دورية أكاديمية
The effect of adenovirus-IkappaBalpha transduction on the chemosensitivity of lung cancer cell line with resistance to cis-diamminedichloroplatinum(II)(cisplatin) and doxorubicin(adriamycin).
| العنوان: | The effect of adenovirus-IkappaBalpha transduction on the chemosensitivity of lung cancer cell line with resistance to cis-diamminedichloroplatinum(II)(cisplatin) and doxorubicin(adriamycin). |
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| المؤلفون: | Lee CT; Department of Internal Medicine and Lung Institute of Medical Research Center, Seoul National University College of Medicine, 28 Yongon-Dong, Chongno-Gu, Seoul 110-744, South Korea., Seol JY, Lee SY, Park KH, Han SJ, Yoo CG, Han SK, Shim YS, Kim YW |
| المصدر: | Lung cancer (Amsterdam, Netherlands) [Lung Cancer] 2003 Aug; Vol. 41 (2), pp. 199-206. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Scientific Publishers Country of Publication: Ireland NLM ID: 8800805 Publication Model: Print Cited Medium: Print ISSN: 0169-5002 (Print) Linking ISSN: 01695002 NLM ISO Abbreviation: Lung Cancer Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Limerick : Elsevier Scientific Publishers Original Publication: Amsterdam, The Netherlands : Elsevier, c1985- |
| مواضيع طبية MeSH: | Adenoviridae* , Drug Resistance, Neoplasm* , I-kappa B Proteins*, Cisplatin/*pharmacology , Doxorubicin/*pharmacology , Lung Neoplasms/*metabolism, Blotting, Western ; Cell Line, Tumor ; Drug Therapy, Combination ; Humans ; Luciferases/metabolism ; Lung Neoplasms/drug therapy ; Signal Transduction/drug effects |
| مستخلص: | Resistance to chemotherapeutic agents is the major reason for treatment failure of cancer chemotherapy. Some chemotherapeutic drugs induce the activation of NF-kappaB in cancer cells that results in their resistance to anticancer drugs. But the role of NF-kappaB in acquired resistance has not been well investigated. In this study, we transferred the "super-repressor" form of the NF-kappaB inhibitor by adenoviral vector (ad-IkappaBalpha) to human lung cancer cell lines with resistant to cisplatin (PC-14-DDP) and adriamycin (PC-14-ADR), and observed the sensitivity change. Electrophoretic mobility shift assay showed that ad-IkappaBalpha blocked the activation of NF-kappaB induced by cisplatin and adriamycin. Transduction with ad-IkappaBalpha restored the sensitivity of cisplatin and adriamycin resistant lung cancer cell lines (PC-14-DDP and PC-14-ADR) to a level compatible to the parental cell lines. Annexin-V analysis suggested that the enhancement of chemosensitivity was probably a result of the induction of apoptosis. These data demonstrated that ad-IkappaBalpha blockade of chemotherapeutic induced NF-kappaB activation increased apoptosis induction and the chemosensitivity of lung cancer cell lines with acquired resistance to cisplatin and adriamycin. Therefore, gene transfer of IkappaBalpha-SR seems to represent a new therapeutic strategy for the solution of low sensitivity and lung cancer resistance to anticancer drugs. |
| المشرفين على المادة: | 0 (I-kappa B Proteins) 80168379AG (Doxorubicin) EC 1.13.12.- (Luciferases) Q20Q21Q62J (Cisplatin) |
| تواريخ الأحداث: | Date Created: 20030723 Date Completed: 20031208 Latest Revision: 20190922 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/s0169-5002(03)00227-7 |
| PMID: | 12871783 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0169-5002 |
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| DOI: | 10.1016/s0169-5002(03)00227-7 |