المؤلفون: Ni Liu, Dan Pi, Chun-Mei Liu

المصدر: Journal of Hainan Medical University, Vol 24, Iss 01, Pp 5-8 (2018)

مصطلحات موضوعية: GRP78, endocrine system diseases, 409000. Fund Project: Science and Technology Project of Qianjiang District No: 2016056. 1. Introduction Ovarian cancer is one of the most common malignant tumors in the female reproductive system. Surgical resection and chemotherapy are the main treatments. Because ovarian cancer cells are more sensitive to chemotherapy drugs, Proliferation, DMEM, RIPA lysate was purchased in Shanghai Beyotime Company, lcsh:Medicine, Invasion, Chongqing, Ovarian cancer, especially platinum drugs, and enzyme-linked immunosorbent assay kits were purchased from Shanghai Westang Biotechnology Company. Objective: To study the effect of metformin combined with cisplatin on the expression of genes related to ovarian cancer cell growth and invasion in vitro. Methods: SKOV3 ovarian cancer cell lines were cultured and randomly divided into the control group treated with serumfree DMEM, the platinum-based intravenous chemotherapy is widely used in the treatment of advanced ovarian cancer[1, CyclinD1, the MET group treated with serum-free DMEM containing 10 mmol/L metformin and the DDP+MET group treated with serum-free DMEM containing 10 μmol/L cisplatin and 10 mmol/L metformin. Results: 24 h after treatment, some patients with ovarian cancer can develop drug resistance during chemotherapy, lcsh:R, TBX2, it has been confirmed in recent years that it has anti-tumor effect and chemotherapy drug sensitization effect, the growth and invasion of cancer cells involve the changes in the expression of multiple proliferationrelated genes and invasion-related genes. In the following studies, Metformin, the DDP group treated with serum-free DMEM containing 10 μmol/L cisplatin, Department of Obstetrics and Gynecology, which will affect the efficacy of chemotherapy[3]. Metformin is a kind of insulin sensitizer for diabetes treatment, Ki-67, Chongqing Qianjiang National Hospital, and its combination with a variety of chemotherapy drugs can enhance the killing effect of chemotherapy drugs on tumor cells[4]. In the progression of the course of ovarian cancer, Cisplatin, we specifically analyzed the effects of metformin combined with cisplatin on the expression of genes associated with ovarian cancer cell growth and invasion in vitro. 2. Experimental materials and methods 2.1 Experimental materials Ovarian cancer SKOV3 cell lines were bought in the cell bank of Chinese Academy of Sciences, fetal bovine serum and 0.125% trypsin for cell culture were purchased in Hyclone Company, cisplatin and metformin were bought in the Sigma Company

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