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المؤلفون: Alan M. Lambowitz, Rick Russell, Jennifer L. Stamos, Jun Yao, Alfred M. Lentzsch

المصدر: The Journal of Biological Chemistry

مصطلحات موضوعية: Models, Molecular, DNA, Complementary, RNA virus, Retroelements, high-throughput RNA-Seq, Stereochemistry, RdRP, RNA-dependent RNA polymerase, RNA-dependent RNA polymerase, Crystallography, X-Ray, Biochemistry, Geobacillus stearothermophilus, transcriptomics, cDNA, complementary DNA, chemistry.chemical_compound, Adapter (genetics), TGIRT, thermostable group II intron reverse transcriptase, PDB, Protein Data Bank, Complementary DNA, thermostable group II intron reverse transcriptase, NTA, nontemplated nucleotide addition, CV, column volume, Molecular Biology, Polymerase, X-ray crystallography, biology, RNA, RNA-Directed DNA Polymerase, Templates, Genetic, Cell Biology, Group II intron, Introns, PE, primer extension, Long terminal repeat, Reverse transcriptase, Cell biology, retrovirus, RNA, Bacterial, chemistry, TS, template switching, NTE, N-terminal extension, RT, reverse transcriptase, Nucleic acid, biology.protein, LTR, long terminal repeat, Primer (molecular biology), DNA, Research Article

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b458fcf4b0b09c19a9203a6de206a868
https://doi.org/10.1101/2021.05.13.443781

المؤلفون: Matthew Drew, Vanessa Wall, Kaitlyn Sadtler, Troy Taylor, Dominic Esposito, William K. Gillette, John-Paul Denson, Kelly Snead, Simon Messing, Jennifer Mehalko

المصدر: Protein Expression and Purification
bioRxiv
article-version (status) pre
article-version (number) 1

مصطلحات موضوعية: 0106 biological sciences, Signal peptide, TFF, tangential flow filtration, Population, Enzyme-Linked Immunosorbent Assay, Antibodies, Viral, AnSEC, analytical size exclusion chromatography, 01 natural sciences, Article, Serology, 03 medical and health sciences, chemistry.chemical_compound, RBD, receptor binding domain, Antigen, Protein Domains, 010608 biotechnology, Protein biosynthesis, Protein production, Humans, IMAC, immobilized metal ion affinity chromatography, education, CV, column volume, 030304 developmental biology, 0303 health sciences, education.field_of_study, Chemistry, SARS-CoV-2, SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, COVID-19, Promoter, ELISA, enzyme-linked immunosorbent assay, MWCO, molecular weight cut-off, SBP, streptavidin-binding peptide, Receptor-binding domain, Biochemistry, Cell culture, Spike Glycoprotein, Coronavirus, ELISA, SEC, size exclusion chromatography, DNA, Protein Binding, Biotechnology

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1cc806c227ce12e4cdbb0964da63baf2

المؤلفون: Jelena Rajkovic, Juan A. Hermoso, José Berenguer, Elisa Beneventi, Mercedes Sánchez-Costa, Aurelio Hidalgo, Jacobo Cruces, María-Isabel González-Siso, Ana Torrado, Ivanna Rivera, Vega Miguel-Ruano, María L. Rúa, Kamila Knapik, Manuel Becerra, José M. Otero

المساهمون: ALBA Synchrotron, European Commission, Ministerio de Ciencia, Innovación y Universidades (España), UAM. Departamento de Biología Molecular

المصدر: Investigo. Repositorio Institucional de la Universidade de Vigo
Universidade de Vigo (UVigo)
Digital.CSIC. Repositorio Institucional del CSIC
instname
Biblos-e Archivo. Repositorio Institucional de la UAM
RUC. Repositorio da Universidade da Coruña
Computational and Structural Biotechnology Journal, Vol 19, Iss, Pp 1214-1232 (2021)
Computational and Structural Biotechnology Journal
RUC: Repositorio da Universidade da Coruña
Universidade da Coruña (UDC)

