دورية أكاديمية
أعرض في EDS

Functional analysis of the pyrimidine de novo synthesis pathway in solanaceous species.

التفاصيل البيبلوغرافية
العنوان: Functional analysis of the pyrimidine de novo synthesis pathway in solanaceous species.
المؤلفون: Schröder M; Botanisches Institut, Im Neuenheimer Feld 360, Ruprecht-Karls-Universität Heidelberg, 69120 Heidelberg, Germany., Giermann N, Zrenner R
المصدر: Plant physiology [Plant Physiol] 2005 Aug; Vol. 138 (4), pp. 1926-38. Date of Electronic Publication: 2005 Jul 15.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: American Society of Plant Biologists Country of Publication: United States NLM ID: 0401224 Publication Model: Print-Electronic Cited Medium: Print ISSN: 0032-0889 (Print) Linking ISSN: 00320889 NLM ISO Abbreviation: Plant Physiol Subsets: MEDLINE
أسماء مطبوعة: Publication: : [Rockville, MD] : American Society of Plant Biologists
Original Publication: Lancaster, Pa., American Society of Plant Physiologists.
مواضيع طبية MeSH: Pyrimidines/*biosynthesis , Solanum tuberosum/*enzymology , Nicotiana/*enzymology, Aspartate Carbamoyltransferase/genetics ; Aspartate Carbamoyltransferase/metabolism ; Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)/genetics ; Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)/metabolism ; Dihydroorotase/genetics ; Dihydroorotase/metabolism ; Dihydroorotate Oxidase/genetics ; Dihydroorotate Oxidase/metabolism ; Gene Expression Regulation, Plant ; Models, Chemical ; Molecular Sequence Data ; Molecular Structure ; Multienzyme Complexes/genetics ; Multienzyme Complexes/metabolism ; Orotate Phosphoribosyltransferase/genetics ; Orotate Phosphoribosyltransferase/metabolism ; Orotidine-5'-Phosphate Decarboxylase/genetics ; Orotidine-5'-Phosphate Decarboxylase/metabolism ; Plant Leaves/genetics ; Plant Leaves/physiology ; Plant Roots/genetics ; Plant Roots/physiology ; Plant Shoots/genetics ; Plant Shoots/physiology ; Plants, Genetically Modified ; Pyrimidines/chemistry ; Species Specificity
مستخلص: Pyrimidines are particularly important in dividing tissues as building blocks for nucleic acids, but they are equally important for many biochemical processes, including sucrose and cell wall polysaccharide metabolism. In recent years, the molecular organization of nucleotide biosynthesis in plants has been analyzed. Here, we present a functional analysis of the pyrimidine de novo synthesis pathway. Each step in the pathway was investigated using transgenic plants with reduced expression of the corresponding gene to identify controlling steps and gain insights into the phenotypic and metabolic consequences. Inhibition of expression of 80% based on steady-state mRNA level did not lead to visible phenotypes. Stepwise reduction of protein abundance of Asp transcarbamoylase or dihydro orotase resulted in a corresponding inhibition of growth. This was not accompanied by pleiotropic effects or by changes in the developmental program. A more detailed metabolite analysis revealed slightly different responses in roots and shoots of plants with decreased abundance of proteins involved in pyrimidine de novo synthesis. Whereas in leaves the nucleotide and amino acid levels were changed only in the very strong inhibited plants, the roots show a transient increase of these metabolites in intermediate plants followed by a decrease in the strong inhibited plants. Growth analysis revealed that elongation rates and number of organs per plant were reduced, without large changes in the average cell size. It is concluded that reduced pyrimidine de novo synthesis is compensated for by reduction in growth rates, and the remaining nucleotide pools are sufficient for running basic metabolic processes.
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سلسلة جزيئية: GENBANK AJ319872; AJ319873; AJ319874; AX093580; AX093582; U22260
المشرفين على المادة: 0 (Multienzyme Complexes)
0 (Pyrimidines)
74870-74-9 (uridine 5'-monophosphate synthase)
EC 1.3.98.1 (Dihydroorotate Oxidase)
EC 2.1.3.2 (Aspartate Carbamoyltransferase)
EC 2.4.2.10 (Orotate Phosphoribosyltransferase)
EC 3.5.2.3 (Dihydroorotase)
EC 4.1.1.23 (Orotidine-5'-Phosphate Decarboxylase)
EC 6.3.5.5 (Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing))
تواريخ الأحداث: Date Created: 20050719 Date Completed: 20051223 Latest Revision: 20240314
رمز التحديث: 20240314
مُعرف محوري في PubMed: PMC1183384
DOI: 10.1104/pp.105.063693
PMID: 16024685
قاعدة البيانات: MEDLINE
الوصف
تدمد:0032-0889
DOI:10.1104/pp.105.063693