المؤلفون: Catherine L. Madinger, Michelle L. Cushing, Julie Canovas, Christopher H. Taron, Jack S. Benner, Brian P. Anton, Lauren G. Fields, Shamik S. Sharma

المصدر: PROTEOMICS. 9:4744-4754

مصطلحات موضوعية: Kluyveromyces lactis, Glycosylation, Proteome, biology, medicine.diagnostic_test, Proteolysis, Computational Biology, Proteomics, biology.organism_classification, Biochemistry, Carbon, Fungal Proteins, Kluyveromyces, chemistry.chemical_compound, Secretory protein, stomatognathic system, chemistry, Galactose, medicine, Molecular Biology, Secretory pathway

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a3d41b29c965c21fd41baa3bacdb8a3d
https://doi.org/10.1002/pmic.200800915

المؤلفون: Christopher H. Taron, Jack S. Benner, Shamik S. Sharma, Brian P. Anton, Catherine L. Swaim

المصدر: PROTEOMICS. 8:2714-2723

مصطلحات موضوعية: Proteomics, Kluyveromyces lactis, Proteome, biology, In silico, Genes, Fungal, Computational Biology, biology.organism_classification, Models, Biological, Biochemistry, Yeast, Culture Media, Fungal Proteins, Kluyveromyces, Bioreactors, Secretory protein, Gene Expression Regulation, Fungal, Fermentation, Computer Simulation, Secretion, Molecular Biology, Secretory pathway

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3097a7ac4995a67f048a3bb9efc64b89
https://doi.org/10.1002/pmic.200700764

المؤلفون: Sarah W. Harcum, Shamik S. Sharma, Frederick R. Blattner

المصدر: Metabolic Engineering. 9:133-141

مصطلحات موضوعية: Transposable element, Bioengineering, Biology, Protein Engineering, medicine.disease_cause, Applied Microbiology and Biotechnology, Genome, Article, law.invention, Bacterial genetics, Species Specificity, law, Escherichia coli, medicine, Insertion sequence, Gene, Cell Proliferation, Strain (chemistry), Escherichia coli Proteins, Molecular biology, Recombinant Proteins, Genetic Enhancement, Gene Targeting, Recombinant DNA, Gene Deletion, Genome, Bacterial, Biotechnology

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d696af0266c0ea076551ea15204fc38a
https://doi.org/10.1016/j.ymben.2006.10.002

المؤلفون: Shamik S. Sharma, David Frisch, John Walter Campbell, Sarah W. Harcum, Frederick R. Blattner

المصدر: Biotechnology and Bioengineering. 98:1056-1070

مصطلحات موضوعية: Chloramphenicol O-Acetyltransferase, Glycerol, Isopropyl Thiogalactoside, Transposable element, Gene Expression, Cell Count, Bioengineering, Acetates, Biology, medicine.disease_cause, Applied Microbiology and Biotechnology, Genome, law.invention, Chloramphenicol acetyltransferase, Phosphate Acetyltransferase, Acetyl Coenzyme A, law, Escherichia coli, medicine, Biomass, Insertion sequence, Promoter Regions, Genetic, Gene, Prophage, Genetics, Recombinant Proteins, Oxygen, Glucose, Biochemistry, Recombinant DNA, Glycolysis, Gene Deletion, Genome, Bacterial, Plasmids, Biotechnology

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2bfbfa3d2df02ec97fedce7e09744fa2
https://doi.org/10.1002/bit.21491

المؤلفون: Sarah W. Harcum, Shaorong Chong, Shamik S. Sharma

المصدر: Applied Biochemistry and Biotechnology. 126:093-118

مصطلحات موضوعية: Reducing agent, Recombinant Fusion Proteins, Chitin, Bioengineering, Biology, medicine.disease_cause, Applied Microbiology and Biotechnology, Biochemistry, Inteins, law.invention, Industrial Microbiology, chemistry.chemical_compound, Affinity chromatography, law, Escherichia coli, medicine, Computer Simulation, Tromethamine, Molecular Biology, Temperature, General Medicine, Hydrogen-Ion Concentration, Fusion protein, Molecular Weight, carbohydrates (lipids), Dithiothreitol, Solubility, chemistry, Recombinant DNA, Target protein, Intein, Software, Biotechnology