مصطلحات موضوعية: DMSO, dimethyl sulfoxide, Biochemistry, Esterase, 0302 clinical medicine, Structural Biology, Enzime-Substrate Complex, CHCA, cyclohexane carboxylic acid, CV, column volume, chemistry.chemical_classification, 0303 health sciences, biology, Chemistry, NP, naproxen, Biología y Biomedicina / Biología, 2306 Química Orgánica, pNP, 4-nitrophenol, 3. Good health, Computer Science Applications, DSF, Differential scanning fluorimetry, 030220 oncology & carcinogenesis, Crystal structures, Enzyme-substrate complex, Crystal Structure, 2301 Química Analítica, HSL, hormone-sensitive lipase, Research Article, Biotechnology, CMC, critical micellar concentration, Hydrolase Fold, Stereochemistry, Biophysics, FLU, fluorescein, 03 medical and health sciences, Metagenomic, PPL, Porcine Pancreatic Lipase, Genetics, Lipase, ComputingMethodologies_COMPUTERGRAPHICS, 030304 developmental biology, Enzyme substrate complex, 2403 Bioquímica, Thermophilic esterase, Thermophile, Substrate (chemistry), Active site, LDAO, N,N-dimethyldodecylamine N-oxide, Enzyme, biology.protein, α/β hydrolase fold, MNP, methyl-naproxen, Linker, TP248.13-248.65

وصف الملف: application/pdf

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b5cb848bc105fe00f6966a667d7b1477
https://linkinghub.elsevier.com/retrieve/pii/S2001037021000519

المؤلفون: Kornelia M. Mikula, Robert Kolodziejczyk, Adrian Goldman

المساهمون: Molecular and Integrative Biosciences Research Programme, Biochemistry and Biotechnology

المصدر: Journal of Structural Biology

مصطلحات موضوعية: MECHANISM, UspA1, Crystallography, X-Ray, Protein Structure, Secondary, Moraxella catarrhalis, DOMAIN, Structural Biology, PROGRAM, CEACAM1, carcinoembryonic antigen-related cell adhesion molecule 1, Usps, ubiquitous surface proteins, Trimeric autotransporter adhesin, CRYSTAL-STRUCTURE, LB, Luria broth, NETWORK, A1, CV, column volume, 0303 health sciences, biology, p, polar, C4b-binding protein, Chemistry, h, hydrophobic, 030302 biochemistry & molecular biology, C3d protein, SAXS, small-angle X-ray scattering, EPITHELIAL-CELLS, 3. Good health, Biochemistry, Complement C3d, Chromatography, Gel, Bacterial Outer Membrane Proteins, Protein Binding, TAA, trimeric autotransporter adhesin, FIBRONECTIN, Virulence, Article, 03 medical and health sciences, PBS, phosphate buffered saline, TRIMERIC AUTOTRANSPORTER ADHESINS, Binding site, complement system, 030304 developmental biology, ComputingMethodologies_COMPUTERGRAPHICS, Binding Sites, RECEPTOR, Microscale thermophoresis, biology.organism_classification, Complement system, TAAs, Protein Fragment, 1182 Biochemistry, cell and molecular biology, Anisotropy, X-ray structure, IPTG, isopropyl β-d-1-thiogalactopyranoside

وصف الملف: application/pdf

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::19c4afb445d2ff62bb9a6b7e580c96c0

المؤلفون: Swainsbury, David J.K., Proctor, Matthew S., Hitchcock, Andrew, Cartron, Michaël L., Qian, Pu, Martin, Elizabeth C., Jackson, Philip J., Madsen, Jeppe, Armes, Steven P., Hunter, C. Neil

المصدر: Biochimica et Biophysica Acta

مصطلحات موضوعية: Models, Molecular, CL, cardiolipin, Rba. sphaeroides, Light-Harvesting Protein Complexes, Rhodobacter sphaeroides, PG, phosphatidylglycerol, PE, phosphatidylethanolamine, Thylakoids, Article, cytc2, cytochrome c2, PC, phosphatidylcholine, Electron Transport Complex III, Membrane Lipids, Synechocystis, Synechocystis sp. PCC 6803, Bacterial Proteins, Microscopy, Electron, Transmission, Mw, Mass average molecular weight, cytb6f, cytochrome b6f complex, SMA, PSII, photosystem II, Rba. sphaeroides, Rhodobacter sphaeroides, TEM, transmission electron microscopy, CV, column volume, UQ10, ubiquinone 10, RC-LH1-PufX, reaction centre light harvesting complex 1-PufX core, SMA, poly(styrene-alt-maleic acid), SMALPs, styrene-maleic acid lipid particles, TLC, thin layer chromatography, cytbc1, cytochrome bc1 complex, Cytochrome bc1, LH2, light harvesting 2 complex, Cytochrome b6f, Synechocystis, Maleates, Photosystem II Protein Complex, Bacterial Chromatophores, WT, wild-type, AFM, atomic force microscopy, PMF, proton motive force, Cytochrome b6f Complex, Energy Transfer, Solubility, HPLC, high performance liquid chromatography, PSI, photosystem I, β-DDM, n-Dodecyl-beta-Maltoside, Polystyrenes, SQDG, sulfoquinovosyl diacylglycerol, Quinone pool

