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المؤلفون: D'Onofrio, F., Schirone, M., Lolli, M. G., Sacchini, F., Krasteva, I., DI Febo, T., Pomilio, F., Centorame, P., Tittarelli, M., Luciani, M., Iannetti, L.
مصطلحات موضوعية: The following study aims to compare Listeria monocytogenes enumeration performed by ISO 11290-2 and flow cytometry. Experiments were carried out on two L. monocytogenes strains grown at 8 and 4°C. The results highlighted that flow cytometry technique did not count the total number of bacterial cells enumerated by the official technique. Nevertheless it was evinced a constant relation between values obtained with both techniques: starting from a value of 4 log CFU/ml, flow cytometry permitted to enumerate 90-95% of the total L. monocytogenes cells counted by ISO 11290-2. This percentage decreased to 85-90% for 5 log CFU/ml, 65-70% for 6 log UFC/ml, The following study aims to compare Listeria monocytogenes enumeration performed by ISO 11290-2 and flow cytometry. Experiments were carried out on two L. monocytogenes strains grown at 8 and 4°C. The results highlighted that flow cytometry technique did not count the total number of bacterial cells enumerated by the official technique. Nevertheless it was evinced a constant relation between values obtained with both techniques: starting from a value of 4 log CFU/ml, flow cytometry permitted to enumerate 90-95% of the total L. monocytogenes cells counted by ISO 11290-2. This percentage decreased to 85-90% for 5 log CFU/ml, 65-70% for 6 log UFC/ml, 60-65% for 7 log CFU/ml, and 55-60% for 8 log CFU/ml. No significant differences of the enumeration were observed as function of strains and growth temperature, and 55-60% for 8 log CFU/ml. No significant differences of the enumeration were observed as function of strains and growth temperature, 60-65% for 7 log CFU/ml
URL الوصول: https://explore.openaire.eu/search/publication?articleId=od______3664::5a7b54ed392c5c6bb2c0cc25502b059a
http://hdl.handle.net/11575/123958 -
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