دورية أكاديمية
Depot characteristics and biodistribution of interleukin-2 liposomes: importance of route of administration.
| العنوان: | Depot characteristics and biodistribution of interleukin-2 liposomes: importance of route of administration. |
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| المؤلفون: | Anderson PM; Department of Pediatrics, University of Minnesota, Minneapolis 55455., Katsanis E, Sencer SF, Hasz D, Ochoa AC, Bostrom B |
| المصدر: | Journal of immunotherapy : official journal of the Society for Biological Therapy [J Immunother (1991)] 1992 Jul; Vol. 12 (1), pp. 19-31. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
| اللغة: | English |
| بيانات الدورية: | Publisher: Raven Press Country of Publication: United States NLM ID: 9102704 Publication Model: Print Cited Medium: Print ISSN: 1053-8550 (Print) Linking ISSN: 10538550 NLM ISO Abbreviation: J Immunother (1991) Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: New York Ny : Raven Press Original Publication: New York, NY, U.S.A. : Raven Press, c1991-c1992. |
| مواضيع طبية MeSH: | Interleukin-2/*administration & dosage, Animals ; Cell Line ; Delayed-Action Preparations ; Drug Carriers ; Female ; Immunoenzyme Techniques ; Injections, Intraperitoneal ; Injections, Intravenous ; Injections, Subcutaneous ; Interleukin-2/pharmacokinetics ; Iodine Radioisotopes ; Liposomes ; Mice ; Mice, Inbred C57BL ; Recombinant Proteins/administration & dosage ; Recombinant Proteins/pharmacokinetics ; Thorax ; Tissue Distribution/physiology |
| مستخلص: | Due to rapid clearance of interleukin-2 (IL-2), it has had limited effective use as an in vivo immunostimulant. Current experimental and clinical protocols generally must utilize large doses, multiple injections, or continuous infusions of IL-2 in order to achieve significant immunostimulation, often at the expense of systemic toxicity. Therefore, the pharmacodynamics of IL-2 liposomes were investigated. IL-2 liposome incorporation efficiency was 80.4% (SD 5.5); vesicle diameter was 1.65 microns (SD 0.09) as determined by fluorescence-activated cell sorting (FACS). Both formulation (free cytokine vs. IL-2 liposomes) and route of administration were important variables in determination of the biodistribution and pharmacokinetic characteristics of IL-2. When free [125I]IL-2 was given i.v. to mice, only 6.5% was in the blood and 3% in liver and spleen 2 h after injection; on the other hand, at 2 h greater than 70% of i.v. [125I]IL-2 liposomes were detected in the blood, liver, spleen, and lungs. Mean i.v. elimination t1/2 from the blood of rats given 20 x 10(6) U/kg free cytokine or IL-2 liposomes was 41 versus 102 min, respectively, as measured by bioassay and 59 and 119 min as measured by enzyme immunoassay (EIA). After i.v. administration, the estimated Vd of IL-2 liposomes was 13-fold smaller than the free cytokine. Intrathoracic (i.tx.), i.p., and s.c. administration of [125I]IL-2 to mice also demonstrated significant depot effects when IL-2 was incorporated into liposomes. These data suggest IL-2 liposomes may provide in vivo immunostimulation superior to the free cytokine due to biodistribution and depot characteristics. |
| معلومات مُعتمدة: | IR29-CA53517-01 United States CA NCI NIH HHS |
| المشرفين على المادة: | 0 (Delayed-Action Preparations) 0 (Drug Carriers) 0 (Interleukin-2) 0 (Iodine Radioisotopes) 0 (Liposomes) 0 (Recombinant Proteins) |
| تواريخ الأحداث: | Date Created: 19920701 Date Completed: 19920831 Latest Revision: 20151119 |
| رمز التحديث: | 20221208 |
| PMID: | 1637781 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1053-8550 |
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