دورية أكاديمية
Phosphatidylinositol 3-kinase C2alpha is essential for ATP-dependent priming of neurosecretory granule exocytosis.
| العنوان: | Phosphatidylinositol 3-kinase C2alpha is essential for ATP-dependent priming of neurosecretory granule exocytosis. |
|---|---|
| المؤلفون: | Meunier FA; Lincoln's Inn Fields Laboratories, London Research Institute, Cancer Research UK, London WC2A 3PX, United Kingdom., Osborne SL, Hammond GR, Cooke FT, Parker PJ, Domin J, Schiavo G |
| المصدر: | Molecular biology of the cell [Mol Biol Cell] 2005 Oct; Vol. 16 (10), pp. 4841-51. Date of Electronic Publication: 2005 Jul 29. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society for Cell Biology Country of Publication: United States NLM ID: 9201390 Publication Model: Print-Electronic Cited Medium: Print ISSN: 1059-1524 (Print) Linking ISSN: 10591524 NLM ISO Abbreviation: Mol Biol Cell Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Bethesda, MD : American Society for Cell Biology, c1992- |
| مواضيع طبية MeSH: | Adenosine Triphosphate/*metabolism , Exocytosis/*physiology , Neurosecretion/*physiology , Phosphatidylinositol 3-Kinases/*metabolism , Phosphatidylinositol Phosphates/*metabolism , Secretory Vesicles/*physiology, Adrenal Glands/cytology ; Animals ; Catecholamines/metabolism ; Cattle ; Cells, Cultured ; Chromaffin Cells/physiology ; Class II Phosphatidylinositol 3-Kinases ; Human Growth Hormone/metabolism ; Humans ; Mutation ; Phosphatidylinositol 3-Kinases/genetics ; Rats |
| مستخلص: | Neurotransmitter release and hormonal secretion are highly regulated processes culminating in the calcium-dependent fusion of secretory vesicles with the plasma membrane. Here, we have identified a role for phosphatidylinositol 3-kinase C2alpha (PI3K-C2alpha) and its main catalytic product, PtdIns3P, in regulated exocytosis. In neuroendocrine cells, PI3K-C2alpha is present on a subpopulation of mature secretory granules. Impairment of PI3K-C2alpha function specifically inhibits the ATP-dependent priming phase of exocytosis. Overexpression of wild-type PI3K-C2alpha enhanced secretion, whereas transfection of PC12 cells with a catalytically inactive PI3K-C2alpha mutant or a 2xFYVE domain sequestering PtdIns3P abolished secretion. Based on these results, we propose that production of PtdIns3P by PI3K-C2alpha is required for acquisition of fusion competence in neurosecretion. |
| References: | Nature. 1993 Dec 9;366(6455):572-5. (PMID: 8255295) J Neurosci. 1993 Sep;13(9):3895-903. (PMID: 8366350) J Neurosci. 1994 Dec;14(12):7695-703. (PMID: 7996204) J Neurosci. 1995 Feb;15(2):1249-60. (PMID: 7869096) Trends Cell Biol. 2001 Mar;11(3):116-22. (PMID: 11306272) EMBO J. 2001 May 15;20(10):2424-34. (PMID: 11350931) Curr Opin Neurobiol. 2001 Jun;11(3):297-305. (PMID: 11399427) Nature. 1995 Mar 9;374(6518):173-7. (PMID: 7877690) J Physiol Paris. 1995;89(2):83-94. (PMID: 8520575) EMBO J. 1996 May 1;15(9):2094-101. (PMID: 8641275) Proc Natl Acad Sci U S A. 1996 Nov 12;93(23):13327-32. (PMID: 8917590) J Cell Biol. 1997 Oct 20;139(2):339-49. (PMID: 9334339) Biochem J. 1997 Aug 15;326 ( Pt 1):139-47. (PMID: 9337861) Biochem Soc Trans. 1997 Nov;25(4):1137-41. (PMID: 9449963) J Neurochem. 