URL الوصول: https://explore.openaire.eu/search/publication?articleId=pmid________::cde361addacf7a4a6a5f9d778f2bfb6b
http://europepmc.org/articles/PMC5805856

المؤلفون: Renate Schreiber, Raymond J. Owens, Monika Oberer, Roland Viertlmayr, Natalia Kulminskaya, Christoph Heier, Mariana Colaço-Gaspar, Rudolf Zechner, Peter Hofer, Robert Zimmermann, Claudia Radler

المصدر: The Journal of Biological Chemistry

مصطلحات موضوعية: Acylation, Gene Expression, Hormone-sensitive lipase, PNPLA, patatin-like phospholipase, Biochemistry, His6, hexahistidine tag, Mice, SMT3, small ubiquitin-like modifier, Transacylation, mATGL, mouse ATGL, Protein purification, Sf9 Cells, MAG, monoacylglycerol, CV, column volume, G0S2, NusA, transcription termination/antitermination protein, biology, Chemistry, Hydrolysis, ABHD5, Ni2+, nickel (II) ion, Recombinant Proteins, TRX, thioredoxin, MTBE, methyl tert-butyl ether, CGI-58, comparative gene identification-58, DGAT, diacylglycerol acyltransferase, NTA, nitrilotriacetic acid, HSL, hormone-sensitive lipase, Research Article, SEC, size-exclusion chromatography, LD, lipid droplet, TAG, triacylglycerol, MBP, maltose-binding protein, Spodoptera, TEV, tobacco etch virus, GST, glutathione-S-transferase, Animals, Humans, Lipase, Molecular Biology, PNPLA2, Diacylglycerol kinase, ATGL, adipose triglyceride lipase, CGI-58, OPPF, Oxford Protein Production Facility, FA, fatty acid, TCEP, Tris(2-carboxyethyl)phosphine, Cell Biology, HEK293T, human embryonic kidney 293T, Fusion protein, Monoacylglycerol lipase, ONC, overnight culture, HEK293 Cells, Adipose triglyceride lipase, lipolysis, biology.protein, BSA, bovine serum albumin, G0S2, G0/G1 switch gene 2, DAG, diacylglycerol

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6978f21be8369c3f6c69b81d2252b270
https://doi.org/10.1016/j.jbc.2021.101206

المؤلفون: Leroy Versteeg, Bin Zhan, Brian Keegan, Jungsoon Lee, Rakesh Adhikari, Wen-Hsiang Chen, Maria Elena Bottazzi, Joanne Altieri Rivera, Zhuyun Liu, Jeroen Pollet, Rakhi Tyagi Kundu, Maria Jose Villar, Portia M. Gillespie, Junfei Wei, Ulrich Strych, Peter J. Hotez, Cristina Poveda, Ana Carolina de Araujo Leao

المصدر: Biochimica et Biophysica Acta. General Subjects

مصطلحات موضوعية: Biophysical characterization, 0301 basic medicine, CoV, coronavirus, Gene Expression, medicine.disease_cause, Biochemistry, law.invention, 0302 clinical medicine, law, %CV, coefficient of variation, 030212 general & internal medicine, Cloning, Molecular, CV, column volume, CD, circular dichroism, Peptide sequence, Coronavirus, DLS, dynamic light scattering, Genetics, COVID-19, Coronavirus disease 2019, RBD, receptor-binding domain, FS, fermentation supernatant, Recombinant Proteins, h, hour, Spike Glycoprotein, Coronavirus, Recombinant DNA, Biotechnology, ACE-2, angiotensin-converting enzyme 2, S, spike, COVID-19 Vaccines, Protein domain, Biophysics, Biology, Article, 03 medical and health sciences, Protein Domains, medicine, SARS, severe acute respiratory syndrome, Amino Acid Sequence, DO, dissolved oxygen, Molecular Biology, SARS-CoV-2, Wild type, Yeast, Protein tertiary structure, Protein Structure, Tertiary, 030104 developmental biology, Saccharomycetales, P. pastoris, Function (biology)

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ffb7d4f5d7a58a35155871be2c4858f0
https://doi.org/10.1016/j.bbagen.2021.129893

