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1دورية أكاديمية
المؤلفون: Maria V. O. Santos, Andréia M. Silva, Leonardo V. C. Aquino, Lhara R. M. Oliveira, Samara S. J. Moreira, Moacir F. Oliveira, Alexandre R. Silva, Alexsandra F. Pereira
المصدر: Animals, Vol 13, Iss 12, p 1955 (2023)
مصطلحات موضوعية: wild ungulates, density gradient centrifugation, swim-up, washing by centrifugation, Veterinary medicine, SF600-1100, Zoology, QL1-991
الوصف: Methods for seminal plasma (SP) removal and the selection of collared peccary sperm for fertilization were compared. The experiments evaluated the following: the (I) impact of centrifugation for SP removal before swim-up for sperm selection and (II) a comparison of different Percoll® gradient densities (PG 45–90% and PG 35–70%). Non-selected sperm served as the control. Sperm quality was assessed based on motility patterns, morphology, membrane functional integrity, viability, reactive oxygen species (ROS), glutathione (GSH), and DNA integrity. Subsequently, the most successful group in the previous experiment and washing by centrifugation (WC) were compared for motility patterns and fertilization using pig oocytes. Swim-up decreased motility and enhanced ROS compared to the control. Centrifugation before swim-up harmed integrity and viability compared to the control. PG 45–90% (96.8 vs. 69.7 vs. 40.7 µm/s) allowed for a better velocity average pathway (VAP), a better velocity straight line, and better linearity (LIN) than those of the control and PG 35–70% (88.4 vs. 56.0 vs. 27.3 µm/s). Thus, PG 45–90% was used for fertilization. PG 45–90% obtained a higher VAP, a higher amplitude of the lateral head, straightness, and higher LIN than those of the control and WC. Cleavage (25.2–26.3%) and morula (8.1–10.5%) rates did not differ between the groups. Therefore, PG 45–90% and WC were efficient in isolating collared peccary sperm capable of fertilizing pig oocytes.
وصف الملف: electronic resource
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2دورية أكاديمية
المؤلفون: Luana G. P. Bezerra, Andréia M. Silva, Artur P. Jurema, Maiko R. T. Dantas, Ana G. Pereira, Moacir F. Oliveira, Pierre Comizzoli, Alexandre R. Silva
المصدر: Animals, Vol 13, Iss 9, p 1483 (2023)
مصطلحات موضوعية: bird reproduction, ratites, sperm morphology, epididymis, vas deferens, Veterinary medicine, SF600-1100, Zoology, QL1-991
الوصف: The objective was to characterize morphological, morphometric, and ultrastructural changes in rhea spermatozoa between the epididymis and the vas deferens. Sperm samples were collected from the reproductive tracts of seven adult individuals and evaluated for sperm characteristics using brightfield microscopy as well as ultrastructural features using scanning electron microscopy (SM). Mean sperm count tended to increase in the vas deferens (378.0 ± 135.0 × 106) compared to the epididymis (201.0 ± 77.4 × 106). Percentages of motile sperm grew from 37.0 ± 4.9% in the epididymis to 58.5 ± 7.7% in the vas deferens. The proportion of normal spermatozoa was 75.6 ± 1.8% and most common defects were bent tails (9.7 ± 0.9%). However, these proportions were not different between epididymis and vas deferens. SM analysis revealed further features of rhea spermatozoa. Normal rhea spermatozoa were threadlike with an acrosome (0.95 ± 0.0 µm), head (7.53 ± 0.01 µm), midpiece (2.08 ± 0.01 µm), and tail (30.7 ± 0.06 µm). Lengths of sperm acrosome, head, midpiece, and tail were longer in the vas deferens compared to the epididymis. Our findings suggest that rhea spermatozoa undergo a maturation process during the passage from the epididymis to the vas deferens.
وصف الملف: electronic resource
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3دورية أكاديمية
المؤلفون: Jael S. Batista, Tiago S. Teófilo, Francisco H.A. Silva, Natanael S. Félix, Emerson C.O. Silva, Hélio N. Araújo Júnior, Radan E.M. Oliveira, Moacir F. Oliveira
المصدر: Pesquisa Veterinária Brasileira, Vol 42 (2022)
مصطلحات موضوعية: Digestive system disorders, agoutis, Dasyprocta leporina, captivity, semiarid, Brazil, wild animals, necropsy, Veterinary medicine, SF600-1100
الوصف: ABSTRACT: The objective of this study was to describe the clinical and pathological aspects of diseases of the digestive system in agoutis (Dasyprocta leporina Linnaeus, 1758) diagnosed by the “Laboratório de Patologia Veterinária” (Veterinary Pathology Laboratory) of the “Universidade Federal Rural do Semi-Árido” (UFERSA), from January 2018 to February 2020. During the study period, necropsy and a survey of the clinical history of 27 agoutis were performed, 25.93% (7/27) of which were diagnosed with digestive system diseases. The percentages of digestive tract diseases among the diagnosed were: acute carbohydrate overload (11.12%), gastric ulcer (7.41%), gastric volvulus (3.70%), and intestinal volvulus (3.70%). Studies on the occurrence rate of these diseases, as well as the description of their clinical and anatomopathological aspects, may serve as a basis for guiding the appropriate management in the breeding of these animals.
