المؤلفون: Zhao N; Precise Genome Engineering Center, School of Life Sciences, Guangzhou University, Guangzhou, China.; Guangzhou JinHua JiYin() Technology Co., Ltd., Guangzhou, China., Zhou J; Precise Genome Engineering Center, School of Life Sciences, Guangzhou University, Guangzhou, China. zhoujian715412@126.com.; Department of Laboratory Medicines, The First Affiliated Hospital of Xi'an Medical University, Xi'an, China. zhoujian715412@126.com., Tao T; Precise Genome Engineering Center, School of Life Sciences, Guangzhou University, Guangzhou, China., Wang Q; Precise Genome Engineering Center, School of Life Sciences, Guangzhou University, Guangzhou, China., Tang J; Precise Genome Engineering Center, School of Life Sciences, Guangzhou University, Guangzhou, China., Li D; Precise Genome Engineering Center, School of Life Sciences, Guangzhou University, Guangzhou, China., Gou S; Guangzhou National Laboratory, Guangzhou, China., Guan Z; Precise Genome Engineering Center, School of Life Sciences, Guangzhou University, Guangzhou, China., Olajide JS; Precise Genome Engineering Center, School of Life Sciences, Guangzhou University, Guangzhou, China., Lin J; Precise Genome Engineering Center, School of Life Sciences, Guangzhou University, Guangzhou, China., Wang S; Precise Genome Engineering Center, School of Life Sciences, Guangzhou University, Guangzhou, China., Li X; Department of Hepatic Surgery and Liver Transplantation Center of the Third Affiliated Hospital, Organ Transplantation Institute, Sun Yat-sen University, Guangzhou, China., Zhou J; Precise Genome Engineering Center, School of Life Sciences, Guangzhou University, Guangzhou, China., Gao Z; GMU-GIBH Joint School of Life Sciences, The Guangdong-Hong Kong-Macau Joint Laboratory for Cell Fate Regulation and Diseases, Guangzhou Medical University, Guangzhou, China. zgao@gzhmu.edu.cn., Wang G; Precise Genome Engineering Center, School of Life Sciences, Guangzhou University, Guangzhou, China. wanggang.v@gzhu.edu.cn.

المصدر: Nature communications [Nat Commun] 2024 Sep 17; Vol. 15 (1), pp. 8140. Date of Electronic Publication: 2024 Sep 17.

نوع المنشور: Journal Article

بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE

مواضيع طبية MeSH: Gene Editing*/methods , Adenine*/metabolism , Directed Molecular Evolution*/methods , Cytidine*/metabolism , Cytidine*/genetics , CRISPR-Cas Systems* , Cytidine Deaminase*/metabolism , Cytidine Deaminase*/genetics, Humans ; HEK293 Cells ; CRISPR-Associated Protein 9/metabolism ; CRISPR-Associated Protein 9/genetics ; Animals

المؤلفون: Hew BE; Department of Cell and Molecular Biology, Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI 96814, USA., Gupta S; Department of Cell and Molecular Biology, Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI 96814, USA., Sato R; Department of Cell and Molecular Biology, Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI 96814, USA., Waller DF; Department of Cell and Molecular Biology, Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI 96814, USA., Stoytchev I; Department of Cell and Molecular Biology, Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI 96814, USA., Short JE; Department of Cell and Molecular Biology, Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI 96814, USA., Sharek L; Department of Cell and Molecular Biology, Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI 96814, USA., Tran CT; Department of Cell and Molecular Biology, Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI 96814, USA., Badran AH; Department of Chemistry, Department of Integrative Structural and Computational Biology, Beckman Center for Chemical Sciences, The Scripps Research Institute, La Jolla, CA 92037, USA., Owens JB; Department of Cell and Molecular Biology, Institute for Biogenesis Research, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI 96814, USA.

المصدر: Nucleic acids research [Nucleic Acids Res] 2024 Aug 12; Vol. 52 (14), pp. e64.

نوع المنشور: Journal Article

بيانات الدورية: Publisher: Oxford University Press Country of Publication: England NLM ID: 0411011 Publication Model: Print Cited Medium: Internet ISSN: 1362-4962 (Electronic) Linking ISSN: 03051048 NLM ISO Abbreviation: Nucleic Acids Res Subsets: MEDLINE

مواضيع طبية MeSH: Directed Molecular Evolution*/methods , Transgenes* , Integrases*/metabolism , Integrases*/genetics, Humans ; Mutagenesis, Insertional ; Mutation ; Bacteriophages/genetics ; Bacteriophages/enzymology ; Attachment Sites, Microbiological/genetics

