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1دورية أكاديمية
المؤلفون: Manali Arora, Aditya Abhishek, Nitesh Singh, Vishal Thakker, Sheenam Azad, Aakash Gupta, Navdeep Singh Sidhu, Rajiv Azad
المصدر: Journal of Clinical and Diagnostic Research, Vol 18, Iss 03, Pp 01-04 (2024)
مصطلحات موضوعية: calcifications, enhancement, miettinen risk classification, necrosis, Medicine
الوصف: Introduction: Gastrointestinal Stromal Tumours (GISTs) are the most common mesenchymal tumours of gastrointestinal tract. A high postsurgical recurrence and metastatic rate have created a need for a presurgical risk profile identification system. Aim: To assess the association between morphological Computed Tomography (CT) parameters with the pathological risk profile and analyse which CT features can predict the risk grading of GISTs. Materials and Methods: This was a retrospective cohort study based on imaging and histopathological data of 26 patients with pathologically proven GISTs presenting to the Department of Radiodiagnosis of a tertiary hospital in the northern Indian Himalayan foothills over a period of five years from July 2018 to June 2023. CT imaging features including size, growth pattern, margins, enhancement, calcifications, necrosis, intralesional haemorrhage, enlarged feeding vessels, direct organ invasion, and associations such as ascites and lymphadenopathy were studied. All lesions were classified as per Miettinen risk classification into no risk, very low-risk, low, moderate, and high-risk lesions. Analysis was done by the Chi-square test. Predictive analysis was carried out by computing the odds ratio and performing regression analysis on significantly associated imaging features. Results: Out of 26 patients, the study group comprised 16 males (61.54%) and 10 females (38.46%). The most common decade of presentation was the 6th decade with the mean age of presentation being 55.81±4.23 years. Twelve patients were grouped under intermediate to high-risk grading. Lesion size >5 cm (p-value=0.0171, OR=19.12), ill-defined margins (p-value=0.0048, OR=18.33), intralesional necrosis (p-value=0.0053, OR=19.8), and enlarged feeding vessels (p-value=0.012, OR=21.27) were identified as imaging features with significant association and predictive ability for high-risk lesions. The strongest predictive ability for a high-risk profile was shown by ill-defined margins (R2=0.381) and intralesional necrosis (R2=0.3287). Conclusion: A preoperative Contrast Enhanced Computed Tomography (CECT) assessment provides a comprehensive imaging profile for GISTs as well as a fair accuracy of risk profile prediction via various singular and clustered morphological parameters.
وصف الملف: electronic resource
Relation: https://www.jcdr.net/articles/PDF/19175/68803_CE[Ra1]_F(IS)_QC_REF_PAT(KK_IS)_PF1(AG_KM)_PFA(AG_KM)_PN(KM).pdf; https://doaj.org/toc/2249-782X; https://doaj.org/toc/0973-709X
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2دورية أكاديمية
المؤلفون: Murtaza Bohra, Nitesh Singh, Sai Vittal Battula, Vidyadhar Singh, Anil Annadi, Evropi Toulkeridou, Panagiotis Grammatikopoulos
المصدر: Results in Physics, Vol 57, Iss , Pp 107382- (2024)
مصطلحات موضوعية: Magnetocaloric effect, Cation disorder, Curie temperature, ZnFe2O4 thin films, Physics, QC1-999
الوصف: The detection and characterization of the Magnetocaloric Effect (MCE) at the nanoscale and its subsequent impact on next-generation cooling on-chip devices are of paramount importance. Here, we demonstrate that the MCE properties housed within a ferrimagnetic phase arise in disordered ZnFe2O4. The robustness of the MCE in terms of magnetic entropy change ΔSmax (=0.07–0.09 J/kg-K at 0.5 T) emerges in ZnFe2O4 thin films at larger temperature ranges 425–710 K, reaching above room temperature. By fitting the magnetization vs. temperature curves with a Gaussian-type distribution, we observed a correlation between the broadening of the MCE peak, Curie temperature (TC) distribution, and grain size distributions in nanocrystalline ZnFe2O4 thin films. The width of TC distributions increases (up to 150 K) and consequently, the ΔS curves show an extended full width at half maximum, resulting in larger relative cooling power (RCP), and a wider change in specific heat capacities (ΔCP) and adiabatic temperatures (ΔTad). This study reveals that tuning the relative strengths of the sublattice spin configuration of antiferromagnetic (AFM) ZnFe2O4 through in-situ and ex-situ heat treatment paves the way for the alteration of MCE properties.
