دورية أكاديمية
Functional leukemia-associated antigen-specific memory CD8+ T cells exist in healthy individuals and in patients with chronic myelogenous leukemia before and after stem cell transplantation.
| العنوان: | Functional leukemia-associated antigen-specific memory CD8+ T cells exist in healthy individuals and in patients with chronic myelogenous leukemia before and after stem cell transplantation. |
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| المؤلفون: | Rezvani K; National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA., Grube M, Brenchley JM, Sconocchia G, Fujiwara H, Price DA, Gostick E, Yamada K, Melenhorst J, Childs R, Hensel N, Douek DC, Barrett AJ |
| المصدر: | Blood [Blood] 2003 Oct 15; Vol. 102 (8), pp. 2892-900. Date of Electronic Publication: 2003 Jun 26. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 7603509 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0006-4971 (Print) Linking ISSN: 00064971 NLM ISO Abbreviation: Blood Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2021- : [New York] : Elsevier Original Publication: New York, Grune & Stratton [etc.] |
| مواضيع طبية MeSH: | CD8-Positive T-Lymphocytes/*metabolism , HLA-A Antigens/*blood , Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*therapy, CD3 Complex/biosynthesis ; CD8 Antigens/biosynthesis ; Cell Line ; DNA, Complementary/metabolism ; Flow Cytometry ; HLA-A2 Antigen ; Humans ; Immunologic Memory ; Leukemia/metabolism ; Peptides/chemistry ; Phenotype ; Polymerase Chain Reaction ; RNA/metabolism ; Stem Cell Transplantation ; T-Lymphocytes/metabolism ; Time Factors |
| مستخلص: | Antigens implicated in the graft-versus-leukemia (GVL) effect in chronic myeloid leukemia (CML) include WT1, PR1, and BCR-ABL. To detect very low frequencies of these antigen-specific CD8+ T cells, we used quantitative polymerase chain reaction (qPCR) to measure interferon-gamma (IFN-gamma) mRNA production by peptide-pulsed CD8+ T cells from HLA-A*0201+ healthy volunteers and from patients with CML before and after allogeneic stem cell transplantation (SCT). Parallel assays using cytomegalovirus (CMV) pp65 tetramers demonstrated the IFN-gamma copy number to be linearly related to the frequency of tetramer-binding T cells, sensitive to frequencies of 1 responding CD8+ T cell/100 000 CD8+ T cells. Responses to WT1 and PR1 but not BCR-ABL were detected in 10 of 18 healthy donors. Responses to WT1, PR1, or BCR-ABL were observed in 9 of 14 patients with CML before SCT and 5 of 6 after SCT, often to multiple epitopes. Responses were higher in patients with CML compared with healthy donors and highest after SCT. These antigen-specific CD8+ T cells comprised central memory (CD45RO+CD27+CD57-) and effector memory (CD45RO-CD27-CD57+) T cells. In conclusion, leukemia-reactive CD8+ T cells derive from memory T cells and occur at low frequencies in healthy individuals and at higher frequencies in patients with CML. The increased response in patients after SCT suggests a quantitative explanation for the greater effect of allogeneic SCT. |
| التعليقات: | Comment in: Blood. 2004 Apr 15;103(8):3245. (PMID: 15070713) |
| المشرفين على المادة: | 0 (CD3 Complex) 0 (CD8 Antigens) 0 (DNA, Complementary) 0 (HLA-A Antigens) 0 (HLA-A*02:01 antigen) 0 (HLA-A2 Antigen) 0 (Peptides) 63231-63-0 (RNA) |
| تواريخ الأحداث: | Date Created: 20030628 Date Completed: 20031124 Latest Revision: 20231120 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1182/blood-2003-01-0150 |
| PMID: | 12829610 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0006-4971 |
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| DOI: | 10.1182/blood-2003-01-0150 |