المؤلفون: Ueda, George, Antanasijevic, Aleksandar, Fallas, Jorge A, Sheffler, William, Copps, Jeffrey, Ellis, Daniel, Hutchinson, Geoffrey B, Moyer, Adam, Yasmeen, Anila, Tsybovsky, Yaroslav, Park, Young-Jun, Bick, Matthew J, Sankaran, Banumathi, Gillespie, Rebecca A, Brouwer, Philip Jm, Zwart, Peter H, Veesler, David, Kanekiyo, Masaru, Graham, Barney S, Sanders, Rogier W, Moore, John P, Klasse, Per Johan, Ward, Andrew B, King, Neil P, Baker, David

مصطلحات موضوعية: Humans, Glycoproteins, Influenza Vaccines, Antigens, Viral, Cryoelectron Microscopy, Vaccination, Nanoparticles, Immunity, Humoral, B lymphocytes, BL21, E. coli, HEK293F, Lemo21, computational biology, human, immunology, inflammation, systems biology, virus, Antigens, Viral, Immunity, Humoral, Biochemistry and Cell Biology

وصف الملف: application/pdf

URL الوصول: https://escholarship.org/uc/item/7f44j9rv

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المؤلفون: George Ueda, Aleksandar Antanasijevic, Jorge A Fallas, William Sheffler, Jeffrey Copps, Daniel Ellis, Geoffrey B Hutchinson, Adam Moyer, Anila Yasmeen, Yaroslav Tsybovsky, Young-Jun Park, Matthew J Bick, Banumathi Sankaran, Rebecca A Gillespie, Philip JM Brouwer, Peter H Zwart, David Veesler, Masaru Kanekiyo, Barney S Graham, Rogier W Sanders, John P Moore, Per Johan Klasse, Andrew B Ward, Neil P King, David Baker

المصدر: eLife, Vol 9 (2020)

مصطلحات موضوعية: B lymphocytes, BL21, Lemo21, HEK293F, Medicine, Science, Biology (General), QH301-705.5

وصف الملف: electronic resource

Relation: https://elifesciences.org/articles/57659; https://doaj.org/toc/2050-084X

URL الوصول: https://doaj.org/article/1cb71cd9d73d4231af8ba7577a9734db

المؤلفون: Grietje Kuipers, Alexandros Karyolaimos, Zhe Zhang, Nurzian Ismail, Gianluca Trinco, David Vikström, Dirk Jan Slotboom, Jan-Willem de Gier

المصدر: Microbial Cell Factories, Vol 16, Iss 1, Pp 1-11 (2017)

مصطلحات موضوعية: Escherichia coli, Protein production, Membrane protein, Secretory protein, T7 RNA polymerase, Lemo21(DE3), Microbiology, QR1-502

وصف الملف: electronic resource

Relation: http://link.springer.com/article/10.1186/s12934-017-0840-4; https://doaj.org/toc/1475-2859

URL الوصول: https://doaj.org/article/5461ebc22f7a43058e93cdf812a4c890

المؤلفون: Kuipers, Grietje, Karyolaimos, Alexandros, Zhang, Zhe, Ismail, Nurzian, Trinco, Gianluca, Vikström, David, Slotboom, Dirk Jan, de Gier, Jan-Willem

المصدر: Microbial Cell Factories. 16

مصطلحات موضوعية: Escherichia coli, Protein production, Membrane protein, Secretory protein, T7 RNA polymerase, Lemo21(DE3), Biochemistry, biokemi

وصف الملف: print

URL الوصول: https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-150956
https://doi.org/10.1186/s12934-017-0840-4

المؤلفون: David Veesler, Adam Moyer, Neil P. King, Petrus H. Zwart, George Ueda, Per Johan Klasse, Masaru Kanekiyo, William Sheffler, Jorge A. Fallas, Yaroslav Tsybovsky, David Baker, John P. Moore, Andrew B. Ward, Aleksandar Antanasijevic, Geoffrey B. Hutchinson, Jeffrey Copps, Banumathi Sankaran, Philip J. M. Brouwer, Rogier W. Sanders, Barney S. Graham, Young-Jun Park, Rebecca A. Gillespie, Matthew J. Bick, Anila Yasmeen, Daniel Ellis

المساهمون: Graduate School, AII - Infectious diseases, Medical Microbiology and Infection Prevention

المصدر: eLife, Vol 9 (2020)
eLife
eLife, 9:e57659, 1-30. eLife Sciences Publications