المؤلفون: Chen A; School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts, 02138, USA E-mail: Dr David A. Weitz: E-mail: Dr. Karla Milcic., Zhang XD; School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts, 02138, USA E-mail: Dr David A. Weitz: E-mail: Dr. Karla Milcic., Đelmaš AĐ; University of Belgrade-Faculty of Chemistry, Studentski trg 12-16, 11000, Belgrade, Serbia., Weitz DA; School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts, 02138, USA E-mail: Dr David A. Weitz: E-mail: Dr. Karla Milcic.; Wyss Institute for Biologically Inspired Engineering, Harvard University, 3 Blackfan Circle, Boston, MA, 02115, USA.; Department of Physics, Harvard University, Cambridge, MA, 02138, USA., Milcic K; School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts, 02138, USA E-mail: Dr David A. Weitz: E-mail: Dr. Karla Milcic.; University of Belgrade-Faculty of Chemistry, Studentski trg 12-16, 11000, Belgrade, Serbia.

المصدر: Chemistry (Weinheim an der Bergstrasse, Germany) [Chemistry] 2024 Aug 01; Vol. 30 (43), pp. e202400880. Date of Electronic Publication: 2024 Jul 12.

نوع المنشور: Journal Article; Review

بيانات الدورية: Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 9513783 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1521-3765 (Electronic) Linking ISSN: 09476539 NLM ISO Abbreviation: Chemistry Subsets: MEDLINE

مواضيع طبية MeSH: Enzymes*/metabolism , Enzymes*/chemistry , Enzymes*/genetics , Directed Molecular Evolution*/methods , Protein Engineering*, Biocatalysis ; Humans

المؤلفون: Xu B; School of Basic Medical Sciences, Hubei University of Science and Technology, Xianning, 437100, P. R. China., Liu LH; Tidetron Bioworks Technology (Guangzhou) Co., Ltd., Guangzhou Qianxiang Bioworks Co., Ltd, Guangzhou, Guangdong, 510000, P. R. China.; Biology Department and Institute of Marine Sciences, College of Science, Shantou University, Shantou, 515063, P. R. China., Lai S; Tidetron Bioworks Technology (Guangzhou) Co., Ltd., Guangzhou Qianxiang Bioworks Co., Ltd, Guangzhou, Guangdong, 510000, P. R. China., Chen J; Yeasen Biotechnology (Shanghai) Co., Ltd, Shanghai, 200000, P. R. China., Wu S; Tidetron Bioworks Technology (Guangzhou) Co., Ltd., Guangzhou Qianxiang Bioworks Co., Ltd, Guangzhou, Guangdong, 510000, P. R. China., Lei W; Tidetron Bioworks Technology (Guangzhou) Co., Ltd., Guangzhou Qianxiang Bioworks Co., Ltd, Guangzhou, Guangdong, 510000, P. R. China., Lin H; Tidetron Bioworks Technology (Guangzhou) Co., Ltd., Guangzhou Qianxiang Bioworks Co., Ltd, Guangzhou, Guangdong, 510000, P. R. China., Zhang Y; Tidetron Bioworks Technology (Guangzhou) Co., Ltd., Guangzhou Qianxiang Bioworks Co., Ltd, Guangzhou, Guangdong, 510000, P. R. China., Hu Y; Tidetron Bioworks Technology (Guangzhou) Co., Ltd., Guangzhou Qianxiang Bioworks Co., Ltd, Guangzhou, Guangdong, 510000, P. R. China.; College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, P. R. China., He J; Tidetron Bioworks Technology (Guangzhou) Co., Ltd., Guangzhou Qianxiang Bioworks Co., Ltd, Guangzhou, Guangdong, 510000, P. R. China., Chen X; Tidetron Bioworks Technology (Guangzhou) Co., Ltd., Guangzhou Qianxiang Bioworks Co., Ltd, Guangzhou, Guangdong, 510000, P. R. China., He Q; Tidetron Bioworks Technology (Guangzhou) Co., Ltd., Guangzhou Qianxiang Bioworks Co., Ltd, Guangzhou, Guangdong, 510000, P. R. China., Yang M; Tidetron Bioworks Technology (Guangzhou) Co., Ltd., Guangzhou Qianxiang Bioworks Co., Ltd, Guangzhou, Guangdong, 510000, P. R. China., Wang H; Tidetron Bioworks Technology (Guangzhou) Co., Ltd., Guangzhou Qianxiang Bioworks Co., Ltd, Guangzhou, Guangdong, 510000, P. R. China., Zhao X; Tidetron Bioworks Technology (Guangzhou) Co., Ltd., Guangzhou Qianxiang Bioworks Co., Ltd, Guangzhou, Guangdong, 510000, P. R. China., Wang M; Yeasen Biotechnology (Shanghai) Co., Ltd, Shanghai, 200000, P. R. China., Luo H; Tidetron Bioworks Technology (Guangzhou) Co., Ltd., Guangzhou Qianxiang Bioworks Co., Ltd, Guangzhou, Guangdong, 510000, P. R. China.; Biology Department and Institute of Marine Sciences, College of Science, Shantou University, Shantou, 515063, P. R. China., Ge Q; Tidetron Bioworks Technology (Guangzhou) Co., Ltd., Guangzhou Qianxiang Bioworks Co., Ltd, Guangzhou, Guangdong, 510000, P. R. China., Gao H; Tidetron Bioworks Technology (Guangzhou) Co., Ltd., Guangzhou Qianxiang Bioworks Co., Ltd, Guangzhou, Guangdong, 510000, P. R. China., Xia J; School of Basic Medicine, Jiamusi University, Jiamusi, 154000, P. R. China., Cao Z; Yeasen Biotechnology (Shanghai) Co., Ltd, Shanghai, 200000, P. R. China., Zhang B; College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, P. R. China., Jiang A; Tidetron Bioworks Technology (Guangzhou) Co., Ltd., Guangzhou Qianxiang Bioworks Co., Ltd, Guangzhou, Guangdong, 510000, P. R. China., Wu YR; Tidetron Bioworks Technology (Guangzhou) Co., Ltd., Guangzhou Qianxiang Bioworks Co., Ltd, Guangzhou, Guangdong, 510000, P. R. China.