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7e5953ed542115475568e7b0d3f0ba5e
https://doi.org/10.1385/abab:126:2:093

المؤلفون: Shaorong Chong, Sarah W. Harcum, Aihua Zhang, Shamik S. Sharma, Haoyong Wang

المصدر: Biotechnology Progress. 19:1085-1090

مصطلحات موضوعية: Recombinant Fusion Proteins, Cell Culture Techniques, Cell Count, Gene Expression Regulation, Bacterial, Protein tag, Biology, Protein Engineering, Fusion protein, Industrial Microbiology, Proto-Oncogene Proteins c-myb, Bioreactors, Biochemistry, Affinity chromatography, Protein splicing, Protein purification, RNA splicing, Escherichia coli, Protein Splicing, Peptide bond, Intein, Biotechnology

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::12964589eefd44bef36449e8f30205c0
https://doi.org/10.1021/bp034009r

المؤلفون: David Frisch, Guy Plunkett, Shamik S. Sharma, Buffy Stahl, György Pósfai, Vitaliy Kolisnychenko, Kinga Umenhoffer, Günther M. Keil, Monika de Arruda, Valerie Burland, Tamás Fehér, Frederick R. Blattner, Sarah W. Harcum

المصدر: Science. 312:1044-1046

مصطلحات موضوعية: DNA, Bacterial, Genetics, Mutation, Multidisciplinary, Escherichia coli K12, Mutagenesis (molecular biology technique), Virulence, Biology, medicine.disease_cause, Genome, Synthetic biology, Plasmid, Species Specificity, Mutagenesis, DNA Transposable Elements, medicine, Genetic Engineering, Escherichia coli, Gene, Gene Deletion, Genome, Bacterial, Plasmids

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b3dc51f6d0920d9bf47e81233aabad43
https://doi.org/10.1126/science.1126439

المؤلفون: Sarah W. Harcum, Shamik S. Sharma, Shaorong Chong

المصدر: Journal of biotechnology. 125(1)

مصطلحات موضوعية: Tandem affinity purification, Integrases, Recombinant Fusion Proteins, Reproducibility of Results, Bioengineering, General Medicine, Biology, Applied Microbiology and Biotechnology, Fusion protein, Chromatography, Affinity, Inteins, Fibroblast Growth Factors, Affinity chromatography, FLAG-tag, Biochemistry, Protein splicing, alpha 1-Antitrypsin, Protein purification, Escherichia coli, Peptide bond, Humans, Protein Splicing, Intein, Cell Division, Biotechnology

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d817a2f6aac5c176515a5459efaa5d33
https://pubmed.ncbi.nlm.nih.gov/16546284

المؤلفون: Sharma S; New England Biolabs, 32 Tozer Road, Beverly, Massachusetts 01915, USA., Zhang A, Wang H, Harcum SW, Chong S

المصدر: Biotechnology progress [Biotechnol Prog] 2003 May-Jun; Vol. 19 (3), pp. 1085-90.

نوع المنشور: Evaluation Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.

بيانات الدورية: Publisher: Wiley-Blackwell Country of Publication: United States NLM ID: 8506292 Publication Model: Print Cited Medium: Print ISSN: 8756-7938 (Print) Linking ISSN: 15206033 NLM ISO Abbreviation: Biotechnol Prog Subsets: MEDLINE

مواضيع طبية MeSH: Bioreactors*/*microbiology, Protein Engineering/*methods , Protein Splicing/*physiology , Proto-Oncogene Proteins c-myb/*biosynthesis , Proto-Oncogene Proteins c-myb/*isolation & purification , Recombinant Fusion Proteins/*biosynthesis , Recombinant Fusion Proteins/*isolation & purification, Cell Count ; Cell Culture Techniques/methods ; Escherichia coli/genetics ; Escherichia coli/growth & development ; Escherichia coli/metabolism ; Gene Expression Regulation, Bacterial ; Industrial Microbiology/methods ; Proto-Oncogene Proteins c-myb/genetics ; Recombinant Fusion Proteins/genetics