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1
المؤلفون: Catherine L. Madinger, Michelle L. Cushing, Julie Canovas, Christopher H. Taron, Jack S. Benner, Brian P. Anton, Lauren G. Fields, Shamik S. Sharma
المصدر: PROTEOMICS. 9:4744-4754
مصطلحات موضوعية: Kluyveromyces lactis, Glycosylation, Proteome, biology, medicine.diagnostic_test, Proteolysis, Computational Biology, Proteomics, biology.organism_classification, Biochemistry, Carbon, Fungal Proteins, Kluyveromyces, chemistry.chemical_compound, Secretory protein, stomatognathic system, chemistry, Galactose, medicine, Molecular Biology, Secretory pathway
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2
المؤلفون: Christopher H. Taron, Jack S. Benner, Shamik S. Sharma, Brian P. Anton, Catherine L. Swaim
المصدر: PROTEOMICS. 8:2714-2723
مصطلحات موضوعية: Proteomics, Kluyveromyces lactis, Proteome, biology, In silico, Genes, Fungal, Computational Biology, biology.organism_classification, Models, Biological, Biochemistry, Yeast, Culture Media, Fungal Proteins, Kluyveromyces, Bioreactors, Secretory protein, Gene Expression Regulation, Fungal, Fermentation, Computer Simulation, Secretion, Molecular Biology, Secretory pathway
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3
المؤلفون: Sarah W. Harcum, Shamik S. Sharma, Frederick R. Blattner
المصدر: Metabolic Engineering. 9:133-141
مصطلحات موضوعية: Transposable element, Bioengineering, Biology, Protein Engineering, medicine.disease_cause, Applied Microbiology and Biotechnology, Genome, Article, law.invention, Bacterial genetics, Species Specificity, law, Escherichia coli, medicine, Insertion sequence, Gene, Cell Proliferation, Strain (chemistry), Escherichia coli Proteins, Molecular biology, Recombinant Proteins, Genetic Enhancement, Gene Targeting, Recombinant DNA, Gene Deletion, Genome, Bacterial, Biotechnology
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4
المؤلفون: Shamik S. Sharma, David Frisch, John Walter Campbell, Sarah W. Harcum, Frederick R. Blattner
المصدر: Biotechnology and Bioengineering. 98:1056-1070
مصطلحات موضوعية: Chloramphenicol O-Acetyltransferase, Glycerol, Isopropyl Thiogalactoside, Transposable element, Gene Expression, Cell Count, Bioengineering, Acetates, Biology, medicine.disease_cause, Applied Microbiology and Biotechnology, Genome, law.invention, Chloramphenicol acetyltransferase, Phosphate Acetyltransferase, Acetyl Coenzyme A, law, Escherichia coli, medicine, Biomass, Insertion sequence, Promoter Regions, Genetic, Gene, Prophage, Genetics, Recombinant Proteins, Oxygen, Glucose, Biochemistry, Recombinant DNA, Glycolysis, Gene Deletion, Genome, Bacterial, Plasmids, Biotechnology
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5
المؤلفون: Sarah W. Harcum, Shaorong Chong, Shamik S. Sharma
المصدر: Applied Biochemistry and Biotechnology. 126:093-118
مصطلحات موضوعية: Reducing agent, Recombinant Fusion Proteins, Chitin, Bioengineering, Biology, medicine.disease_cause, Applied Microbiology and Biotechnology, Biochemistry, Inteins, law.invention, Industrial Microbiology, chemistry.chemical_compound, Affinity chromatography, law, Escherichia coli, medicine, Computer Simulation, Tromethamine, Molecular Biology, Temperature, General Medicine, Hydrogen-Ion Concentration, Fusion protein, Molecular Weight, carbohydrates (lipids), Dithiothreitol, Solubility, chemistry, Recombinant DNA, Target protein, Intein, Software, Biotechnology
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6
المؤلفون: Shaorong Chong, Sarah W. Harcum, Aihua Zhang, Shamik S. Sharma, Haoyong Wang
المصدر: Biotechnology Progress. 19:1085-1090
مصطلحات موضوعية: Recombinant Fusion Proteins, Cell Culture Techniques, Cell Count, Gene Expression Regulation, Bacterial, Protein tag, Biology, Protein Engineering, Fusion protein, Industrial Microbiology, Proto-Oncogene Proteins c-myb, Bioreactors, Biochemistry, Affinity chromatography, Protein splicing, Protein purification, RNA splicing, Escherichia coli, Protein Splicing, Peptide bond, Intein, Biotechnology
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7
المؤلفون: David Frisch, Guy Plunkett, Shamik S. Sharma, Buffy Stahl, György Pósfai, Vitaliy Kolisnychenko, Kinga Umenhoffer, Günther M. Keil, Monika de Arruda, Valerie Burland, Tamás Fehér, Frederick R. Blattner, Sarah W. Harcum
المصدر: Science. 312:1044-1046
مصطلحات موضوعية: DNA, Bacterial, Genetics, Mutation, Multidisciplinary, Escherichia coli K12, Mutagenesis (molecular biology technique), Virulence, Biology, medicine.disease_cause, Genome, Synthetic biology, Plasmid, Species Specificity, Mutagenesis, DNA Transposable Elements, medicine, Genetic Engineering, Escherichia coli, Gene, Gene Deletion, Genome, Bacterial, Plasmids
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b3dc51f6d0920d9bf47e81233aabad43
https://doi.org/10.1126/science.1126439 -
8
المؤلفون: Sarah W. Harcum, Shamik S. Sharma, Shaorong Chong
المصدر: Journal of biotechnology. 125(1)
مصطلحات موضوعية: Tandem affinity purification, Integrases, Recombinant Fusion Proteins, Reproducibility of Results, Bioengineering, General Medicine, Biology, Applied Microbiology and Biotechnology, Fusion protein, Chromatography, Affinity, Inteins, Fibroblast Growth Factors, Affinity chromatography, FLAG-tag, Biochemistry, Protein splicing, alpha 1-Antitrypsin, Protein purification, Escherichia coli, Peptide bond, Humans, Protein Splicing, Intein, Cell Division, Biotechnology
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9دورية أكاديمية
المؤلفون: Sharma S; New England Biolabs, 32 Tozer Road, Beverly, Massachusetts 01915, USA., Zhang A, Wang H, Harcum SW, Chong S
المصدر: Biotechnology progress [Biotechnol Prog] 2003 May-Jun; Vol. 19 (3), pp. 1085-90.
نوع المنشور: Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.
بيانات الدورية: Publisher: Wiley-Blackwell Country of Publication: United States NLM ID: 8506292 Publication Model: Print Cited Medium: Print ISSN: 8756-7938 (Print) Linking ISSN: 15206033 NLM ISO Abbreviation: Biotechnol Prog Subsets: MEDLINE
مواضيع طبية MeSH: Bioreactors*/*microbiology, Protein Engineering/*methods , Protein Splicing/*physiology , Proto-Oncogene Proteins c-myb/*biosynthesis , Proto-Oncogene Proteins c-myb/*isolation & purification , Recombinant Fusion Proteins/*biosynthesis , Recombinant Fusion Proteins/*isolation & purification, Cell Count ; Cell Culture Techniques/methods ; Escherichia coli/genetics ; Escherichia coli/growth & development ; Escherichia coli/metabolism ; Gene Expression Regulation, Bacterial ; Industrial Microbiology/methods ; Proto-Oncogene Proteins c-myb/genetics ; Recombinant Fusion Proteins/genetics