دورية أكاديمية
أعرض في EDS

Effects of genotypically different strains of Helicobacter pylori on human microvascular endothelial cells in vitro.

التفاصيل البيبلوغرافية
العنوان: Effects of genotypically different strains of Helicobacter pylori on human microvascular endothelial cells in vitro.
المؤلفون: Kalia N; Department of Surgical and Anaesthetic Sciences, Royal Hallamshire Hospital, University of Sheffield, UK., Jones C, Bardhan DK, Reed MW, Atherton JC, Brown NJ
المصدر: Digestive diseases and sciences [Dig Dis Sci] 2001 Jan; Vol. 46 (1), pp. 54-61.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Springer Science + Business Media Country of Publication: United States NLM ID: 7902782 Publication Model: Print Cited Medium: Print ISSN: 0163-2116 (Print) Linking ISSN: 01632116 NLM ISO Abbreviation: Dig Dis Sci Subsets: MEDLINE
أسماء مطبوعة: Publication: 2005- : New York, NY : Springer Science + Business Media
Original Publication: New York, Plenum Pub. Corp.
مواضيع طبية MeSH: Endothelium, Vascular/*pathology , Helicobacter pylori/*genetics , Stomach/*blood supply, Apoptosis ; Benzimidazoles ; Cell Survival ; Cells, Cultured ; Endothelium, Vascular/microbiology ; Genotype ; Humans ; Microcirculation/pathology ; Necrosis ; Propidium
مستخلص: Helicobacter pylori induces a number of disturbances in rodent gastric microcirculation in vivo. These events may result from direct necrotic or apoptotic damage to endothelial cells. This study therefore aimed to investigate the effects of genotypically different H. pylori strains on microvascular endothelial cell (MVEC) viability in vitro. Four H. pylori extracts were prepared from strains with different cagA or vacA status. MVECs were plated into 96-well plates and coincubated with 50 microl of extract or vehicle for 24, 48, 72, or 96 hr. An MPP assay quantified overall MVEC viability. The dual labeling of MVECs with propidium iodide and Hoechst 33342 distinguished between necrotic and apoptotic cell death, respectively, and allowed total number of viable cells to be determined. All strains of H. pylori decreased cell viability after 72 and 96 hr. Neither necrosis or apoptosis was observed. Counting total number of viable cells revealed decreased cell proliferation with all strains when compared to controls, again reaching significance at 72 and 96 hr. In conclusion, both the MTT assay and the diret cell counting technique demonstrated that all H. pylori strains induced cytostatic but not cytotoxic effects on MVECs. This suggests that microcirculatory disturbances observed in vivo may not be the result of direct endothelial cell damage. However, inhibition of angiogenesis may explain why ulcer healing is delayed in H. pylori-infected patients.
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المشرفين على المادة: 0 (Benzimidazoles)
36015-30-2 (Propidium)
P976261J69 (bisbenzimide ethoxide trihydrochloride)
تواريخ الأحداث: Date Created: 20010329 Date Completed: 20010419 Latest Revision: 20190721
رمز التحديث: 20240829
DOI: 10.1023/a:1005653607433
PMID: 11270794
قاعدة البيانات: MEDLINE
الوصف
تدمد:0163-2116
DOI:10.1023/a:1005653607433