نتائج البحث - "Egill Richard"

المساهمون: Plasticité du Génome Bactérien - Bacterial Genome Plasticity (PGB), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Hub Bioinformatique et Biostatistique - Bioinformatics and Biostatistics HUB, Institut Pasteur [Paris] (IP)-Université Paris Cité (UPCité), Collège Doctoral, Sorbonne Université (SU), Microbiologie Fondamentale et Pathogénicité (MFP), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), Viral DNA Integration and Chromatin Dynamics Network (DyNAVir), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS)-Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Recherche en Informatique (LRI), CentraleSupélec-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Génomique évolutive des Microbes / Microbial Evolutionary Genomics, This work was supported by the Institut Pasteur, the Centre National de la Recherche Scientifique (CNRS-UMR 3525), the Fondation pour la Recherche Médicale (FRM Grant No. EQU202103012569), ANR Chromintevol (ANR-21-CE12-0002-01), and by the French Government’s Investissement d’Avenir program Laboratoire d’Excellence ‘Integrative Biology of Emerging Infectious Diseases’ [ANR-10-LABX-62-IBEID]., We would like to thank Gaspard Macaux for its experimental help. We also thank Jason Bland, a native English speaker, for helpful reading of the manuscript and all the lab members for helpful discussion., C.L and D.M designed the research. C.L, E.R, C.V, B.D, V.P, F.L and T.N performed the experiments. G.M and F. L performed the computational analysis of Deep sequencing data. J.C and E.P.C.R performed the bioinformatics genomics analysis. C.L and G. M wrote the draft of the manuscript. All authors read, amended the manuscript, and approved its final version., ANR-21-CE12-0002,Chromintevol,Etude de l'impact des intégrons chromosiques sur l'évolution des bactéries(2021), ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010)

مصطلحات موضوعية: Bacterial evolution, genome-wide mapping, antibiotic resistance, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, site-specific recombination, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, cassette dynamics, attC sites, Integron

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::58a0cdfc222f67e58bbadd860da648ec
https://doi.org/10.1101/2022.09.11.507474

5

Genetic drivers of chromosomal integron stability

المؤلفون: Egill Richard, Baptiste Darracq, Eloi Littner, Claire Vit, Clémence Whiteway, Julia Bos, Florian Fournes, Geneviève Garriss, Valentin Conte, Delphine Lapaillerie, Vincent Parissi, François Rousset, Ole Skovgaard, David Bikard, Eduardo P. C. Rocha, Didier Mazel, Céline Loot

المساهمون: Plasticité du Génome Bactérien - Bacterial Genome Plasticity (PGB), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Ecole Doctorale Complexité du Vivant (ED515), Sorbonne Université (SU), Génomique évolutive des Microbes / Microbial Evolutionary Genomics, Direction générale de l'Armement (DGA), Microbiologie Fondamentale et Pathogénicité (MFP), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), Viral DNA Integration and Chromatin Dynamics Network (DyNAVir), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS)-Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), Biologie de Synthèse - Synthetic biology, Université Paris Cité (UPCité)-Microbiologie Intégrative et Moléculaire (UMR6047), Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Department of Science, Systems and Models, Roskilde Universitet [Roskilde], This work was supported by the Institut Pasteur, the Centre National de la Recherche Scientifique (CNRS-UMR 3525), the Fondation pour la Recherche Médicale (FRM Grant No. EQU202103012569), the ANR Chromintevol (ANR-21-CE12-0002-01), the French Government’s Investissement d’Avenir program Laboratoire d’Excellence ‘Integrative Biology of Emerging Infectious Diseases’ [ANR-10-LABX-62-IBEID], the Ministère de l’Enseignement Supérieur et de la Recherche and the Direction Générale de l’Armement (DGA), We thank Jun Teramoto and Akihiko Kondo for sharing their construct for base-editing. We thank Marc Monot, Laurence Ma, Juliana Pipoli Da Fonseca and Thomas Cocklaer from the Biomics platform, C2RT, Institut Pasteur, Paris, France, ANR-21-CE12-0002,Chromintevol,Etude de l'impact des intégrons chromosiques sur l'évolution des bactéries(2021), ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010)

