
Methods: Green synthesis of M-AgNPs was performed using the aqueous extract from the Malvaviscus arboreus leaves, characterized using UV spectroscopy, FTIR, TEM, DLS, and EDS. In addition, Ampicillin conjugated M-AgNPs were also synthesized. The cytotoxic potential of the M-AgNPs was evaluated using the MTT assay on MDA-MB 231, MCF10A, and HCT116 cancer cell lines. The antimicrobial effects were determined using the agar well diffusion assay on methicillin-resistant S. aureus (MRSA) and S. mutans, E. coli , and Klebsiella pneumoniae . Additionally, LC-MS was used to identify the phytometabolites, and in silico techniques were applied to determine the pharmacodynamic and pharmacokinetic profiles of the identified metabolites.
Results: Spherical M-AgNPs were successfully biosynthesized with a mean diameter of 21.8 nm and were active on all tested bacteria. Conjugation with ampicillin increased the susceptibility of the bacteria. These antibacterial effects were most predominant in Staphylococcus aureus (p < 0.0001). M-AgNPs had potent cytotoxic activity against the colon cancer cell line (IC 50 =29.5 μg/mL). In addition, four secondary metabolites were identified, Astragalin, 4-hydroxyphenyl acetic acid, Caffeic acid, and Vernolic acid. In silico studies identified Astragalin as the most active antibacterial and anti-cancer metabolite, binding strongly to the carbonic anhydrase IX enzyme with a comparatively higher number of residual interactions.
Discussion: Synthesis of green AgNPs presents a new opportunity in the field of precision medicine, the concept centered on the biochemical properties and biological effects of the functional groups present in the plant metabolites used for reduction and capping. M-AgNPs may be useful in treating colon carcinoma and MRSA infections. Astragalin appears to be the optimal and safe lead for further anti-cancer and anti-microbial drug development.
Competing Interests: The authors declare no conflicts of interest in this work.
(© 2023 Mohammed et al.)

المؤلفون: Hegazi RA; Department of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA., Saad RS, Mady H, Matarese LE, O'Keefe S, Kandil HM

المصدر: Nutrition (Burbank, Los Angeles County, Calif.) [Nutrition] 2006 Mar; Vol. 22 (3), pp. 275-82.

نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.

بيانات الدورية: Publisher: Elsevier Science Country of Publication: United States NLM ID: 8802712 Publication Model: Print Cited Medium: Print ISSN: 0899-9007 (Print) Linking ISSN: 08999007 NLM ISO Abbreviation: Nutrition Subsets: MEDLINE

مواضيع طبية MeSH: Plant Oils*, Colitis/*metabolism , Colonic Neoplasms/*metabolism , Cyclooxygenase 2/*metabolism , Dietary Fats, Unsaturated/*administration & dosage , Fish Oils/*adverse effects, Animals ; Colitis/epidemiology ; Colitis/pathology ; Colonic Neoplasms/epidemiology ; Colonic Neoplasms/pathology ; Corn Oil ; Immunohistochemistry ; Interleukin-10/deficiency ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Olive Oil ; Random Allocation ; Severity of Illness Index

مستخلص: Objective: The effects of different dietary oils on the development of colitis-associated colon cancer have not been studied. The present study examined the effect of different dietary oils on the severity of chronic colitis, development of colitis-associated premalignant changes, and colonic expression of cyclooxygenase-2 (COX-2) in interleukin-10 knockout (IL-10-/-) mice.
Methods: IL-10-/- mice were fed chow supplemented with corn oil (CO; control, n=28), olive oil (OO; n=29), or fish oil (FO; n=35) for 12 wk and their colons were studied for colitis score, premalignant changes, and COX-2 expression.
Results: The average colitis score was higher in the FO than in the CO group. Similarly, the incidence of severe colitis (score>or=3) was significantly higher in the FO than in the CO and OO groups (50% versus 7.7% and 3.7%, respectively, P<0.05). Dysplasia was more frequent in the FO and less frequent in the OO than in the CO group (47% and 4% versus 15%, respectively, P<0.05). Conversely, aberrant crypt foci and crypt index were significantly higher in the FO than in the CO group. Colitis score, aberrant crypt foci, and crypt index did not differ between the OO and CO groups. COX-2 immunostaining was significantly lower in the OO than in CO group (P<0.05) but not different between the FO and CO groups.
Conclusions: In IL-10-/- mice, fish oil exacerbates chronic colitis and colitis-associated premalignant changes. Conversely, olive oil inhibits COX-2 immunostaining and decreases the risk of neoplasia associated with chronic colitis.