دورية أكاديمية
Cytokine-stimulated T cells induce macrophage IL-10 production dependent on phosphatidylinositol 3-kinase and p70S6K: implications for rheumatoid arthritis.
| العنوان: | Cytokine-stimulated T cells induce macrophage IL-10 production dependent on phosphatidylinositol 3-kinase and p70S6K: implications for rheumatoid arthritis. |
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| المؤلفون: | Foey A; Kennedy Institute of Rheumatology Division, Imperial College School of Medicine, Hammersmith, London, W6 8LH, UK. a.foey@ic.ac.uk, Green P, Foxwell B, Feldmann M, Brennan F |
| المصدر: | Arthritis research [Arthritis Res] 2002; Vol. 4 (1), pp. 64-70. Date of Electronic Publication: 2001 Oct 10. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: BioMed Central Ltd Country of Publication: England NLM ID: 100913255 Publication Model: Print-Electronic Cited Medium: Print ISSN: 1465-9905 (Print) Linking ISSN: 14659905 NLM ISO Abbreviation: Arthritis Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London, UK : BioMed Central Ltd Original Publication: London, UK : Current Science, c1999-c2002. |
| مواضيع طبية MeSH: | Arthritis, Rheumatoid/*enzymology , Interleukin-10/*biosynthesis , Macrophages/*enzymology , Phosphatidylinositol 3-Kinases/*metabolism , Ribosomal Protein S6 Kinases/*metabolism , T-Lymphocytes/*enzymology, Androstadienes/pharmacology ; Arthritis, Rheumatoid/pathology ; Cells, Cultured ; Chromones/pharmacology ; Coculture Techniques ; Cytokines/pharmacology ; Enzyme Inhibitors ; Humans ; Macrophages/drug effects ; Morpholines/pharmacology ; Phosphoinositide-3 Kinase Inhibitors ; Ribosomal Protein S6 Kinases/antagonists & inhibitors ; Signal Transduction ; Sirolimus/pharmacology ; Synovial Membrane/metabolism ; Synovial Membrane/pathology ; T-Lymphocytes/drug effects ; Wortmannin |
| مستخلص: | IL-10 is an anti-inflammatory cytokine produced in the joint in rheumatoid arthritis by macrophages and infiltrating blood lymphocytes. Regulation of its expression is poorly understood, but previous findings have suggested that physical interactions with T cells may play a role. This report investigates signalling mechanisms involved in the production of macrophage IL-10 upon interaction with fixed, cytokine-stimulated T cells (Tck). Elutriated monocytes were differentiated to macrophages by macrophage-colony-stimulating factor (M-CSF) and co-cultured with fixed T cells chronically stimulated in a cytokine cocktail of IL-2/IL-6/tumour necrosis factor (TNF)-alpha in the presence or absence of wortmannin and LY294002, inhibitors of phosphatidylinositol 3-kinase (PI3K), or of rapamycin, an inhibitor of p70 S6-kinase (p70S6K). Spontaneous IL-10 production by rheumatoid arthritis synovial-membrane mononuclear cells (RA-SMCs) and co-cultures of rheumatoid arthritis T cells (RA-Ts) and macrophages was also assessed. RA-T and Tck induction of macrophage IL-10 production was suppressed by cell separation and inhibition of PI3K and p70S6K. PI3K involvement was also shown by phosphorylation of the downstream effector protein kinase B. Spontaneous IL-10 production by RA-SMCs was also inhibited by LY294002 and depletion of the nonadherent (T-cell-enriched) fraction of the cell population. IL-10 production in RA-SMCs and M-CSF-primed macrophages, activated by interaction with Tck, is PI3K- and p70S6K-dependent. |
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| المشرفين على المادة: | 0 (Androstadienes) 0 (Chromones) 0 (Cytokines) 0 (Enzyme Inhibitors) 0 (Morpholines) 0 (Phosphoinositide-3 Kinase Inhibitors) 130068-27-8 (Interleukin-10) 31M2U1DVID (2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one) EC 2.7.11.1 (Ribosomal Protein S6 Kinases) W36ZG6FT64 (Sirolimus) XVA4O219QW (Wortmannin) |
| تواريخ الأحداث: | Date Created: 20020307 Date Completed: 20020418 Latest Revision: 20191210 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC64854 |
| DOI: | 10.1186/ar385 |
| PMID: | 11879539 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1465-9905 |
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| DOI: | 10.1186/ar385 |