دورية أكاديمية
Activation of the mouse Ig germline epsilon promoter by IL-4 is dependent on AP-1 transcription factors.
| العنوان: | Activation of the mouse Ig germline epsilon promoter by IL-4 is dependent on AP-1 transcription factors. |
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| المؤلفون: | Shen CH; Department of Molecular Genetics and Microbiology, University of Massachusetts Medical School, Worcester, MA 01655, USA., Stavnezer J |
| المصدر: | Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2001 Jan 01; Vol. 166 (1), pp. 411-23. |
| نوع المنشور: | Journal Article; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Association of Immunologists Country of Publication: United States NLM ID: 2985117R Publication Model: Print Cited Medium: Print ISSN: 0022-1767 (Print) Linking ISSN: 00221767 NLM ISO Abbreviation: J Immunol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Bethesda, MD : American Association of Immunologists Original Publication: Baltimore : Williams & Wilkins, c1950- |
| مواضيع طبية MeSH: | Gene Expression Regulation/*immunology , Immunoglobulin epsilon-Chains/*genetics , Immunoglobulin epsilon-Chains/*metabolism , Interleukin-4/*physiology , Promoter Regions, Genetic/*immunology , Transcription Factor AP-1/*physiology, Adjuvants, Immunologic/physiology ; Animals ; B-Lymphocytes/immunology ; B-Lymphocytes/metabolism ; Base Sequence ; CCAAT-Enhancer-Binding Proteins/biosynthesis ; CCAAT-Enhancer-Binding Proteins/genetics ; CCAAT-Enhancer-Binding Proteins/physiology ; CD40 Ligand/physiology ; Cell Line ; Cells, Cultured ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/metabolism ; Genes, Reporter/immunology ; Half-Life ; Humans ; Immunoglobulin epsilon-Chains/biosynthesis ; Kinetics ; Lymphocyte Activation/immunology ; Mice ; Mice, Inbred BALB C ; Molecular Sequence Data ; Multigene Family/immunology ; Plasmids/genetics ; Plasmids/metabolism ; Proto-Oncogene Proteins c-fos/physiology ; Proto-Oncogene Proteins c-jun/physiology ; STAT6 Transcription Factor ; Signal Transduction/genetics ; Signal Transduction/immunology ; Spleen/cytology ; Trans-Activators/antagonists & inhibitors ; Trans-Activators/physiology ; Transcription Factor AP-1/antagonists & inhibitors ; Transcription Factor AP-1/biosynthesis ; Transcription Factor AP-1/metabolism ; Transfection ; Tumor Cells, Cultured |
| مستخلص: | Induction of germline (GL) epsilon transcripts, an essential step preceding Ig isotype switching to IgE, requires activation of transcription factors by IL-4 and a B cell activator, e.g., CD40 ligand or LPS. We demonstrate that AP-1 (Fos and Jun), induced transiently by CD40 ligand or LPS, binds a DNA element in the mouse GL epsilon promoter. AP-1 synergizes with Stat6 to activate both the intact GL epsilon promoter and a minimal heterologous promoter driven by the AP-1 and Stat6 sites of the mouse GL epsilon promoter. By contrast, C/EBP beta, which trans-activates the human GL epsilon promoter, inhibits IL-4 induction of the mouse promoter, probably by attenuating the synergistic interaction between AP-1 and Stat6. Furthermore, AP-1 does not trans-activate the human GL epsilon promoter. Thus, induction of GL epsilon transcripts in mice and humans may be regulated differently. In addition, although mouse GL epsilon transcripts have a half-life of approximately 100 min, the RNA level continues to increase for up to 24 h, and the promoter appears to be active for at least 2 days after B cell activation. Altogether, these data suggest that induction of AP-1 activity, although transient, is required for activation of the mouse GL epsilon promoter by IL-4-induced Stat6. |
| معلومات مُعتمدة: | AI42108 United States AI NIAID NIH HHS |
| المشرفين على المادة: | 0 (Adjuvants, Immunologic) 0 (CCAAT-Enhancer-Binding Proteins) 0 (DNA-Binding Proteins) 0 (Immunoglobulin epsilon-Chains) 0 (Proto-Oncogene Proteins c-fos) 0 (Proto-Oncogene Proteins c-jun) 0 (STAT6 Transcription Factor) 0 (STAT6 protein, human) 0 (Stat6 protein, mouse) 0 (Trans-Activators) 0 (Transcription Factor AP-1) 147205-72-9 (CD40 Ligand) 207137-56-2 (Interleukin-4) |
| تواريخ الأحداث: | Date Created: 20001221 Date Completed: 20010208 Latest Revision: 20190515 |
| رمز التحديث: | 20240829 |
| DOI: | 10.4049/jimmunol.166.1.411 |
| PMID: | 11123319 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0022-1767 |
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| DOI: | 10.4049/jimmunol.166.1.411 |