المصدر: Small methods [Small Methods] 2024 Aug; Vol. 8 (8), pp. e2301385. Date of Electronic Publication: 2024 Feb 28.

نوع المنشور: Journal Article

بيانات الدورية: Publisher: WILEY-VCH Verlag GmbH & Co. KGaA Country of Publication: Germany NLM ID: 101724536 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2366-9608 (Electronic) Linking ISSN: 23669608 NLM ISO Abbreviation: Small Methods Subsets: MEDLINE

مواضيع طبية MeSH: Escherichia coli*/genetics , Escherichia coli*/metabolism , Directed Molecular Evolution*/methods, Fructose/metabolism ; Carbon/metabolism ; Mutation ; Escherichia coli Proteins/genetics ; Escherichia coli Proteins/metabolism ; Whole Genome Sequencing ; Metabolic Networks and Pathways

المؤلفون: Laurent JM; Department of Materials, Complex Materials, ETH Zürich, Zürich 8093, Switzerland., Jain A; Department of Chemistry and Applied Biosciences, Institute for Chemical and Bioengineering, ETH Zürich, Zürich 8093, Switzerland., Kan A; Department of Materials, Complex Materials, ETH Zürich, Zürich 8093, Switzerland., Steinacher M; Department of Materials, Complex Materials, ETH Zürich, Zürich 8093, Switzerland., Enrriquez Casimiro N; Department of Materials, Complex Materials, ETH Zürich, Zürich 8093, Switzerland., Stavrakis S; Department of Chemistry and Applied Biosciences, Institute for Chemical and Bioengineering, ETH Zürich, Zürich 8093, Switzerland., deMello AJ; Department of Chemistry and Applied Biosciences, Institute for Chemical and Bioengineering, ETH Zürich, Zürich 8093, Switzerland., Studart AR; Department of Materials, Complex Materials, ETH Zürich, Zürich 8093, Switzerland.

المصدر: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2024 Jul 30; Vol. 121 (31), pp. e2403585121. Date of Electronic Publication: 2024 Jul 23.

نوع المنشور: Journal Article

بيانات الدورية: Publisher: National Academy of Sciences Country of Publication: United States NLM ID: 7505876 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1091-6490 (Electronic) Linking ISSN: 00278424 NLM ISO Abbreviation: Proc Natl Acad Sci U S A Subsets: MEDLINE

مواضيع طبية MeSH: Cellulose*/metabolism , Cellulose*/biosynthesis , Directed Molecular Evolution*/methods, Acetobacteraceae/metabolism ; Acetobacteraceae/genetics ; Phenotype ; Mutation

