دورية أكاديمية
Specific depletion of human anti-adenovirus antibodies facilitates transduction in an in vivo model for systemic gene therapy.
| العنوان: | Specific depletion of human anti-adenovirus antibodies facilitates transduction in an in vivo model for systemic gene therapy. |
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| المؤلفون: | Rahman A; Department of Molecular Biology, Canji, Inc., 3525 John Hopkins Court, San Diego, CA 92121, USA., Tsai V, Goudreau A, Shinoda JY, Wen SF, Ramachandra M, Ralston R, Maneval D, LaFace D, Shabram P |
| المصدر: | Molecular therapy : the journal of the American Society of Gene Therapy [Mol Ther] 2001 May; Vol. 3 (5 Pt 1), pp. 768-78. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 100890581 Publication Model: Print Cited Medium: Print ISSN: 1525-0016 (Print) Linking ISSN: 15250016 NLM ISO Abbreviation: Mol Ther Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2017- : Cambridge, MA : Cell Press Original Publication: San Diego, CA : Academic Press, 2000- |
| مواضيع طبية MeSH: | Gene Transfer Techniques* , Transduction, Genetic*, Adenoviridae/*genetics , Genetic Therapy/*methods, Adenoviridae/immunology ; Animals ; Antibodies/metabolism ; Green Fluorescent Proteins ; Humans ; Immunization, Passive ; Luminescent Proteins/metabolism ; Mice ; Mice, Inbred BALB C ; Mice, SCID ; Protein Biosynthesis ; Reverse Transcriptase Polymerase Chain Reaction ; beta-Galactosidase/metabolism |
| مستخلص: | Recombinant adenoviral (rAd) vectors are capable of mediating high-efficiency gene transfer in vivo. Under conditions requiring systemic administration, however, the use of rAd vectors can be problematic due to the presence of circulating anti-adenovirus antibodies developed either through natural infection or during the course of treatment. We developed a passive immunization model in SCID/Beige mice to assess the effect of human and mouse anti-adenovirus antibodies on systemic administration of a rAd vector expressing beta-galactosidase (rAd-betagal). In this model, the in vitro neutralizing activity of human or mouse antibodies used for passive immunization correlated well with inhibition of transduction of the liver following i.v. administration of rAd-betagal. Depletion of antibodies to individual adenovirus structural proteins (hexon, penton, fiber) by affinity chromatography demonstrated that antibodies to each of the three virion components contributed to neutralization of infectivity in vitro and to inhibition of transduction in vivo. Depletion of antibodies against all three structural proteins from human or mouse immune serum prior to passive immunization restored in vivo transduction activity to levels comparable to those obtained with nonimmune serum. Our data suggest that depletion of both murine and human anti-adenoviral antibodies can restore transduction in vivo during systemic rAd gene therapy in hosts previously exposed to adenovirus. |
| المشرفين على المادة: | 0 (Antibodies) 0 (Luminescent Proteins) 147336-22-9 (Green Fluorescent Proteins) EC 3.2.1.23 (beta-Galactosidase) |
| تواريخ الأحداث: | Date Created: 20010518 Date Completed: 20010726 Latest Revision: 20121115 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1006/mthe.2001.0316 |
| PMID: | 11356081 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1525-0016 |
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| DOI: | 10.1006/mthe.2001.0316 |