وصف الملف: electronic resource
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4دورية أكاديمية
المؤلفون: Alexsandra F. Pereira, Leonardo V.C Aquino, Matheus B. Nascimento, Ferdinando V.F. Bezerra, Alana A. Borges, Érika A. Praxedes, Moacir F. Oliveira
المصدر: Pesquisa Veterinária Brasileira, Vol 41 (2021)
مصطلحات موضوعية: Wild rodents, Dasyprocta leporina, Galea spixii, ultrastructure, morphology, morphometry, ear skin, cartilage, cryobanks, wildlife animals, Veterinary medicine, SF600-1100
الوصف: ABSTRACT: Skin and cartilage have been the main source for the recovery of somatic cells to be used in conservation strategies in wild mammals. In this sense, an important step for the cryopreservation of these samples is to recognize the properties of the skin and cartilage. Thus, knowing that the skin may differ among species and aiming to contribute to the establishment of cryobanks, the study examined the differences in the ear skin and cartilage of wild rodents from South America, agouti (Dasyprocta leporina) and spix’s yellow-toothed cavy (Galea spixii). Ultrastructural and quantitative methods were used to measure skin and cartilage thickness, density of collagen and elastic fibers, cell type number and distribution, and proliferative activity. Although ultrastructural analysis revealed a similar pattern between species, morphometric analysis of the skin and cartilage showed differences between agoutis and cavies regarding thickness of epidermis layers (corneum: 5.3±2.5μm vs. 3.9±0.6μm; intermediate: 16.4±6.2μm vs. 23.4±8.1μm; basal: 9.9±2.1μm vs. 4.8±0.5μm), dermis (183.1±44.0μm vs. 258.2±22.9μm), total skin (211.8±46.0μm vs. 290.3±23.7μm) and perichondrium (27.6±6.1μm vs. 10.5±1.8μm). A greater number of epidermal cells (61.7±15.2 vs. 24.8±7.6) and chondrocytes (32.7±9.0 vs. 27.5±4.7) were observed in agouti, while the cavy presented a greater number of melanocytes (12.6±4.7 vs. 29.9±6.2), keratinocytes (14.7±4.2 vs. 29.8±7.6), and fibroblasts (103.6±24.7 vs. 112.2±11.3). Moreover, a higher percentage of collagen fibers and proliferative activity was observed in the skin of cavies, when compared to the skin of agoutis. Therefore, there are differences between agouti and cavy for ear skin and cartilage, requiring the establishment of species-specific cryopreservation protocols.
وصف الملف: electronic resource
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5دورية أكاديمية
المؤلفون: Andréia M. Silva, Ana G. Pereira, Luana G. P. Bezerra, Samara S. Jerônimo Moreira, Alexsandra F. Pereira, Moacir F. Oliveira, Pierre Comizzoli, Alexandre R. Silva
المصدر: Animals, Vol 12, Iss 6, p 738 (2022)
مصطلحات موضوعية: biobanking, testicular tissue, wild rodents, vitrification, permeating cryoprotectants, Veterinary medicine, SF600-1100, Zoology, QL1-991
الوصف: This study measured the effects of different freezing techniques and permeating cryoprotectants on the preservation of testicular tissues from adult red-rumped agoutis. Tissue biopsies (3.0 mm3) from five individuals were allocated to different experimental groups: control (non-cryopreserved); slow freezing (SF), solid-surface vitrification (SSV), and conventional vitrification (CV). Each method used dimethyl sulfoxide (DMSO), ethylene glycol (EG), or a DMSO + EG combination. Morphology, viability, mitochondrial activity, and proliferative potential were assessed in fresh and frozen tissue samples. Testicular morphology was better using SSV with a combination of DMSO and EG. Across the different cryopreservation approaches, as well as cryoprotectant combinations, cell viability was comparable. Regarding mitochondrial activity, DMSO + EG/SSV or CV, and DMSO + EG/CV were similar to the EG/SF group, which was the best group that provided values similar to fresh control groups. Adequate preservation of the proliferative potential of spermatogonia, Leydig cells, and Sertoli cells was obtained using SSV with DMSO + EG. Overall, the use of SSV with DMSO + EG was the best protocol for the preservation of testicular tissues from adult red-rumped agoutis.