وصف الملف: electronic resource
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3دورية أكاديمية
المؤلفون: Manoj Gadewar, G.K. Prashanth, M. Ravindra Babu, M.S. Dileep, PA Prashanth, Srilatha Rao, M. Mahadevaswamy, Mithun Kumar Ghosh, Nitesh Singh, S.K. Mandotra, Avnish Chauhan, Sarvesh Rustagi, Rajesh Yogi, Sampath Chinnam, Baber Ali, Sezai Ercisli, Emine Orhan
المصدر: Journal of Saudi Chemical Society, Vol 28, Iss 1, Pp 101774- (2024)
مصطلحات موضوعية: ZnO nanostructures, Green/Alternate synthesis, Plant extracts, Microbes, Drug delivery, Antitubercular, Chemistry, QD1-999
الوصف: In recent years, zinc oxide nanoparticles (ZNPs) have gained tremendous attention attributed to their unique properties. It is the most desirable group of nanostructures as far as both structure and properties are concerned. It is an adaptable material that has distinctive properties, such as high sensitivity, large specific area, non-toxicity, good compatibility, and a high isoelectric point, which favors it to be considered with a few exceptions. They are utilized in an expanding number of mechanical items, for example, elastic, paint, coating, cosmetics, supercapacitors, Lithium batteries, color-sensitized sun-powered cells, photocatalysis, biosensors, biomedical and characteristic applications. They have brought a promising potential up in biomedicine, especially in the fields of anticancer and antibacterial fields, which are locked in with their solid ability to trigger plenitude reactive oxygen species (ROS) creation, discharge zinc particles, and instigate cell apoptosis. ZNPs have also been successfully utilized to transport and load drugs to target locations as drug carriers, reducing undesirable toxicity and non-target effects and increasing the synergistic effects. In addition, ZNPs display outstanding light assets and this property has made them one of the chief bioimaging applicants Thus, this paper reviews the biological synthesis of ZNPs by the microbes, the mechanisms of the biological synthesis, parameters for the optimization process and their potential application as an antimicrobial agent and feed supplement in the animal industry as well as their toxicological hazards on animals. Here, we summarize the synthesis and recent advances of ZNPs in the biomedical fields, which will be helpful for facilitating their future research progress and focusing on biomedical fields.
وصف الملف: electronic resource
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4دورية أكاديمية
المؤلفون: Chanchal Malik, Sudhanshu Dwivedi, Tilahun Rabuma, Ravinder Kumar, Nitesh Singh, Anil Kumar, Rajesh Yogi, Vinod Chhokar
المصدر: Frontiers in Genetics, Vol 14 (2023)
مصطلحات موضوعية: Asparagus racemosus, gene ontology, secondary metabolism, real time PCR, gene expression, Genetics, QH426-470
الوصف: Asparagus racemosus is known for its diverse content of secondary metabolites, i.e., saponins, alkaloids, and a wide range of flavonoids. Flavonoids, including phenols and polyphenols, have a significant role in plant physiology and are synthesized in several tissues. Despite the diverse role of flavonoids, genetic information is limited for flavonoid biosynthesis pathways in A. racemosus. The current study explores full-scale functional genomics information of A. racemosus by de novo transcriptome sequencing using Illumina paired-end sequencing technology to elucidate the genes involved in flavonoid biosynthesis pathways. The de novo assembly of high-quality paired-end reads resulted in ∼2.3 million high-quality reads with a pooled transcript of 45,647 comprising ∼76 Mb transcriptome with a mean length (bp) of 1,674 and N50 of 1,868bp. Furthermore, the coding sequence (CDS) prediction analysis from 45,647 pooled transcripts resulted in 45,444 CDS with a total length and mean length of 76,398,686 and 1,674, respectively. The Gene Ontology (GO) analysis resulted in a high number of CDSs assigned to 25,342 GO terms, which grouped the predicted CDS into three main domains, i.e., Biological Process (19,550), Molecular Function (19,873), and Cellular Component (14,577). The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database was used to categorize 6,353 CDS into 25 distinct biological pathway categories, in which the majority of mapped CDS were shown to be related to translation (645), followed by signal transduction (532), carbohydrate metabolism (524), folding, sorting, and degradation (522). Among these, only ∼64 and 14 CDSs were found to be involved in the phenylpropanoid and flavonoid biosynthesis pathways, respectively. Quantitative Real-time PCR was used to check the expression profile of fourteen potential flavonoid biosynthesis pathway genes. The qRT-PCR analysis result matches the transcriptome sequence data validating the Illumina sequence results. Moreover, a large number of genes associated with the flavonoids biosynthesis pathway were found to be upregulated under the induction of methyl jasmonate. The present-day study on transcriptome sequence data of A. racemosus can be utilized for characterizing genes involved in flavonoid biosynthesis pathways and for functional genomics analysis in A. racemosus using the reverse genetics approach (CRISPR/Cas9 technology).