مصطلحات موضوعية: Scaffold, viruses, Nanoparticle, 01 natural sciences, immunology, Immunology and Inflammation, computational biology, Nanotechnology, Viral, Biology (General), Antigens, Viral, chemistry.chemical_classification, 0303 health sciences, biology, Chemistry, General Neuroscience, Vaccination, 030302 biochemistry & molecular biology, Humoral, systems biology, General Medicine, Acquired immune system, 3. Good health, Infectious Diseases, Influenza Vaccines, Pneumonia & Influenza, Medicine, Antibody, Infection, Research Article, Computational and Systems Biology, Icosahedral symmetry, QH301-705.5, Science, Antigen presentation, Hemagglutinin (influenza), Bioengineering, Lemo21, virus, 010402 general chemistry, General Biochemistry, Genetics and Molecular Biology, Virus, Vaccine Related, 03 medical and health sciences, Immune system, Antigen, Humans, human, Antigens, Glycoproteins, 030304 developmental biology, BL21, General Immunology and Microbiology, Prevention, Cryoelectron Microscopy, Immunity, E. coli, Influenza, Immunity, Humoral, 0104 chemical sciences, Emerging Infectious Diseases, Good Health and Well Being, inflammation, HEK293F, biology.protein, Biophysics, Nanoparticles, Immunization, Biochemistry and Cell Biology, Glycoprotein, B lymphocytes

وصف الملف: application/pdf

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5a5b17f68c583d75b01acfb187166331
https://doi.org/10.7554/elife.57659

المؤلفون: Kuipers, Grietje, Peschke, Markus, Ismail, Nurzian Bernsel, Hjelm, Anna, Schlegel, Susan, Vikström, David, Luirink, Joen, de Gier, Jan Willem, Burgess-Brown, Nicola A.

المساهمون: Molecular Microbiology, LaserLaB - Analytical Chemistry and Spectroscopy, AIMMS, Burgess-Brown, Nicola A.

المصدر: Heterologous Gene Expression in E.coli: Methods and Protocols, 109-126
STARTPAGE=109;ENDPAGE=126;TITLE=Heterologous Gene Expression in E.coli
Methods in Molecular Biology ISBN: 9781493968855
Kuipers, G, Peschke, M, Ismail, N B, Hjelm, A, Schlegel, S, Vikström, D, Luirink, J & de Gier, J W 2017, Optimizing E. coli-based membrane protein production using Lemo21(DE3) or pReX and GFP-Fusions . in N A Burgess-Brown (ed.), Heterologous Gene Expression in E.coli : Methods and Protocols . Methods in Molecular Biology, vol. 1586, Humana Press Inc, pp. 109-126 . https://doi.org/10.1007/978-1-4939-6887-9_7

مصطلحات موضوعية: 0301 basic medicine, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, Membrane protein, E. coli, pReX, Production, GFP, 030217 neurology & neurosurgery, Fluorescence, Lemo21(DE3)

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5c502c172ac868f8ed57dad7c7ca3425
https://research.vu.nl/en/publications/972f06d7-ddb7-42bf-8fe8-2098df7bb500

المؤلفون: Gianluca Trinco, Nurzian Bernsel Ismail, Jan-Willem de Gier, David Vikström, Zhe Zhang, Alexandros Karyolaimos, Grietje Kuipers, Dirk Jan Slotboom

المساهمون: Enzymology

المصدر: Microbial Cell Factories
Microbial Cell Factories, 16:226. BioMed Central Ltd.
Microbial Cell Factories, Vol 16, Iss 1, Pp 1-11 (2017)

مصطلحات موضوعية: 0301 basic medicine, GENES, Genetic Vectors, 030106 microbiology, lcsh:QR1-502, Gene Expression, Bioengineering, Applied Microbiology and Biotechnology, lcsh:Microbiology, Viral Proteins, 03 medical and health sciences, Plasmid, Gene expression, Escherichia coli, Protein production, Protein biosynthesis, medicine, T7 RNA polymerase, Cloning, Molecular, GREEN FLUORESCENT PROTEIN, Promoter Regions, Genetic, Gene, Lemo21(DE3), PURIFICATION, Expression vector, BACTERIOPHAGE-T7 RNA-POLYMERASE, Chemistry, Membrane Proteins, DNA-Directed RNA Polymerases, N-Acetylmuramoyl-L-alanine Amidase, Secretory protein, Cell biology, Protein Transport, 030104 developmental biology, Membrane protein, ESCHERICHIA-COLI, bacteria, OVEREXPRESSION, Technical Notes, SYSTEM, Plasmids, Biotechnology, medicine.drug

وصف الملف: application/pdf

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::700b0c83be658c30ae363fb9d71fb3b4

المؤلفون: Hjelm, Anna, Schlegel, Susan, Baumgarten, Thomas, Klepsch, Mirjam, Wickström, David, Drew, David, de Gier, Jan-Willem

المصدر: Membrane Biogenesis Methods in Molecular Biology. :381-400

مصطلحات موضوعية: Membrane protein, Overexpression, Purification, E. coli, Lemo21(DE3), Fluorescence, GFP, FSEC, Biochemistry, biokemi

وصف الملف: print

URL الوصول: https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-96139

المؤلفون: Schlegel, Susan, Rujas, Edurne, Ytterberg, Anders Jimmy, Zubarev, Roman A., Luirink, Joen, de Gier, Jan-Willem

المصدر: Microbial Cell Factories. 12:24

مصطلحات موضوعية: Recombinant protein, Protein production, Escherichia coli, Lemo21(DE3), Protein translocation, Periplasm, Sec-translocon

وصف الملف: print

URL الوصول: https://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-89590
https://doi.org/10.1186/1475-2859-12-24