دورية أكاديمية
أعرض في EDS

Aberrant expression of minichromosome maintenance protein-2 and Ki67 in laryngeal squamous epithelial lesions.

التفاصيل البيبلوغرافية
العنوان: Aberrant expression of minichromosome maintenance protein-2 and Ki67 in laryngeal squamous epithelial lesions.
المؤلفون: Chatrath P; MRC Cancer Cell Unit, Hutchison/MRC Research Centre, Hills Road, Cambridge CB2 2XZ, UK., Scott IS, Morris LS, Davies RJ, Rushbrook SM, Bird K, Vowler SL, Grant JW, Saeed IT, Howard D, Laskey RA, Coleman N
المصدر: British journal of cancer [Br J Cancer] 2003 Sep 15; Vol. 89 (6), pp. 1048-54.
نوع المنشور: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group on behalf of Cancer Research UK Country of Publication: England NLM ID: 0370635 Publication Model: Print Cited Medium: Print ISSN: 0007-0920 (Print) Linking ISSN: 00070920 NLM ISO Abbreviation: Br J Cancer Subsets: MEDLINE
أسماء مطبوعة: Publication: 2002- : London : Nature Publishing Group on behalf of Cancer Research UK
Original Publication: London, Lewis.
مواضيع طبية MeSH: Biomarkers, Tumor/*metabolism , Carcinoma, Squamous Cell/*metabolism , Ki-67 Antigen/*metabolism , Laryngeal Neoplasms/*metabolism , Nuclear Proteins/*metabolism, Antigens, Neoplasm/metabolism ; Biopsy ; Carcinoma in Situ/metabolism ; Carcinoma in Situ/pathology ; Carcinoma, Squamous Cell/pathology ; Cell Cycle ; DNA Replication ; Humans ; Hyperplasia/metabolism ; Hyperplasia/pathology ; Immunoenzyme Techniques ; Laryngeal Neoplasms/pathology ; Minichromosome Maintenance Complex Component 2 ; Precancerous Conditions/metabolism ; Precancerous Conditions/pathology
مستخلص: Histological classification of laryngeal epithelial lesions is highly subjective, and methods of cytological detection are not well developed. Improved determination of aberrant cell cycle entry may allow increased objectivity in histological assessment and enable the development of less invasive diagnostic cytology tests. Sections of normal larynx (n=10), laryngeal dysplasia (n=20) and laryngeal squamous cell carcinoma (SCC) (n=10) were classified according to the Ljubljana classification and stained for markers of cell cycle entry, minichromosome maintenance protein-2 (Mcm-2) and Ki67. Expression patterns were compared using double labelling confocal microscopy. There was a correlation between Mcm-2 and Ki67 labelling indices (rho=0.93; 95% CI [0.84, 0.97]) and both markers showed increased expression from normal epithelium to SCC (Mcm-2, P=0.001; Ki67, P=0.0002). Importantly, there was minimal expression of Mcm-2 or Ki67 in the most superficial layers of normal larynx and abnormal or atypical hyperplasia, in contrast to carcinoma in situ and SCC. Clusters of Mcm-2/5-positive cells were present in cytological preparations from SCC, but not from those showing atypical hyperplasia or inflammation in non-neoplastic tissue. Minichromosome maintenance protein-2 staining may increase the objectivity and reliability of histological grading of laryngeal epithelial lesions. Laryngeal brushings, combined with immuno-enhanced liquid-based cytology, could be useful, as a less invasive approach, to the detection of laryngeal malignant and premalignant lesions.
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المشرفين على المادة: 0 (Antigens, Neoplasm)
0 (Biomarkers, Tumor)
0 (Ki-67 Antigen)
0 (Nuclear Proteins)
EC 3.6.4.12 (Minichromosome Maintenance Complex Component 2)
تواريخ الأحداث: Date Created: 20030911 Date Completed: 20031017 Latest Revision: 20181113
رمز التحديث: 20240829
مُعرف محوري في PubMed: PMC2376943
DOI: 10.1038/sj.bjc.6601234
PMID: 12966424
قاعدة البيانات: MEDLINE
الوصف
تدمد:0007-0920
DOI:10.1038/sj.bjc.6601234