وصف الملف: electronic resource
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5دورية أكاديمية
المؤلفون: Aayushi Khajuria, Nitesh Singh Malan, Rishabh Bajpai, Dimpy Kapoor, Ashish Mishra, Shivkumar S Harti, Medha Kulkarni, Deepak Joshi
المصدر: Annals of Indian Academy of Neurology, Vol 26, Iss 4, Pp 461-468 (2023)
مصطلحات موضوعية: bhramari pranayama, breathing pattern, cortical correlates, electroencephalography, humming bee sound, Neurology. Diseases of the nervous system, RC346-429
الوصف: Context: Previous research has shown the vast benefits associated with BhP. However, the dynamics of cortical activity in connection with Bhramari sound have not been investigated yet. Aim: To investigate the cortical activity in connection with Bhramari sound. Settings and Design: Humming sound was analyzed with a custom-made nasal device consisting of MAX4466 sensor time synchronized with the EEG setup. We anticipated that the modulation of cortical activity with the humming sound (either of long or short durations) leaves its effects after the Pranayama, which helps to understand the positive impacts of BhP. Methods and Material: 30 participants were instructed to perform the BhP for a period of 90 seconds. We proposed to investigate the cortical correlates before, during, and after the BhP through EEG. A custom-made nasal device consisting of MAX4466 sensor time synchronized with the EEG setup was used for analyzing the humming sound. Statistical Analysis Used: A paired t-test (P < 0.05) with a Bonferroni correction is carried out to explore the statistically significant difference in power spectral density (PSD) values. Results: Results show that the relative spectral power in theta band for short humming durations (less than or equal to 9 seconds) was similar on the frontal cortex during and after the Pranayama practice (P > 0.05) in most of the subjects. Conclusions: In conclusion, for the immediate positive effects of BhP, the humming duration should be kept less than or equal to 9 seconds. A wearable sound recording system can be developed in the future as a feedback system that provides biofeedback to the user so that a constant humming duration can be maintained.
وصف الملف: electronic resource
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6دورية أكاديمية
المؤلفون: Bikash Kumar Rajak, Priyanka Rani, Pranabesh Mandal, Rajender Singh Chhokar, Nitesh Singh, Durg Vijay Singh
المصدر: Frontiers in Agronomy, Vol 5 (2023)
مصطلحات موضوعية: acetyl-CoA carboxylase (ACCase), carboxyl transferase (CT) domain, mutation, herbicide resistance, rational drug discovery, safeners, Agriculture, Plant culture, SB1-1110
الوصف: Acetyl-CoA carboxylase (ACCase: EC 6.4.1.2) is one of the essential biotins containing enzymes required by plants for fatty acid synthesis and elongation. The unique enzyme is present in its homomeric form in all the Gramineae family, making it a suitable target for developing herbicides selectively against weeds of the Gramineae family. One such example is infestation of Phalaris minor in winter wheat crop fields, where aryloxyphenoxypropionates (FOP); cyclohexanediones (DIM) and phenyl pyrazoline (DEN) group of ACCase inhibiting herbicides are used. However, the increasing number of ACCase herbicide resistant weed populations has compelled agro-scientists to seek varied possibilities for weed control, through Integrated Weed Management (IWM) strategies. Developing new potential herbicides to regain sensitivity in weeds could be an approach to weed control. The current advancement in computational techniques could be of aid in developing new herbicide-like molecules by exploring the genomics, proteomics and structural details of catalytic sites of herbicide action in crops as well as weeds.
