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المؤلفون: Arnaud Poterszman, Didier Busso, Timothy J. Richmond, Christophe Romier, Christoph Bieniossek, Lakshmi Sumitra Vijayachandran, Christiane Schaffitzel, Imre Berger, Cristina Viola, Frederic Garzoni, Simon Trowitzsch, Maxime Chaillet
المصدر: Journal of Structural Biology
Journal of structural biologyمصطلحات موضوعية: Cell, Polyprotein, NMR, nuclear magnetic resonance (spectroscopy), Protein Engineering, Recombineering, ACEMBL, Automation, 0302 clinical medicine, polh, polyhedrin baculoviral very late promoter, Baculovirus, Cloning, Molecular, MOI, multiplicity of infection, Mammalian host, Cells, Cultured, Genetics, Insect cells, 0303 health sciences, dpa, day of proliferation arrest, HT, high throughput, Sf9, Sf21, Spodoptera frugiperda cell lines 9 or 21, respectively, E. coli, Escherichia coli, Academies and Institutes, Robotics, EGFP, enhanced green fluorescent protein, TEV, tobacco etch virus, resp. a protease (N1A) from this virus, kb, kilo base, Recombinant Proteins, Europe, medicine.anatomical_structure, SDS–PAGE, sodium dodecylsulfate–polyacryamide gel electrophoresis, MultiBac, BEVS, Ori, origin of replication, R6Kγ, bacteriophage R6Kγ, Structural biology, Baculoviridae, Polyproteins, Green Fluorescent Proteins, High-throughput, Computational biology, Biology, Spodoptera, FRET, fluorescence resonance energy transfer, Article, 03 medical and health sciences, tcs, TEV protease cleavage site, ORF, open reading frame, kDa, kilo dalton, medicine, Escherichia coli, Animals, Humans, BEVS, baculovirus expression vector system, CFP, cyan florescent protein, pET-MCN, 030304 developmental biology, Cloning, Automation, Laboratory, EM, electron microscopy, Protein engineering, CMV, cytomegalovirus, YFP, yellow fluorescent protein, Luminescent Proteins, Förster resonance energy transfer, dsRed, red fluorescent protein, Multiprotein Complexes, Heterologous expression, SLIC, sequence and ligation independent cloning, p10, p10 baculoviral late promoter, 030217 neurology & neurosurgery