1998 Jun;70(6):2347-56. (PMID: 9603199) J Biol Chem. 1998 Jul 3;273(27):16913-20. (PMID: 9642253) J Neurosci. 1998 Aug 1;18(15):5594-602. (PMID: 9671651) EMBO J. 1999 Jun 15;18(12):3293-304. (PMID: 10369670) Neuron. 1999 May;23(1):139-48. (PMID: 10402200) Br J Pharmacol. 1999 Sep;128(1):142-8. (PMID: 10498845) J Neurosci. 2005 Mar 9;25(10):2557-65. (PMID: 15758165) J Cell Biol. 2005 Jun 6;169(5):789-99. (PMID: 15928202) Nature. 2000 Mar 23;404(6776):355-62. (PMID: 10746715) Mol Cell Biol. 2000 Jun;20(11):3817-30. (PMID: 10805725) J Biol Chem. 2000 Jun 9;275(23):17878-85. (PMID: 10747966) Annu Rev Biochem. 1999;68:863-911. (PMID: 10872468) EMBO J. 2000 Jul 17;19(14):3586-96. (PMID: 10899113) EMBO J. 2000 Sep 1;19(17):4577-88. (PMID: 10970851) Traffic. 2000 Nov;1(11):892-903. (PMID: 11273133) J Cell Sci. 2001 Mar;114(Pt 6):1041-52. (PMID: 11228149) Mol Cell. 2001 Feb;7(2):443-9. (PMID: 11239472) Biochem J. 2001 Apr 15;355(Pt 2):249-58. (PMID: 11284710) Mol Biol Cell. 2001 Jun;12(6):1699-709. (PMID: 11408578) Neuron. 2001 Oct 11;32(1):9-12. (PMID: 11604134) Trends Cell Biol. 2001 Oct;11(10):404-5. (PMID: 11642229) Curr Biol. 2001 Oct 16;11(20):1636-42. (PMID: 11676927) Proc Natl Acad Sci U S A. 2001 Dec 18;98(26):15300-5. (PMID: 11752468) Sci STKE. 2001 Jan 16;2001(65):pe1. (PMID: 11752634) Nat Neurosci. 2002 Sep;5(9):835-6. (PMID: 12161757) Science. 2002 Oct 25;298(5594):781-5. (PMID: 12399579) J Cell Sci. 2002 Dec 1;115(Pt 23):4399-411. (PMID: 12414987) EMBO J. 2002 Dec 16;21(24):6733-43. (PMID: 12485994) J Neurosci. 2002 Dec 15;22(24):10680-9. (PMID: 12486161) Nature. 2003 Mar 13;422(6928):176-80. (PMID: 12634788) Physiol Rev. 2003 Apr;83(2):581-632. (PMID: 12663867) Proc Natl Acad Sci U S A. 2003 Apr 1;100(7):3995-4000. (PMID: 12646710) J Biol Chem. 2003 Aug 1;278(31):29065-71. (PMID: 12754199) Cell. 2003 Aug 8;114(3):299-310. (PMID: 12914695) Nat Struct Mol Biol. 2004 Jan;11(1):36-44. (PMID: 14718921) J Cell Biol. 1981 Oct;91(1):257-69. (PMID: 7298720) J Biol Chem. 1983 Apr 25;258(8):4989-93. (PMID: 6833287) J Cell Biol. 1985 Aug;101(2):667-76. (PMID: 4019588) J Biol Chem. 1987 Dec 5;262(34):16671-6. (PMID: 3680269) J Biol Chem. 1989 Apr 5;264(10):5412-9. (PMID: 2784433) J Biol Chem. 1989 Jun 15;264(17):10299-308. (PMID: 2722869) Biochem J. 1990 May 15;268(1):15-25. (PMID: 2160809) J Biol Chem. 1992 Aug 15;267(23):16219-25. (PMID: 1644807) Cell. 1992 Sep 4;70(5):765-75. (PMID: 1516133) J Cell Biol. 1992 Oct;119(1):139-51. (PMID: 1527165) Neuron. 1994 Apr;12(4):707-16. (PMID: 7909233) |
| معلومات مُعتمدة: | United Kingdom WT_ Wellcome Trust |
| المشرفين على المادة: | 0 (Catecholamines) 0 (Phosphatidylinositol Phosphates) 0 (phosphatidylinositol 3-phosphate) 12629-01-5 (Human Growth Hormone) 8L70Q75FXE (Adenosine Triphosphate) EC 2.7.1.- (Phosphatidylinositol 3-Kinases) EC 2.7.1.137 (Class II Phosphatidylinositol 3-Kinases) |
| تواريخ الأحداث: | Date Created: 20050802 Date Completed: 20060214 Latest Revision: 20240526 |
| رمز التحديث: | 20240526 |
| مُعرف محوري في PubMed: | PMC1237087 |
| DOI: | 10.1091/mbc.e05-02-0171 |
| PMID: | 16055506 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1059-1524 |
|---|---|
| DOI: | 10.1091/mbc.e05-02-0171 |