وصف الملف: electronic resource
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7دورية أكاديمية
المؤلفون: Ravi Prakash Srivastava, Sachin Kumar, Lav Singh, Mayank Madhukar, Nitesh Singh, Gauri Saxena, Shivaraman Pandey, Arpit Singh, Hari Prasad Devkota, Praveen C. Verma, Shatrughan Shiva, Sumira Malik, Sarvesh Rustagi
المصدر: Frontiers in Nutrition, Vol 10 (2023)
مصطلحات موضوعية: antimicrobial, antioxidant, HPLC, MTT assay, pharmacology, Selinum carvifolia, Nutrition. Foods and food supply, TX341-641
الوصف: Antibiotic resistance poses a serious threat to public health, raising the number of diseases in the community. Recent research has shown that plant-derived phenolic compounds have strong antimicrobial, antifungal, and cytotoxic properties against a variety of microorganisms and work as great antioxidants in such treatments. The goal of the current work is to evaluate the anticancerous, antibacterial, antifungal, antioxidant, and cytotoxicity activities in the extracts of the different plant parts (leaves, stems, and roots) of S. carvifolia (L.) L. This is a medicinally important plant and has been used for different kinds of diseases and ailments such as hysteria and seizures. The phenolic compounds from the different plant parts were analyzed using HPLC and the following were found to be present: chlorogenic acid, gallic acid, rutin, syringic acid, vanillic acid, cinnamic acid, caffeic acid, and protocatechuic acid. Gallic acid was found to have the highest concentration (13.93 mg/g), while chlorogenic acid (0.25 mg/g) had the lowest. The maximum TPC value, which ranged from 33.79 to 57.95 mg GAE/g dry extract weight, was found in the stem. Root extract with 9.4 mg RE/g had the greatest TFC level. In the leaf and stem extracts, the RSC ranged from 0.747 mg/mL to 0.734 mg/1 mL GE/g dry extract weight, respectively. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay was used to measure in vitro antioxidant activity. In a concentration-dependent way, promising antioxidant activity was reported. Moreover, 3,5-dinitrosalicylic acid (DNSA) and the Folin–Ciocalteu phenol reagent technique were used to determine reducing sugar content and total phenolic content, respectively. Antibacterial activity against eight strains (MIC: 250–1,000 μg/mL) was analyzed, and the stem extract exhibited maximum activity. Antifungal activity was also assessed, and potent activity was reported especially in the extract obtained from the stem. Cytotoxicity was evaluated using an MTT assay in the A549 cell line, where different doses (0.0625, 0.125, 0.25, 0.5, and 1 mg/mL) of leaf, root, and stem extracts were used. Treatment with these extracts reduced the cell viability, indicating that S. carvifolia may possess anticancer potential, which can be of great therapeutic value.
