دورية أكاديمية
Plasmodium gallinaceum: ookinete formation and proteolytic enzyme dynamics in highly refractory Aedes aegypti populations.
| العنوان: | Plasmodium gallinaceum: ookinete formation and proteolytic enzyme dynamics in highly refractory Aedes aegypti populations. |
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| المؤلفون: | Kaplan RA; Department of Biological Sciences, State University of New York, Buffalo, New York 14260, USA., Zwiers SH, Yan G |
| المصدر: | Experimental parasitology [Exp Parasitol] 2001 Jul; Vol. 98 (3), pp. 115-22. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 0370713 Publication Model: Print Cited Medium: Print ISSN: 0014-4894 (Print) Linking ISSN: 00144894 NLM ISO Abbreviation: Exp Parasitol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Orlando, FL : Academic Press Original Publication: New York. |
| مواضيع طبية MeSH: | Aedes/*enzymology , Aedes/*parasitology , Aminopeptidases/*metabolism , Insect Vectors/*enzymology , Insect Vectors/*parasitology , Plasmodium gallinaceum/*growth & development , Trypsin/*metabolism, Aedes/immunology ; Animals ; Blood Proteins/metabolism ; Chickens ; Female ; Insect Vectors/immunology |
| مستخلص: | Despite significant progress in the identification of the genetic basis of the refractory phenotype, little is known about the physiological mechanism of refractoriness. This study therefore examined the physiological basis of mosquito refractoriness in the Aedes aegypti/P. gallinaceum system, in which a selected refractory strain does not permit Plasmodium oocyst formation. We examined the kinetics of two major proteolytic enzymes involved in blood meal digestion and the dynamics of ookinete formation for two refractory populations (strains Moyo-R and Formosus) and one susceptible population (strain Red). Healthy ookinetes were observed in both the susceptible and the refractory populations, although the susceptible population generally exhibited higher enzymatic activity for trypsin and aminopeptidase than the refractory populations. Parasite numbers in the susceptible Red population showed a 4- to 7-fold decrease in abundance during the transition from the ookinete stage to the oocyst stage, far less than the refractory populations (30- to 92-fold reduction). Due to its smaller body size, Moyo-R individuals generally ingest a smaller blood meal and thus intake fewer gametocytes than Red individuals. Thus, the possibility that refractoriness in the Moyo-R population results from fewer gametocytes being ingested is examined. We found that the Red population remained highly susceptible and the Moyo-R population stayed refractory when those individuals with similar blood meal size were compared. We conclude that failure of oocyst development in the refractory mosquitoes is not due to ookinete damage by proteolytic enzymes or to fewer gametocytes being ingested, but rather is due to a midgut barrier or to some other mechanism. (Copyright 2001 Academic Press.) |
| معلومات مُعتمدة: | U19 AI45511 United States AI NIAID NIH HHS |
| المشرفين على المادة: | 0 (Blood Proteins) EC 3.4.11.- (Aminopeptidases) EC 3.4.21.4 (Trypsin) |
| تواريخ الأحداث: | Date Created: 20010831 Date Completed: 20010927 Latest Revision: 20071114 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1006/expr.2001.4623 |
| PMID: | 11527434 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0014-4894 |
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| DOI: | 10.1006/expr.2001.4623 |