مصطلحات موضوعية: sitespecific recombination, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, mobile elements, genetic stability, bacterial evolution and plasticity, genome organization, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, toxin-antitoxin cassettes, sedentary chromosomal integron

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::92f096d46eab638a46a7778b380b54e0
https://doi.org/10.1101/2022.08.03.502626

6

Cassette recruitment in the chromosomal Integron ofVibrio cholerae

المؤلفون: Claire Vit, Xavier Eyer, Vincent Parissi, Florian Fournes, Egill Richard, Didier Mazel, Clemence Whiteway, Céline Loot, Delphine Lapaillerie

المساهمون: Plasticité du Génome Bactérien - Bacterial Genome Plasticity (PGB), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), École Doctorale Bio Sorbonne Paris Cité [Paris] (ED BioSPC), Université Sorbonne Paris Cité (USPC)-Université de Paris (UP), Institut de Cancérologie Strasbourg (ICANS), Vrije Universiteit Brussel (VUB), Hôpital Lariboisière-Fernand-Widal [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Microbiologie cellulaire et moléculaire et pathogénicité (MCMP), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), This work was supported by the French Government’s Investissement d’Avenir program Laboratoire d’Excellence ‘Integrative Biology of Emerging Infectious Diseases’ [ANR-10-LABX-62- IBEID] and ‘Fondation pour la Recherche Médicale’, ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), Faculty of Sciences and Bioengineering Sciences, Department of Bio-engineering Sciences, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), École Doctorale Bio Sorbonne Paris Cité [Paris] (ED562 - BioSPC), Université Sorbonne Paris Cité (USPC)-Université Paris Cité (UPCité), Microbiologie Fondamentale et Pathogénicité (MFP), Institut Pasteur, Centre National de la Recherche Scientifique, the French Government’s Investissement d’Avenir program Laboratoire d’Excellence Integrative Biology of Emerging Infectious Diseases [ANR-10-LABX-62- IBEID], Fondation pour la Recherche Médicale and Ecole Doctorale Complexité du Vivant. Funding for open access charge: Institut Pasteur, We would like to thank Evelyne Krin for providing the N16961 recA mutant and Sandra Arroyo-Beck and Marcos Manero for their experimental help. We also thank Jason Bland for helpful reading of the manuscript and all the lab members for helpful discussion., Author contributions: C.L., D.M. and V.P. designed the research. C.L., C.V., E.R., F.F., C.W., X.E. and D.L. performed the experiments. C.L. and C.V. wrote the draft of the manuscript. All authors read, amended the manuscript, and approved its final version.

المصدر: Nucleic Acids Research
Nucleic Acids Research, 2021, 49 (10), pp.5654-5670. ⟨10.1093/nar/gkab412⟩

مصطلحات موضوعية: MESH: Integrases, AcademicSubjects/SCI00010, [SDV]Life Sciences [q-bio], Genome Integrity, Repair and Replication, Integron, medicine.disease_cause, Integrons/genetics, Integrons, Plasmid, SOS response, Vibrio cholerae, Recombination, Genetic, Genetics, MESH: Gene Expression Regulation, Bacterial, 0303 health sciences, Rec A Recombinases/genetics, biology, MESH: Escherichia coli, [SDV.BID.EVO]Life Sciences [q-bio]/Biodiversity/Populations and Evolution [q-bio.PE], Recombination, Genetic/genetics, MESH: Integrons, Integrase, MESH: Rec A Recombinases, Adaptive selection, MESH: Recombination, Genetic, Gene Transfer, Horizontal, Integrases/genetics, Chromosomes, 03 medical and health sciences, Vibrio cholerae/genetics, Escherichia coli, medicine, 030304 developmental biology, Integrases, 030306 microbiology, Chromosomes/genetics, Gene Transfer, Horizontal/genetics, Gene Expression Regulation, Bacterial, Gene Expression Regulation, Bacterial/genetics, biochemical phenomena, metabolism, and nutrition, bacterial infections and mycoses, biology.organism_classification, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, MESH: Gene Transfer, Horizontal, Rec A Recombinases, Transformation (genetics), Escherichia coli/metabolism, biology.protein, bacteria, MESH: Chromosomes, MESH: Vibrio cholerae, Bacteria

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::20a01301c318589ff672975b1c1743b4
https://doi.org/10.1101/2020.11.24.395434

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