وصف الملف: electronic resource
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8دورية أكاديمية
المؤلفون: Atul K. Upadhyay, Shekhar Mallick, Ranjan Singh, Lav Singh, Nitesh Singh, S. K. Mandotra, Arpit Singh, Ravi Prakash Srivastava, Shivaraman Pandey, Gauri Saxena
المصدر: Frontiers in Microbiology, Vol 14 (2023)
مصطلحات موضوعية: volatilization, algae, arsenic, biotransformation, Nannochloropsis sp., A. doliolum, Microbiology, QR1-502
الوصف: A novel laboratory model was designed to study the arsenic (As) biotransformation potential of the microalgae Chlorella vulgaris and Nannochloropsis sp. and the cyanobacterium Anabaena doliolum. The Algae were treated under different concentrations of As(III) to check their growth, toxicity optimization, and volatilization potential. The results revealed that the alga Nannochloropsis sp. was better adopted in term of growth rate and biomass than C. vulgaris and A. doliolum. Algae grown under an As(III) environment can tolerate up to 200 μM As(III) with moderate toxicity impact. Further, the present study revealed the biotransformation capacity of the algae A. doliolum, Nannochloropsis sp., and Chlorella vulgaris. The microalga Nannochloropsis sp. volatilized a large maximum amount of As (4,393 ng), followed by C. vulgaris (4382.75 ng) and A. doliolum (2687.21 ng) after 21 days. The present study showed that As(III) stressed algae-conferred resistance and provided tolerance through high production of glutathione content and As-GSH chemistry inside cells. Thus, the biotransformation potential of algae may contribute to As reduction, biogeochemistry, and detoxification at a large scale.
وصف الملف: electronic resource
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9دورية أكاديمية
المؤلفون: Mukesh Kumar, Ravinder Mehra, Rajesh Yogi, Nitesh Singh, Raj Kumar Salar, Gauri Saxena, Sarvesh Rustagi
المصدر: Frontiers in Sustainable Food Systems, Vol 7 (2023)
مصطلحات موضوعية: Detannification, fruit juice, Klebsiella pneumoniae, tannic acid, tea cream solubilization, Nutrition. Foods and food supply, TX341-641, Food processing and manufacture, TP368-456
الوصف: The presence of high tannin content in fruit juices and other beverages generates bitterness and haze formation with a concomitant deterioration of their quality. This problem could be circumvented by treating these food products with the enzyme tannase (tannin acyl hydrolase EC 3.1.1.20), which contains the ability to hydrolyze the ester and depside linkages present in hydrolyzable tannins. In the present study, a low molecular weight (38.20 kDa) tannase purified from Klebsiella pneumoniae KP715242 was used for clarification and detannification of orange juice, beer, and tea cream solubilization. The initial tannin content of orange juice was reduced to 42.45% upon treatment with 4.5 U/mL of purified tannase at 35°C for 3.5 h. Beer haze was clarified by incubating the colloidal suspension with the purified enzyme. Under the optimum condition of temperature (30°C), incubation time (3.5 h), and enzyme concentration (4.5 U/mL), the initial tannin content of beer was reduced to 46.47%. Tea cream solubilization was achieved by treating the tea extract with varying enzyme concentrations at different time intervals and temperatures. The maximum reduction in the tea tannin content (55%) was observed at 35°C when the enzyme was incubated for 3 h at a concentration of 6.0 U/mL. The results of this investigation could be further exploited at an industrial scale in the detannification of fruit juice, beer, and tea.
وصف الملف: electronic resource
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10دورية أكاديمية
المصدر: Scientific Reports, Vol 12, Iss 1, Pp 1-12 (2022)
الوصف: Abstract Friction stir welding is a novel technique for joining ferrous and non-ferrous materials in a solid state. The groove fill techniques are most popular and generally used by researchers to dope reinforcement in the FSWed zone to improve the properties of joints. The main drawback of this technique is that a few amounts of reinforcement material come out from the groove during the fabrication of the joint. In the present work, the adhesive-assisted reinforcement technique was used to overcome this problem for the fabrication of particulates reinforced friction stirred weld joint. In the present work, the aluminum alloy plate edges were coated with a thin layer of TiB2. The coated and non-coated edge plates were joined using friction stir welding at the rotational speed of 1400 and 2240 rpm, and welding speed of 32 mm/min using a taper threaded pin tool. The tensile strength of coated edge plate welded joints was found highest in comparison to non-coated joints which was 39.74% superior. The percentage elongation of coated edge joint was observed about 1.5 times lower than the non-coated edge plate joint. The flexure strength of TiB2 reinforced coated edge joint was found about 1.5 times higher. However, the impact strength of coated edge plate was found nearly three times lower than the uncoated edge joints. The TiB2 coated edge joints reveal 22.75% higher hardness than the non-coated edge plate joints welded at the rotational speed of 2240.
وصف الملف: electronic resource
Relation: https://doaj.org/toc/2045-2322
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