دورية أكاديمية
Differential role of hydrogen peroxide and staurosporine in induction of cell death in glioblastoma cells lacking DNA-dependent protein kinase.
| العنوان: | Differential role of hydrogen peroxide and staurosporine in induction of cell death in glioblastoma cells lacking DNA-dependent protein kinase. |
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| المؤلفون: | Chen GG; Department of Surgery, The Chinese University of Hong Kong, Prince of Wales Hospital, Shatin, N.T., Hong Kong. gchen@cuhk.edu.hk, Sin FL, Leung BC, Ng HK, Poon WS |
| المصدر: | Apoptosis : an international journal on programmed cell death [Apoptosis] 2005 Jan; Vol. 10 (1), pp. 185-92. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Country of Publication: Netherlands NLM ID: 9712129 Publication Model: Print Cited Medium: Print ISSN: 1360-8185 (Print) Linking ISSN: 13608185 NLM ISO Abbreviation: Apoptosis Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2005- : Dordrecht, Netherlands : Springer Original Publication: London : Rapid Science Publishers, |
| مواضيع طبية MeSH: | Brain Neoplasms/*metabolism , Cell Death/*drug effects , DNA-Binding Proteins/*genetics , Glioblastoma/*metabolism , Hydrogen Peroxide/*pharmacology , Protein Serine-Threonine Kinases/*genetics , Staurosporine/*pharmacology, Apoptosis ; Brain Neoplasms/genetics ; Brain Neoplasms/pathology ; DNA-Activated Protein Kinase ; DNA-Binding Proteins/deficiency ; DNA-Binding Proteins/metabolism ; Enzyme Inhibitors/pharmacology ; Glioblastoma/genetics ; Glioblastoma/pathology ; Humans ; Necrosis ; Nuclear Proteins ; Oxidants/pharmacology ; Protein Serine-Threonine Kinases/deficiency ; Protein Serine-Threonine Kinases/metabolism |
| مستخلص: | Various DNA double-strand break repair mechanisms, in which DNA-dependent protein kinase (DNA-PK) has a major role, are involved both in the development and treatment of glioblastoma. The aim of the present study was to investigate how glioblastoma cells responded to hydrogen peroxide and staurosporine (STS) and how such a response is related to DNA-PK. Two human glioblastoma cell lines, M059J cells that lack DNA-PK activity, and M059K cells that express a normal level of DNA-PK, were exposed to hydrogen peroxide or STS. The response of the cells to hydrogen peroxide or STS was recorded by measuring cell death, which was detected by three different methods-MTT, annexin-V and propidium iodide staining, and JC-1 mitochondrial probe. The result showed that both hydrogen peroxide and STS were able to induce cell death of the glioblastoma cells and that the former was mainly associated with necrosis and the latter with apoptosis. Glioblastoma cells lacking DNA-PK were less sensitive to STS treatment than those containing DNA-PK. However, DNA-PK had no significant influence on hydrogen peroxide treatment. We further found that catalase, an antioxidant enzyme, could prevent cell death induced by hydrogen peroxide but not by STS, suggesting that the pathways leading to cell death by hydrogen peroxide and STS are different. We conclude that hydrogen peroxide and STS have differential effects on cell death of glioblastoma cells lacking DNA-dependent protein kinase. Such differential roles in the induction of glioblastoma cell death can be of significant value in selecting and/or optimizing the treatment for this malignant brain tumor. |
| المشرفين على المادة: | 0 (DNA-Binding Proteins) 0 (Enzyme Inhibitors) 0 (Nuclear Proteins) 0 (Oxidants) BBX060AN9V (Hydrogen Peroxide) EC 2.7.11.1 (DNA-Activated Protein Kinase) EC 2.7.11.1 (PRKDC protein, human) EC 2.7.11.1 (Protein Serine-Threonine Kinases) H88EPA0A3N (Staurosporine) |
| تواريخ الأحداث: | Date Created: 20050216 Date Completed: 20050606 Latest Revision: 20211203 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1007/s10495-005-6073-8 |
| PMID: | 15711934 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1360-8185 |
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| DOI: | 10.1007/s10495-005-6073-8 |