دورية أكاديمية
Activation of Kupffer cells inhibits tumor growth in a murine model system.
| العنوان: | Activation of Kupffer cells inhibits tumor growth in a murine model system. |
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| المؤلفون: | Chen GG; Department of Surgery, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong. gchen@cuhk.edu.hk, Lau WY, Lai PB, Chun YS, Chak EC, Leung BC, Lam IK, Lee JF, Chui AK |
| المصدر: | International journal of cancer [Int J Cancer] 2002 Jun 10; Vol. 99 (5), pp. 713-20. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-Liss Country of Publication: United States NLM ID: 0042124 Publication Model: Print Cited Medium: Print ISSN: 0020-7136 (Print) Linking ISSN: 00207136 NLM ISO Abbreviation: Int J Cancer Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 1995- : New York, NY : Wiley-Liss Original Publication: 1966-1984 : Genève : International Union Against Cancer |
| مواضيع طبية MeSH: | Kupffer Cells/*physiology , Neoplasms, Experimental/*prevention & control, Animals ; Apoptosis ; Blotting, Western ; Caspase 8 ; Caspase 9 ; Caspases/metabolism ; DNA Damage ; Glioma/pathology ; Glioma/prevention & control ; Immunohistochemistry ; Interferon-gamma/analysis ; Mice ; Mice, Inbred BALB C ; Mice, Nude ; Neoplasm Transplantation ; Neoplasms, Experimental/pathology ; Nitric Oxide/analysis ; Proto-Oncogene Proteins c-bcl-2/analysis ; Rats ; Tumor Necrosis Factor-alpha/analysis |
| مستخلص: | Kupffer cells, a liver organ-specific macrophage, play an important role in preventing the development of malignant tumors. The mechanism responsible for their tumoricidal activities is not completely known. In our study, we established in vivo models involving a rat malignant cell line, rat Kupffer cells and tumor implantation in nude mice. A series of relevant in vitro experiments were also carried out to determine possible pathways. LPS-activated Kupffer cells produced significant amounts of NO, TNFalpha and IFNgamma. Malignant cells treated with either Kupffer cells or culture supernatant of the activated Kupffer cells had an increase in caspase-8 activity. Implanted tumors originated from malignant cells treated with either Kupffer cells or culture supernatant of the activated Kupffer cells grew much smaller than those from malignant cells without treatment or treated with control supernatants. The alteration of anti-apoptotic Bcl-2 was inversely associated with the change of pro-apoptotic caspase-8 and their levels in the tumor tissues matched the size of the tumors and treatments they received. It appeared that the above changes resulted in an increase in cellular DNA damage and apoptosis seen in malignant cells. Therefore, Kupffer cells execute their anti-tumor effect via increasing the production of NO, TNFalpha and IFNgamma and these cytotoxic molecules inhibit the growth of tumor by damaging cellular DNA and inducing apoptosis that was featured by downregulation of Bcl-2 but upregulation of caspase-8. (Copyright 2002 Wiley-Liss, Inc.) |
| المشرفين على المادة: | 0 (Proto-Oncogene Proteins c-bcl-2) 0 (Tumor Necrosis Factor-alpha) 31C4KY9ESH (Nitric Oxide) 82115-62-6 (Interferon-gamma) EC 3.4.22.- (Casp8 protein, mouse) EC 3.4.22.- (Casp8 protein, rat) EC 3.4.22.- (Casp9 protein, mouse) EC 3.4.22.- (Casp9 protein, rat) EC 3.4.22.- (Caspase 8) EC 3.4.22.- (Caspase 9) EC 3.4.22.- (Caspases) |
| تواريخ الأحداث: | Date Created: 20020713 Date Completed: 20020729 Latest Revision: 20201226 |
| رمز التحديث: | 20240829 |
| DOI: | 10.1002/ijc.10412 |
| PMID: | 12115505 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0020-7136 |
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| DOI: | 10.1002/ijc.10412 |