المؤلفون: Pimchai Chaiyen, Jirawat Tantipisit, Asweena Binlaeh, Litavadee Chuaboon, Juthamas Jaroensuk, Somchart Maenpuen, Penchit Chitnumsub, Ruchanok Tinikul, Aritsara Jaruwat, Jittima Phonbuppha, Pratchaya Watthaisong, Narin Lawan

المصدر: The Journal of Biological Chemistry

مصطلحات موضوعية: structure–function, p-hydroxyphenylacetate degradation pathway, Acinetobacter baumannii, Enzyme complex, PMSF, phenyl methane sulfonyl fluoride, QM/MM, quantum mechanics/molecular mechanics, DHAP, dihydroxyacetone phosphate, SSA, succinic semialdehyde, Crystallography, X-Ray, Biochemistry, HKHD, 4-hydroxy-2-ketoheptane-1,7-dioate, MW, molecular weight, Substrate Specificity, FPLC, fast protein liquid chromatography, LC-ESI-QTOF-MS, liquid chromatography-electrospray ionization-quadrupole-time-of-flight mass spectrometer, NaCl, sodium chloride, Aldol reaction, stereospecificity, Catalytic Domain, Fructose-Bisphosphate Aldolase, Enzyme Stability, Tm, melting temperature, PPA, propionaldehyde, biology, solvent-tolerant enzyme, LDH, lactate dehydrogenase, Chemistry, MD, molecular dynamics, Enzyme structure, HOPA, 4-hydroxy-2-oxopentanoate, Zinc, (NH4)2SO4, ammonium sulfate, PYR, pyruvate, metal-dependent enzyme, Research Article, crystal structure, HNO3, nitric acid, SEC, size-exclusion chromatography, ICP-OES, inductively coupled plasma-optical emission spectrometry, Stereochemistry, (4R)-KDGal, (4R)-2-keto-3-deoxy-D-galactonate, stereoselectivity, enzyme catalysis, Catalysis, Enzyme catalysis, Stereospecificity, (4S)-KDGlu, (4S)-2-keto-3-deoxy-D-gluconate, Bacterial Proteins, PDB, Protein Data Bank, NADH, the reduced β-nicotinamide adenine dinucleotide, thermostable enzyme, Molecular Biology, M2+, divalent meatl ion, PEI, polyethyleneimine, MPD, 2-methyl-2,4-pentanediol, Aldolase A, EGTA, ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid, HEPES, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, Cell Biology, AbHpaI, 4-hydroxy-2-ketoheptane-1,7-dioate aldolase from Acinetobacter baumannii, HBA, 4-hydroxybenzaldehyde, EcHpaI, 4-hydroxy-2-ketoheptane-1,7-dioate aldolase from Escherichia coli, Biocatalysis, DTT, dithiothreitol, biology.protein, Aldol condensation, BSA, bovine serum albumin, EDTA, ethylenediaminetetraacetatic acid, Calcium, OAA, oxaloacetate, pyruvate-specific Class II metal aldolase

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::69ec40387511a3835e3f518a5fda0050
http://europepmc.org/articles/PMC8560999