دورية أكاديمية
Additive effect of TRAIL and p53 gene transfer on apoptosis of human lung cancer cell lines.
| العنوان: | Additive effect of TRAIL and p53 gene transfer on apoptosis of human lung cancer cell lines. |
|---|---|
| المؤلفون: | Jang SH; Department of Internal Medicine and Lung Institute of Medical Research Center, Seoul National University College of Medicine, Clinical Research Institute, Seoul National University Hospital, Seoul, Korea., Seol JY, Kim CH, Yoo CG, Kim YW, Han SK, Shim YS, Lee CT |
| المصدر: | International journal of molecular medicine [Int J Mol Med] 2004 Jan; Vol. 13 (1), pp. 181-6. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: D.A. Spandidos Country of Publication: Greece NLM ID: 9810955 Publication Model: Print Cited Medium: Print ISSN: 1107-3756 (Print) Linking ISSN: 11073756 NLM ISO Abbreviation: Int J Mol Med Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Athens, Greece : D.A. Spandidos, [1998- |
| مواضيع طبية MeSH: | Apoptosis/*physiology , Lung Neoplasms/*metabolism , Membrane Glycoproteins/*genetics , Tumor Necrosis Factor-alpha/*genetics , Tumor Suppressor Protein p53/*genetics, Adenoviridae ; Apoptosis Regulatory Proteins ; Genetic Vectors ; Humans ; Membrane Glycoproteins/metabolism ; Receptors, TNF-Related Apoptosis-Inducing Ligand ; Receptors, Tumor Necrosis Factor/metabolism ; TNF-Related Apoptosis-Inducing Ligand ; Transduction, Genetic ; Tumor Necrosis Factor-alpha/metabolism ; Tumor Suppressor Protein p53/metabolism |
| مستخلص: | TRAIL is a cytokine that can induce tumor-specific apoptosis through its specific death receptors (DR4 and DR5) and p53 has been proven to increase the expression of death receptors. To examine their interaction in tumor suppression, p53 and TRAIL genes were inserted in recombinant adenovirus vectors and transferred simultaneously into non-small cell lung cancer cell lines (NCI-H157, NCI-H358, NCI-H460 and A549). Western blot assay demonstrated production of TRAIL protein in NCI-H157 and A549 cell lines. Increased expressions of DR4 and DR5 of NCI-H157 and DR4 of A549 after p53 overexpression were confirmed by flow cytometry. p53 or TRAIL gene transfer increased sub-G1 fraction in cell cycle analysis and inhibited the tumor growth dose-dependently and the degree was potentiated by co-transfer. But isobologram analysis indicated an additive effect. Together, these data indicate that p53 and TRAIL interact additively on tumor apoptosis despite theoretical synergism. |
| المشرفين على المادة: | 0 (Apoptosis Regulatory Proteins) 0 (Membrane Glycoproteins) 0 (Receptors, TNF-Related Apoptosis-Inducing Ligand) 0 (Receptors, Tumor Necrosis Factor) 0 (TNF-Related Apoptosis-Inducing Ligand) 0 (TNFRSF10A protein, human) 0 (TNFRSF10B protein, human) 0 (TNFSF10 protein, human) 0 (Tumor Necrosis Factor-alpha) 0 (Tumor Suppressor Protein p53) |
| تواريخ الأحداث: | Date Created: 20031205 Date Completed: 20040316 Latest Revision: 20061115 |
| رمز التحديث: | 20231215 |
| PMID: | 14654992 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1107-3756 |
|---|