دورية أكاديمية
Role of the actin cytoskeleton in store-mediated calcium entry in glioma C6 cells.
| العنوان: | Role of the actin cytoskeleton in store-mediated calcium entry in glioma C6 cells. |
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| المؤلفون: | Sabała P; Laboratory of Signal Transduction, Department of Molecular and Cellular Neurobiology, Nencki Institute of Experimental Biology, Polish Academy of Sciences, 3 Pasteur Str., Warsaw, Poland. sabala@nencki.gov.pl, Targos B, Caravelli A, Czajkowski R, Lim D, Gragnaniello G, Santella L, Barańska J |
| المصدر: | Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2002 Aug 16; Vol. 296 (2), pp. 484-91. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0372516 Publication Model: Print Cited Medium: Print ISSN: 0006-291X (Print) Linking ISSN: 0006291X NLM ISO Abbreviation: Biochem Biophys Res Commun Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2002- >: San Diego, CA : Elsevier Original Publication: New York, Academic Press. |
| مواضيع طبية MeSH: | Actins/*metabolism , Calcium/*metabolism , Calcium Signaling/*physiology , Cytoskeleton/*metabolism, Adenosine Diphosphate/metabolism ; Animals ; Bridged Bicyclo Compounds, Heterocyclic/pharmacology ; Chelating Agents/metabolism ; Cytochalasin D/pharmacology ; Enzyme Inhibitors/pharmacology ; Fluorescent Dyes/metabolism ; Fura-2/metabolism ; Glioma ; Microscopy, Confocal ; Nucleic Acid Synthesis Inhibitors/pharmacology ; Rats ; Thapsigargin/pharmacology ; Thiazoles/pharmacology ; Thiazolidines ; Tumor Cells, Cultured ; Uridine Triphosphate/metabolism |
| مستخلص: | The effects of actin cytoskeleton disruption by cytochalasin D and latrunculin A on Ca2+ signals evoked by ADP, UTP or thapsigargin were investigated in glioma C6 cells. Despite the profound alterations of the actin cytoskeleton architecture and cell morphology, ADP and UTP still produced cytosolic calcium elevation in this cell line. However, calcium mobilization from internal stores and Ca2+ influx through store-operated Ca2+ channels induced by ADP and UTP were strongly reduced. Cytochalasin D and latrunculin A also diminished extracellular Ca2+ influx in unstimulated glioma C6 cells previously incubated in Ca2+ free buffer. In contrast, the disruption of the actin cytoskeleton had no effect on thapsigargin-induced Ca2+ influx in this cell line. Both agonist- and thapsigargin-generated Ca2+ entry was significantly decreased by the blocker of store-operated Ca2+ channels, 2-aminoethoxydiphenylborate. The data reveal that two agonists and thapsigargin activate store-operated Ca2+ channels but the mechanism of activation seems to be different. While the agonists evoke a store-mediated Ca2+ entry that is dependent on the actin cytoskeleton, thapsigargin apparently activates an additional mechanism, which is independent of the disruption of the cytoskeleton. |
| المشرفين على المادة: | 0 (Actins) 0 (Bridged Bicyclo Compounds, Heterocyclic) 0 (Chelating Agents) 0 (Enzyme Inhibitors) 0 (Fluorescent Dyes) 0 (Nucleic Acid Synthesis Inhibitors) 0 (Thiazoles) 0 (Thiazolidines) 22144-77-0 (Cytochalasin D) 61D2G4IYVH (Adenosine Diphosphate) 67526-95-8 (Thapsigargin) SRQ9WWM084 (latrunculin A) SY7Q814VUP (Calcium) TSN3DL106G (Fura-2) UT0S826Z60 (Uridine Triphosphate) |
| تواريخ الأحداث: | Date Created: 20020807 Date Completed: 20020930 Latest Revision: 20190612 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/s0006-291x(02)00893-8 |
| PMID: | 12163045 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0006-291X |
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| DOI: | 10.1016/s0006-291x(02)00893-8 |