دورية أكاديمية
Elevation of hippocampal MMP-3 expression and activity during trauma-induced synaptogenesis.
| العنوان: | Elevation of hippocampal MMP-3 expression and activity during trauma-induced synaptogenesis. |
|---|---|
| المؤلفون: | Kim HJ; Department of Anatomy and Neurobiology, School of Medicine, Virginia Commonwealth University Medical Center, Medical Sciences Building Room #736, 1217 E. Marshall Street, PO Box 980709, Richmond, VA 23298, USA., Fillmore HL, Reeves TM, Phillips LL |
| المصدر: | Experimental neurology [Exp Neurol] 2005 Mar; Vol. 192 (1), pp. 60-72. |
| نوع المنشور: | Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 0370712 Publication Model: Print Cited Medium: Print ISSN: 0014-4886 (Print) Linking ISSN: 00144886 NLM ISO Abbreviation: Exp Neurol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Orlando Fl : Academic Press Original Publication: New York. |
| مواضيع طبية MeSH: | Brain Injuries/*enzymology , Hippocampus/*enzymology , Hippocampus/*injuries , Matrix Metalloproteinase 3/*metabolism , Neuronal Plasticity/*physiology , Presynaptic Terminals/*enzymology , Up-Regulation/*physiology, Animals ; Astrocytes/enzymology ; Brain Injuries/pathology ; Brain Injuries/physiopathology ; Denervation/adverse effects ; Disease Models, Animal ; Dizocilpine Maleate/pharmacology ; Entorhinal Cortex/injuries ; Entorhinal Cortex/physiopathology ; Gene Expression Regulation, Enzymologic/physiology ; Glial Fibrillary Acidic Protein/metabolism ; Gliosis/enzymology ; Gliosis/etiology ; Gliosis/physiopathology ; Hippocampus/pathology ; Immunohistochemistry ; Male ; Matrix Metalloproteinase 3/genetics ; Neural Pathways/injuries ; Neural Pathways/physiopathology ; Neuroprotective Agents/pharmacology ; RNA, Messenger/metabolism ; Rats ; Rats, Sprague-Dawley |
| مستخلص: | The matrix metalloproteinase (MMP) enzyme family contributes to the regulation of a variety of brain extracellular matrix molecules. In order to assess their role in synaptic plasticity following traumatic brain injury (TBI), we compared expression of stromelysin-1 (MMP-3) protein and mRNA in two rodent models of TBI exhibiting different levels of recovery: adaptive synaptic plasticity following central fluid percussion injury and maladaptive synaptic plasticity generated by combined TBI and bilateral entorhinal cortical lesion (TBI + BEC). We sampled the hippocampus at 7 days postinjury, targeting a selectively vulnerable brain region and a survival interval exhibiting rapid synaptogenesis. We report elevated expression of hippocampal MMP-3 mRNA and protein after TBI. MMP-3 immunohistochemical staining showed increased protein levels relative to sham-injured controls, primarily localized to cell bodies within the deafferented dendritic laminae. Injury-related differences in MMP-3 protein were also observed. TBI alone elevated MMP-3 immunobinding over the stratum lacunosum moleculare (SLM), inner molecular layer and hilus, while TBI + BEC generated more robust increases in MMP-3 reactivity within the deafferented SLM and dentate molecular layer (DML). Double labeling with GFAP confirmed the presence of MMP-3 within reactive astrocytes induced by each injury model. Semi-quantitative RT-PCR revealed that MMP-3 mRNA also increased after each injury, however, the combined insult induced a much greater elevation than fluid percussion alone: 1.9-fold vs. 79%, respectively. In the TBI + BEC model, MMP-3 up-regulation was spatio-temporally correlated with increased enzyme activity, an effect which was attenuated with the neuroprotective compound MK-801. These results show that distinct pathological conditions elicited by TBI can differentially affect MMP-3 expression during reactive synaptic plasticity. Notably, these effects are both transcriptional and translational and are correlated with functionally active enzyme. |
| معلومات مُعتمدة: | NS44372 United States NS NINDS NIH HHS |
| المشرفين على المادة: | 0 (Glial Fibrillary Acidic Protein) 0 (Neuroprotective Agents) 0 (RNA, Messenger) 6LR8C1B66Q (Dizocilpine Maleate) EC 3.4.24.17 (Matrix Metalloproteinase 3) |
| تواريخ الأحداث: | Date Created: 20050209 Date Completed: 20050414 Latest Revision: 20131121 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.expneurol.2004.10.014 |
| PMID: | 15698619 |
| قاعدة البيانات: | MEDLINE |
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