وصف الملف: electronic resource
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6دورية أكاديمية
المؤلفون: Dardo N. Ferreiro, Sergio A. Conde-Ocazionez, João H.N. Patriota, Luã C. Souza, Moacir F. Oliveira, Fred Wolf, Kerstin E. Schmidt
المصدر: iScience, Vol 24, Iss 1, Pp 101882- (2021)
مصطلحات موضوعية: Biological Sciences, Neuroscience, Developmental Neuroscience, Cellular Neuroscience, Sensory Neuroscience, Science
الوصف: Summary: All rodents investigated so far possess orientation-selective neurons in the primary visual cortex (V1) but – in contrast to carnivores and primates – no evidence of periodic maps with pinwheel-like structures. Theoretical studies debating whether phylogeny or universal principles determine development of pinwheels point to V1 size as a critical constraint. Thus, we set out to study maps of agouti, a big diurnal rodent with a V1 size comparable to cats'. In electrophysiology, we detected interspersed orientation and direction-selective neurons with a bias for horizontal contours, corroborated by homogeneous activation in optical imaging. Compatible with spatial clustering at short distance, nearby neurons tended to exhibit similar orientation preference. Our results argue against V1 size as a key parameter in determining the presence of periodic orientation maps. They are consistent with a phylogenetic influence on the map layout and development, potentially reflecting distinct retinal traits or interspecies differences in cortical circuitry.
وصف الملف: electronic resource
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7دورية أكاديمية
المؤلفون: Carlos M. Oliveira Júnior, Ferdinando V.F. Bezerra, Felipe V. Câmara, André M. do Vale, Gleidson B. de Oliveira, Alexandre R. da Silva, Carlos E. Ambrosio, Moacir F. Oliveira
المصدر: Pesquisa Veterinária Brasileira, Vol 36, Iss 3, Pp 227-236 (2016)
مصطلحات موضوعية: Glândulas salivares, roedor, cutia, Dasyprocta aguti, macroscopia e microscopia, Veterinary medicine, SF600-1100
الوصف: Resumo: Estudos acerca da morfologia de animais silvestres servem de subsídio para trabalhos de manejo e preservação de diferentes espécies, pois fornecem informações para a tomada de medidas que auxiliem na manutenção destes em cativeiro, na preservação em habitat natural ou mesmo para ações voltadas a reintrodução ao habitat de origem. Estudos referentes à morfologia de cutias abordam os diversos sistemas, mas nenhum faz referência à arquitetura ou estrutura de suas glândulas salivares. Assim este trabalho objetivou descrever macro e microscopicamente as glândulas salivares maiores de cutias. Foram utilizados dez animais adultos para o desenvolvimento de metodologias relativas à macroscopia propriamente dita das glândulas, microscopia de luz, microscopia eletrônica de transmissão e microscopia eletrônica de varredura. Foram identificadas quatro glândulas salivares maiores nos animais estudados, denominadas parótida, mandibular, zigomática e sublingual. As glândulas apresentaram-se como sendo do tipo tubuloacinares e contendo em seu parênquima ductos dos mais variados tamanhos. Com exceção da glândula parótida, que era estritamente serosa, as demais eram mistas. Da mesma forma, apenas a glândula mandibular foi identificada a presença de ducto do tipo granuloso. Apresentando as cutias os quatro pares de glândulas salivares maiores, estes animais podem servir de modelo para os estudos acerca das mudanças anatômicas sofridas pelos roedores para se adaptar aos diversos habitat do planeta.