المؤلفون: Ding K; School of Computational Science and Engineering, Georgia Institute of Technology, Atlanta, GA, 30332, USA., Chin M; Department of Chemistry and Biochemistry, University of California, Santa Barbara, CA, 93106, USA., Zhao Y; Department of Chemistry and Biochemistry, University of California, Santa Barbara, CA, 93106, USA., Huang W; Department of Chemistry and Biochemistry, University of California, Santa Barbara, CA, 93106, USA., Mai BK; Department of Chemistry, University of Pittsburgh, Pittsburgh, PA, 15260, USA., Wang H; Department of Chemistry and Biochemistry, University of California, Santa Barbara, CA, 93106, USA., Liu P; Department of Chemistry, University of Pittsburgh, Pittsburgh, PA, 15260, USA. pengliu@pitt.edu., Yang Y; Department of Chemistry and Biochemistry, University of California, Santa Barbara, CA, 93106, USA. yang@chem.ucsb.edu.; Biomolecular Science and Engineering (BMSE) Program, University of California, Santa Barbara, CA, 93106, USA. yang@chem.ucsb.edu., Luo Y; School of Computational Science and Engineering, Georgia Institute of Technology, Atlanta, GA, 30332, USA. yunan@gatech.edu.

المصدر: Nature communications [Nat Commun] 2024 Jul 29; Vol. 15 (1), pp. 6392. Date of Electronic Publication: 2024 Jul 29.

نوع المنشور: Journal Article

بيانات الدورية: Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: MEDLINE

مواضيع طبية MeSH: Protein Engineering*/methods , Machine Learning* , Directed Molecular Evolution*/methods , Mutation*, Biocatalysis ; Algorithms ; Gene Library ; Enzymes/metabolism ; Enzymes/genetics ; Enzymes/chemistry

المؤلفون: Hurt RC, Jin Z, Soufi M, Wong KK, Sawyer DP, Shen HK, Dutka P, Deshpande R, Zhang R, Mittelstein DR, Shapiro MG

المصدر: ACS synthetic biology [ACS Synth Biol] 2024 Jul 19; Vol. 13 (7), pp. 2215-2226. Date of Electronic Publication: 2024 Jul 09.

نوع المنشور: Journal Article

بيانات الدورية: Publisher: American Chemical Society Country of Publication: United States NLM ID: 101575075 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2161-5063 (Electronic) Linking ISSN: 21615063 NLM ISO Abbreviation: ACS Synth Biol Subsets: MEDLINE

مواضيع طبية MeSH: Directed Molecular Evolution*/methods , Genes, Reporter*, High-Throughput Screening Assays/methods ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Acoustics ; Nanostructures/chemistry

المؤلفون: Huang RL; Department of Chemistry, Boston College, Chestnut Hill, Massachusetts 02467, United States., Jewel D; Department of Chemistry, Boston College, Chestnut Hill, Massachusetts 02467, United States., Kelemen RE; Department of Chemistry, Boston College, Chestnut Hill, Massachusetts 02467, United States., Pham Q; Department of Chemistry, Boston College, Chestnut Hill, Massachusetts 02467, United States., Yared TJ; Department of Chemistry, Boston College, Chestnut Hill, Massachusetts 02467, United States., Wang S; Department of Chemistry, Boston College, Chestnut Hill, Massachusetts 02467, United States., Singha Roy SJ; Department of Chemistry, Boston College, Chestnut Hill, Massachusetts 02467, United States., Huang Z; Department of Chemistry, Boston College, Chestnut Hill, Massachusetts 02467, United States., Levinson SD; Department of Chemistry, Boston College, Chestnut Hill, Massachusetts 02467, United States., Sundaresh B; Biology Department, Boston College, Chestnut Hill, Massachusetts 02467, United States., Miranda SE; Biology Department, Boston College, Chestnut Hill, Massachusetts 02467, United States., van Opijnen T; Biology Department, Boston College, Chestnut Hill, Massachusetts 02467, United States.; Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, United States., Chatterjee A; Department of Chemistry, Boston College, Chestnut Hill, Massachusetts 02467, United States.

المصدر: ACS synthetic biology [ACS Synth Biol] 2024 Jul 19; Vol. 13 (7), pp. 2141-2149. Date of Electronic Publication: 2024 Jun 21.

نوع المنشور: Journal Article

بيانات الدورية: Publisher: American Chemical Society Country of Publication: United States NLM ID: 101575075 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2161-5063 (Electronic) Linking ISSN: 21615063 NLM ISO Abbreviation: ACS Synth Biol Subsets: MEDLINE

مواضيع طبية MeSH: Directed Molecular Evolution*/methods , Escherichia coli*/genetics , Escherichia coli*/metabolism , Amino Acids*/genetics , Amino Acids*/metabolism , Mutagenesis*, Humans ; HEK293 Cells ; Leucine-tRNA Ligase/genetics ; Leucine-tRNA Ligase/metabolism