المؤلفون: Martin Zoltner, William N. Hunter, Paul K. Fyfe, Tracy Palmer

المصدر: Biochemical Journal

مصطلحات موضوعية: Models, Molecular, Cytoplasm, Protein Conformation, BCG, Bacille Calmette–Guérin, MALDI–TOF-MS, matrix-assisted laser-desorption ionization–time-of-flight MS, Plasma protein binding, early secretory antigenic target of 6 kDa system 1 (ESX-1), Crystallography, X-Ray, Biochemistry, Protein structure, rmsd, root-mean-square deviation, ESX-1, ESAT-6 system 1, T7SS, Type VII secretion system, Threonine, CV, column volume, Peptide sequence, 0303 health sciences, protein kinase, Recombinant Proteins, Transport protein, Protein Transport, LB, Luria–Bertani, DDM, dodecyl maltoside, ess, ESX-1 secretion system, ESI–Q–TOF-MS, electrospray ionization–quadrupole–time-of-flight MS, PEG3350, poly(ethylene glycol) 3350, SAD, single-wavelength anomalous diffraction, Research Article, Protein Binding, SeMet, selenomethionine, Spectrometry, Mass, Electrospray Ionization, Staphylococcus aureus, Gram-positive bacterium, Molecular Sequence Data, pseudokinase, SPR, surface plasmon resonance, BAP, biotin-acceptor peptide, Biology, ESAT-6, early secreted antigenic target of 6 kDa, TEV, tobacco etch virus, 03 medical and health sciences, Bacterial Proteins, Two-Hybrid System Techniques, protein secretion, Escherichia coli, IPTG, isopropyl-β-D-thiogalactopyranoside, Secretion, Amino Acid Sequence, Molecular Biology, X-ray crystallography, 030304 developmental biology, BN-PAGE, Blue native PAGE, Sequence Homology, Amino Acid, 030306 microbiology, Membrane Proteins, Cell Biology, Surface Plasmon Resonance, DOPC, 1,2-dioleoyl-sn-glycero-3-phosphocholine, Protein Structure, Tertiary, Secretory protein, Membrane protein, DTT, dithiothreitol, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Protein Multimerization, MWCO, molecular-mass cut-off

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7bc85d082ea4397193aae60c84b993fa
https://doi.org/10.1042/bj20121209

المؤلفون: Kota Kano, Daisuke Sugimori, Yusaku Matsumoto

المصدر: FEBS Open Bio

مصطلحات موضوعية: SBL, lecithin from soybean, PI, l-α-phosphatidylinositol, Expression, pNPP, p-nitrophenyl palmitate, SaEst, esterase of Streptomyces albus J1074, chemistry.chemical_compound, POPA, 1-Palmitoyl-2-oleoyl-sn-glycerol-3-phosphate, SxPLA1, phospholipase A1 from Serratia sp. xjF1, Phospholipase A1, LPC, l-α-Lysophosphatidylcholine, CV, column volume, DMPA, 1,2-Dimyristoyl-sn-glycero-3-phosphate, Purification, SaPLA1, phospholipase A1 from Streptomyces albidoflavus, DLS, dynamic light scattering, chemistry.chemical_classification, biology, EGGL, lecithin from egg yolk, PG, l-α-phosphatidylglycerol, PC, l-α-phosphatidylcholine, Phosphatidic acid, pNPD, p-nitrophenyl decanoate, POPE, 1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine, Biochemistry, pNPS, p-nitrophenyl stearate, PLA1, phospholipase A1, Characterization, SMPLA1, phospholipase A1 from Serratia sp. MK1, Article, General Biochemistry, Genetics and Molecular Biology, pNPO, p-nitrophenyl octanoate, Streptomyces albidoflavus, Phospholipase A2, DOPE, 1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine, POPG, 1-Palmitoyl-2-oleoyl-sn-glycero-3-phospho-rac-(1-glycerol), FFA, free fatty acid, POPC, 1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, Enzyme kinetics, PLD, phospholipase D, TSB, tryptic soy broth, Phospholipase A, Chromatography, EcPLA1, phospholipase A1 from Escherichia coli, Phospholipase D, PLA2, phospholipase A2, PS, l-α-phosphatidyl-l-serine, Active site, pNPL, p-nitrophenyl laurate, SsEst, esterase from S. scabies, DPPC, 1,2-Dipalmitoyl-sn-glycero-3-phosphocholine, pNPB, p-nitrophenyl butyrate, Enzyme, chemistry, biology.protein

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3553662466fb41f8fc9a2ea2303be2a4
https://doi.org/10.1016/j.fob.2012.09.006