وصف الملف: electronic resource
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8دورية أكاديمية
المؤلفون: Radan E.M. Oliveira, Gleidson B. Oliveira, Procássia M.L. Barbosa, Ferdinando V.F. Bezerra, José Fernando Gomes de Albuquerque, Carlos E. Ambrósio, Maria A. Miglino, Moacir F. Oliveira
المصدر: Pesquisa Veterinária Brasileira, Vol 35, Iss 8, Pp 762-766 (2015)
مصطلحات موضوعية: Artérias, arco aórtico, tronco braquiocefálico, preá, Galea spixii, Caviidae, roedor., Veterinary medicine, SF600-1100
الوصف: Resumo: O preá é um roedor típico da caatinga pertencente à família Caviidae. Considerando a inexistência de dados sobre o arco aórtico do preá, foi realizado este estudo tendo como objetivo descrever os ramos colaterais do arco aórtico neste cavídeo, e dessa forma, contribuir com dados para biologia da espécie. Foram utilizados vinte preás machos provenientes de estudos anteriores e encontravam-se armazenados em freezer no Centro de Multiplicação de Animais Silvestres (CEMAS/UFERSA). Os animais foram descongelados, a cavidade torácica foi aberta, a aorta canulada e o sistema vascular lavado com solução salina e em seguida, injetado látex Neoprene corado com pigmento vermelho, amarelo ou branco. Posteriormente, os animais foram fixados em formol e depois de 72 horas, dissecados e analisados, sendo obtidos desenhos esquemáticos e os exemplares mais representativos fotografados. O arco aórtico do preá emitiu como ramos colaterais, o tronco braquiocefálico e a artéria subclávia esquerda. O tronco braquiocefálico originou na maioria das peças estudadas, a artéria carótida comum esquerda e o tronco braquiocarotídeo, do qual surgem as artérias subclávia direita e carótida comum direita. As artérias subclávias direita e esquerda em todos os animais estudados emitiram a artéria vertebral, a artéria torácica interna, a artéria cervical superficial, o tronco costocervical e a artéria axilar. O padrão da formação do arco aórtico do preá assemelhou-se ao observado em outros roedores, tais como no mocó, no porquinho-da-índia e na chinchila.
وصف الملف: electronic resource
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9دورية أكاديمية
المؤلفون: Lívia B. Campos, Gislayne C.X. Peixoto, Gabriela L. Lima, Thibério S. Castelo, Ana L.P. Souza, Moacir F. Oliveira, Alexandre R. Silva
المصدر: Pesquisa Veterinária Brasileira, Vol 35, Iss 2, Pp 188-192 (2015)
مصطلحات موضوعية: Dasyprocta leporina, roedores, colpocitologia, ecografia ovariana, Veterinary medicine, SF600-1100
الوصف: O objetivo deste estudo foi monitorar o ciclo estral em cutias (Dasyprocta leporina) criadas em cativeiro no semiárido brasileiro. Durante 70 dias, cinco cutias foram diariamente submetidas a citologia esfoliativa vaginal, e o monitoramento ultrassonográfico ovariano foi realizado a cada três dias. Um total de 8 ciclos estrais foi completamente monitorado, com duração de 28,2±0,7 dias, variando de 24 a 31 dias. Pela citologia esfoliativa vaginal, houve uma predominância de células superficiais nas fases de proestro e estro (P0,05). Os folículos foram identificados durante as fases estrogênicas (proestro e estro), com diâmetro médio de 1±0,5mm. Em apenas 12,5% das fases luteais, corpos lúteos medindo 1,4±0,9mm foram identificados. Conclui-se que a associação da citologia vaginal e da ultrassonografia ovariana constitui uma alternativa viável para o monitoramento de ciclos estrais e identificação das fases estrogênicas em cutias da espécie Dasyprocta leporina
وصف الملف: electronic resource
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10دورية أكاديمية
المؤلفون: Amilton C. Santos, Diego C. Viana, Bruno M. Bertassoli, Gleidson B. Oliveira, Daniela M. Oliveira, Ferdinando V.F. Bezerra, Moacir F. Oliveira, Antônio C. Assis-Neto
المصدر: Pesquisa Veterinária Brasileira, Vol 35, Iss 1, Pp 89-94 (2015)
مصطلحات موضوعية: Galea spixii, fêmeas, preás, preservação, reprodução, roedores., Veterinary medicine, SF600-1100
الوصف: The Galea spixii inhabits semiarid vegetation of Caatinga in the Brazilian Northeast. They are bred in captivity for the development of researches on the biology of reproduction. Therefore, the aim of this study is characterize the estrous cycle of G. spixii, in order to provide information to a better knowledge of captive breeding of the species. The estrous cycle was monitored by vaginal exfoliative cytology in 12 adult females. After the detection of two complete cycles in each animal, the same were euthanized. Then, histological study of the vaginal epithelium, with three females in each phase of the estrous cycle was performed; five were paired with males for performing the control group for estrous cycle phases, and three other were used to monitor the formation and rupture of vaginal closure membrane. By vaginal exfoliative cytology, predominance of superficial cells in estrus, large intermediate cells in proestrus, intermediate and parabasal cells, with neutrophils, in diestrus and metestrus respectively was found. Estrus was detected by the presence of spermatozoa in the control group. By histology, greater proliferation of the vaginal epithelium in proestrus was observed. We conclude that the estrous cycle of G. spixii lasts 15.8 ± 1.4 days and that the vaginal closure membrane develops until complete occlusion of the vaginal ostium, breaking after few days. Future studies may reveal the importance of this fact for the reproductive success of this animal.
وصف الملف: electronic resource
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