المؤلفون: Yeh, C.-F., Tai, W., Lin, C.-H., Juang, D. S., Wu, C.-C., Chen, Y.-W., Hsu, C.-H.

المصدر: IEEE Transactions on NanoBioscience IEEE Trans.on Nanobioscience NanoBioscience, IEEE Transactions on. 14(8):835-840 Dec, 2015

المؤلفون: Shi, Yaqi, Yang, Zonglin, Xing, Lei, Zhang, Xuzhi, Li, Xianguo, Zhang, Dahai^Aff1

المصدر: World Journal of Microbiology and Biotechnology. 37(8)

المؤلفون: Monthira Monthatong

مصطلحات موضوعية: Male-specific marker, RPA, SRY1.Introduction Biological evidence can be used to determine the gender of victims or suspects in forensic cases, including murder, rape and missing persons [1]. Commonly, the morphological and anatomical characteristics of sex organs are used to determine the gender of an unidentified body. Currently, the polymerase chain reaction (PCR) or multiplex PCR techniques are routinely used in the molecular biological process for determining the human gender of organic traces [2]. In forensic study, gender determination was commonly performed using PCR with both the amelogenin (AMEL) and sex-determining region Y (SRY) genes [3]. However, PCR-based assay still has some disadvantages, such as being time-consuming, depending on complicated thermal cycling machines, having a high energy cost, and requiring precise laboratory facilities and highly skilled operators, which restrict the application of PCR in small or unspecialized laboratories and in resource-limited settings. These limitations in PCR-based methods have urged the development of a new molecular biological technique known as isothermal Deoxyribonucleic acid (DNA) amplification that has been extensively reviewed [4-9]. One isothermal DNA amplification technique is called recombinase polymerase amplification (RPA) [10]. RPA is a highly sensitive and selective isothermal amplification technique, performed at a single temperature in the range 37-42?C with minimal sample preparation and is capable of amplifying as low as 1-10 targeted DNA copies in less than 20 min without the need for an initial denaturation step or the use of multiple primers. RPA uses three proteins, including recombinase, single-stranded DNA binding protein (SSB) and strand-displacing polymerase, to amplify double-stranded Deoxyribonucleic acid (dsDNA) in a similar way to the PCR method [11,12]. In the RPA reaction, the recombinases combine with the primers to form recombinase-primer complexes that subsequently scan the double-stranded DNA template for the homologous sequence. The strand displacement reaction is initiated to synthesize new DNA sequences when the primers hybridize to their complementary strand. The displaced template strand is stabilized by SSB and the recombinase dissociates and allows elongation of primers by strand-displacing DNA polymerase. The process generates a complete copy of the

URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_________::284e67428443c19bd08b5202ee87a203

المؤلفون: Nakano, Kazuhiko^Aff00091, Nakagawa, Ichiro^Aff00092, Alaluusua, Satu^Aff00093, Ooshima, Takashi^Aff00091

المساهمون: de Filippis, Ivano, editor^AffID1, McKee, Marian L., editor^AffID2

المصدر: Molecular Typing in Bacterial Infections. :127-147

المؤلفون: Freeman, Ray^Aff2, Kupče, Eriks^Aff3

المساهمون: Arrondo, José Luis R., editor^Aff1, Alonso, Alicia, editor^Aff1

المصدر: Advanced Techniques in Biophysics. 10:129-145

المؤلفون: Flach, Carol R.^Aff2, Cai, Peng^Aff2, Mendelsohn, Richard^Aff2

المساهمون: Arrondo, José Luis R., editor^Aff1, Alonso, Alicia, editor^Aff1

المصدر: Advanced Techniques in Biophysics. 10:49-71

المؤلفون: Ibarra-Molero, Beatriz^Aff2, Sanchez-Ruiz, Jose M.^Aff2

المساهمون: Arrondo, José Luis R., editor^Aff1, Alonso, Alicia, editor^Aff1

المصدر: Advanced Techniques in Biophysics. 10:27-48

المؤلفون: Ziegler, A.^Aff6, Hartmann, O.^Aff6, König, I. R.^Aff6, Schäfer, H.^Aff6

المساهمون: Bock, H.-H., editor^Aff1, Gaul, W., editor^Aff2, Schader, M., editor^Aff3, Schwaiger, Manfred, editor^Aff4, Opitz, Otto, editor^Aff5

المصدر: Exploratory Data Analysis in Empirical Research : Proceedings of the 25th Annual Conference of the Gesellschaft für Klassifikation e.V., University of Munich, March 14–16, 2001. :401-409

المؤلفون: Sourdive, David^Aff4, Garrigue, Henri^Aff4

المساهمون: Dando, Malcolm R., editor^Aff1, Klement, Cyril, editor^Aff2, Negut, Marian, editor^Aff3, Pearson, Graham S., editor^Aff1

المصدر: Maximizing the Security and Development Benefits from the Biological and Toxin Weapons Convention. 36:143-148

المؤلفون: Dick, Michael W.^Aff2

المساهمون: Dick, Michael W.^Aff1

المصدر: Straminipilous Fungi : Systematics of the Peronosporomycetes Including Accounts of the Marine Straminipilous Protists, the Plasmodiophorids and Similar Organisms. :101-121