المؤلفون: Shanker VR; Stanford Biophysics Program, Stanford University School of Medicine, Stanford, CA 94305, USA.; Stanford Medical Scientist Training Program, Stanford University School of Medicine, Stanford, CA 94305, USA.; Sarafan ChEM-H, Stanford University, Stanford, CA 94305, USA., Bruun TUJ; Stanford Medical Scientist Training Program, Stanford University School of Medicine, Stanford, CA 94305, USA.; Sarafan ChEM-H, Stanford University, Stanford, CA 94305, USA.; Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA., Hie BL; Sarafan ChEM-H, Stanford University, Stanford, CA 94305, USA.; Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA., Kim PS; Sarafan ChEM-H, Stanford University, Stanford, CA 94305, USA.; Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305, USA.; Chan Zuckerberg Biohub, San Francisco, CA 94158, USA.

المصدر: Science (New York, N.Y.) [Science] 2024 Jul 05; Vol. 385 (6704), pp. 46-53. Date of Electronic Publication: 2024 Jul 04.

نوع المنشور: Journal Article

بيانات الدورية: Publisher: American Association for the Advancement of Science Country of Publication: United States NLM ID: 0404511 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-9203 (Electronic) Linking ISSN: 00368075 NLM ISO Abbreviation: Science Subsets: MEDLINE

مواضيع طبية MeSH: Antibodies, Neutralizing*/chemistry , Antibodies, Neutralizing*/genetics , Antibodies, Neutralizing*/immunology , Antibodies, Viral*/chemistry , Antibodies, Viral*/genetics , Antibodies, Viral*/immunology , Directed Molecular Evolution*/methods, Humans ; Antibody Affinity ; Antigen-Antibody Complex/chemistry ; COVID-19/virology ; COVID-19/immunology ; Models, Molecular ; Protein Conformation ; Protein Engineering ; SARS-CoV-2/immunology ; SARS-CoV-2/genetics

SCR Organism: SARS-CoV-2 variants

المؤلفون: Zhu H; State Key Laboratory of Genetic Engineering, Shanghai Engineering Research Center of Industrial Microorganisms, MOE Engineering Research Center of Gene Technology, School of Life Sciences, Fudan University, Shanghai, 200438, China., Wang L; State Key Laboratory of Genetic Engineering, Shanghai Engineering Research Center of Industrial Microorganisms, MOE Engineering Research Center of Gene Technology, School of Life Sciences, Fudan University, Shanghai, 200438, China., Wang Y; State Key Laboratory of Genetic Engineering, Shanghai Engineering Research Center of Industrial Microorganisms, MOE Engineering Research Center of Gene Technology, School of Life Sciences, Fudan University, Shanghai, 200438, China., Jiang X; State Key Laboratory of Genetic Engineering, Shanghai Engineering Research Center of Industrial Microorganisms, MOE Engineering Research Center of Gene Technology, School of Life Sciences, Fudan University, Shanghai, 200438, China., Qin Q; State Key Laboratory of Genetic Engineering, Shanghai Engineering Research Center of Industrial Microorganisms, MOE Engineering Research Center of Gene Technology, School of Life Sciences, Fudan University, Shanghai, 200438, China., Song M; State Key Laboratory of Genetic Engineering, Shanghai Engineering Research Center of Industrial Microorganisms, MOE Engineering Research Center of Gene Technology, School of Life Sciences, Fudan University, Shanghai, 200438, China., Huang Q; State Key Laboratory of Genetic Engineering, Shanghai Engineering Research Center of Industrial Microorganisms, MOE Engineering Research Center of Gene Technology, School of Life Sciences, Fudan University, Shanghai, 200438, China. huangqiang@fudan.edu.cn.; Multiscale Research Institute of Complex Systems, Fudan University, Shanghai, 201203, China. huangqiang@fudan.edu.cn.

المصدر: Cellular and molecular life sciences : CMLS [Cell Mol Life Sci] 2024 Jun 14; Vol. 81 (1), pp. 257. Date of Electronic Publication: 2024 Jun 14.

نوع المنشور: Journal Article

بيانات الدورية: Publisher: Springer Country of Publication: Switzerland NLM ID: 9705402 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-9071 (Electronic) Linking ISSN: 1420682X NLM ISO Abbreviation: Cell Mol Life Sci Subsets: MEDLINE

مواضيع طبية MeSH: DNA*/metabolism , DNA*/genetics , DNA*/chemistry , Mutation* , Molecular Dynamics Simulation* , Directed Molecular Evolution* , Gene Editing*/methods, Adenine/metabolism ; Adenine/chemistry ; Protein Stability ; Protein Binding ; Static Electricity ; CRISPR-Cas Systems/genetics